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Association between NF-E2-related factor 2 polymorphism and age-related hearing loss in the general Japanese population from the Iwaki health promotion project
Get PDFBackground: Single-nucleotide polymorphisms (SNPs) of the NF-E2-related factor 2 (NRF2) gene might be associated with several diseases. In the animal models, NRF2 has been reported to be associated with the age-related hearing loss (ARHL). However, the effect of NRF2 SNPs on ARHL in the general population has not been reported.
Objectives: This study aimed to evaluate the effect of NRF2 SNP-617 on ARHL in the general Japanese population.
Methods: This study included participants from the Iwaki Health Promotion Project. The genotypes of NRF2 SNP-617 were determined via whole-genome sequencing, and the hearing level of the CC homozygote+CA heterozygote carrier group was compared with that of the AA homozygote carrier group.
Results: Among the 306 CC+CA and 31 AA group male subjects, 22 (7.2%) and six (19.4%), respectively, had hearing loss, with a significant difference. Multiple logistic regression analysis showed that the risk of hearing loss in the male subjects was significantly higher in the AA group than that in the CC+CA group (p=0.031, odds ratio=3.437, 95% confidence interval=1.116–10.580).
Conclusion: The genotypes of NRF2 SNP-617 AA carriers were significantly associated with the development of ARHL among the males aged 30–59 years.弘前大学博士（医学）Author: Takashi Kasai, Akira Sasaki, Shuya Kasai, Shiori Miura, Shinichi Goto, Ryoko Yotsuyanagi, Tatsuya Mikami, Yoshinori Tamada, Ken Itoh, Atsushi Matsubara
Acta Oto-Laryngologica. 2025 Jan;145(1):36-42. doi: 10.1080/00016489.2024.2435459. Epub 2024 Dec 6.
http://creativecommons.org/licenses/by-nc-nd/4.0/deed.j
Effects of rewarming therapies on outcomes in accidental hypothermia: A secondary analysis of a multicenter prospective study
Get PDF弘前大学博士（医学）Author(s): Kana Sugiyama, Osamu Nomura, Jin Irie, Yoshiya Ishizawa, Shuhei Takauji, Mineji Hayakawa, Yoshinori Tamada, Hiroyuki Hanad
Characteristics of extracellular miR-21a-5p expression in aging and senescence
Get PDF弘前大学博士（保健学）学位論文のもととなる研究成果としての筆頭著者原著
論文題目：MicroRNA‑21‑5p expression in extracellular vesicles is increased in the blood of aging mice and in vascular endothelial cells induced by ionizing radiation
掲載学術誌（巻号）：Experimental and Therapeutic Medicine （February-2025 Volume 29 Issue 2 Article Number: 22）
DOI：https://doi.org/10.3892/etm.2024.12772学位論文のもととなる研究成果としての筆頭著者原著
論文題目：Changes in cognitive ability and serum microRNA levels during aging in mice
掲載学術誌（巻号）：Experimental and Therapeutic Medicine （March-2024 Volume 27 Issue 3 Article Number: 120）
DOI：https://doi.org/10.3892/etm.2024.12408学位論文のもととなる研究成果としての筆頭著者原著
論文題目：Examination and comparison of the RNA extraction methods using mouse serum
掲載学術誌（巻号）：Biomedical Reports（March-2024 Volume 20 Issue 3 Article Number: 51）
DOI：https://doi.org/10.3892/br.2024.173
Distal radioulnar joint kinematics evaluated using ultrasonography during handstanding in female gymnasts
No full textBackground: Wrist pain prevalence is significantly higher in female gymnasts than in male gymnasts, and the pathological site varies depending on patient age. However, the differences between age groups have not been elucidated with a focus on changes in the kinematics of the distal radioulnar joint (DRUJ).
Purpose: To analyze the effect of wrist weightbearing during a handstand on the kinematics of the DRUJ using ultrasonography in female elementary school (ES), junior high school (JH), and high school (HS) gymnasts, and to determine whether wrist pain affects the kinematic measurements in female gymnasts.
Study Design: Descriptive laboratory study.
Methods: A total of 66 wrist joints in 33 female gymnasts (5 ES, 9 JH, and 19 HS students) were included. Ultrasound examinations were performed in neutral extension, nonweightbearing (NENWB); full extension, nonweightbearing; and full extension, weightbearing (FEWB). The radioulnar joint kinematics were evaluated by measuring the radioulnar separation and dorsivolar shift. The change in distance in the FEWB condition was determined as a reference for those in the NENWB condition and for comparison between the 3 school groups. Also, the change in distance was compared between the gymnasts with and without wrist pain.
Results: The radioulnar separation increased from the NENWB to the FEWB condition in all 3 groups, and a significantly larger change in distance was observed in HS students compared with ES students (P = .008). The dorsivolar shift decreased from the NENWB to the FEWB condition in ES and JH students; it was almost unchanged in HS students. There was a significant difference in change in dorsivolar shift from the NENWB to the FEWB condition between ES and JH students (P = .023). There were 2 ES, 2 JH, and 10 HS students with wrist pain. The change in radioulnar separation was significantly greater separation in gymnasts with wrist pain compared with those without wrist pain (P < .001).
Conclusion: The radioulnar joint kinematics during the FEWB condition varied with school age in female gymnasts. The larger increase in the radioulnar separation from the NENWB condition to the FEWB condition might be associated with a biomechanical mechanism of wrist pain in female gymnasts.
Clinical Relevance: Repetitive tensile loads put female gymnasts at risk of wrist pain and overuse injury.弘前大学博士（医学）Author(s): Nishimura, Shinya OT, BS / Wabiko, Yuki OT, BS / Miura, Hiroyuki OT, MS / Kamiharako, Aina OT / Tsuda, Eiichi MD, Ph
Association between oral bacterial species and halitosis: findings from a study of a community-based Japanese population
No full textHalitosis is known to be associated with oral bacteria; however, its specific relationship with particular bacterial species within the oral microbiota remains uncertain. Our objective was to identify oral bacterial species associated with volatile sulfur compound (VSC) production that contribute to halitosis in a community-based Japanese population. This study included 1018 participants. Tongue plaque samples were collected and the oral microbiome was analyzed via 16S rDNA amplicon sequencing. Participants with VSC levels greater than 250 ppb were categorized as having oral malodor. Linear discriminant analysis effect size was used to compare bacterial compositions between participants with and without halitosis. In this study, we identified 37 bacterial genera in tongue plaque samples. Significant differences in bacterial composition were found between the malodor and control groups. Porphyromonas, Fusobacterium, and Solobacterium were more abundant in the malodor group, whereas Streptococcus and Rothia were more prevalent in the control group. Multiple regression analysis further revealed that Porphyromonas and Solobacterium abundances were positively correlated with oral malodor. We found that halitosis in this Japanese population is associated primarily with pathogenic periodontal bacteria (members of the red and orange complexes) and Solobacterium moorei. The bacterial community composition of individuals with halitosis differs significantly from that of healthy individuals, emphasizing the role of specific bacterial species in oral malodor development. These findings increase our understanding of the microbial basis of halitosis and suggest that targeting Solobacterium, along with treating periodontal disease, may be effective in combating halitosis.弘前大学博士（医学）Author(s): Komatsu, Shotaro ; Tamura, Yoshihiro ; Takagi, Koki ; Yamazaki, Shunya ; Matsumura, Akihiro ; Kubota, Kosei ; Narita, Norihiko ; Matsumiya, Tomoh ; Sawada, Kaori ; Mikami, Tatsuya ; Nakaji, Shigeyuki ; Kobayashi, Watar
Heterozygous troponin T-K210 del mutant iPSCs generated from a patient with familial dilated cardiomyopathy and CRISPR-Cas9 genome editing experiment
Get PDFBackground: The advent of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 （CRISPR-Cas9） system has markedly accelerated research employing induced pluripotent stem cells （iPSCs） for the analysis of gene function and gene therapy. In this study, we performed exome sequencing to identify genetic mutations in patients with familial dilated cardiomyopathy （DCM）. Subsequently, we generated iPSCs from the patient and performed genome editing experiment using the CRISPR-Cas9 system.
Methods and Results: Exome sequencing was conducted on genomic DNA extracted from two siblings with familial DCM. The results revealed the presence of the heterozygous troponin T （TnT）-K210 del mutation （ΔK210/WT） in both individuals. One of the patients’ peripheral blood mononuclear cells were reprogrammed into iPSCs through electroporation of episomal plasmids encoding the reprogramming genes. The ΔK210/WT iPSCs were successfully differentiated into cardiomyocytes. Despite the generation of homozygous TnT-K210 del mutant （ΔK210/ΔK210） iPSCs using the CRISPR-Cas9 system, the ΔK210/WT iPSCs could not be repaired into the WT/WT iPSCs.
Conclusions: The TnT-ΔK210/WT was detected in patients with DCM, and iPSCs were generated. The CRISPRCas9 system was employed to successfully generate the ΔK210/ΔK210 iPSCs, but not the WT/WT iPSCs from the ΔK210/WT iPSCs. Further studies are necessary to generate WT/WT, ΔK210/WT, and ΔK210/ΔK210 iPSCs.弘前大学博士（医学）Author: Toshihiro Iwasaki，Shuji Shibutani，Motoki Chiba，Mei Hiyama，Hitoshi Umezaki，Shun Hirosawa，Kazufumi Kato，Yuki Konno，Hirofumi Tomit
Impact of nephroureterectomy on postoperative renal function in upper tract urothelial carcinoma: A multicenter retrospective study
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Reactive oxygen species are involved in inhibition of photoreactivation of Staphylococcus aureus irradiated with 222-nm Far ultraviolet C
Get PDFUltraviolet-C (UV-C) at a wavelength of 254 nm is used for disinfection but cannot be used in dwelling space because it is harmful to the human body, while 222-nm Far UV-C shows germicidal effect and poses little hazardous effect to human. Formation of cyclobutane pyrimidine dimers (CPD) of DNA is a main mechanism of UV-C germicidal effect. CPD formed by irradiation with 254-nm UV-C is repaired and bacterial proliferation is recovered by photoreactivation. In this study, we investigated photoreactivation of Staphylococcus aureus irradiated with 222-nm Far UV-C. The proliferative effect of 222-nm Far UV-C irradiated S. aureus by photoreactivation was inferior to that of irradiated with 254-nm UV-C. The 254-nm UV-C wavelength and 222- nm Far UV-C induced CPD in S. aureus cells, and the same level of CPD was repaired in cells irradiated with either UV-C after photoreactivation. It has been reported that UV-C induces generation of reactive oxygen species (ROS) in bacteria, and that ROS oxidize and inactivate a variety of biomolecules in bacteria. The present study showed that more ROS-producing S. aureus were observed after irradiation with 222-nm Far UV-C compared with 254-nm UV-C. These results indicate that ROS may be involved in lower recovery of 222-nm Far UV-C irradiated S. aureus by photoreactivation.弘前大学博士（医学
A Novel Mixed Reality Training Program for Postoperative Nursing Care: Developing Clinical Judgment and Enhancing Practical Skills through Immersive Simulation
Get PDF弘前大学博士（保健学）学位論文のもととなる研究成果としての筆頭著者原著
論文題目：Development and Educational Effectiveness of a Mixed Reality (MR) Program to Support Clinical Judgment in the Observation of Postoperative Patients
掲載学術誌（巻号）：Healthcare 2024, 12(23), 2357
DOI： https://doi.org/10.3390/healthcare1223235
Examination of the effect of physical therapy combined with kinesiology taping on pain and muscle weakness in patients with knee osteoarthritis
Get PDF弘前大学博士（保健学）学位論文のもととなる研究成果としての筆頭著者原著
論文題目：Examination of the effect of physical therapy combined with kinesiology taping on pain and muscle weakness in patients with knee osteoarthritis
掲載学術誌（巻号）：Journal of Physical Therapy Science (2024 Volume 36 Issue 12 Pages 791-796)
DOI：https://doi.org/10.1589/jpts.36.79