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Stm1 Regulates Ifh1 Activity Revealing Crosstalk Between Ribosome Biogenesis and Ribosome Dormancy
No full textBianco, Eliana/0000-0003-1418-0527Nutrient abundance boosts ribosome biogenesis, whereas ribosome dormancy factors limit ribosome degradation upon starvation. The equilibrium between the two pathways governs cell growth. In this study, we identified suppressor of Tom1 (Stm1) as a molecular link between ribosome protection and biogenesis in Saccharomyces cerevisiae. While Stm1 was previously described as a dormancy factor, we show that it activates Ifh1, a transcriptional activator of ribosomal protein genes. Stm1 transiently localizes to the nucleolus, where it interacts with pre-ribosomes and directly binds RNA and Ifh1 through its C-terminal intrinsically disordered region (IDR). Although the IDR is dispensable for ribosome protection, its loss compromises cell growth. The IDR is phosphorylated upon nutrient starvation, which disrupts its interaction with Ifh1. Our findings reveal a molecular pathway sensing and adjusting ribosome abundance in response to nutrient availability, reinforcing the relevance of regulated ribosome homeostasis in physiology and disease.We thank Tom Dever, Jingwei Xu, Ludovic Gillet, Lennart Opitz, Marina Kunzi, and Anastasia Timofiva for their support with reagents, analysis and experiments; David Shore, Prashant Rawat, Caroline Wilson-Zbinden, and members of the Peter, Neurohr, and Weis laboratories for valuable scientific input; and the ETH ScopeM, Light Imaging Toulouse CBI, ETH FCGZ, ETH Flow Cytometry Core Facility, and the D-BIOL Biomolecular NMR Spectroscopy Platform for essential support. BioRender and Adobe Illustrator were used. M. Pilhofer is supported by the NOMIS Foundation, B.A. by the French National Research Agency (ANR-21-CE12-0008-01) , and the Peter laboratory by the Swiss National Science Foundation (SNSF) and ETH Zurich.NOMIS Foundation; French National Research Agency [ANR-21-CE12-0008-01]; Swiss National Science Foundation (SNSF); ETH Zuric
Beta kesirli türev içeren bazı matematiksel modellerin analitik çözümleri
No full textBu tez çalışması dört bölümden oluşmaktadır. Birinci bölümde kesirli
türev kavramı, gelişimi, kesirli türev ile ilişkili bazı özel fonksiyonlar, çeşitli
kesirli türev tanımları ve bu türev kavramı ile ilgili literatürde yer alan çalışmalara
değinilmiştir.
İkinci bölümde, Beta kesirli türev içeren bazı matematiksel modellerin
analitik çözümlerini bulmak için önemli rol oynayan homojen denge prensibi açık
bir şekilde ifade edilmiş ve Modifiye Kudryashov yöntemi ele alınmıştır.
Üçüncü bölüm tezin orijinal kısmını içermektedir. Dört farklı beta kesirli
mertebeden denklemden ilki Chafee-Infante ve diğerleri Geophysical KdV, Sığ su
dalgası, Gilson–Pickering denklemleridir. Burada denklemlerin tam çözümleri
Modifiye Kudryashov yöntemi kullanılarak elde edilmiş ve elde edilen
çözümlerin üç boyutlu grafiklerine her çözümün altında yer verilmiştir.
Dördüncü bölümde ise sonuç ve öneriler yer almaktadır.This thesis study consists of four chapters. In the first chapter, the concept
of fractional derivative, its development, some special functions related to
fractional derivative, various definitions of fractional derivative, and the studies in
the literature regarding this derivative concept are mentioned.
In the second part, the principle of homogeneous balance technique, which
plays an important role in finding analytical solutions of some mathematical
models containing Beta fractional derivatives, is clearly expressed, and the
Modified Kudryashov method is discussed.
The third part contains the original part of the thesis. The first of the four
different beta fractional order equations is Chafee-Infante and the others are
Geophysical KdV, shallow water wave, Gilson–Pickering equations. Here, the
exact solutions of the equations were obtained using the Modified Kudryashov
method, and three-dimensional graphs of the obtained solutions are included
under each solution.
In the fourth part, conclusions and recommendations are given
An Evaluation of the Adequacy of the Liberal Transfusion Strategy in Endoscopy-Assisted Metopic, Coronal, or Sagittal Craniosynostosis Surgeries: a Retrospective Observational Study
No full textEvran, Turan/0000-0003-4768-3622; Ilhan, Seher/0000-0002-4857-8501Background and Objectives: This study aims to evaluate the adequacy of the liberal transfusion strategy applied in patients undergoing endoscopy-assisted Metopic, Coronal, or Sagittal craniosynostosis surgery according to the Pre-Transfusion and Post-Transfusion Estimated Red Blood Cell Mass (ERCM) ratios. Materials and Methods: This retrospective cohort study, conducted at the Pamukkale University Faculty of Medicine (2017-2023), utilized anesthesia, surgical records, and hospital electronic data of patients undergoing endoscopic craniosynostosis surgery. The primary endpoints were the rates of Post-Transfusion 1st-hour ERCM/Pre-Transfusion ERCM (%) and Post-Transfusion 24th-hour ERCM/Pre-Transfusion ERCM (%). The secondary endpoints were determined as Hemoglobin (Hb) and Hematocrit (Hct) values at the 1st and 24th hours after surgery, Calculated Blood Loss (CBL) during surgery (%), total 24 h CBL (%), ERCM (%), and Estimated Blood Loss (EBV) during surgery and total 24 h transfusions, Packed Red Blood Cells (PRBCs) (mL/kg) amounts during surgery, and total 24 h transfusions. Results: A total of 86 pediatric craniosynostosis cases were evaluated and categorized into Metopic (n = 38), Sagittal (n = 33), and Coronal (n = 15) groups, with Post-Transfusion evaluation conducted across these groups. Post-Transfusion 1st-hour ERCM/Pre-Transfusion ERCM ratios were found to have median values of 90.70% in the Metopic group, 91.61% in the Sagittal group, and 93.09% in the Coronal group. Post-Transfusion 24th-hour ERCM/Pre-Transfusion ERCM ratios were found to be median values of 94.05% in the Metopic group, 88.3% in the Sagittal group, and 87.08% in the Coronal group. Conclusions: The liberal transfusion strategy provided adequate transfusion, maintaining ERCM ratios within the 85-115% range across all groups. Significant decreases in Hb and Hct levels were observed from preoperative to postoperative measurements at 1 and 24 h. Changes in CBL, ERCM, EBV, and PRBC volumes were noted between the postoperative 1 h and 24 h measurements across all groups
Plasma Levels of Adamts-13 Antigen, Adamts-13 Inhibitor, Adamts-13 Activity, and Von Willebrand Factor in Patients With Behcet's Disease
No full textBackground: Beh ; ccedil;et's Disease (BD) is characterised by recurrent aphthous oral and genital ulcers. Vascular involvement is one of the poor prognostic factors. Previously, von Willebrand Factor (VWF) has been detected higher in BD compared with healthy controls. We hypothesised that decreased activity or increased inhibitor levels of ADAMTS-13 may cause increased levels of VWF. Therefore, we investigated ADAMTS-13 in patients with BD. Methods: We included in total of 42 patients with BD and 41 healthy controls (HCs) in this cross-sectional study. Parametric data with normal distribution were compared with Student's t-test and ANOVA, and nonparametric data with non-normal distribution were compared with Mann Whitney U and Kruskal Wallis tests. Results: The patients showed lower ADAMTS-13 antigen, lower ADAMTS-13 inhibitor, lower ADAMTS-13 activity, and higher VWF levels compared with HCs. ADAMTS-13 activity was higher in vascular involvement compared with non-vascular involvement (18.26 +/- 7.3 vs 12.05 +/- 6.49, p=0.012). VWF levels were also similar between vascular and non-vascular subgroups. Conclusion: Reduced ADAMTS-13 activity and increased VWF levels were detected in BD. This change has not been seen in vascular BD. The underlying pathogenetic mechanisms seem more complex in the formation of thrombosis.This study was supported by the decision of the Pamukkale University Scientific Research Projects Coordination Unit dated 20.04.2019 and numbered 2019TIPF004.Pamukkale University Scientific Research Projects Coordination Unit [2019TIPF004
Two Unexplored Saponaria Species From Türkiye: Phenolic Composition and a Pharmacologic Potential
No full textOzay, Cennet/0000-0002-1120-6122; Aydin Kose, Fadime/0000-0001-5222-7555The total secondary metabolite amounts, phenolic profile, antioxidant activity, acute toxicity, cytotoxic, and wound-healing effects of the aerial parts of Saponaria kotschyi (endemic) and Saponaria pumilio extracts were investigated. The total phenolic, flavonoid, saponin, and tannin contents; antioxidant activity (via six different methods, including beta-carotene/ linoleic acid, DPPH, ABTS, FRAP, phosphomolybdenum, and metal chelating assays); phenolic constituents in the extracts (HPLC method); acute toxicity (brine shrimp lethality test); antiproliferative effects on human cervical carcinoma (HeLa) and human neuroblastoma (SH-SY5Y) cancer cell lines (MTT assay); and the effects on wound healing in healthy mouse fibroblast cells (NIH-3T3) through an in vitro scratch assay were evaluated. The methanol extract of S. kotschyi demonstrated higher concentrations of secondary metabolites and antioxidant activity than S. pumilio. Both species predominantly contained caffeic acid, 2,5-dihydroxybenzoic acid, and epicatechin, though S. pumilio had a notably higher caffeic acid content. Additionally, S. kotschyi extract showed greater cytotoxicity against HeLa and SH-SY5Y cancer cells, while S. pumilio exhibited greater wound-healing efficacy. Two previously unexplored Saponaria species reveal a remarkable richness in secondary metabolites and potent antioxidant activity. Given their diverse phenolic constituents, these species emerge as promising candidates for therapeutic applications in cervical cancer and neuroblastoma, as well as playing a potentially critical role in tissue repair and regeneration-areas that merit further in-depth investigation.Scientific Research Projects Coordination Unit, Pamukkale University, Turkiye [2018FEBE043]This study was financially supported by the Scientific Research Projects Coordination Unit (Project no: 2018FEBE043) , Pamukkale University, Turkiye
Effects of Motivational Interviewing and an Education Programme Based on Watson's Theory of Human Caring on Adults Receiving Haemodialysis: Randomised Controlled Trial
No full textYangoz, Sefika Tugba/0000-0001-9711-2620Background: Effective haemodialysis management is crucial, and behavioural change interventions are essential for good haemodialysis self-management. However, no study explores the effects of Watson's theory such as the humanistic approach to behavioural change in haemodialysis.Aims: To evaluate the effects of motivational interviewing and an education programme based on Watson's theory on adherence to treatment, satisfaction and health-related quality of life (HRQOL) on haemodialysis.Methods: This study was conducted in Turkey. Intervention group received four sessions of motivational interviewing and an education programme based on Watson's theory, and the control group received routine care. The primary outcomes were adherence to fluid intake, diet and medication, attendance at haemodialysis sessions, satisfaction and HRQOL. The secondary outcomes were laboratory parameters.Results: Participants were randomly assigned to the intervention (n = 32) or the control group (n = 28). There were statistically significant between-group differences in adherence to fluid intake, diet and medication, and the kidney disease burden sub-dimension of HRQOL, with medium to large effect sizes. There was no difference in laboratory parameters. Participants showed high levels of satisfaction with this programme.Conclusions: This study provides evidence for nurses regarding Watson's theory-based programme in a haemodialysis population. Results will help nurses to develop and practice customised effective interventions for haemodialysis.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was funded by the Scientific Research Projects Coordination Unit with the Project number 'TDK-2018-3279' at Akdeniz University in Turkey.Akdeniz University in Turkey [TDK-2018-3279
A study of the great mother archetype in an industrially transformed world in selected stories of Sarah OrneJewett, sherwood anderson, and Eudora Welty
No full textAccording to Carl Gustav Jung’s theory, the collective unconscious is not based on individual experience, but every person’s experience is stored side by side, which each person acquires through genetics as well as being recalled by individuals when necessary. People share the same morals, ethics, and sense of justice regardless of their culture. The unconscious contains the earliest shapes, symbols, and images in relation to the shared values. He uses human imagery and symbols to understand the collective unconscious, and these are called archetypes, which may surface in dreams, art, and literature. One of these beginning imprints is The Great Mother archetype suggested and developed by Erich Neumann following Carl Gustav Jung’s theory of the collective unconscious. Human minds established a parallel between the earth’s life-giving qualities and the female body, attributing both with sacredness. Furthermore, this sacred female figure owns different faces which contribute to the stages in a woman’s life. This study aims to analyse selected stories of three chronologically consecutive authors, Sarah Orne Jewett, Sherwood Anderson, and Eudora Welty, in terms of changing society/order under the influence of industrialisation and urbanisation and how this change is reflected through the mother archetype. In the stories of these three authors, the mother archetype will be examined in two dimensions: as Mother Nature and as the human representatives of the Great Mother. Female characters from different generations and their connections to the archetype will therefore help illuminate this transformation.Carl Gustav Jung'un teorisine göre, kolektif bilinçdışı bireysel deneyimlere dayanmaz, ancak her insanın deneyimi yan yana depolanır ve her insan genetik olarak edindiği gibi gerektiğinde bireyler tarafından da geri çağrılır. İnsanlar kültürlerinden bağımsız olarak aynı ahlak, etik ve adalet duygusunu paylaşırlar. Bilinçdışı, paylaşılan değerlerle ilgili en eski şekilleri, sembolleri ve imgeleri içerir. Kolektif bilinçdışını anlamak için insan imgelerini ve sembollerini kullanır ve bunlar rüyalarda, sanatta ve edebiyatta ortaya çıkabilen arketipler olarak adlandırılır. Bu başlangıç izlerinden biri, Carl Gustav Jung'un kolektif bilinçdışı teorisini takiben Erich Neumann tarafından önerilen ve geliştirilen Büyük Anne arketipidir. İnsan zihni, dünyanın hayat veren nitelikleri ile kadın bedeni arasında bir paralellik kurarak her ikisine de kutsallık atfetmiştir. Dahası, bu kutsal kadın figürü, bir kadının hayatındaki aşamalara katkıda bulunan farklı yüzlere sahiptir. Bu çalışma, kronolojik olarak birbirini takip eden üç yazarın, Sarah Orne Jewett, Sherwood Anderson ve Eudora Welty'nin seçilmiş öykülerini, sanayileşme ve kentleşmenin etkisiyle değişen toplum/düzen ve bu değişimin anne arketipi üzerinden nasıl yansıtıldığı açısından incelemeyi amaçlamaktadır. Bu üç yazarın öykülerinde anne arketipi iki boyutta incelenecektir: Doğa Ana ve Büyük Anne'nin insan temsilcileri olarak. Dolayısıyla, farklı kuşaklardan kadın karakterler ve onların arketiple olan bağlantıları bu dönüşümü aydınlatmaya yardımcı olacaktır
Emotional and Psychological Well-Being, Emotion Regulation, and Intent Attribution of Children Whose Earthquake Bereaved
No full textErol, Ahmet/0000-0002-7538-952XThis study compares the emotion regulation, intent attribution, and emotional and psychological well-being (PWB) of children who lost their loved ones in the earthquakes that occurred in T ; uuml;rkiye on February 6, 2023, with children who were not directly affected by the earthquakes and who were nonbereaved. The participants consisted of 216 children living in earthquake zones who were bereaved and 202 children who were not living in earthquake zones and were nonbereaved. As a result of the study, it was found that the PWB and emotion regulation of children who were nonbereaved were statistically significantly higher than those of children who were bereaved. No significant difference was found between bereaved and nonbereaved children in terms of intent attribution. In addition, it was determined that significant positive relationships existed between PWB and emotion regulation in children who were nonbereaved. These relationships were seen at a lower level in children who were bereaved
Forecasting Wind Farm Production in the Short, Medium, and Long Terms Using Various Machine Learning Algorithms
No full textWind energy is a crucial renewable resource for sustainable power generation; however, challenges such as high initial investment costs and difficulties in identifying efficient locations hinder its widespread adoption. Accurate wind energy forecasting is essential for energy planning, trading, and grid optimization. This study presents short-term, medium-term, and long-term -wind power forecasts for the S ; ouml;ke-; Ccedil;atalb ; uuml;k Wind Power Plant in Ayd ; imath;n, Turkey, using meteorological data and production records from 2018 to 2022. Five machine learning algorithms were employed-Artificial Neural Network (ANN), Random Forest (RF), Extreme Gradient Boosting (XGBoost), K-Nearest Neighbors Regression (KNN), and Multi-Layer Perceptron (MLP ANN)-utilizing both MinMax and Standard Scaling methods. Prediction performance was evaluated using Mean Absolute Error (MAE), Coefficient of Determination (R2), and Root Mean Square Error (RMSE) metrics. The results indicate that Min-Max Scaling improved short-term predictions with KNN, while XGBoost and Random Forest provided more stable and accurate forecasts in medium- and long-term predictions. Additionally, Standard Scaling significantly enhanced MLP ANN's performance in medium-term forecasting. These findings provide practical insights for optimizing wind energy forecasting models, which can improve energy trading strategies, enhance grid stability, and support informed decision making in renewable energy investments. The results are particularly valuable for energy planners and policymakers seeking to maximize the efficiency of wind power plants and facilitate the integration of renewable energy sources into national grids more effectively
Evaluation of the Mid-Term Surgical Outcomes of Vnotes Sacrocolpopexy and Vnotes High Uterosacral Ligament Suspension in Pelvic Organ Prolapse
No full textBackgroundThis multicenter retrospective study aimed to assess the surgical outcomes of vNOTES-HUSLS and sacrocolpopexy after vNOTES hysterectomy for apical pelvic organ prolapse (POP).MethodsA total of 101 patients were included. Pre and postoperative modified POP-Q scores, postoperative Visual Analog Scale (VAS) scores and complications were among the information gathered. POP-Q-C score of less than -1 cm was considered an apical recurrence. 'Overall success' was defined as the absence of each of the following factors: >= Stage 2 POP (in any compartment), retreatment for prolapse.ResultsvNOTES-HUSLS (n = 78) achieved an overall success rate of 92.2%, accompanied by a low intraoperative complication rate of 2.6% (n = 2) at the 23-month median follow-up. vNOTES-sacrocolpopexy (n = 23) achieved an anatomical success rate of 78.3% and exhibited a low intraoperative complication rate of 4.3% (n = 1) at the 21-month median follow-up. The apical prolapse recurrence rate was 2.6% and 8.7% in the HUSLS and sacrocolpopexy groups, respectively (p = 0.185). Total duration of surgery and VAS scores were significantly lower in the vNOTES-HUSLS group than in the vNOTES-sacrocolpopexy group.ConclusionsIn POP surgery, HUSLS and sacrocolpopexy may be performed using the vNOTES technique depending on the patient's preference for natural tissue repair or mesh use