Oskar Bordeaux
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J Virol Methods
No full textThe Coronavirus pandemic unveiled the unprecedented need for diagnostic tests to rapidly detect the presence of pathogens in the population. Real-time RT-PCR and other nucleic acid amplification techniques are accurate and sensitive molecular techniques that necessitate quality control strategies and stable quality control materials. To meet this need, Twist Bioscience has developed and released synthetic RNA controls. However, RNA is an inherently unstable molecule needing cold storage, costly shipping, and resource-intensive logistics. Imagene provides a solution to this problem by encapsulating dehydrated RNA inside metallic capsules filled with anhydrous argon, allowing room temperature and eco-friendly storage and shipping. This technology initially developed for DNA storage has been successfully applied to RNA and other biospecimen and extensively validated through real time and accelerated aging. Here, RNA controls produced by Twist Bioscience were encapsulated in RNAshells and distributed to several laboratories that used them for COVID-19 detection tests by amplification. One RT-LAMP procedure, four different RT-PCR devices and 6 different PCR kits were used. The amplification targets were genes E, N; RdRp, Sarbeco-E and Orf1a/b. RNA retrieval was satisfactory, and the detection was reproducible. RNA stability was checked by real-time (3 years at room temperature) and accelerated aging (16h at 90 °C, corresponding to approximately 10 years of storage at 25 °C, according to our previously published Arrhenius study for encapsulated RNA). The results were not significantly different from those for unaged capsules. This room temperature RNA stability allows the preparation and distribution of large strategic batches which can be stored for more than 10 years a long time and used for standardization processes between detection sites. Moreover, this provides the advantage of single-use and field usability across varying temperatures. Consequently, this type of encapsulated synthetic RNA, processed at room temperature, can be used as internal quality control materials for the SARS-Cov-2 virus as well as for detection of other RNA viruses
« Présomption du caractère professionnel des SMS envoyés depuis un téléphone mis à disposition par l’employeur »
No full textGenetically based trait coordination and phenotypic plasticity of growth, gas exchange, allometry, and hydraulics across the distribution range of Pinus pinaster
No full textStudying intraspecific trait variation across environments is key for understanding how resource-use strategies evolve. It is hypothesized that plants from mesic environments have evolved toward a more acquisitive strategy with high growth potential and phenotypic plasticity, while populations from xeric continental climates exhibit a conservative strategy with slower growth and better physiological performance under drier conditions.We tested this hypothesis through the phenotypical characterization of 14-yr-old Pinus pinaster Aiton trees from 20 range-wide populations growing in two climatically contrasting common gardens. We measured 20 traits related to growth, leaf morphology, gas exchange, photochemistry, and hydraulics.Consistent with our hypothesis, we found that populations from mesic oceanic areas exhibited higher growth rates and higher allocation to leaf surface area under mesic conditions, along with greater plasticity in these traits. By contrast, xeric continental populations had better physiological status, showing higher gas exchange rates and photochemical efficiency, but lower sapwood-specific hydraulic conductivity under drier conditions.Together, our results provide evidence that climate drives the joint evolution of leaf and stem traits and their plasticity following an acquisitive-conservative axis of resource use. Overall, trait coordination is found to be highly plastic, likely to maximize plant performance under contrasting environmental conditions
Construire une politique de transition : le cas de la méthanisation agricole. Référentiel, luttes et controverses
No full textTrump, la guerre commerciale et le dollar : et si c’était une chance pour l’Union européenne ?
Get PDFInterdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underlies Arabidopsis response to viral infection
No full textPlant viruses represent a risk to agricultural production and as only a few treatments exist, it is urgent to identify resistance mechanisms and factors. In plant immunity, plasma membrane (PM)-localized proteins play an essential role in sensing the extracellular threat presented by bacteria, fungi or herbivores. Viruses are intracellular pathogens and as such the role of the plant PM in detection and resistance against viruses is often overlooked. We investigated the role of the partially PM-bound Calcium-dependent protein kinase 3 (CPK3) in viral infection and we discovered that it displayed a specific ability to hamper viral propagation over CPK isoforms that are involved in immune response to extracellular pathogens. More and more evidence support that the lateral organization of PM proteins and lipids underlies signal transduction in plants. We showed here that CPK3 diffusion in the PM is reduced upon activation as well as upon viral infection and that such immobilization depended on its substrate, Remorin (REM1.2), a scaffold protein. Furthermore, we discovered that the viral infection induced a CPK3-dependent increase of REM1.2 PM diffusion. Such interdependence was also observable regarding viral propagation. This study unveils a complex relationship between a kinase and its substrate that contrasts with the commonly described co-stabilisation upon activation while it proposes a PM-based mechanism involved in decreased sensitivity to viral infection in plants.Régulation de la communication intercellulaire - le rôle de la phosphoprotéine REMORIN liée aux nanodomaines de la membrane plasmiqueSaclay Plant Science
Interpersonal coordination through Granger causality applied to AR processes modeling the time evolution of low-frequency powers of RR intervals
No full textJ Clin Sleep Med
No full textSTUDY OBJECTIVES: Both the International Classification of Sleep Disorders (ICSD) and the sleep-wake disorders section of the Diagnostic and Statistical Manual of Mental Disorders (DSM) emphasize the importance of clinical judgment in distinguishing the normal from the pathological in sleep medicine. The fourth edition of the DSM (DSM-IV, 1994) introduced the clinical significance criterion (CSC) to standardize this judgment and enhance diagnostic reliability. METHODS: This review conducts a theoretical and historical content analysis of CSC presence, frequency, and formulation in the diagnostic criteria of sleep disorders. Three steps were undertaken: i) selecting main sleep disorder classifications and main disorders within them, ii) systematically extracting CSC-related content, and iii) analyzing the evolution of CSC presence through quantification (Jaccard index) and visualization (radar plots). RESULTS: The fifth edition, text revision of the DSM (DSM-5-TR) includes CSC in five of nine main sleep disorders, compared to two in the third edition, text revision of the ICSD (ICSD-3-TR) (circadian rhythm sleep-wake disorder and nightmare disorder). The overlap between DSM-5-TR and ICSD-3-TR is moderate (Jaccard index = 0.40). The overlap between DSM versions is higher (0.53). Conversely, ICSD revisions exhibit minimal similarity (0.16). Radar plots reveal a gradual increase in CSC use within ICSD versions. CONCLUSIONS: These results highlight the variable application of CSC in sleep disorder classifications. Universal CSC inclusion may not be essential, but systematic discussion of its potential use can help refine diagnostic criteria. This refinement is important for accurately diagnosing sleep disorders and better differentiating the normal from the pathological, a major challenge in sleep medicine
Neuroscience
No full textThe letter to the editor (LTE) is a correspondence forum that allows a journal's readers to comment on published research and also publish data and arguments in a brief way. The LTE has vital functions as an accessible comment forum, including holding authors and editors accountable for published content. Yet, there is also the possibility of misuse of the LTE format, as was evidenced by a recent mass retraction of 129 LTEs at a neurosurgery journal suspected to have been mass produced by the undeclared use of generative artificial intelligence (GAI). We argue in favor of a more interventionist stance on the part of neuroethicists to engage with, analyze, and propose solutions to the issue of low quality and GAI-generated neuroscience