Defence Life Science Journal
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    Composting of Agricultural Waste Residues by Effective Streptomyces rameusKAC3 and Streptomyces mutabilis KAC6 Isolates for the Mass Production of Vermicompost

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    Agricultural sector after yield harvest remains plant parts huge environmental pollution issues because not suitable technology makes composting process. In this point of view, present investigation rigorously scrutinizes the agricultural waste decomposition process facilitated successfully completion in 40 days duration compost maturation by use effective of Streptomyces rameus KAC3 and Streptomyces mutabilis KAC6 to increase nutrient availability. Different agricultural crop waste is being used in this composting technology process which stem, root and leaves from multiple crops. The parameters reveled which include temperature, pH, moisture content, microbial populations, and nutrient analysis at various composting maturation stages, Based on findings emerge during degradation process the temperature fluctuation days 10th (34.6±0.06), 20th (49.37±0.14), 30th (62.4±0.06) and end of 40th day (32.7±0.15) and alongside pH elevations increases ranged from 5.2 to 6.53. The 30th day marks the zenith of microbial populations reaching 71.23±0.2 (109 CFU) and 9.28±0.09 (109 CFU), respectively. Furthermore, examination of vermicompost nutrient availability values reveals its superior profile compared to effective microorganism (EM) compost, with organic carbon (12.17%), nitrogen (1.24%), phosphorus (1.39%), potassium (0.76%). Finally, the research concluded that the plant nutrient availability was enhanced through incorporation of effective actinomycetes KAC3 and KAC6 to convert Agro-waste material to mature compost for further processing of vermicompost

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    Seabuckthorn (Hippophae Rhamnoides L.): An Economically Important Shrub of Cold-Temperate Regions

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    Seabuckthorn (Hippophae rhamnoides L.) is one of the ancient flowering plant species. In India, Seabuckthorn is found naturally growing in cold arid areas of Ladakh, Lahaul and Spiti, Kinnaur, Pangi, parts of Kumaun and Garhwal region and certain areas in northeastern hills of Indian Himalayan region. It can withstand extreme temperature from −40 °C to +40 °C with limited soil nutrients. It has gained global attention for its health benefits, primarily, rich metabolic profile; vitamin C being the most significant nutrient. It is a valuable source for developing and commercialisation of products such as fruit juice, tea, oil, wine and cosmetics. The primary application includes nutritional, pharmaceutical, food and feed, cosmetology and ecological restoration. This study explores the morphology, cultivation, and economic potential of Seabuckthorn, addressing its applications, toxicity, and safety concerns, and paving the way for further research. However, the species has yet to receive focused attention from scientists and conservationists with respect to its cultivation and ecological significance

    Seabuckthorn Technologies for Development of Value Chain and Environmental Conservation in Himalayas

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    Seabuckthorn (Hippophae spp.), an important high value medicinal plant found in cold areas of Indian Himalayan Region (IHR). In India, it is reported in Chamoli district of Uttarakhand, cold regions of Leh and Ladakh, Kinuar district of Himachal Pradesh, Sikkim and in Dibang valley in Arunachal Pradesh. Seabuckthorn considered as wonder berry from the Himalayas is known as nature’s most balanced fruit is a highly versatile high-altitude species having a wide range of ecological, medicinal, and economic uses. This hardy species can grow well in the extreme alpine and subalpine environments of the Himalayas, and have the tendency to withstanding at −40°C temperatures, and plays a vital role in nitrogen fixation and soil erosion control through its deep fibrous roots

    Hemp (Cannabis sativa) - An Underexploited but Promising Oilseed with Multifarious Properties and Applications: A Review

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    Hemp commonly known as bhang belongs to the Cannabaceae family. Due to the psychoactive effect, it is often stigmatised; however, recently the Government of Uttarakhand has legalised the cultivation of hemp in the state with <0.3 % 9-tetrahydrocannabinol levels. Hence this review provides comprehensive details on how the whole plant of hemp can be potentially used in various industries. Researchers identify hemp as a rich source of nutrients. Specifically, hemp seeds are being celebrated due to the presence of fatty acids, amino acids, and antioxidants. The presence of these nutrients makes hemp seeds a valuable ingredient in functional foods. Additionally, several studies recognised hemp for several health benefits viz., managing neurological disorders, preventing several cancers, easing skin problems and promoting better mental health. Alongside the health benefits, the hemp plant is recognised to have diverse applications in other industries such as cosmetics, textiles and construction. However, due to the legal constraints and local stigma large and nation-wide cultivation of hemp is difficult but extensive research into the cultivation and processing of hemp can help to leverage this plant for the associated benefits

    Pathophysiology of Dermatophytes and Potent Phytochemical Sources as Possible Applications against Trichophyton Spp.

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    Trichophyton infections are typically restricted within the epithelial keratinized layer of the skin and therefore grouped among the dermatophytoses. Different species of Trichophyton viz., T. mentagrophytes, T. rubrum and T. violaceum are the important causal agents for the dermatophytic disorders. Azole drugs are extensively employed in the therapeutic practice to treat dermatomycosis. Use of synthetic drugs can cause a number of side effects and may induce drug resistance to the patients. Therefore, plant extracts have been widely investigated as alternative for chemical drugs to solve this problem. Literature data revealed that specific plants showed inhibiting effect against particular species of Trichophyton because of their different phytochemical contents. This study is carried out to document different plant species for their antifungal activity against various species of Trichophyton. Total of 107 plant species belonging to 50 families have been gathered for their antifungal activity against nine species of Trichophyton species viz., T. asahii, T. erinacei, T. inkin, T. ovoides, T. schoenleinii, T. longifusus, T. soudanense, T. tonsurans and T. verrucosum

    Eco-Friendly Synthesis of Silver Nanoparticles from Trans-Himalayan Herbs: A Comparative Study of Biological Activities

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    The current study aims to synthesise silver nanoparticles (AgNPs) using three medicinal plants-Inula racemosa, Rhodiola imbricata, and Ephedra gerardiana-native to the Trans-Himalayan region. These plants were specifically selected due to their well-documented ethnomedicinal uses and rich phytochemical profiles, particularly in phenolic compounds. The study aims to harness their bioactive potential for the development of eco-friendly, cost-effective nanomaterials with therapeutic applications. Synthesised AgNPs were characterised via UV–VIS spectrophotometer, SEM, FTIR, DLS, TEM and EDX analysis. The absorption peaks at 410 nm (I. racemosa), 440 nm (R. imbricata), and 420 nm (E. gerardiana), confirming nanoparticle formation via surface plasmon resonance. Phytochemical analysis highlighted phenolics as key agents in nanoparticle reduction and capping. Although the AgNPs showed lower antioxidant activity than the crude extracts but exhibited superior antibacterial efficacy against multidrug-resistant bacterial strains, outperforming the standard antibiotic Ampicillin. Cytotoxicity evaluation against MCF-7 breast cancer cells revealed that I. racemosa root extract and its AgNPs had the highest anticancer activity, with IC₅₀ values of 64.93 μg/mL and 55.88 μg/mL, respectively. This research proposes a sustainable approach to develop alternative antibacterial and anticancer agents, potentially addressing the rising global challenge of antibiotic resistance and cancer burden through plant-based nanotechnology

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    Characterisation, Synthesis, and Antimicrobial Activities of Silver Nanoparticles Formed via Green Synthesis Approach using Aqueous Extract derived from the Bark of Mangifera indica: A Sustainable Resource

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    Many research opportunities have arisen as a result of the green synthesis method for nanoparticle creation and the rapid development and breakthroughs in nanotechnology. This study covers the one-step synthesis of silver nanoparticles (SNPs) from Mangifera indica bark aqueous extracts. Bark extract is combined with AgNO3 to perform green synthesis of SNP. The synthesised SNPs were characterised by a color change from light yellow to deep brown, as well as UV-VIS spectrophotometry in the 300-700 nm area. Further characterisations were carried out using Fourier transform infrared spectroscopy (FTIR) and X-Ray Diffraction (XRD) studies, followed by Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray spectroscopy (EDX) analysis. Phytochemical examination of crude bark extract revealed the existence of secondary metabolites alkaloids, flavonoids, phenolics, tannins, and saponins but no glycosides and starch were present. The antimicrobial activity (AMA) of Bark SNP (BSNP) towards gram-positive bacteria [Staphylococcus Aureus (SA) & Streptococcus Pyogenes (SP)], gram-negative bacteria [E. coli (EC) & Pseudomonas aeruginosa (PA)], and Candida species were determined using the disc diffusion technique. A single-factor ANOVA was used for performing the statistical analysis, and p-values < 0.05 designated statistical significance. The green synthesised SNPs were spherical and crystal size ranged between 10 and 12 nm on average. In UV-vis spectroscopy, the absorption spectra peaked at 460 nm. It was discovered that the SNPs’ antibacterial activity was stronger than their antifungal activities. Compared to commercial medicines, the BSNP exhibits a reasonable inhibitory effect. The purpose of the current study was to build a new, affordable, ecologically safe process for a plant-mediated green approach for SNP synthesis and evaluate its antimicrobial activity for sustainable resources

    Phytochemical Evaluation and GC-MS Analysis of Leaf Extract of Manihot Esculenta Crantz

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    Plants contain a variety of bioactive compounds which makes them an important foundation of pharmaceutical research. Manihot esculenta Crantz is a perennial shrub with medicinal prospects. The current research was conducted to qualitatively estimate the phytochemicals and Gas Chromatography-Mass Spectroscopy analysis of its methanol extract to identify the compounds present. The qualitative phytochemical estimation was done using standard tests. The GC-MS study of the methanolic extract was done following standard protocol. The phytochemical analysis detected alkaloids, flavonoids, saponins, phenols, steroids, tannins, carbohydrates, glycosides, triterpenoids, ketones, and proteins. A total of 24 bioactive compounds were revealed through GC-MS analysis, including hentriacontane, tetrapentacontane, 1,54-dibromo-, dotriacontyl isobutyl ether, 3-methyl-2-(2 oxopropyl) furan, 4-dodecene-6,8,10-triyn-3-one, (e)-, z,z-6,27-hexatriactontadien-2-one, cholest-5-en-3-ol (3.beta.)-, and propanoate. The findings of this study support its traditional use in management of diabetes. Nevertheless, more research is needed to determine the specific mechanism of action and to characterise the active chemicals found in the extract

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