Kochi University Repository

藤吉, 清次郎

Seijiro, Fujiyoshi

"Wakefield"論　-ウェイクフィールド夫人の人物造型を中心に-

高橋, 克己

Katsumi, Takahashi

Ἰουλιανοῦ «Κοινὴ Ἔννοια» Iuliani «Communis Sententia»：ユーリアーノスの「共通(コイネー・)観念(エンノイア)」：Julians «Common Notion»

是永, かな子

真城, 知己

KORENAGA, Kanako

SANAGI, Tomomi

デンマークにおける地方分権とインクルーシブ教育の実際

安東, 知子

森田, 美佐

ANDOU, Tomoko

MORITA, Misa

A Study of Present and Futura Attitudes about Japanese Gender Roles

Antonino, B Mendoza, Jr.

Victor , S. Soliman

Community Structure of Macroalgae of Lagonoy Gulf, Bicol Region, Philippines

Raul, G. Bradecina

Teruyuki, Shinbo

Plutomeo, M. Nieves

Exploring Local Coastal Recreational Tourism as a Potential Strategy to Support Changing Agriculture in the Typhoon-Prone Small Island of San Miguel, Tabaco, Albay, Bicol Region

岡村, 慶

岡村, 千恵子

Kei, Okamura

Chieko, Okamura

アメリカ合衆国の初等～中等教育における学校段階区分の変遷について

«Οἱ τὰ Εἴδη Τιθέμενοι» «Qui Species Constituunt»：「諸形(エイ)相(ドス)を[重要と]看做す者達」：«Proponents of the Forms»

«Ὑπερούσιος Θεός» «Superessentialis Deus»：「超実在[実体](ヒュぺル・ウースィオス)の神(テオス)」：«Superessential God»

平成25年11月26日授与　高知大学甲医博第150号　山田 るりこ氏学位申請論文要約原文掲載は、European Journal of Obstetrics Gynecology and Reproductive Biology　，171（1）, November 2013, Pages 96-100　博士（医学）高知大

山田, るりこ

                                                         Yamada R. et al. 
 1
 
 
Is it possible to diagnose of malignancy from fluid in Cystic 
Ovarian Tumors? 
 
 
 
 
Ruriko Yamada,1 Nagamasa Maeda,1 Hiroyoshi Oguri,1 Yoshihiro Adachi,2 Tamotsu 
Takeuchi,2 Mutsuo Furihata, 2 and Takao Fukaya1  
 
1Department of Obstetrics and Gynecology and 2Department of Pathology, 
Kochi Medical School, Kochi, Japan 
 
 
All correspondence concerning this paper should be addressed to: 
Nagamasa Maeda, M.D. Ph.D. 
Department of Obstetrics and Gynecology 
Kochi Medical School, Kohasu, Oko, Nankoku, 
Kochi, 783-8505, Japan 
Telephone: +81-88-880-2383; Fax: +81-88-880-2384 
E-mail:maedan@kochi-u.ac.jp 
                                                         Yamada R. et al. 
 2
 
INTRODUCTION 
 
Improvements in diagnostic accuracy are needed for treatment of ovarian tumor.  
The treatment of ovarian cystic tumors remains some problems. Should they be 
removed because of the carcinoma risk or should they be left in order to preserve 
fertility? The establishment of a detection system for gene mutations in cystic fluid 
might help to determine malignancy without requiring the resection of the tumor 
tissues. 
     We investigated p53 gene expression and its mutation in the cystic fluid from 
patients with benign and malignant tumors to determine whether it reflects the 
molecular status of the cyst wall tissue. 
 
 
SUBJECTS AND METHODS 
 
  Forty-eight patients with ovarian cystic tumors participated in this study. All patients 
had a laparotomy or laparoscopic surgery at the Department of Obstetrics and 
Gynecology, Kochi Medical School, from April 2004 to December 2011. The 48 
patients included 31 with benign cysts, 10 with malignant tumors and 7 with borderline 
malignancy. This study was approved by the Institutional Review Board of Kochi 
Medical School Hospital. 
                                                         Yamada R. et al. 
 3
     Ovarian tumor tissue, the corresponding ovarian cystic fluid, and peripheral 
blood (PB) samples were obtained at surgery. The PB and ovarian cystic fluid samples 
were frozen and stored at -80 until DNA extraction. 
After DNA extraction from the cystic fluid, polymerase chain reaction (PCR) 
and sequence analysis for exons 4-9 of the p53 was performed. In two cases of 
mucinous cystic tumor of borderline malignancy and endometioid adenocarcinoma, the 
p53 gene sequencies were determined. Furthermore, immunohistochemical staning for 
abnormal p53 gene product was performed. 
 
 
RESULTS 
 
     DNA was successfully extracted from all cystic fluid specimens of at least 1 ml 
regardless of the histlogical types of the tumor. After 35 cycles of PCR on extracted 
DNA using each set of primers, exons 4-9 of p53 gene were identified by 
electrophoresis for all types of ovarian cyst. 
     In one case with a mucinous cystic tumor of borderline malignancy, a point 
mutation in exon 6 at codon 223 (CCT→CTT) was detected by the direct sequencing of 
the amplified Exon. Notably, the mutation was not present in the PB sample from the 
patient. 
     In another case with a endometrial adenocarcinoma, a point mutation in exon 7 
at codon 245 (GGC→AGC) was detected by the direct sequencing of the amplified 
                                                         Yamada R. et al. 
 4
Exon. Notably, the mutation was not present in the PB sample and tissue specimens 
from the patient. 
In our study, there is no p53 mutation in both cystic fluid and tissue from 
benign ovarian cyst. p53 mutation were identified in 12.5% (1/8) both of cystic fluid 
and tissue from borderline malignancy. They were identified in 10% (1/10) of cystic 
fluid with malignancy, but there is no mutation of p53 in tissue from malignancy. 
 
 
CONCLUSION 
 
     Concerning with cystic ovarian tumors, cystic fluid may provide informative 
material for molecular studies since it reflects the p53 status of tumor tissue on the cyst 
wall. We conclude that further gene studies may contribute to establish a new detection 
system for the presence of malignancies of cystic ovarian tumors. This system might 
help to identify ovarian malignant without resection of the tumor tissues. 


Is it possible to diagnose of malignancy from fluid in Cystic Ovarian Tumors?

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