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Greener Synthesis of Sulfosuccinimidyl 6-((4-azido-2-nitrophenyl) amino) hexanoate
Get PDFThe current manufacturing process for Sulfosuccinimidyl 6-((4-azido-2-nitrophenyl) amino)hexanoate (Sulfo-SANPAH) involves an organic solvent system containing 60% acetone to synthesize 6-((4-azido-2-nitrophenyl) amino)hexanoic (AMPAH) acid, the precursor to the final structure. Though acetone is relatively safe, there is a potential method to mitigate flammability risks during production by using only water as the solvent to synthesize AMPAH acid. This hetero-bifunctional crosslinker, essential for cell-surface protein crosslinking, contains dual reactive groups: a photoreactive nitrophenyl azide and an amine-reactive Sulfo N-hydroxy succinimide (NHS) ester. The molecular design preserves both the nitrene group formation under UV exposure for C-H, N-H, and ring expansion insertions, and the NHS ester reactivity with primary amines at pH 7-9 for stable amide bond formation. The method of synthesizing the precursor to Sulfo-SANPAH demonstrated equivalent sulfonation efficiency to the original material while significantly reducing process safety risks. This improved synthetic approach provides a safer manufacturing route for this important biological conjugation reagent.No embargoAcademic Major: Pharmaceutical Science
The Ninth Annual Humanism in Medicine Last Lecture
Get PDF2025 Humanism in Medicine Last Lecture, "The Heart of the Pediatrician: Helping Children Feel Lovable, Capable and Valuable", featuring Mary McIlroy, MD, Emerita Faculty, Department of Pediatrics, The Ohio State University College of Medicine, August 28, 2025, The Ohio State University Richard M. Ross Heart Hospital Auditorium, Columbus, Ohio.Dr. Mary McIlroy is the ninth Last Lecture speaker. She graduated from The Ohio State University College of Medicine and completed her pediatric residency at Nationwide Children's Hospital, including an additional year as Chief Resident. She then devoted 36 years to the students, residents, and faculty at the Ohio State College of Medicine and the Department of Pediatrics and Nationwide Children's Hospital.
Dr. McIlroy's passion for education led to her leadership and teaching of multiple aspects of the college curriculum, including teaching pediatric topics and mentoring students, developing and managing the clinical curriculum, and assessing and evaluating student progress. She directed the Med 3-4 Academic Program and the Pediatric Clerkship for decades, served as co-chair of the college General Objectives Task Force, and chaired the Executive Curriculum Committee, along with multiple other positions. At Nationwide Children's she was also Assistant Medical Director of Education and chair of the Graduate Medical Education Committee.
Dr. McIlroy counseled and guided many medical students, particularly those interested in pursuing pediatric careers, through a period of years when the college numbers far exceeded the national average of the percentage of students from medical schools entering pediatric residencies. She treasures the letters of gratitude and updates she received from students over many years and the continuing personal connections with them.
Dr. McIlroy has been honored with the College of Medicine Distinguished Educator Award, the Faculty Teaching Award and Master Teacher designation, the Gold Humanism Honor Society Mentoring Award, the College of Medicine Alumni Achievement Award, the College of Medicine Lifetime Achievement Award, and the Nationwide Children's Hospital Career Contribution Award.Courage to Teach BoardJames V. Warren, MD, Medical Humanities FundThe Linda C. Stone, MD, Program for Humanism and the Arts in MedicineThe Ohio State University College of Medicin
Moderating Role of Poor Vascular Health in Dementia and Depression
No full textDepression and dementia commonly co-occur in older adults; yet the mechanisms linking them remain unclear. Given that vascular risk contributes to both depression and dementia, it may serve as a key moderator in their association. In this study, it was hypothesized that vascular risk would moderate the relationship between depression and dementia. It was also hypothesized that increased depression scores would be positively correlated with vascular risk factors, individuals with higher vascular risk would perform worse on cognitive assessments, and depression and memory impairment would be positively correlated. Baseline data were drawn from a large NIMH- funded clinical trial with 108 participants evaluating the efficacy of anxiety treatment in reducing anxiety and related symptoms among those with MCI or mild ADRD. Dementia was measured through objective (RBANS) and subjective (MCS and QDRS) measures. Biological measures for dementia were reflected in grip strength. Other measures included the MINI, PROMIS, and vascular risk count. Among participants, 71.8% were female and 28.2% were male. Participants identified as 7.7% Black/African American, 73.8% Caucasian, and 4.1% multiracial. Ethnically, participants identified as 2.1% Hispanic or Latino, 94.9% not Hispanic or Latino, and 3.1% unknown. Results indicated that depression predicted functional outcomes (MCS, QDRS), but not objective; vascular risk predicted immediate memory scores; and vascular risk moderated language and immediate memory. These findings suggests that vascular risk worsens specific memory domains and depression could predict functional outcomes.No embargoAcademic Major: Neuroscienc
Sacred Sisterhoods: A Celebration of Black Women's Friendships on Television and in Film
No full textItem embargoed for three year
Nordic Myths Exhibition at Nationalmuseum Jamtli in Östersund, Sweden, Part I: Nature, Medievalism, and The Construction of Space
No full textProhexadione-calcium Foliar Sprays for Growth Regulation in Herbaceous Perennial Ornamental Grass Taxa
Get PDFExcessive vegetative growth of ornamental grasses presents several production challenges for commercial greenhouse and nursery growers. Prohexadione-calcium, a plant growth regulator (PGR), has been used to reduce growth in a variety of fruit and grain crops. The foliar influence of prohexadione-calcium on five popular and commercially available ornamental grasses was evaluated in this study. Calamagrostis acutiflora ‘Karl Foerster’, Miscanthus sinensis ‘Huron Sunrise’, Panicum virgatum ‘Red Flame’, Pennisetum alopecuroides ‘Hameln’, and Schizachyrium scoparium ‘Twilight Zone’ were sprayed with 0, 500, 1000, 1500, 2000, 2500, 3000, or 5000 mg·L–1 prohexadione-calcium in a completely randomized block design. Each species received one spray application and were evaluated 2, 4, and 6 weeks after application to determine plant height and diameter. After 6 weeks, the experiment was terminated, and plants were excised at the substrate level to determine shoot dry weight. Prohexadione-calcium
significantly (P ≤ 0.05) reduced plant height, plant diameter, and shoot dry weight of Miscanthus ‘Huron Sunrise’, compared to the control. Plant height and plant diameter of Schizachyrium ‘Twilight Zone’ was only affected 6 weeks after application and reduced shoot dry weight. While prohexadione-calcium applications on Miscanthus ‘Huron Sunrise’, Panicum ‘Red Flame’ and Pennisetum ‘Hameln’ had minimal to no effect on plant height or plant diameter but varying significance on shoot dry weight. Data from this study suggests that foliar application of prohexadione-calcium on ornamental grasses shows promise but is species- and concentration specific.The Ohio State Floriculture and Landscape Horticulture Alumni Association Fellowship Endowment FundFine Americas, IncSun Gro HorticultureJ.R. Peters, Inc.Walters Gardens, Inc.No embargoAcademic Major: Sustainable Plant System
Ordinary Queerness in American Modernism
No full textIntroduction: An acquaintance with the ordinary -- The sex lives of spinsters: Sarah Orne Jewett's distant intimacies -- "She would polish and hone that": Gwendolyn Brooks and the pleasures of form -- "They were regularly gay there": Gertrude Stein's explicit abstraction -- "'Rose rocks' or maybe 'rock roses'": Elizabeth Bishop's "passion for accuracy" -- Epilogue: Ordinary readingItem embargoed for five year
NEUROTENSIN MODULATION OF ULTRASONIC VOCALIZATION USING SYSTEMIC NEONATAL SEPSIS MODEL
Get PDFNeonatal sepsis is the leading cause of morbidity and mortality in newborns. The presence of non-specific signs and symptoms caused by invading pathogens hinders early recognition and diagnosis in neonatal sepsis. The brainstem neural circuits that maintain automatic breathing become disrupted in response to sepsis-induced neuroinflammation, leading to respiratory dysfunction. In addition, breathing circuits interact with eliciting vocalizations during the crying phase in newborns, showing a fundamental coordination of both circuits. Therapeutic hypothermia is defined as a 2-6°C reduction in body temperature that can act as neuroprotective for sepsis-induced encephalopathy. Neurotensin is a 13 amino acid peptide that binds to NTSR1 and NTSR2, promoting hypothermia in rodents. A poorly understood interaction exists between therapeutic hypothermia, neurotensin, and inflammation for breathing-vocalization circuits. Our hypothesis states that therapeutic hypothermia could provide neuroprotection and ultimately preserve the brainstem functions, including the respiratory-vocalization circuit integrity. This thesis investigated the potential of therapeutical hypothermia using neurotensin agonist (PD149163) to modulate respiratory circuit integrity following sepsis.
We utilized a preclinical experimental model utilizing TLR ligands such as LPS (TLR4 agonist) and PAM3CSK4 (TLR1/2 agonist) to mimic systemic inflammatory response promoted by gram-negative and gram-positive bacterial infection. Postnatal day 5 CD1 mouse pups were exposed to either saline or neurotensin pretreatment, followed by sepsis induction. Physiological and behavioral assessments were performed 3 hours following the TLR ligands administration. We leveraged ultrasonic vocalizations (USVs) and whole-body plethysmography (WBP) to assess respiratory circuit integrity. USVs and WBP were used as noninvasive approaches to provide a readout of intact respiratory-vocalization circuits in rodent models. Data analysis utilized machine-learning pipelines including MUPET and VocalMat in MATLAB for USVs recordings. R programming language and environment were utilized for statistical computing and graphics.
Neurotensin agonist was effective inducing hypothermia in PD5 control pups. As previously reported by our lab, LPS also induced hypothermic effects. PD149163 paired with LPS further reduced the body temperature whereas PAM3CSK4 or paired with PD149163 did not promote changes. Maternal retrieval time increased in response to LPS, and it was aggravated by PD149163. USV complexity was deeply affected by neonatal sepsis changes due to LPS, reducing total calls, syllable variety, and repertoire units. PD149163 further exacerbated these vocal deficits with LPS-induced sepsis but partially restored complex call types in the PAM3CSK4 group. The WBP assessed the respiratory changes due to sepsis and hypothermia. The minute ventilation was depressed in baseline recordings of LPS and LPS + PD149163 groups. Hypercapnia was used to evoke a central ventilatory response; sepsis results in a depressed minute ventilation response which was further amplified with PD149163. The hypothermic effects due to PD149163 resulted in depressed minute ventilation during hypercapnia. Together, these findings demonstrate that neurotensin modulates brainstem respiratory-vocalization circuitry which might not be beneficial upon gram-negative bacterial induced inflammation.
This work provides new insight into how thermoregulation, immune activation, and neural control of breathing and communication interact in early life. By revealing the conditions under which neurotensin signaling is protective or detrimental, this thesis establishes a foundation for future studies exploring neurotensin modulation as a potential regulator of neonatal responses during infection.No embargoAcademic Major: Microbiolog
