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IPPN – A Global & Independent Network
This is a training material from the workshop for IFVCNS researchers titled Non-invasive plant phenotyping approaches and research infrastructure management, held on 4-5 March 2024 at FZJ within CROPINNO project. The material covers the organization, members, board and office of IPPN Plant Phenotyping Network, working groups, outreach, services and symposia.Workshop program, report and pictures [https://fiver.ifvcns.rs/handle/123456789/5598
Low-cost phenotyping approaches
This is a training material from the workshop for IFVCNS researchers titled Non-invasive plant phenotyping approaches and research infrastructure management, held on 4-5 March 2024 at FZJ within CROPINNO project. The material covers which environment and traits can be measured, at which throughput/frequency, accuracy and precision, how quickly data are processed and made available, and level of expertise required.Workshop program, report and pictures [https://fiver.ifvcns.rs/handle/123456789/5598
On Science Management
This is a training material from the workshop for IFVCNS researchers titled Non-invasive plant phenotyping approaches and research infrastructure management, held on 4-5 March 2024 at FZJ within CROPINNO project. The material covers System Thinking, Strategy & Strategic management, tools supporting stakeholder engagement, and project examples.Workshop program, report and pictures [https://fiver.ifvcns.rs/handle/123456789/5598
Spanish cane production in the function of circular economy and renewable energy sources
In recent years many countries direct their researches to finding the best way for getting energy and reasonable using of renewable energy as part of circular economy. In research it was analyzed a significant source of all forms of energy, the Spanish cane. Spanish cane can be used on many ways: as biogas, vegetable coal, and as an alternative source of plant fibers. Its cellulose fiber is used for production of paper as well as in the textile industry (substitute for cotton, flax, and kenaf fibers). The Spanish cane can be established as perennial grass areas at degraded and unused land due to its ability to be cultivated in marginal agro-ecological conditions. By development of new technologies for the biological waste processing into energy, within the circular economy, there is increase in the alternative fuel use and, thanks to its low requirements for water and warmth Spanish cane is a desirable energy crop. Speaking of erosion, it has to be mentioned that Spanish cane is one of the best crops for soil protection against all types of erosion. It is also very good in phytoremediation and absorption of harmful gases from the atmosphere, valued in as an ornamental plant and in the construction industry
Phenolic profile of Serbian old wheat local landraces and locally adapted varieties
Wheat is one of the most important staple crops in Serbia and plays an important role in food
security and nutrition. Phenolic compounds are dominant antioxidants in whole grains and are
essential quality traits in future breeding programs as they are known for their beneficial
cffects on health. With the aim of analyzing local wheat diversity in terms of phenolic
compounds, 33 local landraces and old locally adapted varieties and 3 modern wheat varieties
were grown in 2021/2022. So, the objective of this work was to optimize the extraction of
phenolic compounds and analyze them by HPLC/DAD method and compare old with modern
cultivars. As it is known that cereal grains contain different phenolic compounds, mainly
phenolic acids in bound form, the extraction of phenolic compounds was done from
wholemeal flour samples after hydrolysis. Alkaline hydrolysis conducted at 25°C for 12 h
using a 2 M NaOH solution in water demonstrated greater efficiency compared to employing
solutions of up to 10 M KOH in methanol under the same temperature and duration. HPLCDAD analysis revealed that ferulic acid presents the major phenolic compound both in old
and modern wheat samples ranging between 14 and 149 mg/kg in old and 51 and 61 mg/kg in
modern varieties. Apart from ferulic acid, vanillic acid (3.1-11.5 mg/kg), syringic acid
(1.4-5.5 mg/kg) and p-coumaric acid (1-6.9 mg/kg) were identified and quantified in old, but
not in new varieties. Our results indicate that the genetic potential of old varieties in terms of
polyphenolic compounds is not fully utilized because the content of ferulic acid in whole
grains in some samples is almost three times higher than in modern varieties that do not differ
significantly among themselves
First Report of Brown Spot on Stored Apple Fruits Caused by Stemphylium vesicarium in Serbia
Apple is one of the most economically important fruit crops worldwide, and postharvest fungal diseases can cause significant losses during storage (Petreˇs et al. 2020). Apple fruits (cv. Fuji) with necrosis symptoms were collected during the fall of 2022 from an ultra-low-oxygen cold storage facility in Titel, Serbia. The fruits originated from the apple orchards in Titel (45°12947.10N, 20°15923.60E). The pathogens were isolated from collected fruit samples using standard phytopathological techniques. Fruits were surface-sterilized, rinsed with sterile water, and aseptically cut in half, and small fragments collected from the border of healthy and diseased tissue were placed on potato dextrose agar (PDA) medium in Petri dishes and incubated at 25 ± 1°C in the dark for 7 days. The obtained 11 isolates were identified to the genus level as Alternaria (incidence, 46%), Penicillium (36%), Fusarium (9%), and Stemphylium (9%) based on their morphological characteristics. Pathogenicity of all the isolates was confirmed on apple fruits of cultivars Fuji and Golden Delicious. The fruits were surface-sterilized, sprayed with 5 ml of conidial suspension (1 × 105 conidia/ml), and incubated at room temperature for 21 days. Symptoms developed on inoculated fruits were the same as symptoms observed on apple fruit samples collected from cold storage
Phytoplasmas infecting sugar beet in central Europe
In Europe, the cultivation of sugar beet faces significant challenges due to the emergence of basses richesses syndrome (SBR) and rubbery taproot disease (RTD) which are associated with fastidious bacteria Candidatus Arsenophonus phytopathogenicus and Candidatus Phytoplasma solani, respectively. A comprehensive study conducted from 2018 to 2022 aimed to elucidate the prevalence and distribution of these pathogens in sugar beet in central Europe, focusing on the Pannonian Plain (particularly Serbia and Slovakia where RTD has been present in epidemic scales) and Germany
Transmission risks of beet yellows virus by Myzus persicae and Aphis fabae aphids in diverse experimental conditions
Sugar beet yields are negatively affected by the economically significant Beet yellows virus (BYV). For the past 30 years, effective control of BYV relied on neonicotinoid insecticides. However, the European Union's ban on outdoor use of these insecticides since 2018, driven by concerns over biodiversity and human health, led to widespread losses in the sugar beet industry due to BYV outbreaks. The main vectors of this virus are the green peach aphid (Myzus persicae) and the black bean aphid (Aphis fabae). Understanding the virusvector relationship is crucial for developing new control methods. To address this, our study investigates viral transmission efficiency in sugar beet plants based on aphid density, species, and morphs (winged/apterous) in laboratory, greenhouse, and field conditions
Organic soybean: ECOBREED partner contribution
One of the goals of the ECOBREED work package SOYBEAN was to develop soybean genotypes with improved agronomic performance and to offer know-how for cultivation practices tailor-made for low input and organic systems. During the project lifetime, the focus of activities was on henotyping for biotic (weed, pest, diseases) and abiotic (drought and chilling stress) stress tolerance screening, as well as crop and nutritive quality and N fixation efficiency. A marker-assisted selection programme was established to identify genes/QTL of particular importance for organic soybean varieties. In addition, an assessment of the effects of cover crops and inoculation in the process of soybean seed multiplication was performed. Creating and selecting new soybean lines suitable for organic production was a key priority. After the first half of the project and the conduction of different trials and identification of useful traits, cross composite populations (CCPs) and soybean lines were available for further field testing. Chosen soybean lines went through the registration trials (2021-2022) to evaluate their performance and adaptability. The first ECOBREED soybean variety was registered by IFVCNS. NS ECOB is the variety with high protein content (00 maturity group) specifically selected for organic and low input production requirements
Plant genetic resources and their use in organic agriculture
Organic agriculture and plant genetic resources are closely related topics, as organic farming relies on the conservation and use of diverse and adapted varieties of crops. Plant genetic resources are the raw materials that can help improve the productivity and quality of organic agriculture, as well as enhance its resilience to environmental stresses and pests. Organic agriculture also contributes to the in-situ conservation of plant genetic resources by maintaining and selecting local varieties that have a high degree of genetic variability. An important goal of the ECOBREED project is to identify genetic and phenotypic variations for morphological, abiotic/biotic tolerances/resistance and nutritional quality traits that can be used in organic breeding. The first step to achieve this goal was to make the inventory of available genotypes of four crops: wheat, potato, soybean, and buckwheat. For this purpose, we have examined genetic resources of four crops stored in gene banks, used in previous European and national research projects, and available through plant breeding programmes and seed companies. We have also used agronomic data on available and used varieties in organic farming, varieties characterized by good disease resistance and quality. Selected 200 wheat genotypes originate from 12 different European countries, 197 potato accessions were identified from several databases, the list contains 242 soybean accessions that originated all around the world and of buckwheat were identified through international databases. Much of the material represents cultivars that were released in the last two decades and are included in either the European list or national lists