International Journal of Advances in Pharmaceutical Analysis
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Assessment of physiochemical and microbial concentrations in different fresh waters in Tiruchirappalli city: A special emphasize on their correlation
Get PDFThe six fresh water samples belonging to three different sources (Cauvery river water, ground water and bottle drinking water) from Tiruchirappalli city were collected (bi-monthly sampling) during April 2014 to June 2014 for physiochemical and microbiological study. Bacterial and physiochemical parameters levels were two to ten folds higher in river water sample than in ground and bottle water due to discharges of municipal sewage and human activities. The elevated bacterial communities in May month indicated that which received waste materials from landside and more visits due to the lack of water scarcity. The higher pollution index (PI) ratio (>1) were observed in river water samples (than the ground and bottle water) in all the three months indicate the human fecal matters were responsible for water pollution. The order of decreasing microbial and physiochemical values were: May month > April month > June month. The correlation proved that physicochemical parameters were strongly supported to microbial communities due to addition of rich organic content in the water bodies from different pollution sources. Based on the report, this study was suggesting that throughout impoundment is needed to protect fresh water sources
Development of validated UV spectrophotometric method for the simultaneous estimation of metformin hydrochloride and glipizide in tablet dosage form
No full textA simple precise reproducible U.V. Spectrophotometric method have been developed and validated for the simultaneous estimation of MET and GPZ in tablet dosage form. This paper describes the simultaneous equation method as a quantification parameter which involves the measurement of absorbance of MET and GPZ at 237 nm and 234.0 nm respectively. MET and GPZ both obeyed linearity in the range of 2?g/mL to 20 ?g/mL. The recovery studies shows %RSD for MET 0.005, 0.12, 0.65 and for GPZ 0.008, 0.54, 0.25 by SEM method. The results of analysis have been validated statistically for accuracy, Precision, Repeatability, and Ruggedness. The method was successfully applied to the determination of these drugs in pharmaceutical dosage for
Validated RP-HPLC method for determination of related substances of montelukast from montelukast sodium chewable tablets
Get PDFThe development of a RP-HPLC method for Montelukast in the presence of its impurity and degradation product generated from force degradation studies drug was exposed through various degradation stress conditions and found to be stable column used BDS Hypersil C18 (250 mm x 4.6mm) 5um. Mobile phase was used in mixture of Buffer and Acetonitrile (30:70, v/v). The HPLC method was developed and validated with respect to linearity, precession, accuracy, ruggedness and specificity
Statistically Comparable UV-spectrophotometric Method development for the Estimation of Probenecid in tablet Dosage Form with the Application of Hydrotropic Solubilization Phenomenon.
Get PDFStatistically comparable method has been developed, validated, for estimating probenecid in tablet dosage form with the application of hydrotropic solubilization phenomenon. This research effort utilizes 2M sodium acetate as hydrotropic solubilizing agent which enhances the aqueous solubility of probenecid by 85 folds. The drug stability in hydrotropic solution was found to be more than 48hrs.The probenecid hydrotrope obeys the Beers Lambert law at maximum wavelength of 244.5nm. The drug response was found to be linear in the range of 2 26 g/ml with correlation coefficient of 0.9987.The non-interference of hydrotropic agent and additives in the course of probenecid estimation ensures the specificity of the proposed method.The mean recovery of 99.43% reflects its accuracy, obtained in good terms. The method precision results were found to be within limit expressed in %RSD (NMT 2%). The results of comparison of proposed method with standard method (IP 2010) were found to be in the similar range. The comparison provides evidence of proposed method to be simple, less time consuming, safe and eco-friendly. The validation (as per ICH 2005) of the proposed method accesses its use in the routinely quality control of probenecid in tablet dosage form
New validated RP-HPLC method for simultaneous estimation of chlorpromazine and trihexyphenidyl HCl in tablets
Get PDFA simple, specific and precise reverse phase high performance liquid chromatographic method was developed and validated for simultaneous estimation of Chlorpromazine and Trihexyphenidyl HCl in tablets. Quantification was achieved by using a reverse-phase C18 column (Inertsil ODS 3V, 250 mm x 4.6 mm; 5) at 31 o C. The mobile phase consisted of a mixture of ammonium acetate buffer and methanol in the ratio of 15:85 v/v at a flow rate of 1.2 mL/min. The retention times of Chlorpromazine and Trihexyphenidyl HCl were found to be 3.237 min and 5.260 min respectively. The developed method was validated as per ICH Guidelines for linearity, accuracy, precision, detection limit, quantification limit, ruggedness, robustness, specificity and system suitability. The percentage recoveries for both of the drugs from their tablets were found to be 100.34 % and 99.80 % respectively. The method may successfully be employed for the simultaneous determination of Chlorpromazine and Trihexyphenidyl HCl in pharmaceutical tablet dosage forms
Bioanalytical method development and validation
Get PDFBioanalytical methods are used for the quantitative analysis of drugs and their metabolites in the biological media like saliva, urine, plasma, serum. Development and validation of bioanalytical method is important to understand the pharmacokinetics of any drug and/or its metabolites. Bioanalytical method development consists of three essential interrelated parts sample preparation, chromatographic separation and detection by using proper analytical method. Validation of a Bioanalytical method is the process by which it is established that the performance characteristics of the method meet the requirements for the intended Bioanalytical application. The validation is further divided into 3 segments full validation, partial validation, and cross validation each of which has its own purpose. This review describes mainly the various aspects for development of bioanalytical method and for the validation of bioanalytical methods. Also the Bioanalytical method transfer is also described
Secondary metabolites screening from in-vitro cultured Rauwolfia tetraphylla by HPTLC-MS: A special emphasises on their antimicrobial applications
Get PDFThe current study designed at evaluating the phytochemical, trace metal concentration and antimicrobial properties were screened by the ethanolic extracts of in-vitro cultured medicinal plant Rauwolfia tetraphylla . The In-vitro shoots proliferation from nodal explants of R. tetraphylla using Murashige and Skoog (MS) medium containing 0.1mM of NAA and 0.25mM of BAP was effectively induce the shoot buds. The phytochemical analysis of cultured plant extracts revealed the presence of steroids, reducing sugars, sugars, alkaloids, phenols, flavonoid, saponins, tannins and amino acids. In continuously, we assessed by HPTLC coupled with mass spectrum, based on the mass spectrum were easily identified the major compounds such as 3-isoreserpine, ajmalicine, ajmaline, reserpine and yohimbine from R. tetraphylla . Metal contents of plant samples, Cd, Cr, Cu, Fe, Ni, Pb and Zn concentrations are BDL, BDL, 0.12, 0.68, BDL, BDL and 0.62 mg kg-1, respectively. The ethanol extraction of in-vitro R. tetraphylla inhibits the growth of bacteria and fungi to a greater extent
Development of stability indicating assay method for the simultaneous estimation of Metformin hydrochloride and Glipizide by RP-HPLC method
Get PDFA simple, sensitive an isocratic RP-HPLC method for the estimation of Metformin Hydrochloride (MET) and Glipizide (GPZ) in combined dosage form using Inertsil C8 column (250.6 mm, 5 ) in an isocratic mode with mobile phase comprising Acetonitrile: Water (70:30) and one drop of Triethylamine. The flow rate was 1.0 ml/min and effluent was monitored at 222 nm. The retention times were found to be 3.40 min for MET and 4.44 min for GPZ. The assay exhibited a linear dynamic range of 100-500 g/ml of MET and 1-5 g/mL for GPZ .The calibration curves were linear (r = 0.9985 for MET and r = 0.9955 for GPZ) over the entire linear range. Recovery was found to be 99.94 % + .17 for MET and 99.61 % + 0.89 for GPZ. % RSD of system precision were observed 0.0429 for MET and 0.7212 for GPZ
Development of validated stability indicating assay method for simultaneous estimation of Diclofenac Sodium and Misoprostol in their combined dosage form
Get PDFA Stability indicating Reverse-Phase liquid chromatographic method for the simultaneous estimation of DF and MP was developed. The chromatographic assay involves the use of Hi Q C18 W, 150 x 4.6mm, 5m column with a simple mobile phase composition of Acetonitrile and HPLC Grade water in the ratio of 70:30%v/v at a flow rate of 1mL/min with U.V detection at wavelength of 220 nm. The method showed good linearity in the concentration range of 50-100 ?g/mL for DF and 0.20-0.40 ?g/mL for MP. The proposed method was also successfully applied to 20 tablets of marketed formulation (Arthotec). The developed method was successfully validated as per the ICH guidelines for following parameters. Accuracy, precision, repeatability, ruggedness, robustness, system suitability tests, etc. The RSD for Intra-day and Inter-day precision was found to be 0.96-1.85, 1.02-1.83 For DF and 0.55-0.59, 0.59-0.63 for MP. The average percentage recoveries for DF were found to be 90.83, 99.74, 100.21 and for MP it was found to be 100.83, 98.94, 99.72. which was in good agreement with labeled amount of Pharmaceutical formulation. The stability indicating capacity was tested by accelerated degradation of marketed formulation in acidic (0.1 N HCl), basic (0.1 N NaOH), Neutral (water), Oxidative (3% H 2 O 2 ), Thermal (60 0 C), Sunlight exposure
A Validated RP-HPLC Method for the Determination of Telmisartan In Bulk and Pharmaceutical Dosage Form
Get PDFA RP-HPLC method has been developed and validated for the estimation of telmisartan in bulk and pharmaceutical dosage form. A RP-HPLC isocratic separation was achieved on C18 column (250.6 mm i.d., 5m) utilizing a mobile phase comprising of methanol and acetonitrile in the ratio of 90: 10(v/v) and the eluents from the column were detected using a variable wavelength detector at 237nm. The proposed method has permitted the quantification of telmisartan in the linearity range of 20-100g/ml and the flow rate was maintained at 1ml/min. The column was maintained at ambient temperature and the complete separation was achieved for telmisartan in an overall analytical run time of approximately 10 minutes. The retention time of telmisartan was found to be 3.3 minutes. The limit of detection and limit of quantification were found to be 2.82 and 8.54 ?g/ml, respectively. The percentage recovery was found to be in between 87.3 to 103.18%. The method was found to be suitable for the routine quality control analysis of telmisartan in bulk drug and formulation. The method was validated as per ICH guidelines