International Journal of Research in AYUSH and Pharmaceutical Sciences (IJRAPS)
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DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL AND LORNOXICAM IN BULK AND PHARMACEUTICAL DOSAGE FORM
Get PDFA simple, rapid, accurate and precise isocratic reversed phase high performance liquid chromatographic method has been developed and validated for simultaneous estimation of Paracetamol and Lornoxicam in tablet dosage form. The chromatographic separation was carried out on Zorbax C18 column (150 mm x 4.6 mm I.D., 5 µm particle size) with a mixture of 20 mM ammonium acetate pH 3.2 buffer and acetonitrile in the ratio of 60:40 v/v as a mobile phase at a flow rate of 1.0 mL/min. UV detection was performed at 265 nm. The retention times were 2.74 minutes and 5.36 minutes for Paracetamol and Lornoxicam respectively. Calibration plots were linear (r2=0.999 for both Paracetamol and Lornoxicam respectively) over the concentration range of 6.25-250 µg/mL for Paracetamol and 0.1-4 µg/mL for Lornoxicam. The method was validated for linearity, precision, accuracy, ruggedness and robustness. The proposed method was successfully used for simultaneous estimation of Paracetamol and Lornoxicam in tablet dosage form. Validation studies revealed that the proposed method is specific, rapid, reliable and reproducible. The high % recovery and low % RSD confirms the suitability of the proposed method for routine quality control analysis of Paracetamol and Lornoxicam in bulk and tablet dosage form
A STUDY TO ASSESS THE PREVALENCE OF MORBIDITY PROFILE AMONG WOMEN’S DURING PREGNANCY AT PRIMARY HEALTH CARE CENTRE IN THIRUMAZHASAI
Get PDFIn most developing countries, pregnancy and childbirth are accepted as normal events of life and it is not surprising that problems associated with pregnancy are also accepted. In developing countries interventions that are known to be effective in reducing maternal and perinatal mortality and morbidity are not universally provided. Objectives: Assess the prevalence of morbidity profile in selected demographic variables of antenatal mothers. Methodology: Descriptive research design was chosen to assess the prevalence of morbidity profile among women’s during pregnancy in primary health care center in Thirumazhasai. The study was conducted in primary health centre in thirumazhasai. In this study population refers to all the antenatal mothers attending the antenatal clinic in the primary health care centre in thirumazhasai. The sample size was 60 antenatal mothers. Non-probability, convenient sampling technique was used. Result: Frequency and percentage distribution of the demographic variables among antenatal mothers. It shows that out of 60 samples, among 12 (20%) were in the age group of below 19 years, 39 (65%)were in the age group of 20-30 years, 9 (15%) were in the age group of above 30 years, 32 (53%) were primi gravida, 32 (53%) were got marriage below 19 years. Assess the prevalence of morbidity profile among women’s during pregnancy period. It shows that the prevalence of morbidity profile among women’s during pregnancy among 60 samples has an obstetric problems, 44 (73%) has anemia, 20 (33%) has increased blood pressure, 17 (28%) has diabetes mellitus, 3 (5%) has seizure during pregnancy, 3 (5%) has cord prolapse, 3 (5%) has premature labor, 9 (15%) has hydraminos, 2 (3%) has ectopic pregnancy. It shows the prevalence of morbidity profile among women’s during pregnancy among 60 samples has an non-obstetric problems, 52 (87%) has tenderness and swollen of breast, 59 (98%) has nausea, 59 (98%) has vomiting, 59 (98%) has fatigue and giddiness, 57 (95%) has a headache, 59 (98%) has increased urination
PREBIOTIC POTENTIAL OF STARCH OF TINOSPORA CORDIFOLIA, A COMPONENT OF SOMAVIT®: AN IN VITRO STUDY ON GROWTH MODULATION OF BIFIDOBACTERIUM AND LACTOBACILLUS STRAINS
Get PDFPrebiotic is a substrate that is selectively utilized by host microorganisms conferring a health benefit. The present study aimed to investigate the prebiotic potential of starch of Tinospora cordifolia (5% w/v) based on in vitro growth stimulation of Lactobacillus plantarum and Bifidobacterium bifidum, using individual strains and consortia. Bacterial cultures were incubated under anaerobic conditions with starch of Tinospora cordifolia (test) and with distilled water as the control. At various contact time points (0, 1, 48 and 96 hours), small aliquots were sampled. The organisms were plated on specific growth media under anaerobic conditions for Bifidobacterium and microaerophilic for Lactobacillus, and total viable count was determined by standard plate count. Starch of Tinospora cordifolia promoted growth of both bacterial strains, singly and in consortia. The mean log colony forming units (CFU) ± standard deviation of Lactobacillus in test was significantly higher in comparison to control (8.09±0.07 vs 7.06±0.03, p<0.05) at 48 hours incubation, thereafter the growth of Lactobacillus plateaued through 96 hours. CFUs for Bifidobacterium in test were 8.52±0.28 at 48 hours and 8.74±0.14 at 96 hours incubation, both significantly higher (p<0.005) when compared to control. Bifidobacterium in test exhibited 90-fold relative increase in growth when compared to control. Lactobacillus being more robust showed an immediate increase in growth, while Bifidobacterium demonstrated a delayed but sustained growth, which extended over a period of time. These findings suggest that Starch of Tinospora cordifolia may enhance the gastrointestinal health of the host through modulation of overall composition of gut microbiota
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPLC METHOD FOR THE DETERMINATION OF ULIPRISTAL ACETATE IN PHARMACEUTICAL DOSAGE FORM
Get PDFA simple, novel, precise and accurate stability indicating RP-HPLC method was developed and validated for the estimation of Ulipristal Acetate in pharmaceutical dosage form. A Phenoxneome C18 (150 mm x 4.6 mm, 5 µm) column was used as stationary phase with mobile phase consisting of 0.1% ortho phosphoric acid and acetonitrile in the ratio of 50:50 v/v (pH was adjusted to 4.0 with triethyl amine). The flow rate was maintained at 1.0 mL/min and effluents were monitored at 223 nm. The retention time was 1.895 min. The linearity of the method was observed in the concentration range of 20-100 µg/mL with correlation coefficient of 0.999. The method developed was validated for linearity, precision, accuracy, system suitability and forced degradation studies like acidic, alkaline, oxidative and neutral stress conditions were performed as per ICH guidelines. The results obtained in the study were within the acceptable limits and hence this method can be used for the estimation of Ulipristal Acetate in pharmaceutical dosage form
Evaluation of Antibacterial Activity of Deepika Rasa
Get PDFNow a day’s infectious diseases are posing problem for human beings. In modern era for the development of Ayurvedic medicine with the proof scientifically and use of new search antibiotics to be increase, So we studied for pharmaceutical & diagnostic parameter standardization of Deepika rasa. Deepika rasa is not popular traditional medicine so an attempt has been made to develop a safe & less expensive antimicrobial drug.
Deepika rasa formulations are selected for the present study from Rasa rattan samuccaya (U.K.A12). Deepika rasa was indicated as “Sarva Jwarhar Vinashanam”. The antibacterial activity of the Deepika rasa was tested against 5 pathogenic bacterial strain Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhi. These pathogens are very common for fever.
In order to study antibacterial action of Deepika rasa in vitro well diffusion method. During this study Deepika rasa was trailed with bacterial at different concentration. To correlate the result control solution were prepared by streptomycin. Experimental group were compared with control group and observation were noted.
The encouraging results obtained from anti microbial study of Deepika rasa. Deepika rasa was highly significant for some pathogen with the different concentration & moderately significant for some pathogen with the different concentration & no significant for some pathogen with the different concentration. So an attempt was made to find a safe and effective Ayurvedic medicine
CLINICAL EVALUATION OF UNANI DRUGS MAJOON MUQAWWI RAHIM IN THE SAYALAN AL RAHIM (SIBYANI) PRE PUBERTAL VAGINAL DISCHARGE: A PRELIMINARY STUDY
Get PDFThe pre-pubertal vulvo vaginal discharge (sayalan al rahimsibyani) is increasing slowly but steadily in young girls has been a well-known disease among young women since ages. Now a day’s almost all Unani physicians at from Hippocrates 360 BC down to Shiekh Abu Ali Sina 910 AD Ibn –ul-Nafees, Ibn e Zuhr and Indian origin physicians like Hakeem Shareef Khan, Hakeem Akbar Arzani, Hakeem Mohd Azam Khan and Hakeem Abdul Aziz have mentioned the disease in there treatise and suggested single as well as compound drugs for internal and external use, The study has been conducted successfully by the Central Research Institute of Unani Medicine Lucknow, to evaluate the efficacy and safety of Unani drugs in the treatment of the disease. The pre pubertal vaginal discharge is not a new phenomenon in young girls, but due to the social stigmait is discussed in Indian society. Some studies suggest that various internal and external factors are causing the disease but to the best of over knowledge only a few studies have been conducted in the Indian sub-continent hence the Central Research Institute of Unani Medicine, ministry of AYUSH Govt. of India took upon itself the responsibility and conducted a clinical trial on 22 young girls with consent of their parents and Majoon muqawwi rahim an Unani pharmacopeial formulation was administrated orally in semi solid form and the results are so encouraging that CCRUM may plan a bigger study in large scale and lead the country in the treatment of PPV through herbal Unani formulation and provide relief the suffering young girls
STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METFORMIN AND GLIPIZIDE
Get PDFA novel, precise and accurate stability indicating RP-HPLC method was developed and validated for the simultaneous estimation of Metformin and Glipizide in combined pharmaceutical dosage form. Chromatographic separation was achieved on Microsorb-MV C18 column (250 × 4.6 mm, 5 μm) with UV detection at 257 nm. The mobile phase consists of acetate buffer (pH 4.0) and acetonitrile in the ratio of 60:40 v/v and at a flow rate of 1.0 mL/min. The method was linear over the concentration range of 60-140 μg/mL for Metformin and 10-50 μg/mL for Glipizide. The retention times for Metformin and Glipizide were found to be 2.434 min and 5.710 min respectively. The mean percentage recoveries of Metformin and Glipizide were found to be 100.42% and 100.39% respectively. The method was validated and was successfully employed for the routine quantitative analysis of pharmaceutical formulations containing Metformin and Glipizide in combined pharmaceutical formulation
ANALYTICAL STANDARDIZATION OF RAJATA BHASMA
Get PDFBhasma Kalpana is a unique part of Ayurvedic Rasa shastra. It is a method of converting metals into metallic Bhasma. Metallic Bhasmas are well known for its quick effectiveness, smaller dose and a long shelf life. However if these Bhasmas are not well prepared and analyzed they can be toxic to human body. Therefore Bhasma Pariksha is given in Ayurveda to confirm the well prepared metallic Bhasma, but in this era we need to analyze the Bhasmas on modern parameters too to make it acceptable globally. So in this study prepared Rajata (Silver) Bhasma is analyzed on various parameters i.e. Ayuvedic parameters i.e., Varitaratwa, Rekhapurnatwa, Slakshantwa and Laghutwa and some modern parameters like, pH, Particle size, Zeta Potential, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy dispersive X-ray spectroscopy, Fourier Transform Infra red Spectroscopy (FTIR), U V – Spectroscopy.
Results: pH value is 5.6, UV- spectroscopy showed maximum absorption at 302nm, FT-IR of Rajata bhasma reveals the presence of C-H (alkyl), C-N (Aliphatic amine) groups at different wavelengths, Mean Particle size of Rajata bhasma is 2.7nm, Zeta Potential analysis reveals -19.3 mV, X-RD of Rajata bhasma reaveals the major peaks of HgS (Cinnabar), Ag2S (Silver Sulphide), SEM analysis of Rajata bhasma showed small crystalline particles at 5KX & 10KX magnifications, EDX analysis of Rajata bhasma confirmed the presence of elements viz., Sulphur 20.34%, Oxygen 7.43%, Silver 26.72%, Mercury 45.51% by weight
BASIC CONCEPT OF YOGA AND ITS HEALTH BENEFITS- A SHORT REVIEW
Get PDFYoga is a group of physical, mental, and spiritual practices which has its root in ancient India. Yoga is a complete science by itself being a holistic and very scientific background. These days everyone is suffering from some sort of lifestyle disorders, anxiety, stress, depression etc. and almost all streams of medicines have their limitations for these diseases. Ancient Acharyas have given the world a gift called Yoga which is proving extremely beneficial from children to adults to get relief from life style disorders. Yoga science is magnificently described in many ancient treatises like Vedas, Bhagavdgita, Yogavashishta, Patanjal Yogasutra, Gherand Samhita. Yoga is accomplished by Asthanga known as Yama, Niyama, Asana, Pranayama, Pratyahara, Dharana, Dhyana and Samadhi. Now-a-days, millions and millions of people across the globe have benefitted by the practice of Yoga which has been preserved and promoted by the great eminent Yoga Masters from ancient time to this date
PREVENTIVE CARDIOLOGY IN AYURVEDA W.S.R TO NON-SUPPRESSIBLE URGES
Get PDFThere is a wide range of risk factors which affect cardiovascular conditions. Cardiovascular diseases comprise one of the most serious disorders in developed nations and are rapidly rising in developing countries. Ayurveda explains, cessation of natural urges as one of the major causes of CVD. In due course of time, suppression of non-suppressible urges, may be due to socially unacceptable behavior, work load or lifestyle problems, usually develops in complications related with heart which are frequently ignored. These non-suppressible urges include Vata (flatus), Vit (faeces), Mootra (urine), Kshawathu (sneeze), Trishna (thirst), Kshudha (hunger), Nidra (sleep), Kasa (cough), Shrama Janit Shwas (breathing out of exertion), Jrimbha (Yawning), Ashru (tears), Chhardi (vomit), Retas (semen) and Udgara (burp). The pathogenesis of cardiovascular disorders can be explained on the basis of Vata vitiation due to obstruction in natural course of Vata resulting in its upward or unnatural course of movement. For treating these conditions, Vata Anulomak food and medicines should be used