Hungarian Veterinary Archive
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Comparison of in situmeasured plasma lactate-levels during standardised exercise in high water aquatrainer and on treadmill in show jumpers
SUMMARY
Background: A few data is available in the literature focusing on the changes of in situ measured biochemical parameters that compare differences between aqua and dry treadmill. Objectives: The aim of the study was to investigate how the underwater exercise modifies the changes of plasma lactate of the horse during training.Materials and Methods: Measurements were made on 4 normally trained show jumpers within a 4 days long period (1st day: warm up, 2–4th day: training, 1 week rest between the two periods). The blood samples for plasma lactate determination were collected during exercise through a permanent intravenous catheter in the jugular vein.Results and Discussion: 1. The average of the minimum values of all horses was significantly (p = 0.017) lower on aqua than on dry device but no significant dif-ference presented between the average of the maximum values (p = 0.943). The average of cumulated lactate level (T/1–T/5 samples, 2–4th day) was almost sig-nificantly lower on aqua than on dry unit (p = 0.058), but no significant difference was measured in the average of cumulated data from samples T/6–T/8. 2. The day after day cumulated data analysis did not show significant differences concerning the average of the minimum and maximum values and the average of samples T/1–T/5 on each day from 2–4th day. The average of samples T/6–T/8 was significantly (p = 0.033) higher on aqua on the 2nd day. 3. The maximum values in water were higher on the 2-3rd day and lower on the 4th day than on dry treadmill. Trends of day after day individually cumulated values from T/1–T/5: in water values were lower on each day except two days (almost equal).Differences considering the individuals were much more obvious than in cumulated analysis, especially when the evaluation of the sampling times was divided into two parts. The exercise seems to be more intensive in aquatrainer, based on the higher maximum post-training values. The appropriate temperature, the more intensive flexor-extensor exercise, the massage effect of water and increased capillary activity could be important factors to decrease the lactate level during aquatraining
The relevance of the oxidative stress and monitoring options in dairy cattle herds - Literature review
SUMMARY
In this review article, the authors summarize the results of several scientific research on the oxidative stress (OS), its occurrence in cattle herds and its physiological and economical effects. OS is developed, when the concentration of reactive oxygen species exceeds the capacity of the natural antioxidant defence system in the body. The production of reactive oxygen species is continuous and avoidable in aerobic organisms, but normally it is under control, while formation of these reactive materials is inhibited, or they will be eliminated by natural antioxidants immediately. When this control mechanisms are disturbed, OS can develop. As the main predisposing factors for OS are the metabolic stress, the heat stress, the up taken mycotoxin contaminated feed, inflammatory processes and some physiological events like the birth and the calving, the OS can be expected for any herd at any time, due to these factors occur frequently. The OS is associated with several diseases of dairy cows, like ketosis and insulin resistance, retained foetal membranes, metritis, mastitis, udder oedema, early embryonic mortality, ovarian disfunction etc. The OS-caused major negative consequences may generate animal discomfort and significant economic losses for farmers. These major consequences primarily originate from the damage of biologic structures like proteins, lipids and nucleotides and can cause cell-level disfunctions which may be manifested in a reduced fertility and productivity. These consequences are also overviewed in this article in detail. The practical use of currently available individual diagnostic possibilities of OS is limited in farm conditions. This is highlighting the need to develop a routine, herd-level in vivo diagnostic methods for monitoring OS. The research on OS herd monitoring methods is still in progress at the University of Veterinary Medicine, Department of Animal Hygiene, Herd-health and Mobile Clinic
Absorption and accumulation of the D-tryptophan content of foodstuffs in the inner organs of the rats
SUMMARY
Background: Because of the difficulty in determining D-tryptophan, the total content of D-tryptophan in the internal organs of the rats was not studied before. A new protein hydrolysis method, free of racemization, allowed us to measure the absorption and accumulation of D-tryptophan in the rat’s internal organs.Objectives: After the appropriate analytical method was available, different amounts of D-Trp were fed to rats and tested for its absorption and accumulation in certain (spleen, liver, kidney) organs of the animals.Materials and methods: We applied one-time and continuous D-Trp dosing in 200 and 400, and 100, 200, 350 and 600 mg/bwkg, respectively, where the D-amino acid was mixed into the feeding stuff. After the feeding period (three weeks) was over, samples were taken from the kidney, liver, and spleen, and the total D-Trp content were measured. Results and discussion: Following a one-time D-Trp input, D-Trp cannot be detected in the organs, like in the case of the control animals. The weight gain of the animals was the same as for the control animals, which is supported by the fact, that rats can utilize D-Trp the same effectiveness as the L-enantiomer. For the kidney from the 100, for the liver from the 200 mg/bwkg dosage made the organs’ D-Trp content measurable. In case of every treatment the ratio of D-Trp is the highest in the spleen, and by increasing the dosage, the differ-ence between the D-Trp% measured in the spleen and the other two organs is increasing. In case of kidney, D-Trp can be detected for lower doses, for the three highest doses we cannot differentiate between the organs and the doses.The accumulation least affected the liver, because the D-amino acid oxidase system is taking place there. Kidney was a bit more sensitive for the presence of D-Trp, because the studied amino acid can be detected in case of smaller doses. For higher doses kidney did not show a remarkable difference from liver, and the highest accumulation could be found in the spleen, because in this organ there is no special procedure taking place for the removal of D-amino acids
Prof. Dr. Sugár László állatorvos a Magyar Érdemrend Lovagkeresztje kitüntetést vehette át Dr. Nagy István miniszter úrtól
Sertés vágóhídi mintákból származó Escherichia coli törzsek antibiotikum-rezisztenciájának felmérése
Napjaink egyik legmeghatározóbb, az egész világot érintő problémája az egyre
nagyobb mértékben terjedő antibiotikum-rezisztencia. A folyamat eredményeképpen az
antibiotikum kezelésnek ellenálló, ún. multirezisztens baktériumtörzsek jelennek meg a
humán- és az állategészségügyben. A törzsek kiszelektálódásának főinduktora a helytelen,
szubterápiás dózisban alkalmazott antibakteriális kezelések. A nem megfelelő ideig
alkalmazott vagy eleve nem a legmegfelelőbb hatóanyag választása jelentősen megemeli a
rezisztens törzsek terjedését
Examination on microorganism profile of cows bulk milk with a focus on mastitis pathogens
A tőgygyulladás világszerte jelentős veszteségeket okoz a tejiparban, nem csak a tejmennyiség csökkenése, hanem a megtermelt tej minőségének romlása miatt is. A tejben található egyes baktériumok meggyorsítják a tej romlását, befolyásolják a készülő tejtermék ízét, állagát, egyéb organoleptikus tulajdonságait és eltarthatóságát.
A kutatás célja a magyar árutejben előforduló tőgypatogén kórokozók kimutatása volt. A mintavétel kevert mintából, tanktejből történt az ország több pontján található tehéntelepekről. A vizsgálat során a poolozásnak köszönhetően a magyarországi telepek jelentős részét, szinte minden tejtermelő telepet sikerült lefedni. A vizsgált kórokozók listáját a Streptococcus uberis, S. agalactiae, S. dysgalactiae, Staphylococcus aureus, Staph. epidermidis, Staph. hyicus, Staph. chromogenes, Staph. simulans, Staph. warneri, Staph. xylosus, Staph. sciuri, Corynebacterium bovis, Prototheca zopfii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa alkotja