Kochi University Repository

上岡, 克己

文学作品を授業に活かす　－「英語読解研究演習」試論－

Doyle, Howard

ドイル, ハワード

"Contact With" and "Use Of" English in Different Circles: a 3-case comparison

北村, 嘉久

山口, 俊博

Yoshihisa, KITAMURA

Toshihiro, YAMAGUCHI

The based graphs of rational toral rank 4

神埼, 道太郎

経済産業省アジア人財資金構想ビジネス日本語をめぐって　－高知大学での現状と課題を中心にー

王, 宇杰

奥谷, 文乃

椛, 秀人

ヒストン脱アセチル化抑制は幼若ラットの嗅覚嫌悪学習を促進する

野村, 晴香

川崎, 綾

福田, 順子

岩崎, 修美

山口, 真

内田, 勝幸

吾妻, 健

プレバイオテックス導入による便秘及び便臭の改善評価

小森, 正博

上原, 良雄

小林, 泰輔

兵頭, 政光

慢性中耳炎から分離されたブドウ球菌の遺伝子解析　－健常者の保菌するブドウ球菌との比較－

Mikiro, Takaishi

Junji, Takeda

Shigetoshi, Sano

㧙  㧙
22.Induction of mesenchymal-epithelial transition in fibroblast-like spindle cancer cells by reprograming 
factors
Mikiro Takaishi1), Junji Takeda2), Shigetoshi Sano1)
1) Dept. of Dermatology, Kochi Medical School, Kochi Univ. 
2) Dept. of Social and Environmental Medicine, Graduate School of Medicine, Osaka Univ. 
It was reported that induced pluripotent stem (iPS) cells went through mesenchymal-epithelial transition (MET) 
when they were generated from embryonic fibroblasts by introduction of reprogramming genes. The results 
prompted us to study whether cancer cells under epithelial-mesenchymal transition (EMT) could be reversed by 
the reprogramming genes. To this end, we used fibroblast-like squamous cell cancer (SCC) lines derived from 
mouse skin, and the piggyBac transposon system to deliver the reprogramming factors (Oct3/4, Sox2, Klf4, 
c-Myc and Lin28), which were linked by self-cleaving peptides. The piggyBac transposon and piggyBac 
transposase expression vector were co-transfected into spindle-shaped SCC cells. The stable transformants were 
obtained after the puromycin selection, and they showed polygonal morphology with well-established cell-cell 
adhesion. Quantitative RT-PCR and immunoblotting revealed increased epithelial markers and reduced 
mesenchymal markers in the transformed cells compared with the parental SCC cells. Also, the transformed cells 
showed N-cadherin at the cell-cell border and actin bundles, similar to those found in epithelial cells. Cell 
proliferation and cell invasion activity were reduced in the transfected cells. The EMT in human SCC cells also 
showed a reversal with the reprogramming genes. Taken collectively, these results suggested that a malignant 
process associated with EMT was reversed by introduction of the reprogramming genes for iPS cells. This 
finding provides an idea for the potential therapeutic use of the reprogramming genes to attenuate malignant 
nature of metastatic cancer. 


Induction of mesenchymal-epithelial transition in fibroblast-like spindle cancer cells by reprograming factors

Chie,  Nishioka

Takayuki, Ikezoe

Mutsuo, Furihata

Jing, Yang

Satoshi, Serada

Tetsuji, Naka

Atsuya, Nobumoto

Masayuki, Tsuda

Keiko, Udaka

Akihito, Yokoyama

㧙  㧙
27.CD34+/CD38- acute myelogenous leukemia cells aberrantly express CD82 adhesion molecule. 
Chie Nishioka1), 6) , Takayuki Ikezoe2), Mutsuo Furihata3), Jing Yang2), Satoshi Serada4), Tetsuji Naka4), Atsuya 
Nobumoto5), Masayuki Tsuda5), Keiko Udaka1) and Akihito Yokoyama 2) 
1) Department of immunology, 2) Hematology and Respiratory Medicine, 3)Tumor Pathology,  
5) The Facility for Animal Research, Kochi Medical School,  
4) Laboratory for immune Signal, National Institute of Biomedical Innovation,  
6) Research Fellow of the Japanese Society for the Promotion of Science (JSPS)  
To identify molecular targets in leukemia stem cells (LSCs), this study compared the protein expression profile of 
freshly isolated CD34+/CD38- cells with that of CD34+/CD38+ counterparts from individuals with acute 
myelogenous leukemia (n=2, AML) using isobaric tags for relative and absolute quantitation (iTRAQ). A total of 
98 proteins were overexpressed, while six proteins were underexpressed in CD34+/CD38- AML cells compared 
with their CD34+/CD38+ counterparts. Proteins overexpressed in CD34+/CD38- AML cells included a number of 
proteins involved in DNA repair, cell cycle arrest, gland differentiation, anti-apoptosis, adhesion, and drug 
resistance. Aberrant expression of CD82, a family of adhesion molecules, in CD34+/CD38- AML cells was noted 
in additional clinical samples (n=12) by flow cytometry. Importantly, down-regulation of CD82 in CD34+/CD38-
AML cells by a short hairpin RNA (shRNA) stimulated their migration via up-regulation of matrix 
metalloproteinases 9 (MMP9), as assessed by transwell assay and real time RT-PCR, respectively. Moreover, we 
found that down-regulation of CD82 in CD34+/CD38- AML cells by an shRNA significantly impaired 
engraftment of these cells in severely immunocompromised mice. Taken together, aberrant expression of CD82 
might play a role in adhesion of LSCs to bone marrow microenvironment. CD82 could be an attractive molecule 
target to eradicate LSCs. 


CD34+/CD38- acute myelogenous leukemia cells aberrantly express CD82 adhesion molecule.

Kōchi University

Not a member yet