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Equol-Producing Bacteria in the Chicken Intestine: PCR Analysis on the Bacteria Quantitatively Correlating With Equol Concentration in Daidzein-Fed Laying Hens
No full textEquol is a microbial metabolite from daidzein, an isoflavone, in the animal intestine. This study aimed to identify equol-producing bacteria in the intestines of chickens. Sixteen 181-day-old Hy-Line Brown laying hens were given a basal diet formulated excluding soy products for 2 weeks and subsequently given the diets supplemented with 0-, 100-, 1000-, and 10,000-ppm daidzein for 10 days. Plasma and egg yolk equol concentration was significantly increased with dietary daidzein supplementation. Gene abundance of two major bacterial enzymes catalyzing the conversion of daidzein to equol, dihydrodaidzein reductase, and tetrahydro daidzein reductase was measured using polymerase chain reaction (PCR) analysis, with the abundance increasing with dietary daidzein supplementation (p < 0.05). After sequencing the PCR amplicons, six possible bacteria were identified, and they were further identified and quantified using a specific primer for each bacterial gene. As a result, Adlercreutzia equolifaciens and Slackia equolifaciens were identified, and their bacterial relative abundance increased with dietary daidzein concentration and plasma equol levels (p < 0.05). A. equolifaciens and S. equolifaciens are known as equol-producing bacteria, and the present study was the first to show that these bacteria could also contribute to equol production in chickens.First published: 31 May 2025journal articl
19世紀における知識人と学知 : 「知」の創成から政治参与へ
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教育研究推進室主催の行事（FD・ワークショップ・その他）
Get PDF教育研究推進室の活動報告. 教育研究推進室主催の行事（FD・ワークショップ・その他）. FD・ワークショップ・その他一覧（2024年度）, 自己紹介の会開催一覧（2024年度）departmental bulletin pape
教員の自著紹介 : 伊藤伸幸 Nobuyuki ITO. Investigaciones arqueológicas en el área, de Tazumal dela zona arqueológica de Chalchuapa: proyecto arqueológico de El Salvador. Editorial Universidad Tecnológica de El Salvador, 2024.
Get PDF人文学研究科の教育・研究活動. 教員の著書.departmental bulletin pape
令和6年度人文学研究科研究プロジェクト 真福寺大須文庫のアーカイヴ創成プロジェクト : 今後の宗教遺産テクスト学の発展に向けて
Get PDF人文学研究科の教育・研究活動. 各種報告. 大学院教育の国際化に向けてdepartmental bulletin pape