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    The content and associated health risk assessment of toxic elements, micro-, and macrominerals in common carp, Wels catfish, and silver carp from the Danube River in Serbia

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    Three fish species (common carp, Wels catfish, and silver carp) were collected from three locations along the Danube River in Serbia, and fish meat was analyzed for the content of toxic elements, micro- and macrominerals. Silver carp had the highest lead (Pb), arsenic (As), and cadmium (Cd) content, while Wels catfish had the highest level of mercury (Hg). Moreover, metal pollution index (MPI) ranged from 0.1096 to 0.275 and among the fish, the silver carp had the highest MPI (from 0.21 to 0.28), indicating that it could be reliable bioindicator of river pollution. Maximum determined levels of As (0.1968 mg/kg of w.w.), Hg (0.175 mg/kg of w.w.), and Pb (0.0315 mg/kg of w.w.) did not exceed values prescribed by the European Commission Regulation, Codex Alimentarius Commission, Food and Agriculture Organization (FAO), and national regulations, but Cd in silver carp was at levels higher than prescribed (0.0808 mg/kg of w.w.). Furthermore, our study’s results showed that the target hazard quotient (THQ) of each analyzed element and hazard index (HI) were < 1, indicating that consumers would not be exposed to adverse health effects after consuming these fish species from the Danube River. Regarding target cancer risk (TR), for Pb this was below 10− 6, which was regarded as negligible, while TRs for As and Cr were between 10− 4 and 10− 6, which was regarded as acceptable. Moreover, since the studied fish had low values of health risk indexes, it could be concluded that fish meat did not pose a significant risk to human health

    Upotreba hladne vode koja se aktivira plazmom za dezinfekciju stočarskih objekata

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    Održavanje odgovarajućih higijenskih uslova u stočarskim objektima je ključno za obezbeđivanje zdravlja ljudi i životinja, ne zaboravljajući pritom na aspekt zaštite životne sredine. Jedan od novijih i istovremeno održivih pristupa u antimikrobnoj kontroli je upotreba hladne vode koja se aktivira plazmom. To je voda koja je tretirana hladnom plazmom – naprednom tehnologijom oksidacije, te se u njoj formiraju mnoge hemijske reakcije. Oni pokreću formiranje reaktivnih vrsta kiseonika i azota, smanjuju pH vrednost i povećavaju oksidoredukcioni potencijal, što su ključni faktori njene antimikrobne aktivnosti. Istraživanja su pokazala da je hladna voda aktivirana plazmom efikasna protiv širokog spektra patogena, što ukazuje na njenu moguću upotrebu u dezinfekciji stočnih objekata. Trenutno se u tu svrhu najčešće koriste hemijska dezinfekciona sredstva, koja zbog ostataka aktivnih jedinjenja nakon upotrebe mogu predstavljati rizik za životnu sredinu. Nasuprot tome, voda koja se aktivira hladnom plazmom ne sadrži dodata hemijska jedinjenja, što je čini održivom metodom antimikrobne kontrole.Zbornik radov

    Pravilan izbor insekticida kao faktor uspešne regulacije brojnosti muva

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    Mu ve (Muscidae) predstavljaju veliki zdravstveni, ekološki i ekonomski izazov. Njihova povećana brojnost na farmama negativno utiče na proizvodne rezultate i zdravstveni status životinja, kvalitet i zdravstvenu ispravnost namirnica koje se koriste u ishrani ljudi. Takođe, njihovo prisustvo u objektima za stanovanje ljudi, naročito u zonama farmi, utiče na kvalitet života i predstavlja zdravstveni rizik. U cilju prevencije prethodno navedenog neophodno je razviti efikasan pristup za suzbijanje muva na farmama i objektima za ljude, vodeći računa sa jedne strane o zdravlju farmskih životinja i zaposlenih na istim, kvalitetu i bezbednosti namirnica koje vode poreklo sa farmskih objekata, kao i kvalitetu života i zdravlju ljudi koji žive u stambenim objektima u neposrednoj blizini farmi. Danas se u skladu sa principima biosigurnosti, dobre farmske prakse i standardima koji definišu bezbednost hrane, razvijaju efikasne strategije suzbijanja muva u farmskim objektima, kao i objektima u kojima stanuju ljudi, a definisane su kao „Integrisani model upravljanja štetočinama (IPM)”, odnosno „Integrisani model upravljanja muvama (IFM)”. Navedeni model predstavlja holistički pristup, koji kombinuje različite metode i tehnike kako bi se postigla dugotrajna kontrola brojnosti populacije muva na farmskim objektima i prvenstveno objektima za boravak ljudi koji se nalaze u neposrednoj blizini istih. Cilj našeg rada je informisanje lokalnih veterinara sa integrisanim modelom upravljanja muvama (IFM), uz poseban osvrt na pravilan izbor insekticida, u cilju njihove efikasne primene u praksi.Zbornik radov

    Insekticidi uvedeni u praksu krajem dvadesetog i početkom dvadeset prvog veka

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    Otkrićem i uvođenjem u praksu raznih supstancija koji deluju štetno na insekte, poznatih pod imenom insekticidi, nesumnjivo su ostvareni veliki, a moglo bi se reći i grandiozni rezultati u zaštiti ljudi, životinja i biljaka od brojnih insekata. Kao što je poznato, njihovom primenom su iskorenjene određene zarazne bolesti, olakšano je suzbijanje i lečenje ektoparazitoza kod ljudi i životinja, te je unapređena i povećana poljoprivredna proizvodnja. U cilju povećanja poljoprivredne proizvodnje danas se u svetu na godišnjem nivou koristi velika količina (milioni tona) ovih sredstava. Ovo pre svega iz razloga što je prevladalo mišljenje da je poljoprivredna proizvodnja postala praktično nemoguća bez primene insekticida, ali i drugih pesticida. Upravo navedene činjenice, odnosno ovakva primena insekticida, koja je često i neracionalna, izaziva sve veću zabrinutost. Pored sve većeg razvoja rezistencije, a time i smanjenja efikasnosti insekticida, postoji sve veća zabrinutost i za zagađenje životne sredine. Nisu retki slučajevi svuda, pa i kod nas u našoj zemlji, da stradaju neciljni insekti, odnosno korisni insekti tj. pčele, ali i drugi organizmi u zemljištu i vodi. Cilj ovog rada je upravo da ukaže ne samo na moguće štete neracionalne primene insekticida, već i da se navedu neki od insekticida, koji su se pojavili na tržištu krajem prošlog i početkom ovog veka, te one biljnog porekla kao potencijalne zamene za insekticide, koji se trenutno koriste.Zbornik radov

    Nutritive value of inulin-collagen enriched low-fat emulsion type sausages

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    High intake of food reach in saturated fat and calories could lead to chronic non-infectious diseases in consumers. Meat products including cooked emulsified sausages are considered as such type of food, so different strategies for fat and energy reduction are widely investigated. Inulin-collagen suspension showed good properties to completely substitute fatty tissue in emulsified sausages. In this study, two groups of sausages were produced in order to evaluate their nutritional properties: control, containing 25% fatty tissue, and low-fat sausage produced without fatty tissue and enriched with inulin-collagen suspension instead. Low-fat sausage contained 0.9% fat, providing fat reduction of 96.5% compared to the control. The sum of fatty acids (SFA) and of trans-fatty acids was 0.31%. Caloric value was reduced for 78.1%, the share of proteins in total energy was 68.7%, and the share of saturated fatty acids in total energy was only 3.9%. Despite the addition of collagen and consequently significant increase in connective tissue proteins content, the quality requirements given by the regulations for the frankfurter type sausages were not disturbed, as connective tissue free meat protein content was 10.1% and connective tissue free meat proteins in meat proteins was 84.9%. The content of potentially harmful SFA was 96% and of n-6 fatty acids 98% lower than in the control group of products

    Pre-slaughter conditions and related effects on welfare and meat quality of slaughter horses: a mini review

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    In recent years, horsemeat has gained recognition as a valuable dietetic food due to its low caloric content, high-quality protein, rich vitamin and mineral composition and favourable fatty acid profile, making it a suitable alternative meat for individuals with anaemia, obesity, atherosclerosis, hypertension, organ diseases and compromised immunity. As with other livestock species, minimising pre-slaughter stress is essential for ensuring animal welfare and achieving high meat quality, as stress can result in bruising and/or other injuries. Therefore, the aim of this review was to provide insights into the pre-slaughter conditions in the horsemeat production chain and related effects on welfare and meat quality, which will in turn expand knowledge in this area and determine directions for future research. Throughout the pre-slaughter period, horses encounter multiple potential stressors that can compromise their welfare and negatively affect carcass and meat quality. These stressors can arise at various stages, including conditions at the point of purchase, loading, transport, unloading, lairage, stunning and exsanguination. Pre-slaughter welfare conditions are significantly affected by animal characteristics (age, gender, breed, temperament), the infrastructure at both the point of purchase and the slaughterhouse, vehicle design, environmental conditions and the quality of human-animal interactions. Since current recommendations on minimum floor space in transport vehicles are insufficient and also clear guidelines on optimal lairage duration for horses at slaughterhouses are lacking, further research is required to enhance horse welfare across the meat production chain

    Najčešći razlozi gubitaka jaganjaca u ranom uzgoju na našim farmama i kako ih spečiti

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    Novorođena jagnjad su veoma osetljiva kategorija životinja, posebno kod visokoplodnih rasa. Kod farmera se ovi gubici često zanemaruju i gotovo podrazumevaju. Opstanak ove grane stočarstva je uslovljen, ili krajnje ekstenzivnim uzgojem (velik broj ovaca koje rađaju po jedno jagnje i uspešno samostalno brinu o njemu) ili maksimalno intenzivnim uzgojem u zatvorenom sistemu držanja, sa rasama koje donose od 3 do 6 jaganjaca godišnje, gde se gubici moraju što bolje kontrolisati. U ovom radu su izneta neka naša iskustva iz ove oblasti, kao i smernice koje mi, kao veterinari, uglavnom ne razmatramo zbog toga što se bolesti uglavnom pojavljuju u akutnoj formi. One prolaze bez ili sa neuspešnom kurativom, umesto da se baziraju na preventivnim, istovremeno i znatno uspešnijim zahvatima, kao i postupcima koji su našli „nemedicinsku“ ali vrlo praktičnu primenu. Smatramo da veterinari moraju biti nosioci ovog znanja koji doprinose uspešnom vođenju farmi

    Molecular and serological herd-level prevalence of Coxiella burnetii in bovine dairy herds in Montenegro

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    Introduction: Shedding of Coxiella burnetii through milk is significant, particularly in dairy cattle, making milk a potential source of infection for humans. The aims of this study were to estimate the individual and herd-level prevalence of C. burnetii on dairy cattle, and to assess potential public health risk. Methodology: The study was conducted as a screening study in 95 randomly selected dairy herds from Montenegro from March to May 2019. No abortions, reproductive disorders, or human diseases were reported in these farms. In order to identify positive farms, anti-C. burnetii antibodies and C. burnetii DNA were detected in bulk tank milk (BTM) using enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qPCR), respectively. All animals from the positive farms were sampled twice, 2 months apart; the presence of C. burnetii DNA in individual milk samples and the presence of anti-C. burnetii antibodies in milk and blood serum was detected using qPCR and ELISA. Results: The overall herd-level prevalence of C. burnetii was 9.47% (9/95). Analysis of individual milk samples in the positive farms revealed anti-C. burnetii antibodies and C. burnetii DNA in 13.48% and 4.49% of the cows, respectively. Antibodies were also detected in 15.73% of the blood samples. No significant differences were observed between the results obtained through serological and molecular examination on the same farm two months later. Conclusions: Although a low presence was detected in the farms, public health risk cannot be excluded. Further research is needed for unravelling the current epidemiological situation in the country

    Welfare on dairy cows in different housing systems: emphasis on digestive parasitological infections

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    The aim of this research was to assess welfare indicators in different dairy cow management systems, determine the prevalence of parasitic infections, and examine the impact of these infections on welfare indicators. This study was conducted in 2024 on 45 Holstein-Friesian cows aged 2 to 6 years (first to third lactation) in Northern Serbia. Monitoring was carried out in tie stall, loose, and pasture-based systems, covering three production phases: late dry period, clinical puerperium, and peak lactation. Cow welfare was evaluated using the Welfare Quality® protocol, and parasitological diagnostics from fecal samples. Identified welfare issues included a low body condition score (BCS), dirtiness of udders, flanks, and legs, integument alterations, nasal and ocular discharge, lameness, and diarrhea. Cows in the pasture-based system had significantly higher scores for dirtiness (p < 0.001), while those in tie stalls showed more integument alterations (p < 0.001). Loose-housed cows had higher nasal discharge scores (p < 0.001). Parasites identified included Eimeria spp., Buxtonella sulcata, gastrointestinal strongylids, Moniezia spp., Dicrocoelium dendriticum, Fasciola hepatica, and Paramphistomum spp. Significant correlations (p < 0.001) were found between certain welfare indicators and parasite infections, such as a low BCS with Eimeria oocysts and nasal discharge and hairless patches with Buxtonella sulcata and Dicrocoelium dendriticum. These data indicate needs for improving dairy cows’ welfare and the implementation of effective parasite control measures in all housing systems

    The first proven case of aflatoxin poisoning of dogs in Serbia

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    Aflatoxins, secondary metabolites produced by fungi of the Aspergillus genus, are welldocumented as contaminants in the food chain, posing significant risks to various species. However, data on aflatoxin poisoning in pets, specifically dogs, remains limited. The aim of this case report is to raise awareness about the susceptibility of dogs to aflatoxins and highlight the need for stricter control and responsibility from all stakeholders, from feed manufacturers to dog owners. This is particularly critical given the absence of specific regulations regarding aflatoxins in pet food. The report describes an outbreak of aflatoxicosis in three dog shelters in Serbia, involving 19 affected mongrel dogs that died within few days of the onset of symptoms, making it one of the most severe cases on record. The affected dogs initially showed sudden jaundice, characterized by a yellow discoloration of the oral mucosa, without any preceding behavioral changes or obvious signs of health issues. Over the following days, symptoms progressed to include loss of appetite, polydipsia, apathy, and depression. Abdominal swelling developed, followed by bloody diarrhea and melena, ultimately leading to death. Blood biochemical analyses revealed a significant increase in liver enzyme activity. Despite symptomatic treatment attempts by veterinarians, all affected dogs died. Postmortem examination revealed massive hemorrhage, icterus, and ascites as the most conspicuous findings. Microscopic examination of liver tissue disclosed fibrosis and necrotic areas. In light of the clinical and pathological evidence, samples of dog food from the shelters were tested for aflatoxin content. Commercial dry dog food, which had been used for more than two months, was found to contain an average aflatoxin level of 150 μg/kg. This is the first confirmed case of chronic aflatoxicosis in dogs in Serbia, which culminated in fatal liver failure

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