Kochi University Repository

平野, 晋吾

岩崎, 美和

寺田, 信一

Shingo, Hirano

Miwa, Iwasaki

Shin-ichi, Terada

The Daily Rhythm Improvements on the Basis of Actual Daily Activities for Autism Spectrum Disorders: An Introductory Investigations

徳弘, 千恵

北村, 和子

稲田, 俊治

藤田, 尚文

chie, TOKUHIRO

Kazuko, KITAMURA

Toshiharu, INADA

Naofumi, FUJITA

The Relationship among the Students' Lifestyle, the Habitual Physical Exercise and the Self-esteem in Kochi Prefecture

是永, かな子

Kanako, KORENAGA

Educational Method for Inclusive Education in Regular Schools: Focusing on the LP Model in Norway and the Gifted Program in Denmark

原崎, 道彦

Michihiko, Harasaki

Relaxation as Buddhism

武久, 康高

Takehisa, Yasutaka

"Isemonogatari" The Feature of Expression in Chapter Twenty-One

今井, 多衣子

池, 純子

東條, 美紀

尾中, 美代子

高知大学農学部の日本事情科目　－体験型日本事情への取り組みー

小笠原, 光成

Gold-thioglucose と　high fat diet にて作製した非アルコール性脂肪肝炎(NASH) model mouse

Masayuki, Ishihara

Masayuki, Bun

Masayuki, Hisa

Yoshiko, Shimamura

Koji, Ogata

Kosuke, Inoue

Toru, Kagawa

Toshihiro, Takao

Yoshio, Terada

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5.Two independent pathways, BNIP3 and sestrin2, mediate autophagy of renal tubular cells in acute 
kidney injury in vitro and in vivo 
Masayuki Ishihara1), Masayuki Bun1), Masayuki Hisa1), Yoshiko Shimamura1), Koji Ogata1),
Kosuke Inoue1), Toru Kagawa1), Toshihiro Takao1), Yoshio Terada1)
1)Department of Endocrinology, Metabolism and Nephrology,  
2)Division of Community Medicine, Department of Community Nursing, Kochi Medical School, Kochi 
University
Autophagy is one of the systems which protect life from many kinds of stresses. In the previous study we 
reported autophagy occurred in acute kidney injury (AKI) mice model. Autophagy is thought to play a protective 
role in renal tubules from oxidative stresses. However, little is known about signal transduction for autophagy in 
AKI. Bcl-2/adenovirus E1B 19kDa-interacting protein 3 (BNIP3) is one of the target proteins of hypoxia 
inducible factor -1a (HIF-1a). Sestrin2 is one of the target proteins of p53. Both BNIP3 and sestrin2 have 
relations to injured cell survival. The aim of this study is to reveal the roles of BNIP3 and sestrin2 in autophagic 
pathway in AKI.  
 We used rat ischemia/reperfusion (I/R) AKI model in vivo and cultured renal tubular cells as an in vitro AKI 
model. The expression of BNIP3 and sestrin2 are similarly up-regulated 12-24h after I/R in proximal tubules in 
immunostaining and immunoblotting. BNIP3 mRNA and protein expressions were up-regulated in the hypoxia 
condition via HIF-1a dependently in vivo experiment. On the other hand, sestrin2 mRNA and protein expressions 
were up-regulated in the oxidative stress condition (H2O2) via p53 dependently. Furthermore, to examine BNIP3 
and sestrin2 regulate autophagy or not, we established NRK cells which stably transfected with a fusion protein 
between green fluorescent protein and light chain 3 (LC3-GFP) as a marker of autophagy. Overexpression of 
BNIP3 and sestrin2 both induced autophagy in NRK-LC3-GFP cells and induced LC3 protein expression. 
Interestingly, the autophagosome induced by BNIP3 localized mainly in mitochondria. Induction of autophagy by 
hypoxia was significantly reduced by sestrin2 siRNA. Meanwhile, induction of autophagy by H2O2 was reduced 
mainly by BNIP3 siRNA. These observations disclose that autophagy in renal tubules in AKI is induced by at 
least two independent pathways, p53-sestrin2 pathway and HIF-1a-BNIP3 pathway. These two pathways may 
work differently according to the types of stresses to protect renal tubules in AKI. 


Two independent pathways, BNIP3 and sestrin2, mediate autophagy of renal tubular cells in acute kidney injury in vitro and in vivo

村上, 武

氏原, 友三郎

佐藤, 一石

岡島, 邦彦

カオリナイト系粒状セラミックス処理水を利用した透析システム清浄化の試み

海部, 忍

沖田, 学

山手, 千里

三笠, 洋明

椛, 秀人

左半側空間無視患者における視覚情報の有無が頚部の回旋角度に及ぼす影響

Kōchi University

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