13197 research outputs found
Sort by
An Empirical Study on the First Reponses to First-Time Contributors in OSS Projects
Get PDF奈良先端科学技術大学院大学修士 (工学)master thesi
Elucidation of the nutrient-dependent regulatory mechanisms that control the ammonium transporter gene amt1+ in fission yeast
Get PDF奈良先端科学技術大学院大学博士(バイオサイエンス)doctoral thesi
An Exploration on the Emotionality in Online Fan Communities of Different Music Genres
Get PDF奈良先端科学技術大学院大学修士(工学)master thesi
フトクテイ タスウ ノ ヒト ガ アノテーション ニ サンカデキル ツール ダレカノ オヨビ Comment Share ノ カイハツ ト セイノウ ヒョウカ
Get PDF奈良先端科学技術大学院大学修士(工学)master thesi
Multidimensional analysis of extracellular potentials in the cerebral cortex
No full textvideo/mp4When a microelectrodes is inserted into the cerebral cortex, extracellular potentials reflecting neuronal firing and local field potentials (LFPs) can be recorded. How to record them, how to analyze them, and what results can be obtained will be discussed based on my own research. In particular, I will show how multidimensional information can be obtained from a single electrical signal. The importance of neurophysiology using monkeys will also be introduced.講演日: 2022年6月1日 3限講演場所:エーアイ大講義室vide
Two Kinesin-14A Motors Oligomerize to Drive Poleward Microtubule Convergence for Acentrosomal Spindle Morphogenesis in Arabidopsis thaliana
No full textPlant cells form acentrosomal spindles with microtubules (MTs) converged toward two structurally undefined poles by employing MT minus end-directed Kinesin-14 motors. To date, it is unclear whether the convergent bipolar MT array assumes unified poles in plant spindles, and if so, how such a goal is achieved. Among six classes of Kinesin-14 motors in Arabidopsis thaliana, the Kinesin-14A motors ATK1 (KatA) and ATK5 share the essential function in spindle morphogenesis. To understand how the two functionally redundant Kinesin-14A motors contributed to the spindle assembly, we had ATK1-GFP and ATK5-GFP fusion proteins expressed in their corresponding null mutants and found that they were functionally comparable to their native forms. Although ATK1 was a nuclear protein and ATK5 cytoplasmic prior to nuclear envelop breakdown, at later mitotic stages, the two motors shared similar localization patterns of uniform association with both spindle and phragmoplast MTs. We found that ATK1 and ATK5 were rapidly concentrated toward unified polar foci when cells were under hyperosmotic conditions. Concomitantly, spindle poles became perfectly focused as if there were centrosome-like MT-organizing centers where ATK1 and ATK5 were highly enriched and at which kinetochore fibers pointed. The separation of ATK1/ATK5-highlighted MTs from those of kinetochore fibers suggested that the motors translocated interpolar MTs. Our protein purification and live-cell imaging results showed that ATK1 and ATK5 are associated with each other in vivo. The stress-induced spindle pole convergence was also accompanied by poleward accumulation of the MT nucleator γ-tubulin. These results led to the conclusion that the two Kinesin-14A motors formed oligomeric motor complexes that drove MT translocation toward the spindle pole to establish acentrosomal spindles with convergent poles.journal articl
KIF24 depletion induces clustering of supernumerary centrosomes in PDAC cells
No full textClustering of supernumerary centrosomes, which potentially leads to cell survival and chromosomal instability, is frequently observed in cancers. However, the molecular mechanisms that control centrosome clustering remain largely unknown. The centrosomal kinesin KIF24 was previously shown to restrain the assembly of primary cilia in mammalian cells. Here, we revealed that KIF24 depletion suppresses multipolar spindle formation by clustering centrosomes in pancreatic ductal adenocarcinoma (PDAC) cells harboring supernumerary centrosomes. KIF24 depletion also induced hyper-proliferation and improved mitotic progression in PDAC cells. In contrast, disruption of primary cilia failed to affect the proliferation and spindle formation in KIF24-depleted cells. These results suggest a novel role for KIF24 in suppressing centrosome clustering independent of primary ciliation in centrosome-amplified PDAC cells.journal articl
Strigolactones are chemoattractants for host tropism in Orobanchaceae parasitic plants
No full textParasitic plants are worldwide threats that damage major agricultural crops. To initiate infection, parasitic plants have developed the ability to locate hosts and grow towards them. This ability, called host tropism, is critical for parasite survival, but its underlying mechanism remains mostly unresolved. To characterise host tropism, we used the model facultative root parasite Phtheirospermum japonicum, a member of the Orobanchaceae. Here, we show that strigolactones (SLs) function as host-derived chemoattractants. Chemotropism to SLs is also found in Striga hermonthica, a parasitic member of the Orobanchaceae, but not in non-parasites. Intriguingly, chemotropism to SLs in P. japonicum is attenuated in ammonium ion-rich conditions, where SLs are perceived, but the resulting asymmetrical accumulation of the auxin transporter PIN2 is diminished. P. japonicum encodes putative receptors that sense exogenous SLs, whereas expression of a dominant-negative form reduces its chemotropic ability. We propose a function for SLs as navigators for parasite roots.journal articl
Comprehensive and engineering studies of the yeast branched-chain amino acid aminotransferases for improvement of fusel alcohol production
Get PDF奈良先端科学技術大学院大学博士(バイオサイエンス)doctoral thesi