31451 research outputs found
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In Vitro Cytotoxic and Apoptotic Effects of Boric Acid on Endometrial Adenocarcinoma Cell Lines (hec-1b and Ischikawa)
No full textEndometrial carcinoma, the most common malignancy of the female genital tract, remains challenging to treat despite early-stage dominance. Surgical interventions and irradiation are insufficient for advanced endometrial cancer. Our aim was to investigate to explore the in vitro cytotoxicity and apoptotic effects of boric acid (BA) on endometrial adenocarcinoma cell lines (Ishikawa and HEC-1B cell lines), providing experimental evidence for the potential application of boric acid as an anticancer drug. Time- and dose-dependent cell viability was determined with the XTT cell proliferation test. Differences in mRNA levels were determined by RT-PCR using cDNAs and SYBR green assay. Colony formation and the effect of BA on wound healing were evaluated. Immunocytochemistry and TUNEL tests were performed to evaluate apoptosis. BA increased the expression of Caspase 3 and Bax in HEC-1B and Ischikawa cell lines. It was determined that BA significantly decreased the number of colonies in both cell lines (p 0.05). In HEC-1B and Ishikawa cell lines, there was an increase in cell migration in the control group at 16 and 24 h. The apoptotic index was higher in the BA group, although it was not statistically significant. According to immunohistochemistry results, Caspase 3 and Bax expression in HEC-1B and Ishikawa cell lines were statistically increased in BA group. The expression of Bcl-2 was decreased statistically with BA treatment in both cell lines (p = 0.0001). BA treatment inhibited cell migration and colony formation, which are important for carcinogenesis, in endometrial adenocarcinoma cell lines. This inhibition was shown to occur through the apoptotic pathway.Scientific and Technological Research Council of Turkiye (TUBIdot;TAK); Pamukkale University Scientific Research Projects Coordination Unit [2023HZDP002]Open access funding provided by the Scientific and Technological Research Council of Turkiye (TUB ; Idot;TAK). This study was supported by The Pamukkale University Scientific Research Projects Coordination Unit (Grant 2023HZDP002)
Investigation of the Effect of Quercetin on the Accumulation of Lipids and the Level of Adiponectin in 3t3-L1 Adipocytes
No full textPurpose: Obesity disrupts the homeostasis of adipose tissue, leading to an increase in the number and size of adipose cells. Many positive effects of quercetin polyphenol on health through various mechanisms are known in the literature. The aim of our study was to investigate the effects of quercetin on lipid accumulation and adiponectin (ADP) levels in mature and hypertrophic adipocytes. Materials and methods: In this study, 3T3-L1 differentiated preadipocyte cells were exposed to high glucose media for 8 days to generate mature adipocytes, then for 18 days to produce hypertrophic adipocytes. Quercetin (40 and 80 µM) was administered to mature adipocytes for 24 hours and to hypertrophic adipocytes for 48 hours. Lipid content was visualized by Oil-Red-O staining method. Lipid accumulation and ADP level were measured by ELISA method. For statistical analysis, one-way analysis of variance (post hoc: Tukey test) was used. p≤0.05 was considered statistically significant. Results: The administration of both quercetin doses to mature adipocytes significantly reduced lipid accumulation (p=0.0001). The administration of 80 µM quercetin in mature adipocytes caused a significant increase in ADP levels (p=0.0001). Administration of quercetin to hypertrophic adipocytes caused a significant decrease in ADP levels (p=0.0001). Conclusion: Our study revealed that quercetin decreased lipid accumulation and increased ADP levels in mature adipocytes. However, in hypertrophic adipocytes, quercetin had no significant effect on lipid accumulation and decreased ADP levels. These findings suggest that quercetin has protective effects on health in the early stages of obesity, but its efficacy is limited in the later stages of obesity. © 2025, Pamukkale University. All rights reserved.PAU Scientific Research Projects Coordination Unit, (2023SABE009)This study was supported by Pamukkale University Scientific Research Projects Coordination Unit through Project number (2023SABE009)
Denizli İli İçin Yenilenebilir Enerji Kaynaklarının Çok Kriterli Karar Verme Yöntemleriyle Değerlendirilmesi
No full textIn this study, multi-criteria decision-making methods were applied to determine the priorities of renewable energy sources for Denizli. While most analyses about renewable energy sources for Turkey have focused on a national level or broader geographical areas, in this study, it is aimed to provide a detailed analysis for renewable energy potential for Denizli considering its geographical location, climate characteristics, and geological structure. Hybrid AHP-ARAS and SWARA-TOPSIS methods were used to evaluate and rank renewable energy sources based on economic, technical, environmental, and socio-political criteria. As a result, the most suitable renewable energy sources for Denizli were determined in the following order: solar, geothermal, wind, biomass, and hydroelectric energy. In addition, sensitivity analyses were carried out to assess risks in energy investments in the province. Hence, it is believed that the findings of this study have potential to contribute to decision-making and strategic planning processes for sustainable energy management in Denizli
The Effect of Healthcare Managers ' Perceptions of Top Management Team Behavioral Integration on Strategic Change and Innovative Performance
No full textIsik, Oguz/0000-0001-7368-7024Background: Healthcare organizations should constantly update their strategies, follow sectoral trends, provide continuous training and development opportunities to their employees, and create an environment conducive to innovation in order to adapt quickly to changes and gain competitive advantage. Purpose: The purpose of this study is to reveal the impact of senior managers' perceptions of the behavioral integration of the top management team on their perceptions of strategic change and innovative performance. Study design/methodology/approach: The study analyzed information obtained from senior managers of 321 private hospitals in Turkey. Multiple regression analysis was used to test the research hypotheses. Findings/conclusions: The results show that innovative performance is significantly enhanced by collaborative behavior, a sub-dimension of behavioral integration of the top management team. Moreover, another subdimension, joint decision making, has a negative effect on the initiation of strategic change, while collaborate behavior has a good effect on the execution of change. On the other hand, strategic change execution is negatively affected by joint decision making. The success of strategic change initiatives and the competitive advantage of the organization may be negatively affected. The influence of some cultural factors on business practices can create resistance to change and make it difficult for the top management team to work together. Overcoming this resistance requires cultural transformation and leadership development efforts within the organization. Limitations/future research: The study focuses only on the health sector. Further research can focus on other sectors with qualitative study methods
Ranking of Mitigation Measures in Decarbonisation of International Shipping of Turkish Ports: a Fuzzy-Analytic Hierarchy Process Approach
No full textDecarbonisation of shipping is very crucial to achieve the International Maritime Organization (IMO)'s zero-carbon target by 2035 especially in middle-income economies with relatively limited budget constraints. This paper purposes to demonstrate the importance of mitigation measures in decarbonisation of international shipping using a fuzzy- Analytic Hierarchy Process (AHP) approach. For this purpose, both main and sub-measures proposed by International Transport Forum in decarbonisation of international shipping are ranked in terms of their importance based on eight expert opinions working at an international port in T ; uuml;rkiye using survey data. The empirical evidence of the present paper reveals that alternative fuel and energy sources are the most important main mitigation measure of decarbonisation of international shipping. The results also put forward that more efficient use of ammonia is the most important sub-measure of decarbonisation of international shipping followed by light materials, slender design and solar energy use, respectively. More attention on the deployment of alternative fuel and energy sources including ammonia and solar energy is recommended for future decarbonisation policies of international shipping. The importance of combination of mitigation measures for decarbonisation of international shipping is also highlighted to achieve zero-carbon target by 2035
Anesthesia Videos in Geriatric and Elderly Patients on Youtube: Content, Quality, Reliability, and Usefulness Assessment
No full textIlhan, Seher/0000-0002-4857-8501Purpose. This study aimed to assess the quality, reliability, content, and usefulness of YouTube videos related to anesthesia in geriatric and elderly patients. Methods. Using Google Trends, the most popular search terms in the past five years, "geriatric anesthesia" and "anesthesia in the elderly," were identified. A search on YouTube using these terms initially yielded 200 of the most viewed videos for each term. After applying exclusion criteria, 87 videos were included for detailed analysis. Major themes and topics related to anesthesia in geriatric and elderly patients were identified using a pre-determined qualitative thematic analysis method. The usefulness of the videos was assessed using the specially developed Geriatric and Elderly Anesthesia Usefulness Score (GAEUS). The overall quality and reliability of the videos were evaluated using the Global Quality Scale (GQS) and the Modified DISCERN Scale (M-DISCERN), respectively. The average of the quality, reliability, and usefulness scores calculated by the researchers was used for consistency analysis. Results. In our study, 48.3% (42) of the videos on geriatric and elderly patients concerning anesthesia on YouTube were created by personal blogs. The quality of the videos was measured using the GQS, with a mean score of 3.34 and a median of 3 (range: 1-5), showing no significant difference according to the video source (p = 0.166). Reliability was assessed using the M-DISCERN scale, with a mean score of 3.37 and a median of 3.50 (range: 1-5), again showing no significant difference according to the video source (p = 0.097). Usefulness was measured using the GAEUS score, with a mean score of 15.30 and a median of 12.5 (range: 2-63), which showed a significant difference according to the video source (p = 0.000). The average duration of videos with low usefulness was 31.59 minutes (range: 5-44), while the average duration of moderately and highly useful videos was 59.37 minutes (range: 19.44-119.05). This duration difference was statistically significant (u = 2.569, p = 0.010). Conclusion. In our study, we examined YouTube videos covering anesthesia topics for geriatric and elderly patients. The highest usefulness scores were obtained from personal blogs; however, all sources generally showed low usefulness. The quality of the videos was assessed using the GQS, and their reliability was evaluated with the M-DISCERN. On both scales, the videos showed moderate performance across all sources. These findings indicate a need for more comprehensive and informative content on YouTube, especially for the education of healthcare professionals and patients. To better address the needs of elderly patients, the richness of content and educational value of these videos should be enhanced
A Detailed Study of the Combustion-Fuel Behavior of Nanofuels Containing Boron/Catalyst Nanohybrid Particles
No full textAgbulut, Umit/0000-0002-6635-6494Boron, with its high theoretical calorific value, is a promising alternative fuel, but its problematic oxidation behavior prevents complete combustion and limits its potential. This study focuses on the production of amorphous boron (AB) particles with reduced B2O3 layers using ball milling and their decorating with perovskite-type nano catalysts (La0.7 Nd0.3MnO3, Nd0. 7Ba0.3MnO3, La0.5 Nd0.3Ba0.2MnO3) by a cost-effective ultrasonication methods. The structural characterizations of all particles were characterized by SEM and XRD techniques. To evaluate the catalytic activity of the nanocatalysts, elemental analysis and surface area measurements were carried out by XPS and BET analysis, respectively. Combustion tests on gasoline-based nanofuels (2.5 and 7.5 wt %) in a controlled droplet-scale chamber showed that higher particle concentrations reduced ignition delay. However, boron hybrid particles had ignition delays similar to pure boron particles. Residual aggregate temperatures of 7.5 wt% AB-LNM1, AB-NBM, and AB-LNBM1 droplets were 111.5 %, 100 %, and 110.4 % higher than those with AB-BM. SEM-EDX analyses of residues revealed that AB-LNBM1 hybrids had the highest catalytic efficiency, with 5.47 % carbon and 17.53 % oxygen, significantly improving boron and soot oxidation. Engine tests using 250 ppm diesel blends highlighted NBM nanoparticles as having the lowest BSFC, while AB-LNBM1 achieved the highest HRR increase (12.69 %) and CO2 emissions (9.53 %) at 60 Nm. AB-LNBM1 also reduced HC emissions by 53.33 % at 15 Nm, and NBM provided the largest NOx reduction (9.70 %) at 30 Nm. Overall, boron/ catalyst nanohybrids enhanced combustion behavior, improved fuel efficiency, and reduced pollutant emissions. These findings suggest that such hybrids have significant potential for advancing alternative fuel applications and reducing the environmental impact of hydrocarbon fuels
Investigating the Effect of Coating and Synthesis Parameters on Lasub>1-x/Sub>srsub>x Based Core-Shell Magnetic Nanoparticles
No full textMagnetic nanoparticles are an important class of functional materials that have unique magnetic properties due to their reduced size (100 nm) and have the potential for use in many fields. In the preparation of magnetic nanoparticles, factors such as intrinsic magnetic properties, surface coating, size and shape of the particles, surface charge and stability are very important. In this regard, carefully determining the synthesis parameters of magnetic nanoparticles and particle coating materials is of critical importance in the application area chosen for the material. In this study, La1-xSrxMnO3 (x = 0.27, 0.30, 0.33) magnetic nanoparticles (MNPs), carbon-coated magnetic nanoparticles in core-shell structure (C@MNP) and their derivatives integrated into graphene oxide (GO-C@MNP) were synthesized and their properties were investigated in detail for their use in possible future application studies. The crystal structure of perovskite compounds with Pbnm symmetry remains unchanged after carbon coating but shrinks in volume due to its amorphous structure. The magnetic behavior of the uncoated and coated materials is almost identical, but the Curie temperature of the compounds shifts to a higher temperature. In the specific absorption ratio (SAR) measurements performed, it was found that the best SAR value for carbon-coated MNPs was 12.9 W/g at x = 0.27. By integrating the MNPs into graphene oxide, heat is easily distributed regionally, and this shows that the structures can be ideal candidates for applications such as hyperthermia, drug carriers, tissue repair, and cellular therapy including cell labeling and targeting. Perovskite-structured manganite materials were selected for their suitability in controlled production, where the Curie temperature can be tuned near the therapeutic temperature by adjusting the doping levels, making them ideal for magnetic hyperthermia applications. In this study, for the first time, the nanoparticle surfaces were coated with carbon, which was chosen not only due to carbon's non-magnetic nature but also because it provides an ideal platform for future combined biomedical applications such as drug delivery systems.Scientific and Technological Research Council of Turkey (TUBITAK) [221S429]; TUBITAKThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant Number 221S429. The authors thank TUBITAK for their support
The validity of Turkish version of Social Frailty Index in older adults: An index of social attributes predictive of mortality
No full textPurpose: The aim of this study was to examine the validity of Turkish version of Social Frailty Index in older adults. Material and methods: 102 older adults aged 65 and over were included in the study. Hodkinson Mental Test was first applied to older adults. Older adults who scored 6 points or above were included. To evaluate the validity of “Social Frailty Index”, Social Inclusion Scale, Older People's Quality of Life-Brief (OPQOL-Brief) and Lubben Social Network Scale were applied to the participants. To determine the reliability of “Social Frailty Index”, the test was re-tested at one-week intervals on older adults who did not receive any treatment. Confirmatory factor analysis AMOS 23.0 was used to determine the conformity of the structure to the original model. For concurrent validity, the correlation analysis applied. Intraclass correlation coefficient (ICC [2,1], absolute agreement, 2-way random effects model) was used to determine the reliability of the scale. Results: The fit of the Turkish version of Social Frailty Index to the model described in the original article was determined to be acceptable-excellent (Demographics (DEM), General Resources and Life History (GRLH), Social Activities (SA) and Fulfillment of Basic Social Needs (FBSN)) (CMIN/DF = 1.36, GFI = 0.93, IFI = 0.90, RMSEA = 0.06 and SRMR = 0.07). In the correlation analysis conducted to determine its concurrent validity, Social Frailty Index had a moderate correlation with Social Inclusion Scale (r: −0.47; p 0.001) and OPQOL-Brief (r: −0.47; p 0.001). Social Frailty Index had no correlation with Lubben Social Network Scale (r: −0.03; p: 0.81). Test–retest reliability of Social Frailty Index was excellent [ICC(2,1) = 0.90, %95 CI 0.84–0.93, p 0.001]. Conclusion: The Turkish version of the Social Frailty Index is valid and can be used to predict mortality risk based on the estimated social risk. The adapted Social Frailty Index is not adequately capturing aspects related to the network and social support. Clinical trials number: NCT06288789. © 2024 SEG
S-sulfocysteine's Toxic Effects on Ht-22 Cells Are Not Triggered by Glutamate Receptors, nor Do They Involve Apoptotic or Genotoxicity Mechanisms
No full textS-Sulfocysteine (SSC) is a metabolite derived from the metabolism of sulfur-containing amino acids. It has been implicated in neurotoxicity observed in children with sulfite oxidase deficiency. The aim of our study was to confirm the neurotoxic effects of SSC using a mouse hippocampal cell line (HT-22) and to investigate the role of apoptosis in these effects, especially in terms of caspase-3 activation and genotoxicity. Based on the viability graph obtained following increasing concentrations of SSC, we determined the LC50 dose of SSC to be 125 mu M by probit analysis. The cytotoxic effects of SSC were not reversed by glutamate receptor blocker administration. However, SSC treatment did not induce caspase-3 activation or induce DNA damage. Our results showed that SSC has a cytotoxic effect on neurons like glutamate, but glutamate receptor blockers reversed glutamate-induced toxicity, while these blockers did not protect neurons from SSC toxicity. The absence of caspase-3 activation and DNA fragmentation, which are indicative of apoptosis, in SSC-induced cell death suggests that alternative cell death pathways, such as necrosis and oxytosis may be implicated. Further research is necessary to fully elucidate SSC-induced cell death. The aim of our study was to confirm the neurotoxic effects of SSC using a mouse hippocampal cell line (HT-22) and to investigate the role of apoptosis in these effects, especially in terms of caspase-3 activation and genotoxicity.This study was a part of the PhD thesis of Volkan Tekin.Pamukkale niversites