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Tenacibaculum salmonis sp. nov., isolated from Atlantic salmon (Salmo salar L.) fish farmed in Chile
No full textIndexación: Scopus.Strain P3-BQ1T is a Gram-negative, aerobic, non-spore-forming bacterium with gliding motility and filamentous cells. It was isolated in 2018 from the gills of a diseased Atlantic salmon (Salmo salar) during an outbreak of tenacibaculosis in a Chilean fish farm. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain P3-BQ1T belongs to the genus Tenacibaculum and is most closely related to Tenacibaculum dicentrarchi 35/09T (98.53%), ‘Tenacibaculum pacificus’ 18-2881-A (98.46%), Tenacibaculum aestuariivivum JDTF-79T (98.46%), Tenacibaculum finnmarkense TNO010 (97.85%) and Tenacibaculum piscium TNO20T (97.41%). The genome size of strain P3-BQ1T is 2,777,603 bp, with a DNA G+C content of 29.18 mol%. The strain has an OrthoANI score lower than 95% compared to the types of all validly named Tenacibaculum species, with the closest neighbour being T. dicentrarchi 35/09T (score 87.41 %). In contrast, P3-BQ1ᵀ and ‘T. pacificus’ 182881-A shared a 96.58%, thus exceeding the species threshold. This result is also supported by whole-genome in silico DNA–DNA hybridization/Genome-to-Genome Distance Calculator values, indicating that strains P3-BQ1T and 18-2881-A are conspecific. Strain P3-BQ1T contains MK-6 as its sole detectable menaquinone. The polar lipids profile includes glycolipids (n=2), aminolipids (n=3) and unidentified lipids (n=5). The predominant cellular fatty acids (>5 %) are C13: 1, C15: 0, iso-C15: 0, iso-C15: 0 3-OH and summed feature 3 (C16: 1 ω7с/C16: 1 ω6с). An immersion challenge was conducted to assess the pathogenic potential in Atlantic salmon. Our findings indicate that strain P3-BQ1T alone does not cause significant mortality, suggesting it is likely non-pathogenic to this species. Based on the phenotypic, phylogenetic and genotypic data presented, Tenacibaculum salmonis sp. nov. is proposed here with type strain P3-BQ1T (=IMI 507635T =RGM 3581T), being 18-2881-A an additional strain. © 2025, Microbiology Society. All rights reserved.https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.006963Funding text
This work received financial funding from the Agencia Nacional de Investigaci\u00F3n y Desarrollo (ANID, Chile) through grants FONDECYT 1230068 and FONDAP 1523A0007.S
Teaching practices and reconstruction of pedagogical intervention based on classroom support models in rural and urban educational institutions in four regions of Colombia
No full textIndexación: Revista UNAB.Este artículo presenta los hallazgos principales de la investigación centralizada en la caracterización de las prácticas pedagógicas en contextos rurales y urbanos, así como en el análisis del acompañamiento pedagógico como estrategia para el fortalecimiento profesional docente. A partir de un enfoque cualitativo interpretativo, se desarrolló un estudio de caso múltiple en diversas regiones de Colombia, utilizando entrevistas, grupos focales y observación participante como técnicas principales. Los resultados evidencian que las prácticas docentes están profundamente determinadas por las condiciones territoriales y socioculturales, y que el acompañamiento resulta efectivo cuando se implementa desde una lógica horizontal, situada y colaborativa. Se destaca el papel del acompañamiento en la resignificación de la identidad docente, el fortalecimiento de la autonomía profesional y la generación de comunidades de aprendizaje. La investigación tiene como conclusión que el acompañamiento pedagógico debe asumirse como una política pública estructural, articulada al desarrollo profesional docente y orientada a la justicia educativa.This article presents the main findings of a research focused on the characterization of pedagogical practices in rural and urban contexts, as well as the analysis of pedagogical mentoring as a strategy for teacher professional development. Based on an interpretive qualitative approach, a multiple-case study was conducted in various regions of Colombia, using inter-views, focus groups, and participant observation as the main techniques. The results show that teaching practices are deeply determined by territorial and sociocultural conditions, and that mentoring is effective when implemented from a horizontal, situated, and collaborative perspective. The role of mentoring in redefining teacher identity, strengthening professional au-tonomy, and generating learning communities is highlighted. The research concludes that pedagogical mentoring should be assumed as a structural public policy, articulated with teacher professional development and oriented toward educational justice.S
Back to the Wastes: The Potential of Agri-Food Residues for Extracting Valuable Plant Cell Wall Polysaccharides
No full textIndexación Scopus.The agro-industrial sector generates large volumes of fruit waste each year, leading to environmental concerns and sustainability challenges. In this study, we evaluate the potential of fruit residues—apple, pear, blueberry, tomato, papaya, and a mixed fruit juice blend—as alternative sources of high-value polysaccharides, including pectins, hemicelluloses, and cellulose. Additionally, white strawberry, included as a reference from fresh fruit rather than agro-industrial waste, was analyzed to expand the comparative framework. These biopolymers, naturally derived from the plant cell wall, are renewable and biodegradable, and they possess physicochemical properties suitable for applications in food, pharmaceutical, cosmetic, textile, and bioenergy industries. Using a combination of cell wall fractionation, biochemical characterization, and immunodetection of specific structural domains, we identified significant variability in polysaccharide composition and structure among the samples. Blueberry, pear, and apple residues showed high levels of rhamnogalacturonan-I (RG-I) with extensive branching, while variations in rhamnogalacturonan-II (RG-II) dimerization and the degree of methylesterification of homogalacturonan were also observed. These structural differences are key to determining the gelling properties and functional potential of pectins. In the hemicellulose fractions, xylans and xyloglucans with distinct substitution patterns were especially abundant in apple and pear waste. Our findings demonstrate that fruit processing waste holds significant promise as a sustainable source of structurally diverse polysaccharides. These results support the reintegration of agro-industrial residues into production chains and emphasize the need for environmentally friendly extraction methods to enable industrial recovery and application. Overall, this study contributes to advancing a circular bioeconomy by transforming underutilized plant waste into valuable functional materials.https://www.mdpi.com/1422-0067/26/10/494
Block strength training based on age-related functional consequences in older women
No full textIndexación: Scopus.Strength training is a form of healthy ageing in older women. Although recommendations currently exist, some are very broad or fail to cover the needs of aging. Therefore, the purpose of this study was to analyze the effects of blocks strength training based on age-related functional consequences on functional performance in older adult women. 82 community-dwelling older women (70.17±6.04 y) were randomly assigned to either experimental (n=40) or control (n=42) group. Experimental group performed a Block Strength Training (BST) program based on strength, power, and muscular endurance, and different level of effort for 9 weeks, and control maintained daily routine with physical activity recommendations. Functional performance was assessed using absolute handgrip strength [AHS], timed up and go [TUG], two-minutes step test [2MST], five times stand-to-sit test [5-SST], 6-m walking speed test [6-WS] pre-post intervention. Statistical analyses were performed using two-way ANOVA (Time*Group) and effect size (partial eta-squared, ŋP2) with a significance level of p0.11). This BST is effective in improving overall functional performance and thus reducing the risk of physical frailty in community-dwelling older women. These findings strengthen the approach to exercise programming over recommendations, moving toward effective precision dosing for older adults. © 2025 Jofré-Saldía et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0323501Funding text
This research was funded by Villa San Jos\u00E9 senior adults club and the Chilean National Copper Corporation (CODELCO) Andean division (CONV 57). The listed funding agencies had no role in the design, data collection, analysis, or interpretation of the study.S
Three-dimensional exploration of the chicken embryo, a comparative study of light sheet and histological visualisation
No full textIndexación: Scopus.Ultramicroscopy has offered new avenues into the visualisation of tissues within animal models, providing three-dimensional visualisation through the use of light sheet fluorescence microscopy. This study aimed to develop and apply an optical tissue clearing method to investigate the application of light sheet fluorescence microscopy to image late-stage chicken embryos, and compare anatomical visualisation to traditional histological staining. Seventeen-day old specific pathogen free embryos were collected, fixed, and sectioned. Haematoxylin and eosin stained sections were prepared for histology, while light sheet imaging required the tissues to be optically clear. For this, an ethyl cinnamate-based method was utilised, allowing for acquisition of clear, unobstructed three-dimensional images of significant organ structures and systems using only autofluorescence. The use of established histological techniques provided anatomical mapping of structures between familiar histology images and the three-dimensional light sheet images. Rendering of organs using light sheet imaging provided contextual insights into the surrounding tissues and physiological architecture of major organ structures and systems. This was most apparent through the identification of the pulmonary vein and rendering of a volumetric projection of the vasculature branching within the lung and the subsequent merging of vasculature into the left side of the heart. Overall, the visualisation of the chicken embryo was enhanced by combining traditional histology with the information gained by three-dimensional light sheet fluorescence microscopy. © 2025 Smallridge et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0320483Funding text
The Melbourne Veterinary School Research Initiatives Fund was awarded Carol Hartley and Paola Vaz, who were the Chief Investigators for this project. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors would like to thank Ellie Cho, and Shane Doris Cheung from the Biological Optical Microscopy Platform at the University of Melbourne for their contributions to the acquisition and analysis methodologies for the light sheet fluorescence microscopy images. The authors would also like to thank Laura Leone and the Melbourne Histology Platform for sample preparation and sectioning, and Phenomics Australia for imaging the histology slides.S
Adaptação da escala de bem-estar social para professores e funcionários da escola
No full textIndexación: Scopus.Social well-being is the assessment of social functioning, which plays a key role in mental health. In the school context, teachers and school staff can promote students’ well-being, but it is related to their own. To assess their social well-being, the Keyes social well-being scale was adapted to the school context. Psychometric properties were analyzed in 899 teachers and school staff in Valparaíso, Chile. The results suggest that 22 items are suitable for use, showing good fit to the data. Smaller school size and school participation are associated with higher social well-being. The adapted scale can be a useful tool to measure socioaffective variables of adults in the school community. © 2025 Pontificia Universidad Catolica del Peru. All rights reservedhttps://revistas.pucp.edu.pe/index.php/psicologia/article/view/30102/2701
Signatures of the shock interaction as an additional power source in the nebular spectra of SN 2023ixf
No full textIndexación: Scopus.Red supergiants may lose significant mass during the final 100-1000 yr before core collapse, shaping their circumstellar environment. The superno va (SN) shockwav e propagating through this environment forms a shock-swept dense shell that interacts with the surrounding circumstellar material (CSM), generating secondary shocks that energize the ejecta and may power the SN during the nebular phase. In this work, we investigate the nebular spectrum of SN 2023ixf, observed 1-yr post- explosion (at + 363 d) with the recently commissioned WEAVE instrument on the 4.2 m William Herschel Telescope. This marks the first supernova spectrum captured with WEAVE. In this spectrum, H αexhibits a peculiar evolution, flanked by blueward and redward broad components centred at ∼±5650 km s −1 , features that have been observed in only a few SNe as early as 1-yr post-explosion. These features may indicate energy deposition from shock generated by the interaction of shock-swept dense shell with CSM expelled a few hundred years prior to the explosion. Comparisons of the + 363 d spectrum with model spectra from the literature suggest a shock power of at least ∼5 ×10 40 erg s −1 at this epoch. Additionally, analysis of the [O I ] doublet and other emission lines helped to constrain the oxygen mass (0 . 07 −0 . 30 M), He-core mass (3 M), and zero-age main sequence mass ( _ 12 M _) for SN 2023ixf. The comparison with other Type II SNe highlights SN 2023ixf's unique shock interaction signatures and evidence of dust formation, setting it apart in terms of evolution and dynamics. © 2025 The Author(s).https://www-scopus-com.recursosbiblioteca.unab.cl/pages/publications/86000585347S
Soccer players' physical performance adaptations with four weeks isometric versus dynamic contrast training: a randomized controlled trial
No full textIndexación: Web of Science.This randomized controlled study aimed to compare the effects of isometric contrast training (ICT) versus dynamic contrast training (DCT) on the physical performance of soccer players. Tier 3 male soccer players (age: 22.8 +/- 1.7 yrs) were randomly assigned to ICT (n=10), DCT (n=10), and control group (CG; n=10). Intervention groups performed similar lowload ballistic exercises, although the ICT and DCT incorporated push-isometrics or dynamic resistance high-load exercises, respectively. Aside from interventions, the off-season soccer training load was the same for control and intervention groups. Before and after 4-week of intervention, data were collected for 10 m, 20 m, and 40 m linear sprints, change of direction speed (CODS), countermovement jump (CMJ), standing long jump (SLJ), and 300-yard shuttle run test (SRT). After adjusting for baseline differences, statistical analysis revealed significant differences in all performance variables (p<0.001-0.018). Compared to controls, both intervention groups improved 40 m linear sprint, CODS, CMJ, and SRT (all p<0.05), and ICT also improved 10 m and 20 m linear sprint, and SLJ (all p<0.05). Relatedly, ICT improved 10 m linear sprint (p=0.001) and SLJ (p<0.001) when compared to DCT. In conclusion, soccer players improve physical performance with contrast training, particularly after ICT.https://www-webofscience-com.recursosbiblioteca.unab.cl/wos/woscc/full-record/WOS:00147980970000
Trichromatic color tuning strategy for emission of heterometallic EuIII/TbIII coordination polymers with triazolyl-substituted 4-methyl-phenoxo ligand
No full textIndexación: Scopus.This study presents the microwave-assisted synthesis and characterization of a series of heterometallic coordination polymers (HMCPs) with a 4-methyl-2,6-di[(1H-1,2,4-triazol-1-yl)]phenoxo ligand with varying EuIII/TbIII ratios. Single crystal X-ray diffraction reveals a double-chain structure bridged by triazolyl groups. Powder X-ray diffraction confirms the isostructural nature of the synthesized HMCPs. The photophysical properties depend on lanthanide ion concentration and excitation wavelength, leading to a color shift from green to blue as the proportion of TbIII decreases and EuIII increases. White light generation is achieved in the 8/2 EuIII/TbIII HMCP (CIE: 0.293, 0.326) under 335 nm excitation. The study suggests energy transfer from TbIII to EuIII, but both experimental and theoretical calculations indicate that this transfer is orders of magnitude lower than the sensitization through ligand states. © 2024 The Authorshttps://www.sciencedirect.com/science/article/pii/S1002072124002217?pes=vor&utm_source=scopus&getft_integrator=scopu
Baile chino: pilgrimage and building the nation in northern Chile
No full textIndexación: Revista UNAB.En este artículo analizamos la historia del baile chino de Iquique en función de dos elementos que nos parecen interesantes de destacar. El primero dice relación con el desplazamiento de este baile al Norte Grande y su posterior arraigo en la ciudad. El segundo, con que, en ese proceso, se han combinado muy bien dos elementos identitarios: el nacionalismo chileno y la identidad regional tarapaqueña. En este análisis, además explicitamos los vasos comunicantes que se produjeron entre las dos fiestas religiosas más grande del norte de Chile: Andacollo y La Tirana.In this research we analyze the History of The Iquique Chinese Dance in the role of two elements that we find interesting to stand out. The first one mentions the displacement of this dance to the North of Chile and its subsequent popularity in the city of Iquique. Immediately it is to empahasize that during this process two identity elements have well been combined: Chilean nationalism and the regional identity of Tarapaca. Throughout this analysis we are also interested in specifying the communicational links that have been produced between the two most important festivities in The North of Chile: Andacollo and La Tirana