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Beyond Pigment Synthesis: Investigating Dopachrome Tautomerase (DCT) Localization and Interactions with Cell Cycle Proteins
No full textMelanoma is a highly lethal skin cancer with limited therapeutic options once it metastasizes, largely due to acquired resistance to immunotherapy and targeted treatments. Dopachrome tautomerase (DCT), formerly recognized for its enzymatic contribution to pigment synthesis, is now emerging as a biomarker and molecular driver associated with poor prognosis, stress resistance, and therapy failure in melanoma. Although pigment production is not strictly required for melanocyte survival, evidence reveals that DCT’s retained and upregulated expression in melanoma supports tumor resilience and adaptation under therapeutic stress.
This study addresses how DCT contributes to melanoma cell proliferation and cell cycle regulation through non-canonical mechanisms. Using immunofluorescence assays, cell cycle synchronization, RNAi-mediated knockdown, proximity labeling, and lentiviral transduction, we examine DCT’s spatial dynamics and molecular interactions. DCT localization to the nuclear envelope is dependent on cell cycle stage, and proximity labeling assays reveal DCT’s selective interaction with Cyclin-dependent kinase 2 (CDK2), a cell cycle kinase crucial for melanoma survival, specifically in melanoma cell lines. Functional studies indicate that knockdown of DCT causes G1/S cell cycle blockade and suppresses clonal expansion and tumor growth, both in culture and animal models. Furthermore, proximity ligation confirms endogenous nuclear co-localization of DCT and CDK2, implicating DCT’s nuclear interactions in cell cycle control.
Key findings show that DCT, beyond its classical role in melanogenesis, localizes to the nucleus in melanoma cells, particularly in the contexts where CDK2 is also nuclear. Although DCT and CDK2 co-localize in the nucleus, and this may support melanoma cell proliferation and cell cycle regulation, we do not directly demonstrate effects on therapy resistance in this study. Future research should focus on elucidating the functional relationship between DCT nuclear localization, CDK2 activation, and cell cycle control to determine whether DCT-mediated pathways contribute to stress responses or resistance phenotypes in advanced melanoma.A three-year embargo was granted for this item.Academic Major: Molecular Genetic
Contemporary Feminist Fiction and a Case for Expanding Rhetorical Narratology
No full textItem embargoed for five year
Publications and Services of Library Publishing Programs in the United States and Canada Dataset
No full textThis dataset is the transformed data of a subset of the public dataset issued by the Library Publishing Coalition that can be found here: https://drive.google.com/uc?export=download&id=1Z1lvz3BaXUi1Lc_QYbXRKTLIsyTCKOcM . It contains information about the publications and services of library publishing programs gathered by the Library Publishing Coalition's annual Directory Survey, and also adds enrollment information for the institutions included.Publisher allows immediate open acces
Plan-and-practice: Examples of student submissions
No full textThis is a supplementary file for an article published in Engaging Students: https://doi.org/10.18061/esm.698
Interview of Randy Rydell by Cory Ackerman
No full textThis oral history interview with Randy Rydell explores his career in nuclear nonproliferation and his tenure working for Senator Glenn as a member of the professional staff on the Senate Committee on Governmental Affairs.
Rydell recounts formative childhood experiences abroad that inspired his commitment to international policy, leading to advanced studies in international relations and political science. His expertise in nuclear security, particularly breeder reactors and plutonium policy, aligned with Senator Glenn’s legislative priorities. Rydell discusses Glenn’s leadership on the Nuclear Non-Proliferation Act of 1978 and the Nuclear Proliferation Prevention Act of 1994, emphasizing Glenn’s advocacy for strict export controls and global disarmament norms. The interview highlights Glenn’s bipartisan approach, integrity, and respect for staff, as well as his ability to communicate complex issues with clarity and conviction. Rydell reflects on navigating the complex diplomatic and policy challenges involving Japan, China, and Pakistan, and the challenges of balancing national security with geopolitical interests.
Personal anecdotes illustrate Glenn’s humanity, humor, and dedication to public service. Rydell also shares his transition to the United Nations and continued commitment to Glenn’s legacy through support for the John Glenn College of Public Affairs. The interview offers a rich portrait of Glenn’s impact on nuclear policy and the enduring value of principled public service.This oral history was conducted in partnership with History Associates, Inc. and the John Glenn College of Public Affairs at the Ohio State University
Harnessing the Silver Linings of COVID-19: Using Remote Work to Accommodate an Employee's Commute Under the ADA
Get PDFTruth Be Told: White Nostalgia and Antiracist Queer Resistance in "Post-Truth" America
No full textBlack Lives Matter, violent imaginaries, and narrative activism -- Activist relational knowledge against digital disinformation in Black Lives Matter and the Women's March -- Charlottesville's false equivalencies : rejecting "both sides" by refusing to waste time -- Whose insurrection? Rhetorical gaslighting after January 6 and the uses and limits of testimonial acts -- Anti-trans disinformation and t4t care through joy and spiteItem embargoed for three year
The Greeks of Mestá, Chios
Get PDFMr. John Kellis of Steubenville, Ohio, a nonagenarian who is the last remaining speaker of the old village dialect of the village of Μεστά (Mestá) on the island of Chios, has devoted years of his life to producing a three-volume work that documents the dialect, customs, and history of the village, by way of offering a window into the past of Mestá and preserving aspects of life in the village in times gone by.Mestá -- People of Mestá -- Tales of Mest
