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Interactions between human norovirus virus-like particles and bacteria: Impact on thermal resistance
No full textInternational audienceHuman norovirus, a leading cause of foodborne gastroenteritis, is known for the virus's resistance to inactivation and environmental persistence. It interacts with histo-blood group antigens, its only known ligands. Due to the lack of an efficient cell culture system, noninfectious virus-like particles (VLPs) are used for research.This study aimed to identify virus-microbe pairs, characterize their interactions, and assess their effects on bacterial heat resistance. VLPs of genotypes GII.4 and GII.17 were tested against 77 microbial strains, including 44 pathogenic bacteria, 4 fermentative bacteria, and 6 yeasts.Specific interactions were observed between GII.17 VLPs and Staphylococcus aureus or Enterobacter cloacae, mediated by surface sugar-dependent mechanisms confirmed by sodium periodate treatment. No binding was detected with GII.4 VLPs. Competition assays suggested that histo-blood group antigen-like structures were not involved. Atomic force microscopy revealed direct attachment of VLPs within the exopolysaccharide matrix of E. cloacae.Environmental factors significantly affected binding efficiency. It was influenced by buffer composition, increasing in citrate buffer, and by oxygen availability, being abolished under anaerobic conditions. Furthermore, attached particles enhanced bacterial heat resistance. These findings provide new insight into norovirus persistence and virulence, particularly for genotype GII.17, which, though less prevalent, exhibits stronger binding affinity
Mobiliser les interactions plante-sol en sélection : quels traits et quelles méthodes considérer pour sélectionner des variétés mieux adaptées à des systèmes de culture agroécologiques ?
No full textLivre blancInternational audiencePlantAlliance publie un livre blanc intitulé « Mobiliser les interactions plante-sol en sélection : quels traits et quelles méthodes considérer pour sélectionner des variétés mieux adaptées à des systèmes de culture agroécologiques ? ». Ce Livre Blanc est le fruit des réflexions menées au sein du Groupe de Travail ‘Agronomie et Génétique’ du consortium, lancé par Mylène Durand-Tardif, co-animé par Jan Gielen (Innolea), Baptiste Soenen (RAGT Semences) et Charles-Eric Durel (INRAE-PlantAlliance), avec l’appui d’Edith Le Cadre (L’Institut Agro Rennes-Angers) au départ. Après des éléments de contexte et un travail sur les traits des systèmes racinaires pouvant apporter des services écosystémiques, ce Livre Blanc recense les projets de recherche récents ou en cours qui traitent des interactions plante-sol et les méthodes et outils permettant de mesurer ou caractériser ces traits. Il aborde enfin les questions de mise en œuvre concrète et de mobilisation de ces traits en sélection en identifiant les verrous et les manques d’information, les nouvelles approches envisagées pour cibler directement des traits racinaires, les questions d’envirotypage, les nouveaux objectifs de sélection dans une perspectives d’agroécologie et d’adaptation ou d’atténuation face au changement climatique, et les questions de recherche ou développements opérationnels prioritaires à développer
Déclaration et vérification des créances. Omission d'un élément essentiel dans l'avis du jugement d'ouverture inséré au BODACC. Conséquences
No full textInternational audience(Com. 2 juill. 2025, no 24-11.217, F-B, URSSAF c/ Sté BT Zimat et a., D. 2025. 1206 ; ibid. 1593, obs. P. Cagnoli et F.-X. Lucas
Differences in mean daily intake of macronutrients, caffeine and water by level of mental morbidity
No full textInternational audienceWe investigated macronutrient, caffeine and water intake by mental morbidity level, focusing on pure and comorbid anxiety, insomnia, and eating disorders (ED). This descriptive, cross-sectional study used 2013-2017 data from the NutriNet-Sant & eacute; cohort. Diet was assessed with >= 3 24-hour records; mean daily intake of total energy and % contribution of carbohydrates, proteins, and lipids to total energy; total, simple, complex carbohydrates; total, animal, vegetable protein; total lipids, SFA, MUFA, PUFA; cholesterol; caffeine and water was analyzed. Data were weighted using the 2016 French Census, and ANOVA was performed (N = 23,965). Overall, men presenting all 3 mental disorders had the highest % carbohydrates and the lowest % proteins and lipids. The multimorbidity group had the highest obesity rates and caffeine intake across sex; water intake had no dose-response association with mental morbidity. The findings could help generate hypotheses regarding dietary exposures that might play a role in mental multimorbidity risk
La prescription de l'action en exequatur en France d'une sentence arbitrale rendue à l'étranger
No full textInternational audience(Versailles, 10 déc. 2024, no 23/03647, X. et Citygroup Global Markets Inc. C. Y., Rev. arb. 2025. 211, note S. Bollée, obs. L. d'Avout, D. 2025. 1710, Panorama Droit du commerce international ; JDI 2025. Comm. 11, obs. L. Larribère
Preparation of hybrid SPIONs and nanodiamonds in view of neurodegenerative diseases treatments
No full textInternational audienceSuperparamagnetic iron oxide nanoparticles (SPIONs) are widely used in biomedical applications due to their unique magnetic and physicochemical properties. Surface stabilization and functionalization further expand their applicability in biological environments and help overcome the challenges of achieving targeted therapy. Here, nanodiamonds (NDs) decorated with SPIONs were developed for a dual therapy approach combining magnetic and optical properties: NDs offer excellent properties for optical imaging while SPIONs serve as effective contrast agents. In the present study, amino-PEGylated SPIONs were covalently linked to carboxylated NDs via carbodiimide conjugation, to enhance the colloidal stability of the nanoparticles (NPs) and improve their biocompatibility and stealth properties. The functionalization process was evaluated using various analytical techniques, including TGA, XPS, FTIR, DLS, and zeta potential measurements, which confirmed and validated successful functionalization in each consecutive step. Given that organelle dysfunction plays a critical role in neurodegeneration, and in an effort to specifically target peroxisomes, the serine-lysine-leucine (SKL) peptide was thus anchored on ND-PEG-SPIONs surface with the remaining free amines. To further assess the biological response of these nanohybrids, their colloidal stability was compared in different biological media. Strikingly, based on the in vitro data obtained from BV-2 cells, we demonstrate that the nanoparticles accumulate intracellularly without inducing cell death or mitochondrial dysfunction, a result that fulfills a crucial prerequisite for their future application in targeted therapeutic delivery of antioxidant molecules to peroxisomes. This work thus paves the way for the development of in pexotherapy, opening new avenues for the treatment of neurodegenerative diseases associated with peroxisomal dysfunction
Un rôle de genre en faveur d’une éducation physique « féminine », Jacqueline Roger
No full textInternational audienc
Hecke cycles on moduli of vector bundles and orbital degeneracy loci
No full textInternational audienceGiven a smooth genus two curve C, the moduli space SU_C (3) of rank three semistable vector bundles on C with trivial determinant is a double cover in P^8 branched over a sextic hypersurface, whose projective dual is the famous Coble cubic, the unique cubic hypersurface that is singular along the Jacobian of C. In this paper we continue our exploration of the connections of such moduli spaces with the representation theory of GL_9, initiated in [GSW13] and pursued in [GS15, RS18, RS19, BMT21]. Starting from a general trivector v in ∧^3 C^9 , we construct a Fano manifold D_Z_10 (v) in G(3, 9) as a so-called orbital degeneracy locus, and we prove that it defines a family of Hecke lines in SU_C (3). We deduce that D_Z_10 (v) is isomorphic to the odd moduli space SU_C (3, O_C (c)) of rank three stable vector bundles on C with fixed effective determinant of degree one. We deduce that the intersection of D_Z_10 (v) with a general translate of G(3, 7) in G(3, 9) is a K3 surface of genus 19
On design, analysis, and hybrid manufacturing of microstructured blade-like geometries
No full textInternational audienceWith the evolution of new manufacturing technologies such as multi-material 3D printing, one can think of new type of objects that consist of considerably less, yet heterogeneous, material, consequently being porous, lighter and cheaper, while having the very same functionality as the original object when manufactured from one single solid material. We aim at questioning five decades of traditional paradigms in geometric CAD and focus at new generation of CAD objects that are not solid, but contain heterogeneous free-form internal microstructures. We propose a unified manufacturing pipeline that involves all stages, namely design, optimization, manufacturing, and inspection of microstructured free-form geometries. We demonstrate our pipeline on an industrial test case of a blisk blade that sustains the desired pressure limits, yet requires significantly less material when compared to the solid counterpart
Efficacy, safety, pharmacokinetics, immunogenicity, and serum neutralizing activity of AZD7442 (tixagevimab-cilgavimab) in patients hospitalized with COVID-19: long-term results from the DisCoVeRy trial
No full textInternational audienceObjectives: To report long-term clinical efficacy, safety, pharmacokinetics, immunogenicity and seroneutralization results of AZD7442 (monoclonal antibodies tixagevimab-cilgavimab) in patients hospitalized with COVID-19.Methods: In this phase 3, double-blind, randomized, multicentre trial, hospitalized adults with PCR-confirmed SARS-CoV-2 infection were randomly assigned 1:1 to receive AZD7442 or placebo, and followed-up until day 456, with repeated blood sample collections until day 365. Clinical endpoints included clinical status, mortality, rehospitalization, SARS-CoV-2 reinfection, and adverse events. Antidrug antibodies and serum drug concentrations were measured. Analyses were performed on the modified intention-to-treat (mITT) populations, defined as participants who actually received the intervention.Results: Between April 28, 2021, and June 23, 2022, 237 participants were randomly assigned to AZD7442 (n = 127) or placebo (n = 110), and 123 participants actually received AZD7442. Participants were infected with pre-Omicron variants in 58.8% (133/226) of cases, versus 33.2% (75/226) of Omicron BA1, BA2, or BA5, and 8% (18/226) missing data. There was no significant difference in the distribution of the 7-point ordinal scale between the AZD7442 and placebo groups, either on day 15 (primary endpoint) (OR = 0.93 [0.54-1.61], p 0.81), or any other time point. Significantly more rehospitalizations occurred between discharge and day 456 among participants who received AZD7442 in the global mITT population (OR = 2.04 [1.03-4.05], p 0.04), but not in the antigen-positive mITT population (OR = 1.78 [0.80-3.94], p 0.15). No significant differences were observed in mortality, SARS-CoV-2 reinfection, or adverse events. In the AZD7442 group, 12 of 87 participants (13.8%) had treatment-emergent antidrug antibodies versus 5 of 69 (7.2%) in the placebo group (OR = 2.02 [0.66-6.14], p 0.21). Serum drug concentrations were detectable up to day 365 for all sampled participants (35/35). Neutralizing antibody titres were significantly higher in the AZD7442 group up to day 180.Conclusions: AZD7442 did not demonstrate any clinical benefit and was safe up to 15 months. This study also provides valuable data on the pharmacokinetics, immunogenicity, and neutralizing activity of AZD7442 in patients hospitalized with COVID-19