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Molecular cloning of Indian hedgehog gene and its expression in golden pompano Trachinotus ovatus (Linnaeus 1758) larvae at different water temperatures
Get PDFThe Indian hedgehog (Ihh) gene in golden pompano larvae was cloned and analyzed. The expression of Ihh during larval fish ontogeny in the first 18 days was examined, and then the expression of Ihh in fish tissues was evaluated on 18 days post hatching (DPH). Subsequently, the response of Ihh to water temperatures of 23, 26, and 290 C was compared on 12 DPH and 18 DPH. The cDNA sequence length of the golden pompano Ihh gene is 1,484 bp with an open reading frame of 1,314 bp. The Ihh gene encodes 437 amino acids and has a calculated molecular weight of 48.175 kDa and a theoretical isoelectric point of 6.22. After hatching, the expression of Ihh increased with fish age and peaked at 3 DPH. After fluctuations between 4 and 12 DPH, the expression of Ihh reached the second peak at 18 DPH. The highest expression of Ihh in tissues occurred in the fish intestine, followed in the stomach on 18 DPH. Water temperature significantly affected Ihh expression. On 12 DPH, the expression of Ihh at 23oC was significantly higher than that at 26 and 29 oC. In contrast, the expression of Ihh at 26 and 290 C was significantly higher than that at 23 oC on 18 DPH. This study detected the gene expression of Ihh at the early stage of golden pompano development and the time-dependent expression of Ihh in fish larvae is important to understand the ontogeny of bone formation in fish larvae
"Far from their homeland, which artists need along with colors and inspiration": Russian artists in China
Get PDFInstitute for Research, Extension, and Training in Agriculture's South Pacific agricultural news
No full textAcinetobacter johnsonii: an Emerging Pathogen for Cultured Blunt Snout Bream Megalobrama amblycephala
Get PDFBacteriosis has caused significant economic losses in farmed blunt snout bream Megalobrama amblycephala. Only scarce information is available on Acinetobacter johnsonii as a possible causal agent in M. amblycephala. In this study, a virulent strain temporarily named BY3, was isolated from diseased bream, identified phenotypically and molecularly as A. johnsonii. Phylogenetic trees of 16S rRNA and gyrB gene sequences were constructed to examine the taxonomic position of isolate BY3 and compare it to other known isolates. When screened against a range of common veterinary antibiotics, isolate BY3 has not shown resistance to florfenicol, neomycin, and sulfisoxazole antibiotics in aquaculture. To the best of our knowledge, this is the first report of A. johnsonii infection in farmed blunt snout bream
On the Linguistic Affiliation of 'Tai Loi'
Get PDFThis short investigation of languages referred to as Tai Loi shows that at least seven different languages from three Palaungic subgroups are referred to by this exonym. Simply meaning ‘mountaineers’, Tai Loi appears to denote Buddhist speakers as a sociopolitical identity rather than a linguistic one. As a linguistic designation, it may lead to confusion and should therefore be avoided. Since ISO 639 forms a part of the language tags distinguishing dialectal, regional and script variation in languages, there is the potential for even broader confusion
Puka Puka Parade 2017 - Number 03
No full textDigital-only addendum included in same pdf file as main issue
Puka Puka Parade 2017 - Number 07
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