International Journal of Advances in Pharmaceutical Analysis
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A validated RP-HPLC method for simultaneous determination of Hydrochlorothiazide and Losartan Potassium in pharmaceutical formulation
Get PDFA new, simple and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for the separation and quantification of Hydrochlorothiazide (HCTZ) and Losartan Potassium (LOS) in tablet dosage form. The determination was carried out using GRACE C18 [4.6 x 250 mm] column as a stationary phase and mobile phase comprised of Acetonitrile: Phosphate Buffer(50 : 50) pH 3.1 in proportion of 50:50(v/v); the pH of phosphate buffer adjusted to (3.1) using orthophosphoric acid. The flow rate was maintained at 1.0ml/min and the eluent was monitored at 226nm.The retention time of MET and VILD were 4.250 min and 8.300 min respectively. The method was validated in terms of linearity, precision, accuracy, specificity and robustness. The method was linear and for precision studies; RSD for HCTZ and LOS were 0.02 and 0.04 respectively. The percentage recoveries for both drugs from their tablets were 100.80 and 99.76 respectivel
Quantitative analysis of Amoxicillin and Dicloxacillin in Combined Dosage Form by First Derivative and Simultaneous Equation Method in Application to the determination of Content Uniformity
Get PDFTwo simple, accurate and precise spectrophotometric methods have been developed for the simultaneous estimation of Amoxicillin and Dicloxacillin. Method A was quantitative determination of Amoxicillin and Dicloxacillin by First Order Derivative zero crossing method. The first order derivative absorption at 231.02 nm (zero cross point of Dicloxacillin) was used for Amoxicillin and 246.26nm (zero cross point of Amoxicillin) for Dicloxacillin. Both the drugs obeyed the limit 15-35g/ml (correlation coefficient r 2 1). No interference was found between the both determined constituents and those of matrix. Method B was developed for estimation of content uniformity of Amoxicillin and Dicloxaciliin in its combined tablet dosage form. The method involves solving the simultaneous equation using 245nm and 227nm as two wavelengths for Amoxicillin and Dicloxacillin respectively. From the results, it was concluded that all brands are within the limits of content uniformity (85-115%). 0.1 N sodium hydroxide was used as a solvent for both methods. Developed method was employed to determine the Amoxicillin and Dicloxacillin content in ten individual capsule units of four marketed formulations. Both the methods were validated statistically and recovery studies were carried out to confirm the accuracy of the methods
Assessment of phytochemical constituents, trace metals and antimicrobial efficacy of holy plant Couroupita guianensis, Southern India
Get PDFPhotochemical constituents, trace metals concentration and antimicrobial activity of (three different extract such as petroleum ether, chloroform and ethanol) C. guianensis leaves were investigated. The phytochemical screening of the crude extract revealed the presence of steroids, triterpenes, alkaloids, phenols, flavonoids, saponins and tannins. The leave powder was subjected to analyse the trace metals using 797 VA Computrace voltametry, Metrohm. The average mean concentrations of Cd, Cr, Cu, Fe, Ni, Pb and Zn were BDL, 0.02, 0.32, 0.89, BDL, BDL and 0.54 mg kg -1 , respectively. The three different solvents extract of C.guianensis were analyzed for in-vitro antimicrobial activity against certain pathogens and the zone of inhibition were compared with positive control such as Methicillin 10 mcg and Itraconazole 10 mcg.The Enterococcus fecalis and Trichophyton rubrum were most sensitive (19 mm) while smallest inhibition were recorded in Micrococcus luteus and Cryptococcus sp. (7 mm)
UV-Spectrophotometric Determination of Telmisartan and Hydrochlorothiazide in Combined Tablet Dosage Form Using Simultaneous Equation Method
Get PDFA UV spectrophotometric method was developed for the estimation of Telmisartan and Hydrochlorothiazide in Combined Tablet Dosage Form Using Simultaneous Equation Method. The drug obeyed the Beers law and shows good correlation near to r 2 = 0.999 for Telmisartan and for Hydrochlorothiazide r 2 =0.999.Absorption maxima of Telmisartan 296.8 nm and Hydrochlorothiazide 271.2 nm. Beers law was obeyed in concentration rang of 5-30 g/ml for Telmisartan and 2-12 g/ml for Hydrochlorothiazide. The method has been validated for linearity, accuracy and precision. The recovery was 99.28 % for Telmisartan and 99.26% for Hydrochlorothiazide. The developed method was found to be accurate, simple, precise, economical, and selective for simultaneous estimation of Telmisartan and Hydrochlorothiazide in tablet formulations
Quantification of cinnarizine and dimenhydrinate in tablet dosage form by simultaneous equation spectrophotometric method
Get PDFSimple, accurate, precise, reproducible, requiring no prior separation and economical procedures for simultaneous estimation of Cinnarizine(CNZ) and Dimenhydrinate(DMH) in tablet dosage form have been developed. Method employs formation and solving of simultaneous equation using 250 nm and 277 nm as two analytical wavelengths for both drugs in methanol. CNZ and DMH at their respective ? max 250 nm and 277 nm shows linearity in a concentration range of 2-12 ?g /ml and 10-35 ?g /ml. Recovery studies for CNZ 98.9-100.75% and 96.16-100.69% for DMH in case of simultaneous equation method confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific
Comparative evaluation of microbiological quality of Hepatoprotective Herbal formulations marketed in Yavatmal District of India
Get PDFIn the present study herbal products marketed in Yavatmal India were determined for the presence of microbial. Microbial contents in herbal products were examined as suggested in as per W.H.O. The total of ten herbal products of various brands were selected randomly and tested for microbial contamination. Of which 3 samples did not conform to the W.H.O guidelines. The formulations are used daily by the patients suffering from Liver diseases. The specific medias were used to determining the presence of Escherichia coli (4 samples), Staphylococcus aureus (3 samples), and P. aeruginosa (4 samples). The data indicated suggest that there is requirement of in process improvement to provide better quality for consumer health in order to be competitive in international markets
Proximate analysis and mineral characterization of Barringtonia species
Get PDFThe study about material contained in Barringtonia seeds were conducted at the first time using the standard procedure of AOAC. For present work, two species of Barringtonia were selected viz., B. acutangula and B. racemosa . The proximate composition and mineral constituents of Barringtonia seeds were evaluated. The B. acutangula seeds contained ash 4.24%, crude protein 9.23%, crude lipids 0.68%, crude fibre13.54% and carbohydrates 66.17%. While in seeds of B. racemosa, ash 4.02%, crude protein 12.09%, crude lipids 0.75%, crude fibre15.18% and carbohydrates: 64.67% were observed. Both the species have a high energy value on dry basis. Comparing the mineral content with recommended dietary allowances (RDA), the results indicated that seeds of B. acutangula fruits could be a good supplement for some nutrients such as calcium, fibre and carbohydrates, whereas the B. racemosa seeds could be a good supplement for some nutrients such as fibre, potassium, zinc and carbohydrates. The seeds of Barringtonia could be promoted as a carbohydrate supplement. Hence, this piece of work acclaims the potential of Barringtonia species indicating its proximate and mineral composition
Stress degradation of Lisinopril as per ICH Guidelines & Characterisation
Get PDFLisinopril an antihypertensive drug was subjected to stress degradation, since the drug is photosensitive undergo hydrolysis and oxidized in presence of oxygen. Hence the objective of the study was to stress degrade Lisinopril and to find out the pathway for stress degradation of Lisinopril. Stress testing methods are screening methods to be used to understand the degradation chemistry of a drug. Lisinopril was subjected to stress degradation under different conditions recommended by International Conference on Harmonization (ICH). The chromatographic separation of Lisinopril and its degradation products was done on C18 column and mobile phase was mixture of Methanol and Water in ratio 80:20, pH 3.5 adjusted with orthophosphoric acid at a flow rate of 1ml/min using UV detector with ?max 220nm. The quantification and characterizations of degraded products were carried out by UV, IR spectroscopy and HPLC. The mechanism of degradation was confirmed by GC-MS fragmentation pattern
Identification bioactive compound of Nycanthes arbortrisis linn by GC-MS
Get PDFObjective : To determine possible bioactive compounds of the different extract fraction of entire plant of Nycanthes arbortrisis linn. Methods : The present research work was carried out by using GC-MS analysis, while mass spectra of the compounds found in the extract fraction was conducted by using the database of national institute of standard and technology (NIST) having more than 62000 patterns. Results : Forty components from different extract fraction of entire plant of Nycanthes arbortrisis linn were identified. Active principles with their retention time, area, height of peak, percentage of area of peak were obtained. Conclusions: This is the first report of documentation of active constituents from entire plant of Nycanthes arbortrisis linn. The research reveals the potential of entire plant of Nycanthes arbortrisis Linn as a good source of bioactive such as fatty acid esters, alcohol, hydrocarbon, aldehyde, ketones, amide, terpenes, sterols that justify used of this plant for its various ailments for traditional Practitioner
Review: common trouble shooting problems in RP-HPLC
Get PDFHigh performance liquid chromatography is one the powerful analytical tool regularly employed for the analysis of the drugs in the pharmaceutical formulations. Day by day advancement in instrumentation is increasing but still the problems have been encountered while performing analysis. Here in this review article different troubleshooting has been described with their causative and preventive parameters during performing the method development for separation and identification by RP-HPLC