University of Hawaiʻi at Mānoa

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    The long-term effect of non-invasive sampling on the genetic diversity and growth performance of cultured <em>Gymnocypris chilianensis</em> (Cyprinidae) population: an evaluation based on 70 days

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    Non-invasive and non-lethal sampling methods are widely employed in conservation biology, but their long-term effects on sampled organisms remain understudied. To evaluate these impacts on fish and establish a scientific framework for ethical sampling practices, we conducted a dual investigation using the plateau-endemic species Gymnocypris chilianensis (Cyprinidae). First, genetic diversity in a cultured population was analyzed using non-invasive mucus sampling. Sequencing of mitochondrial Cytb (1098 bp) and D-loop (696 bp) regions revealed low genetic diversity, with Cytb showing six polymorphic sites, seven haplotypes (haplotype diversity H = 0.354; nucleotide diversity π = 0.0004), and D-loop displaying 10 polymorphic sites, 9 haplotypes (H = 0.787; π = 0.00315). The D-loop region demonstrated higher variability, supporting its utility as a marker for population genetic studies. Second, 180 individuals were divided into three groups, i.e., groups N (macus), Q (fin), and C (control), and monitored for 70 days to assess growth parameters (standard length, body weight), condition factor (K), and mortality. Generalized additive models (GAMs) revealed no significant differences in morphological indicators (p > 0.05) or mortality rates among groups, with a nonlinear standard length–weight regression relationship identified. Notably, all groups exhibited K values exceeding 200%, indicating excellent body condition. Comparative analyses confirmed minimal sequence divergence between non-invasive and traditional sampling methods, validating methodological reliability. This study provides the first long-term evaluation of non-invasive and non-lethal sampling impacts on fish growth, the results showed that non-invasive sampling had no significant impact on the growth or survival of fish, which was very important for future research and protection, combined with genetic diversity insights, offering critical guidelines for conserving vulnerable species like G. chilianensis in the Qinghai-Tibet Plateau

    Comparison of the potential effects of three compound probiotics on growth, intestinal microbiota, intestinal morphology, and immunity of juvenile turbot (<em>Scophthalmus maximus</em>)

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    A 36-day feeding trial was conducted to compare the effects of the diet addition of three commercial compound probiotics (Ma, Mb, Mc) on growth performance, intestinal health, and immunity of juvenile turbot (30-day-old, 0.15 ± 0.07 g). The results showed that adding probiotics to the diet can improve growth performance and the Ma and Mb group had the most significant facilitation effect (P OTUs richness and diversity, the relative abundance of Firmicutes and Bacteroidetes at the phylum level, and Lactobacillus at the genus level, with the significant enrichment of potential probiotics. Notably, the Ma group also significantly increases metabolism-related functions. Both probiotic groups increased the fish intestinal villus length. Moreover, after 14 days of challenge test with Aeromonas salmonicida, the cumulative mortality rate was the lowest and the time of death was the latest in the Ma group. In general, compared with the Mb and Mc group, supplementation of Ma to juvenile turbot could significantly improve growth, intestinal morphology, and immune ability with the promotion of potential probiotics in the host intestine

    A Pictogram from Anakuakala (Pāhoa Cave) in the Puna District, Hawai‘i Island

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    This paper describes a unique archaeological feature discovered during the emergency survey of a cave near Pāhoa Town on the Island of Hawaiʻi. The survey was undertaken during the 2014 eruption of Kīlauea Volcano. The feature is a pictogram in the form of a geometric design, and it is of unquestionable anthropogenic origin. Two radiocarbon dates suggest a late pre-contact origin. The feature consists of multiple elements, including slabs of rock and long rootlets collected from elsewhere in the cave. It is surrounded by patches of concentrated ash. The feature rests on a bare pāhoehoe floor in the dark recesses of the fortified cave. A case is made for the importance of the cave at the landscape level. The image is compared with the tradition of Hawaiian hei (string figures) and with a petroglyph from Kaho‘olawe Island, with which it shares graphic elements. These analogies, along with the culture-historical and physical context of the feature, are combined in an attempt to place it within a broader discussion of anthropological archaeology and social production in pre-contact Hawai‘i

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