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Ovicidal action of insectoacaricide drugs sentry home, Neostomazan 1:200 manufacured by CEVA, Neostomazan 1:200 manufacured by product and Extrazol M on fleas Ctenocephalides spp. eggs
Get PDFThe aim of the research. To compare the ovicidal efficiency of insectoacaricides of different composition and manufacturers on flea eggs (Ctenocephalides spp.) for treatemnt of the premises where animals live.
Materials and methods of the research. The study was conducted on the basis of the clinic of veterinary medicine "Vetservice" Sumy, laboratory "Veterinary Pharmacy" and "Innovative technologies and safety and quality of livestock products" of Sumy National Agrarian University. The ovicidal effect of insectoacaricides on flea eggs of Ctenocephalides spp. was studied. Ctenocephalides spp. eggs were selected from the pet bedding on which the animal spended most of its time, namely the cats. The studied material was selected with a cosmetic brush. Ctenocephalides spp. eggs were placed into a Petri dish of 10 eggs per each dish. The test material was introduced with a dental probe. There were 4 test dishes, which were treated with insectoacaricides (each test dish was treated with a separate drug) and 1 control dish with no treatment. Microscopy was conducted under a light microscope with magnification X8 of each egg, with following treatment of each egg with insectoacaricides. Monitoring was conducted in 24, 48 and 72 hours after treatment.
Results. Research has shown that drugs which demonstrated 100 % ovicidal effectiveness were Sentry Home (pyriproxyfen – 0.02 %, permethrin – 0.2 %, n-Octyl Bicyclohepten – 1.0 %) in 24 hours and Neostomazan (CEVA)(transmix – 5.0 g, tetramethrin – 0.5 g) in 72 hours.
Conclusions. Insectoacaricide drug Sentry Home (pyriproxyfen – 0.02 %, permethrin – 0.2 %, n-Octyl Bicyclohepten – 1.0 %), used for the treatment of the premises where the animals live, showed the most pronounced ovicidal effect in 24 hours
Evaluation of doctors’ assessment of healthy lifestyle: cross-sectional study
Get PDFThe aim. To study the factors of doctors’ assessment of their lifestyle as a healthy one, as well as their readiness to take practical measures for health maintenance.
Materials and methods. 167 doctors representing all macro regions of Ukraine were interviewed: the levels of awareness of myths about NCDs, locus of control and readiness to take practical measures aimed at health maintenance were determined. The main acting forces were determined by factor analysis. Cluster analysis was performed on the basis of the obtained factors using hierarchical (Ward’s method) and non-hierarchical (k-means method) clustering methods.
Results. The following concepts were assessed: Myths about NCDs (11 factors, 3 elements, 4 clusters), locus of control (12 factors, 3 elements, 4 clusters), factors influencing health behavior (12 factors, 3 elements, 4 clusters), readiness to take practical measures aimed at health maintenance (22 factors, 4 elements, 2 clusters). Factors that positively and negatively affect a person's assessment of the lifestyle as a healthy one were identified.
Conclusions. The selected subgroups (clusters) of respondents are a simple and effective method of building a better understanding of the target audience of campaigns to promote healthy lifestyles, which after proper clarification, verification and development of a reliable measurement tool can be used to create targeted messages for each population group after a proper clarification and verificatio
Results of bicycle exercise stress test in patients with stable coronary heart disease depending on the angiographic signs of atherosclerotic lesions of coronary arteries
Get PDFThe aim. To identify features of exercise response in coronary heart disease (CHD) patients depending on coronary artery condition and to identify factors associated with a positive test in patients with no obstructive coronary artery disease (INOCA).
Materials and methods. The study included 105 patients diagnosed with stable coronary artery disease (CAD) who were hospitalized at the City Clinical Hospital No. 8 of the Kharkiv City Council. The criteria for diagnosis of ischemia with no obstructive coronary artery disease (INOCA) were met by 53 patients who formed group I. Group II included 52 patients who were consistently hospitalized in the period from June to December 2020, and had obstructive CAD for more than 50 % according to their coronary angiography (CAG).
Results. According to the results of bicycle exercise stress test, a positive test was significantly more often registered in group II – n=30 (57.7 %) patients compared to group I – n=19 (35.8 %) patients (p=0.0249). The duration of the test in patients of group I was significantly longer than 420 seconds [290–540], compared with group II – 300.0 [210.0–540.0] (р=0.0352). Also, in patients in group II, the maximum volume of the test performed was probably lower than in group I (p=0.0324). When calculating the double product, it was also found that in group I its value was significantly higher compared to group II (p=0.0292). In group I there was a significantly higher rate of chronotropic reserve (44.0 [26.0–60.0]), compared with group II (p=0.0168). Elevated total cholesterol (above 5 mmol/l) is a statistically significant and independent factor of a positive exercise test in patients with INOCA (OR, 1.98 [0.9992-3.926], p=0.05). A correlation was found between the level of exercise tolerance and smoking in INOCA-patients (r =-0.388975, p=0.010899). Patients who underwent MINOCA also showed reduced tolerance to exercise (r=-0.3104, p=0.042721)
Conclusions. The sensitivity of bicycle exercise stress test in patients with CAD depends on the presence and severity of atherosclerotic lesions of the coronary arteries (63 % in stenotic atherosclerosis, 36 % in no obstructive coronary arteries. It was found that exercise test duration, double product, chronotropic and inotropic reserve of the heart in patients with a positive exercise test with INOCA were significantly higher compared with patients with obstructive CAD. Independent factors associated with a positive exercise test in patients with no obstructive CAD are an increase in total cholesterol (multivariate regression logistic analysis)
Effect of feeding of chelated zinc form on security, productivity and slaughter parameters of broilers
Get PDFThe results of the effect of feeding the chelated form of Zinc on the safety, productivity and slaughter parameters of broiler chickens are presented.
The aim of this study was to determine the safety, productivity and slaughter parameters of broilers on the background of feeding chelated form of Zinc.
Materials and methods. Experimental studies were conducted during 2020 on broiler chickens cross Cobb-500 in the vivarium of the Veterinary Faculty of Sumy National Agrarian University (Ukraine), divided into two groups on the principle of analogues (control and experimental) of 50 broilers each. The source of zinc for chickens in the control group was their sulfates. Chelated forms of Zinc were used for broiler chickens of the experimental group. All groups of broiler chickens were administered the compound with compound feed in a dose that corresponded to the daily requirement of the bird in this trace element. The duration of the experiment is 42 days. The growth and development of broilers were evaluated on the basis of determining the relevant zootechnical indicators. Live weight of birds was determined by individual weighing on scales of the VNC type with an accuracy of ±1 g at the age of 7, 14, 21, 28, 35 and 42 days. Both scientific and economic experiments also determined the preservation of livestock - daily by the number of culled and dead birds. Feed consumption was recorded daily, for each week of cultivation and for the entire period of the experiment. At the end of the experiment, the cost of feed per 1 kg of live gained weight was calculated.
Results. According to the results of the conducted researches, it was established that feeding of the chelated form of Zinc probably increased both the average daily gain and the total live weight of broiler chickens at different age periods of rearing. The live weight of chickens at the beginning of the experiment was almost the same, but at 7 days of age it tended to increase in chickens of the experimental group, although a significant difference between groups in this indicator was not found. It was found that the weight of uneviscerated, semi-eviscerated and eviscerated chicken in poultry of the experimental group was greater than similar indicators in the young animals of the control group by 9.3, 9.2, and 9.8 %, and this difference was significant (p<0.01). The yield of semi-eviscerated carcasses was higher in ones of the experimental group (84.19 %), and the difference was significant (p<0.05).
Conclusions. It was found that the live weight of chickens of the experimental group (consumption of feed with a chelated form of Zinc) prevailed at 7 days of age by 1.1 g; on the 14th day – on 22.1 g (p<0.01); at 21 days – 35.4 g (p<0.01); at 28 days – 94.5 g (p<0.05); at 35 days – 133.6 g (p<0.01); at 42 days – at 218.9 g (p<0.05). Preservation of young animals in the experimental group, which used chelated compounds of Zinc, was higher and amounted to 96 %, compared with the control – 92 %. By age, feed costs per unit weight gain were lower in the experiment, respectively: 0.05; 0.08; 0.12; 0.06; 0.07 and 0.05 kg, compared to the control group. Growing broiler chickens on compound feed with a chelated form of Zinc by 9.8 % (p<0.01) increases the weight of the eviscerated carcass
Comparative aspects of treatment of endometrial hyperplasia in women of reproductive age with overweight
Get PDFThe aim. Minimization of the frequency of recurrence of endometrial hyperplasia (EH) in women of reproductive age with overweight (OW) depending on the tactics of treatment.
Materials and methods. 90 women of reproductive age with endometrial hyperplasia and OW were selected. They, in turn, were divided into three groups: group 1 – 30 women who took a gonadotropin-releasing hormone agonist (GnRH agonist), namely at a dose of 3.75 mg intramuscularly once every 28 days; group 2 – 30 women who used progestin (norethisterone) at a dose of 10 mg per day from day 16 to 25 of the cycle, group 3 – 30 women who took combined oral contraception (COC) (30 mcg ethinyl estradiol and 150 mcg desogestrel) in a cyclic mode 21/7.
Evaluation of the effectiveness of therapy included a clinical picture of the disease 6 months after the start of treatment, assessment of the variability of the average values of endometrial thickness and uterine size on ultrasound (US) of the pelvis 6 months after treatment. Also analyzed the effectiveness of therapy based on the results of morphological examination of the endometrium in a biopsy of the uterine mucosa, performed 6 months after the start of treatment. In addition, a general analysis of the frequency of EH recurrence was performed 6–24 months after treatment.
Results. The results showed that in the group in which women were prescribed GnRH agonist, there was a significantly higher effectiveness of treatment, in particular the absence of uterine bleeding and menstrual disorders (MD). At the same time, in the other norethisterone group, 53.3 % (16) of women had intermenstrual uterine bleeding. In patients in the group in which women received COC, uterine bleeding was observed in 30.0 % (i.e. 9) of patients (p <0.05).
Immediately after treatment, the average values of endometrial thickness in patients of group 1, according to ultrasound, was 3.59±0.47 mm, which was significantly less than in women of groups 2 and 3 – 6.81±0.59 mm (p<0.001) and 7.58±0.69 mm (p<0.001).
In addition, patients in group 1 at 3, 6, 12, 24 months after the end of hormone therapy were registered significantly lower average values of endometrial thickness, compared with patients receiving norethisterone and estrogen-progestogen drugs.
Conclusions. In a comparative evaluation of the effectiveness of treatment of endometrial hyperplastic processes in overweight women, it was found that the recurrence rate after 6–24 months occurs in 6.7 % (i.e. 2) of patients after GnRH agonist therapy, in 33.3 % (i.e. in 10) patients receiving norethisterone (p<0.001), and in 50 % (i.e. 15) of women treated with COC (p<0.001)
Catalase activity as signal of antioxydant system affection under influence of limb ischemia-reperfusion
Get PDFThe use of hemostatic tourniquet is a proved means of primary care. However, systemic disorders, as well as ultrastructural, in the area of compression can significantly worsen the condition of the injured organism.
The aim. Estimation of catalase level in rats’ liver on the background of modifications of ischemic-reperfusion syndrome to know the severest pathogenic combination for organism.
Materials and methods. 260 white adult male rats were divided into 5 groups: control (KG), EG1 – simulation of isolated ischemia-reperfusion syndrome (IRS) of the limb, EG2 – simulation of isolated volumetric blood loss, EG3 – combination of IRS of the limb with blood loss, EG4 – simulation of isolated mechanical injury of the thigh, EG5 – combination of IRS of the limb and mechanical injury. The variability of catalase level in liver was analyzed.
Results. It was found that each of the experimental interventions has led to changes of catalase activity in the liver. The most expressed pathological expressions were observed on the 3rd after interventions, when the studied index in EG3 was lower than in EG1 and EG2 in 6,2 times and by 33,1 %. On the 7th day catalase activity in EG3 was in 9,4 times and by 44,5 % times lower than in EG1 and in EG2 data concordantly. The combination of limb ischemia-reperfusion with blood loss in EG3 led to exhausting of liver antioxydant enzyme catalase in the most critical posttraumatic period (day 3). The same, but less significant effect was registered in the group of combination of mechanical trauma with ischemia-reperfusion in EG5. This proved the role of the tourniquet as a factor that complicated the course of traumatic disease due to ischemic reperfusion.
Conclusions. In this experiment, founded risk factors of combination of ischemia-reperfusion with heavy blood loss emphasized the importance and particular attention on such widespread method of bleeding tratment, as the imposition of a tourniquet, as in our experiment it triggered risk factors of ischemia-reperfusion. It was shown katalase activity depression respectively to the periods of increasing of lipid peroxydation. There was peculiarity, that on the base of isolated IRS catalase activity was increased in 2,5 times comparely to control group, whereas the hardest depression of it was found on the background of IRS, combined with blood loss – catalase activity was lower, comparely to KG – in 2,5 times. The importance of understanding the suppression of hepatocytes’ antyoxydants is great, as it might help in prevention the development of liver failure or hepatorenal syndrome on the background of limb ischemia-reperfusion
Outcomes of surgical reconstruction of the superior vena cava syndrome in patients with locally advanced thymomas
Get PDFThe aim of the study: to study and demonstrate the potential and technical aspects of the surgical treatment of locally advanced thymus tumours with the tumour infiltrate affecting superior vena cava and its branches.
Methods. 56 patients with locally advanced thymomas complicated by SVCS were study. The control group included 30 patients with SVCS were treated with conventional techniques, while the experimental group included 26 patients who underwent a novel bypass surgery developed by us.
Results. Based on our observations, patients tolerated these surgeries much better. The venous bypass was mandatorily complemented with cytoreduction. Auriculo-jugular (left and right) and auriculo-subclavian (left and right) bypasses were used in our observations
Complications in the post-operative period were reported from the experimental group and included auriculo-subclavian bypass thrombosis, post-operative complications were reported in the control group including haemorrhage from the sternotomy wound in 1 (3.3 %) case, superior vena cava thrombosis in 2 (6.6 %) cases, pneumonia in 2 (6.6 %) cases and thromboembolism of small pulmonary arteries in 2 (6.6 %) cases.
Post-operative lethality in the study groups was reported in the control and in the experimental group. Total lethality rate was 8.9 % (5 patients). The relative risk of complications and lethal outcome was calculated for patients from both groups. It was found that the risk of complications was twice as high in the control group as in the experimental group (standard error of relative risk equals 0.64), whereas the risk of lethal outcome increased by a factor of 3.5 in the control group (standard error of relative risk equals 1.09)
Conclusion. It has been established that the superior vena cava syndrome in patients with locally advanced thymoma is an emergency condition whose surgical correction must be personalised depending on the anatomic and topographic classification of SVC lesion types. It is known that an obligatory pre-condition of the perioperative period in this category of patients is an adequate vascular approach to the superior vena cava system. The first mandatory step of the radical surgery in patients with locally advanced thymomas with SVC invasion should be the auriculo-jugular and auriculo-subclavian bypasses, which can reduce the relative risk of post-operative complications by a factor of the risk of lethal by a factor of 3.5
The reasons of unsatisfactory results of extracorporeal shock wave lithotripsy in patients with ureterolithiasis
Get PDFThe article dedicate to the problem of failure of extracorporeal shockwave lithotripsy in patients with ureterolithiasis and reveal the changes which appear in the ureter in the location of the stone.The aim. Analysis of the results of treatment in patients, suffering ureteric stones, using the ureterolithotripsy procedure after failure extracorporeal shockwave lithotripsy.
Materials and methods. In 137 patients with ureteric stones, whom ureterolithotripsy procedure after failure extracorporeal shockwave lithotripsy was conducted, the symptoms of the disease, the diagnostic methods value, efficacy of surgical treatment and reasons of the failure of previous method of treatment were analyzed.
Results. In 135 patients endoscopic removal of stones has been succeeded, in 2 patients because of total obliteration of the ureter, uretero-ureteral anastomosis has been performed. If the symptoms, with are characteristic of ureterolithiasis, persists up to one week stones don’t cause significant macroscopic changes to the ureter wall. If the stone persists in the ureter longer than a week we identified local appearing of oedema. Long–term (more than two months) ureteric stone persistence increase the risk of intramucosal “ingrowth” of the calculi greatly.
Conclusion. The URS and ESWL are high effective and minimal invasive methods of surgical intervention for patients with ureterolithiasis, guaranteeing high level of postoperative “stone free rate”. Prolongation of the stone insertion time in the ureter causes the ureteric wall changes, complicating performance of minimal invasive interventions (ureterolithotripsy and extracorporeal shock–wave lithotripsy) and reduce its efficacy
Female patients with androgenetic alopecia: immunohistochemical picture of scalp biopsies
Get PDFThe aim of the research. To expand the knowledge about immunohistochemical changes in female patients with androgenetic alopecia (AGA) focusing on non-androgenic co-factors of pathogenesis of the disease, as this may serve as a basis for improving therapeutic regimens.
Materials and methods. Scalp biopsies of female patients with androgenetic alopecia were examined by immunohistochemical method. The study included two groups: the main group of 30 female patients aged 22 to 40 years, average age 32,13±5,03 years, and the control group that included 20 skin samples from women aged 25–40 years (average age 34,75±4,19 years) who underwent autopsies.
Results. It was found that AGA in women is characterized by a number of immunomorphological manifestations: the inflammatory infiltrate that consists of immunocompetent T-lymphocytes CD3+, CD4+ and CD8+, macrophages (CD68+); imbalance of growth polypeptides VEGF, TGF-β1, EGFR; accumulation of oxidative stress enzymes eNOS and iNOS; accumulation of pathological fraction of Collagen IV.
Conclusions. The data obtained by this study helps to improve the concept of morphogenesis of AGA, and also can become a base to improve the standards of treatment of the disease. The pathological triade “oxidative stress-microinflammation- fibrosis” should be considered as a possible treatment target, as well as the imbalance of growth peptides
Study of biological and ecological features of permanent ectoparasites of cattle
Get PDFThe aim of the research was to study the epizootological features of bovicolosis and sifunculatosis and to determine their biological features.
Materials and methods. Eight groups of animals were examined, 15 animals in each, including cows, heifers and calves. Groups of calves consisted of 13 heifers and 17 bulls. A total of 103 females and 17 male cattle were examined.
Results. During observations, it was noted that in most cases, certain species of ectoparasites were found on different animals. In the case of their simultaneous parasitism on one animal, populations occupied different parts of the host's body. The causative agents of sifunculatosis (L. vituli) and bovicolosis (B. bovis) were determined.
During the study of epizootological features of sifunculatosis and bovicolosis, attention was paid to the seasonality of the invasion – the minimum intensity of the invasion was observed in the warm season, the maximum – in winter. The extent of invasion among calves reached 100 %.
The biological features of ectoparasites were studied. According to the data obtained, it was determined that linognathus are greater than B. bovis at all stages of development. Egg size 0.8–0.9 mm, larvae go through all stages of molting from 0.7 mm to 3.3 mm, adults reach a size of 3.3–4.0 mm. However, the eggs of B. bovis reached 0.3–0.5 mm, the size of the larvae 0.4–1.0 mm, adults ranged from 1 mm to 1.8 mm.
Conclusions. It was noted that the intensity of the infestation of bovicolosis and sifunculatosis increases in the cold season. The young are most prone to lesions