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Political Offices: U.S. House of Representatives: Federal Departments & Agencies: Dept. of Interior: Molokaʻi irrigation
The use of supplementary feed with ethanol extract of <em>Syzygium nervosum</em> leaf to prevent acute hepancreatic necrosis (AHPND) in white shrimp (<em>Litopenaeus vannamei</em>)
This study evaluated the effects of supplementing shrimp diets with an ethanol extract of Syzygium nervosum leaf on growth performance, survival rate, immune parameters, and resistance to AHPND in white shrimp (L. vannamei). Shrimp juveniles (weighing 1.05 ± 0.04 g) were fed diets supplemented with varying concentrations of S. nervosum leaf extract, including 12.5 g/kg (NT1), 25.0 g/kg (NT2), 37.5 g/kg (NT3), and a control diet without S. nervosum leaf extract (DC). After 28 days of feeding, the shrimp were challenged with Vibrio parahaemolyticus. The results showed no significant differences in growth performance and feed conversion ratio between the experimental groups and the control group (p>0.05). However, the survival rates of shrimp in the supplemented groups (93.4-94.1%) were significantly higher than those in the control group (90.0%) (pV. parahaemolyticus, the cumulative mortality of shrimp in the supplemented groups was significantly lower than that of the control group (pS. nervosum leaf extract at a concentration of 12.5 g/kg as the optimal level for practical application in white shrimp farming
Effects of water quality on the hatching of rapa whelk (<em>Rapana venosa</em>) eggs and larvae
To detect the effects of water quality factors on the hatching of Rapana venosa eggs, the eggs were exposed to controlled conditions with different concentrations of ammonia nitrogen and nitrite and different pH values respectively, for 60 days. The hatching rate, larval release time, and initial larvae sizes after hatching were documented and analyzed. Egg capsules had 100% mortality at pH ranges of 6.0-7.00 and 9.00-10.00, and the mortality rate was relatively low between pH of 7.50-8.50. With an increase in pH within this range, mortality decreased. Larval release times were also significantly affected by pH. Within the pH range of 7.50-8.50, the shortest larval release occurred at a pH of 8.5 and the longest at a pH of 7.5. Ammonia nitrogen had significant effects on larval body length and length to width ratio, with larval length increasing with decreasing ammonia nitrogen concentrations, whereas the ratio of length to width increased with decreasing ammonia nitrogen concentrations. Nitrite concentration had significant effects on the larval body length and ratio of length to width. In addition, pH had significant effects on larval body width and the length to width ratio. With increasing pH, larval body width decreased, whereas the ratio of length to width increased. This study explores the optimal growth conditions for the Rapana venosa, providing theoretical guidance for its industrial aquaculture and the conservation of wild resources
Empowering Indigenous Women in the Cordillera in support of land rights and indigenous women's dignity (unknown date of publication)
Puka Puka Parade 2025 - Volume 80, Number 06
Cover email sent with the born-digital PPP, showing the link to a youtube video related to the 100th Infantry Battalion, provided as a separate file from the main issue
Development of a microparticulate feed for the nursery rearing of the giant mottled eel (<em>Anguilla marmorata</em>)
This study was conducted to develop a microparticulate feed for the nursery culture of Anguilla marmorata. The developed crumble-type formulated feed was evaluated through two separate feeding trials. In the first trial, the growth performance of A. marmorata elvers fed with dry crumble-type feed was compared to those fed the conventional moist dough-type nursery diet over a 16-day period. The results revealed that elvers fed with the crumble-type feed exhibited significant growth performance, with a specific growth rate (SGR) of 1.57 and percent weight gain (%WG) of 24.62%, (pA. marmorata elvers, while offering practical advantages in terms of ease of management and feed preparation
Positive relationship between <em>Clostridium</em> abundance and bacterial community diversity in the aquaculture water of hybrid grouper (*Epinephelus fuscoguttatus*♀ × *E. lanceolatus*♂) in coastal areas of Hainan Island
The hybrid grouper, characterized by its accelerated growth rate, robust disease resistance, and superior nutritional-economic value, has emerged as a pivotal mariculture species in China and Southeast Asia. This study systematically revealed the microbial communities within aquaculture waters across five coastal regions of Wenchang, Wanning, Lingshui, Ledong, and Dongfang in Hainan Island, and elucidated potential interspecific interactions in microbiota. The α-diversity indices, principal coordinate analysis and β-diversity metrics demonstrated substantial variations in bacterial abundance, species diversity, and community structure. Conversely, fungal assemblages showed no statistically significant spatial differentiation. Taxonomic profiling identified Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, and Cyanobacteria as dominant bacterial phyla. Fungal communities were predominantly composed of Ascomycota and Basidiomycota. Notably, Clostridium abundance in the outdoor mariculture ponds exhibited positive correlations with key bacterial parameters, suggesting its potential as a bioindicator for microbial community dynamics. These findings advocate for precision management strategies when utilizing Clostridium-based probiotics in outdoor mariculture systems. We propose implementing a dose-response protocol to optimize probiotic supplementation thresholds and prevent dysbiosis-induced fluctuations in aquatic microbial diversity. This approach provides critical insights for microbiome engineering in industrialized grouper aquaculture systems
Gut Microbiota deliverables: reflecting the Efficacy of Dietary Probiotic Supplementation for Growth and Feed Utilization in <em>Litopenaeus vannamei</em> Towards Sustainable Aquaculture
The growing demand for sustainable aquaculture requires improvements in shrimp production efficiency and health management. This study investigated the effects of dietary Bacillus subtilis and Lactobacillus acidophilus supplementation on the gut microbiota dynamics, growth performance, survival, and feed utilization of the Pacific white shrimp (Litopenaeus vannamei) post-larvae. Shrimp were reared over 84 days under four experimental groups: control (a probiotic-free diet), supplemented with 10⁷ CFU/g Lactobacillus (L), 10⁷ CFU/g Bacillus (B), and a mixed probiotic treatment (M) with 0.5x10⁷ CFU/g per each. Growth metrics, survival rate and, feed utilization were assessed, and entire intestinal samples were analyzed using high throughput DNA sequencing of V1-V9 of 16S rRNA hypervariable regions. Bacillus supplementation significantly improved final body weight and specific growth rate (SGR) compared to the other groups (p 0.05). The mixed probiotic group demonstrated significantly better FCR than the Bacillus group (p Vibrio at the genus level (18.06%–48.28%). Neither alpha nor beta diversity was significantly affected by probiotic treatments (p > 0.05), though temporal and treatment-specific shifts in bacterial composition were detected. These results suggest that Bacillus and mixed probiotic supplementation may enhance shrimp growth and feed efficiency, supporting their potential application in sustainable shrimp farming
Reciprocal Constructions in Bodo
Bodo employs optional nominal reciprocals and obligatory reciprocal verbs to form reciprocal constructions. Usually, the nominal reciprocal that is derived from a pronoun is formed by fully reduplicating the reflexive pronoun [ɡao] ‘self’, and reduplicants are conjoined together with the associative case marker [-zɯŋ] as [ɡao-zɯŋ~ɡao] ‘each other; one another’. However, there are other forms of Bodo nominal reciprocals constructed using either numerals or nouns instead of pronouns. Reciprocal pronouns are common across Bodo-Garo languages (BG) of north-east India, whereas reciprocal nouns are very rare. On the other hand, reciprocal verbs employ the reciprocal suffix [-lai]