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Puka Puka Parade 2019 - Number 10
Digital-only addendum and an insert to the mailed PPP provided as separate files from the main issue
Undated Proclamation, U.S. Army, To the People of Hawaii from the Commanding General, Hawaiian Department and Military Governor of Hawaii
Undated Proclamation, U.S. Army, To the People of Hawaii from Lieutenant General Delos C. Emmons, Commanding General, Hawaiian Department and Military Governor of Hawaii, relinquishing specified jurisdiction and authority to civilian authorities and the courts of the Territory of Hawaii
Reference Material: Comments on Conclusions and Recommendations a Legislative Study
This folder holds comments on the conclusions and recommendations of the legislative study of the Island of Kaho'olawe. This paper also looks at the current status of the Navy's study of Kaho'olawe and looks at factors that hamper state of Hawai'i and Navy cooperation. Archaeological work is also mentioned, along with actions by the Navy to preserve or protect places of importance on Kaho'olawe
Effect of Dietary Iron Levels on Tissues, Intestinal Digestive Enzyme Activity, and Muscle Nutrient Compositions of Juvenile Bighead Carp (Aristichthys nobilis)
A 60-day feeding trial was conducted to estimate the effects of dietary iron (Fe) levels on iron concentration in tissues, intestinal digestive enzyme activities, and muscle nutrient composition of bighead carp (Aristichthys nobilis). Six experimental diets were formulated to contain different Fe levels (0, 43.1, 84.2, 123.3, 162.2 and 203.1 mg/kg of dry diets) using ferrous sulfate (FeSO4) as the source. When Fe dietary content increased to 43.1 mg/kg, trypsin activity in the intestine significantly increased and thereafter decreased. Lipase and amylase activity in the intestine significantly increased with increasing dietary Fe levels up to 123.3 mg/kg diet and thereafter decreased. With lipase and amylase activity in the intestine as the main indicators, Fe content of 123.3 mg/kg was the most suitable dietary Fe level for A. nobilis. Crude protein content in the muscle of the 84.2 mg/kg Fe group was the highest in all groups. Results indicated that the appropriate levels of dietary Fe alter muscle nutrient composition of A. nobilis. Fe content in the muscle and vertebrae significantly increased with increasing dietary Fe levels up to 203.1 mg/kg diet. Fe contents in different tissues were as follows: Vertebra >intestine >muscle
Notes & Queries: An Inquiry Into Kawaiaha'o Seminary’s “Melting Pot” Photograph Published in The National Geographic Magazine in 1924
Native Hawaiian Claims, 1974 - 1984: Hawaiian Native Claims Settlement Study Commission, Draft
NOT OCRed. This folder holds a draft of a speech in support of S.J. Res. 4, the Hawaiian Native Claims Study Commission Act
Citrobacter freundii: a causative agent for ulcer disease in snakehead fish Ophiocephalus argus (Cantor)
Ulcer disease has caused significant economic losses in the culture of snakehead fish Ophiocephalus argus (Cantor). Information available on Citrobacter freundii as a possible causal agent for this disease is scarce. In this study, a virulent strain, temporarily named GB1, was isolated from diseased snakehead fish suffering from ulcer disease and was identified as C. freundii through molecular and phenotypic methods. A phylogenetic tree was constructed to examine isolate GB1 and compare it to other known isolates. In addition, isolate GB1 has developed resistance to aminoglycosides, tetracyclines, and sulfonamides drugs for veterinary use in aquaculture as revealed when screened against a range of common antibiotics. To the best of our knowledge, this is the first report of ulcer disease caused by C. freundii in snakehead fish.
Publishe
Inhibition of Anabaena flos-aquae by Galla chinensis
Galla chinensis is a traditional herbal medicine in China with detoxification, bacteriostatic, and anti-inflammatory properties. In this study, we exposed Anabaena flos-aquae to different concentrations of Galla chinensis aqueous extracts. As the exposure concentrations and duration increased, chlorophyll a of Anabaena flos-aquae was significantly reduced. After 72 h of exposure, protein content, MDA content, and superoxide dismutase activity in Anabaena flos-aquae gradually decreased with increasing concentrations of Galla chinensis aqueous extracts. Electron microscopy for ultrastructural analysis showed that compared to the control group, as the concentration of Galla chinensis increased, the Anabaena flos-aquae cell walls exhibited plasmolysis with blurred basic structures in the cytoplasm, deformation of algal cells, collapse, and rupture of cell walls, and most cells died. Galla chinensis can effectively inhibit the growth of Anabaena flos-aquae and exhibits significant allelopathy