137262 research outputs found
Sort by
Non-Surgical Submarginal Instrumentation of Peri-Implant Mucositis With Delivery of Sodium Hypochlorite/Amino Acids and Cross-Linked Hyaluronic Acid: A Randomized Clinical Trial
No full textObjectives: To evaluate the effects of submarginal instrumentation (SI) with or without adjunctive delivery of sodium hypochlorite (NaOCl)/amino acids and cross-linked hyaluronic acid (xHyA) gel in the treatment of peri-implant mucositis (PM). Material and methods: Forty implants supporting single-unit crowns diagnosed with PM in 40 patients were randomly assigned to test (SI + NaOCl/amino acids and xHyA) or control group (SI alone). The primary outcome was mean BoP change. Full-Mouth Plaque Score (FMPS), Full-Mouth Bleeding Score (FMBS), modified plaque index (mPlI), and probing depth (PD) were assessed as secondary outcomes. Clinical parameters were assessed at baseline, 3 and 6 months. Disease resolution was also recorded. Results: Two patients were lost during follow-up while 38 patients completed the study without adverse effects. After 6 months, all clinical parameters improved statistically significantly in both groups (p 0.05); conversely, an initial PD = 5 mm yielded a statistically significant difference (p < 0.05). Disease resolution correlation with test group was statistically significant with a 3.77 odds ratio. Conclusion: Within the limitations of the present study, adjunctive delivery of NaOCl/amino acids and xHyA to SI yielded superior clinical outcomes compared with SI alone in the treatment of PM. Trial registration: ClinicalTrials.gov: NCT05926297
DBT_DCNN: a new convolutional neural network for mass detection in digital breast tomosynthesis
No full textPurpose
We developed DeepLook, a CAD based on a CNN for the analysis of Digital Breast Tomosynthesis images to classify and locate possible breast mass lesions.
Methods
This CAD takes advantage of a pre-processing algorithm to remove pectoral muscle and skin, before entering the lesion classification step and the use of a CNN architecture. Evaluation of the architecture performance was realized using a dataset coming from Duke University, one of the largest databases publicly available online.
Results
DeepLook can classify individual DBT images into two classes: healthy (Negative) and presence of a mass (Positive) or in three classes: healthy, presence of a malignant or benignant masses. Accuracy and sensitivity on the binary classification of test data of the most suitable considered dataset reached 82% and 68%, respectively, with an AUC of 0.90.
Conclusions
We developed DeepLook, a CAD based on a CNN for the analysis of DBT images to classify and locate possible breast mass lesions. The reported performance was obtained with a CNN architecture that is significantly simpler than the ones proposed in the literature so far and is promising for future clinical implementation in routine DBT diagnosis, so as to suggest to radiologists the slices that are worth paying attention to better. As a further step, a statistical study of the mass locations in images generated through the GradCam algorithm is under development
Master di secondo livello “Studi di genere, Educazione alle differenze, politiche di uguaglianza”, del Dipartimento di Studi Umanistici dell’Università degli Studi di Napoli Federico II;
No full textA simple and rapid method for generating antibodies against bovine alphaherpesvirus 1 viral proteins through immunization with virus-infected murine cells
No full textBovine alphaherpesvirus 1 (BoAHV-1) infects cattle and typically results in significant economic losses for the
cattle industry worldwide. Currently, antibodies targeting only a limited number of viral proteins are
commercially available. It has been reported that BoAHV-1 is capable of infecting numerous tumor cell lines.
Based on the rationale of immune tolerance, we hypothesized that virus-infected murine cells could be directly
used to immunize mice, thereby generating antibodies against viral proteins. In this study, we found that BoAHV-
1 can infect murine cell lines including LA795 and MC38, as determined using both Western blot and immu
nofluorescence analyses. Immunizing mice with virus-infected cells, either through subcutaneous or intraperi
toneal injection, stimulates the production of high levels of antibodies that specifically recognize the viral
proteins synthesized in bovine kidney (MDBK) cells, as characterized by both Western blot and/or immunoflu
orescence. Furthermore, our findings suggest that intraperitoneal immunization could more effectively elicit
antibodies against a wider array of viral proteins. As a homemade antibody generation method, this approach
bypasses the complex and time-consuming steps of producing and purifying recombinant proteins as antigen,
which are typically performed in conventional methods for antibody generation. Thus, we present a simple,
rapid, and cost-effective method for generating virus-specific antibodies
Liquid biopsy and tissue biopsy for the detection of EGFR mutations in patients with stage III non-small-cell lung cancer: an observational real-world study
No full textBackground: In metastatic non-small-cell lung cancer (NSCLC), tissue and plasma (liquid biopsy) testing are recommended complementary approaches to identify actionable gene mutations. In non-metastatic disease, the utility of liquid biopsy is less clear. This retrospective observational study assessed the effectiveness of liquid biopsy to detect epidermal growth factor receptor (EGFR) mutations in stage IIIA/IIIB/IIIC NSCLC against tissue biopsy. Patients and methods: Data from a US nationwide electronic health record-derived deidentified database of patients with NSCLC and liquid and tissue biopsy test results for EGFR mutations were analyzed from time of stage III NSCLC diagnosis (1 January 2017 to 31 August, 2023) until the data cut-off or date of metastasis (if the tumor metastasized). Objectives were to assess concordance and performance of liquid and tissue biopsy tests for the detection of EGFR mutations. Results: Data were analyzed from 425 patients. Some 43%, 41%, and 9% of patients had stage IIIA, IIIB, and IIIC NSCLC, respectively; 27% had unresectable NSCLC. For all EGFR mutation testing methods, median time between specimen collection and test result availability was 8 days for liquid biopsy (n = 494) and 32 days for tissue biopsy (n = 499); 25 (5%) and 41 (8%) samples, respectively, detected EGFR mutations. The concordance rate was 93% overall and was greater for tests conducted in 2020-2023 (96%) than 2017-2019 (87%). Specificity and sensitivity of liquid biopsy were 98% and 45%, respectively. Next-generation sequencing (NGS) was the most common biopsy testing method (liquid, 74%; tissue, 67%). For NGS tests only, concordance was 94% (2020-2023, 96%; 2017-2019, 85%), with a specificity and sensitivity of 99% and 53%, respectively. Conclusions: Liquid biopsy may complement tissue biopsy in detection of EGFR mutations in stage III NSCLC. Using current NGS technology, EGFR mutation status was identified with high specificity. Further research is warranted to confirm the utility of liquid biopsy for these patients
Tra tolleranza e divieto: la costruzione giuridica delle immissioni
No full textDal «celebre apostegma» di Aristone riferito da Ulpiano (D. 8.5.8.5): in suo enim alii hactenus facere licet, quatenus nihil in alienum immittat, considerata da Labruna una fra le più celebri e discusse leggi delle Pandette», alla prassi giudiziaria postunitaria, alla norma codicistica introdotta con il compito di definire parametri, soglie e criteri di valutazione delle immissioni tollerabili
