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Possible involvement of DExD/H box helicase 60 in synovial inflammation of rheumatoid arthritis: role of Toll-like receptor 3 signaling
Get PDF弘前大学博士（医学）掲載誌：Molecular Biology Reports 2024 Jan 18;51(1):13
Heat Shock Protein SSA1 Enriched in Hypoxic Secretome of Candida albicans Exerts an Immunomodulatory Effect via Regulating Macrophage Function
Get PDFCandida albicans is an opportunistic pathogenic yeast that can survive in both normoxic and hypoxic environments. The involvement of C. albicans secretome on host biological processes has been demonstrated. However, the immunoregulatory function of C. albicans secretome released under hypoxic condition remains unclear. This study demonstrated the differences in cytokine responses and protein profiles between secretomes prepared under normoxic and hypoxic conditions. Furthermore, the immunoregulatory effects of heat shock protein SSA1(Ssa1), a protein candidate enriched in the hypoxic secretome, were investigated. Stimulation of mouse bone marrow-derived macrophages (BMMs) with Ssa1 resulted in the significant production of interleukin (IL)-10, IL-6, and tumor necrosis factor (TNF)-α as well as the significant expression of M2b macrophage markers (CD86, CD274 and tumor necrosis factor superfamily member 14), suggesting that C. albicans Ssa1 may promote macrophage polarization towards an M2b-like phenotype. Proteomic analysis of Ssa1- treated BMMs also revealed that Ssa1 reduced inflammation-related factors (IL-18-binding protein, IL-1 receptor antagonist protein, OX-2 membrane glycoprotein and cis-aconitate decarboxylase) and enhanced the proteins involved in anti-inflammatory response (CMRF35-like molecule 3 and macrophage colony-stimulating factor 1 receptor). Based on these results, we investigated the effect of Ssa1 on C. albicans infection and showed that Ssa1 inhibited the uptake of C. albicans by BMMs. Taken together, our results suggest that C. albicans alters its secretome, particularly by promoting the release of Ssa1, to modulate host immune response and survive under hypoxic conditions.弘前大学博士（医学）Author: Wei Teng, Phawinee Subsomwong, Kouji Narita, Akio Nakane, Krisana AsanoCitation: Cells;13(2):127, 2024 Jan 1
Immunohistochemical and molecular profiles of heterogeneous components of metaplastic breast cancer: a squamous cell carcinomatous component was distinct from a spindle cell carcinomatous component
Get PDFMetaplastic breast carcinoma (MBC), a category of breast cancer, includes different histological types, which are occasionally mixed and heterogeneous. Considering the heterogeneity of cancer cells in a tumour mass has become highly significant, not only from a biological aspect but also for clinical management of recurrence. This study aimed to analyse the immunohistochemical and molecular profiles of each MBC component of a tumour mass. Twenty-five MBC tumours were histologically evaluated, and the most frequent MBC component (c) was squamous cell carcinoma (SCC), followed by spindle cell carcinoma (SpCC). A total of 69 components of MBC and non-MBC in formalin-fixed paraffin-embedded sections were examined for 7 markers by immunohistochemistry. SCC(c) were significantly PTEN negative and CK14 positive, and SpCC(c) were significantly E-cadherin negative and vimentin positive. Multivariate analyses revealed that immunohistochemical profiles of normal/intraductal (IC)(c), no special type (NST)(c), and MBC(c) differed; moreover, SCC(c) and SpCC(c) were distinctly grouped. PTEN gene mutation was detected only in SCC(c) (2/7), but not in SpCC(c). Next-generation sequence analyses for 2 cases with tumours containing SCC(c) demonstrated that PTEN gene mutation increased progressively from IC(c) to NST(c) to SCC(c). In conclusion, the immunohistochemical and molecular profiles of the SCC(c) of MBC are distinct from those of the SpCC(c).弘前大学博士（医学）Author: Takahiro Suzuki, Yoko Nakanishi, Tomoyuki Tanino, Haruna Nishimaki‑Watanabe, Hiroko Kobayashi, Sumie Ohni, Xiaoyan Tang, · Kenichi Hakamada, Shinobu MasudaCitation: Discover Oncology 15(1):95, 2024 Apr
High-signal venous sinuses on MR angiography: discrimination between reversal of venous flow and arteriovenous shunting using arterial spin labeling
Get PDF弘前大学博士（医学）掲載誌：Neuroradiology 2021 Jun;63(6):889-89
A Logistic Regression Model for Predicting the Risk of Subsequent Surgery among Patients with Newly Diagnosed Crohn’s Disease Using a Brute Force Method
Get PDF弘前大学博士（医学）掲載誌：Diagnostics 2023 Dec 3;13(23):358
Association between injury severity scores and clinical outcomes in patients with traumatic spinal injury in an aging Japanese society
Get PDF弘前大学博士（医学）掲載誌：Medicine 2023 Sep 29;102(39):e3536
Utilization of and barriers to a telemedicine system at a rural general hospital in Japan: a mixed methods study
Get PDF弘前大学博士（医学）掲載誌：Journal of Rural Medicine 2023 Oct;18(4):226-232, Epub 2023 Oct 1
Reappraisal of telesurgery in the era of high-speed, high-bandwidth, secure communications: Evaluation of surgical performance in local and remote environments
Get PDF弘前大学博士（医学）掲載誌：Annals of Gastroenterological Surgery 2022 Aug 12;7(1):167-174, eCollection 2023 Jan