Borneo Journal of Pharmacy
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Determination of Antiradical Activity, Total Phenolic, and Total Flavonoid Contents of Extracts and Fractions of Langsat (Lansium domesticum Coor.) Seeds
No full textLansium domesticum Coor. is a fruit species from the Meliaceae family, which is a tropical plant native to Southeast Asia. Local citizens call it langsat, longkong, or duku and have used it as traditional medicine. The seeds of L. domesticum are used as a fever medicine, its bark is used to treat scorpion sting, and its leaves are used to repel mosquitoes. Because of its various uses, it is necessary to explore the antiradical potential of L. domesticum seeds. This study aims to determine the antiradical potential of L. domesticum seeds extract and fractions by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and to discover compounds that act as antiradical. Lansium domesticum seed powder was macerated with methanol, and then the extract was concentrated using a rotary evaporator and fractionated by n-hexane and ethyl acetate. The antiradical assay was conducted on extract and fractions by using DPPH radicals. Phenolic and flavonoid contents from extract and fractions were also tested. The ethyl acetate fraction obtained strong antiradical potential with an IC50 value of 8.938 ± 0.031 µg/mL. Total phenolic and flavonoid contents of ethyl acetate fraction were higher with values of 58.25 ± 0.501 mgGAE/g sample and 75.123 ± 0.175 mgQE/g sample, respectively. Correlation of phenolic and flavonoid contents, which inhibited radicals had R2 values of 0.9182 and 0.7658. Ethyl acetate fraction of L. domesticum seeds had very strong antiradical activity. Further isolation is expected to be conducted to discover which compounds are the most responsible as antiradical
Antibiotics Resistance Pattern of Coliform Bacteria Isolated from Slaughterhouse Wastewater in Jega Town, Kebbi State, Nigeria
No full textWastewater is an essential reservoir of pathogenic bacteria, which include resistant strains. This study determined the antibiotics resistance pattern of Coliform bacteria isolated from slaughterhouse wastewater in Jega local government. Five different samples of wastewater from different locations of a slaughterhouse were collected using standard sample collection techniques. Coliform bacteria were isolated using the standard microbiological method. The total bacterial count was determined using plate count agar. Suspected coliforms were identified based on morphological and biochemical tests. Antimicrobial susceptibility testing was carried out using the standard disc diffusion method. The highest bacterial count (8.4x102 CFU/ml) was observed from the wastewater collection point, and the lowest bacterial (1.2x102 CFU/ml) count from the slaughtering point. Three Gram-negative bacteria, Escherichia coli, Pseudomonas aeruginosa, and Enterobacter aerogenes, were identified. Escherichia coli was the most frequently isolated in slaughterhouse wastewater 39 (45.8%). Among the antibiotics tested against isolated bacteria, Septrin was the most resistant antibiotics recorded against E. coli and E. aerogenes with 84.61% and 88.89% resistant, respectively. Pseudomonas aeruginosa was found to show higher resistance to Chloramphenicol and Septrin with 84.21% resistance each. The occurrence of antibiotic-resistant bacteria from slaughterhouse wastewater showed the risks associated with antimicrobial drug resistance transferred from food-producing animals to humans. Management concerns, such as local government health officers and community development officers, should increase the sensitization of slaughterhouse workers by organizing conferences or conducting radio talk to educates these slaughterhouse workers on the treatment of slaughterhouse wastewater and health risk associated with antimicrobial-drug resistance transferred from animals to humans
Pharmacognostic Study and Antioxidant Activity of Mundar (Garcinia forbesii King.) leaves from Banua Botanical Gardens of South Kalimantan
No full textMundar (Garcinia forbesii King.) is a plant from South Kalimantan. This plant has chemical contents that have potential as medicine. The purpose of this study is to provide a pharmacognostic picture of a specific, nonspecific and determine the antioxidant activity of G. forbesii leaves. Specific parameters include organoleptic, microscopic testing, thin-layer chromatography profiles, and phytochemical screening. Nonspecific parameters include total ash content, acid insoluble ash content, drying loss, water-soluble extract content, and ethanol-soluble extract content. Antioxidant activity was determined by the DPPH method based on IC50 values. Specific parameter test results are green powder, characteristic odor, and sour taste. Microscopic tests showed stomata, upper epidermis, lower epidermis, cell walls, xylem, phloem, palisade tissue, spongy tissue, and cuticles. Garcinia forbesii leaves contain alkaloids, flavonoids, phenols, tannins, and steroids. The TLC profile showed good separation of polar eluents, and yellow spots appeared after spraying DPPH reagents. The non-specific parameter test is the total ash content of 6.52±0.1%; acid insoluble ash content 1.06±0.08%; drying shrinkage 6.43±0.38%; water-soluble extracts 34.3±0.3%; and ethanol-soluble extracts 23.47±0.35%. Ethanol extract of G. forbesii leaves has antioxidant activity with IC50 of 65.7 ppm. Pharmacognostic study fulfills the requirements, and G. forbesii leaves extract has strong antioxidant activity
Preliminary Phytochemical Analysis and Antioxidants Activities of Ethanolic Extract from Gomphrena serrata Whole Plant
No full textThe present study was designed to investigate the phytochemical analysis and antioxidant activities of the whole plant of Gomphrena serrata. Gomphrena serrata widely distributed in South America, North America, and India. These plant parts are used as traditional medicine for the treatment of several ailments. This study aims to assess the phytochemical and free radical scavenging of ethanolic extract of G. serrata present in the plant. The preliminary phytochemical study was performed by standard method. The whole plant of G. serrata proved the presence of bioactive constituents such as carbohydrates, alkaloids, steroids, glycosides, triterpenoids, protein and amino acids, saponins, as well as flavonoids. The in-vitro antioxidant study was performed on the ethanolic extract of shade-dried of the whole plant, which determined by hydrogen peroxide, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) 100 µg/ml assay and was compared to ascorbic acid. The ethanolic extract of the whole plant of G. serrata shows the strong free radical scavenging activity. The present study was the proof for ethanol extract of G. serrata which have medicinally significant and bioactive compounds since these plant species are used as traditional medicine for the treatment of various diseases
Assessment of Drug Therapy Problems Among Type 2 Diabetes Patients with Hypertension Comorbidity in Indonesia
No full textType 2 diabetes mellitus (T2DM) is a major chronic disease that affects a large number of people worldwide. Hypertension is a common disease comorbidity among T2DM patients, and often those patients received polypharmacy and complex treatment in long term duration. This condition may lead to an increased risk of drug therapy problems (DTPs). This study aimed to assess and determine potential drug therapy problems in type 2 diabetic patients with hypertension comorbidity. Retrospective cross-sectional design was conducted in a hospital setting, especially data sources from the prescription of ambulatory T2DM patients with hypertension. A total of 190 patients were studied. More than half of the participants were female (53.68%). The majority age range of participants was 50-59 years (46.84%). Almost all antidiabetic agents were prescribed as polypharmacy (73.16%). Metformin was the most antidiabetic agent prescribed as monotherapy and combination therapy (63.16%). Almost all antihypertensive agents were prescribed as polypharmacy (63.26%). Amlodipine was the most antihypertensive agent prescribed as monotherapy and combination therapy (34.74%). Among the study participants, 56.84% have at least one of DTPs. Adverse drug reaction was the most frequent (47.22%), followed by ineffective drug therapy (29.63%). Since the potential of DTPs in T2DM patients with hypertension comorbidity is relatively high, early identifying, resolving, and preventing drug therapy problems by the pharmacist is needed to achieve goals of treatment
Novel Coronavirus: A Newly Arranged Mini-Review
No full textSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which was originally known as a virus that infects animals and rarely can be transmitted to humans in large populations, is now transformed into one of the most feared pandemics causes worldwide. Infection by a virus that is officially known as COVID-19 has caused global concern mainly due to the number of deaths caused. Since it was first reported in Wuhan, China in December 2019, now COVID-19 has spread to almost all parts of the world with a very rapid spread rate. The main symptom of this infection is a respiratory disease accompanied by other symptoms that resemble flu-like illness. Everyone can suffer from COVID-19, but the elderly, toddlers, pregnant women, and people with certain diseases and disorders of the immune system show more severe symptoms. The disease is transmitted through inhalation or contact with droplets, where the incubation period ranges from 2 to 14 days. Until now, specific therapy to treat COVID-19 has not been found, with preventive measures taken to control its spread. In this mini-review, we will explain important information related to COVID-19
Prediction of the Antiinflammatory Activity of New S-alkyl Derivatives of 1,2,4-triazol-3-thiones Using the PASS Computer Program and Molecular Docking
No full textThe strategy of rational approaches to the search for selective COX-2 inhibitors as potential antiinflammatory agents has been proposed and elaborated. It is based on the use of PASS-prediction and molecular docking. The choice of the basic structure of 4-amino-3-thio-1,2,4-triazole as a promising object of chemical modification has been substantiated. Using a modification of the primary molecule, a virtual library of S-derivatives of 5-substituted 4-amino(pyrrol)3-thio-4H-1,2,4-triazoles in the amount of 100 compounds (ten groups) has been obtained by introducing various pharmacophore fragments. Based on the analysis of the results of the PASS-prediction and molecular docking, six of the ten planned groups of compounds have been selected for the synthesis as promising selective COX-2 inhibitors. The reliability of the prediction results has already been confirmed for one of the promising group 4-amino-5-(pyridine-4-yl)-1,2,4-triazole (4Н)-3-yl-thioacetamides
Antioxidant Properties of Pyrus communis and Pyrus pyrifolia Peel Extracts
No full textWith the rise of global health awareness by embracing a healthy lifestyle and natural product consumption, the search for natural antioxidant sources has invited more research performed especially in fruits as a whole product or in its components, especially the ones that are usually discarded/not the mainly consumed component. Pyrus communis and Pyrus pyrifolia are two of the most popular species of pear fruits consumed in the world, and while there exist several studies about the antioxidant potential of its flesh, specific studies about the antioxidant properties of their peels are still lacking. To achieve an understanding of antioxidant properties of peel extract of P. communis and P. pyrifolia, several tests have been performed in controlled laboratory conditions to gauge phenolic and flavonoid contents through three different extraction measurements. This research has shown that peel extracts of both P. communis and P. pyrifolia contain natural antioxidants that are beneficial for health, with P. communis extracted with methanol having a higher concentration of antioxidant contents compared to P. pyrifolia
Determination of Gossypol in Hamid and Bt (Seeni 1) Cottonseed Oil using Fourier Transform Infrared Spectroscopy
No full textThis study was conducted to determine the gossypol content in Bt cottonseed (Seeni-1) oil by using Fourier Transform Infrared (FTIR) spectroscopy with an Attenuated Total Reflectance (ATR) element. The wavelengths used were selected by spiking refined, bleached deodorized palm oil (RBDPO) to gossypol concentrations of 0-5% and noting the regions of maximal absorbance. Absorbance values of the wavelength regions 3700-2400 & 1900-750 cm−1 and a partial least squares (PLS) method were used to derive calibration models for Hamid cottonseed oil, Seeni-1 cottonseed oil, and gossypol-spiked RBDPO. The coefficients of determination (R2) for the calibration models were computed for the FTIR spectroscopy results against those found by using the wet chemical method AOCS method Ba 8–78. The R2 was 0.8916, 0.9581, and 0.9374 for Hamid cottonseed oil, Seeni-1 cottonseed oil, and gossypol-spiked RBDPO, respectively. The standard error (SE) of the calibration was 0.053, 0.078, and 0.062, respectively. The calibration models were validated using the cross-validation technique within the same set of oil samples. The results of FTIR spectroscopy as a useful technique determining gossypol content in crude cottonseed oil showed that there is a significant difference (p <0.05) in the amount of gossypol content in Hamid and Bt Seeni-1 cottonseed oils
Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method
No full textThe free radical is an unstable molecule because contains one or two unpaired electrons. The antioxidant substance is a simple way to decrease the illness caused by free radicals. Cawat hanoman (Bauhinia aculeata L.) was known to contain tannin components one of the benefits as an antioxidant. This research aims to determine the antioxidant activity of the B. aculeata stem tested by qualitatively used thin-layer chromatography (TLC) and quantitatively using the DPPH method. Bauhinia aculeata stem was extracted using a maceration extract method with 96% ethanol. Antioxidant activity test was done qualitatively by eluent of ethyl acetate : methanol : purified water (6 : 2 : 1) using TLC and quantitatively using the DPPH method. The result of antioxidant activity from 96% ethanol extract of B. aculeata stem qualitatively showed the presence of yellow spots on a purple background at TLC after syringed DPPH 0.5 mM and quantitative test that resulted in an IC50 of 21.862 μg/mL. These results indicate that 96% ethanol extract of B. aculeata has very strong antioxidant activity