ScienceRise: Medical Science
Not a member yet
556 research outputs found
Sort by
Роль дисбалансу факторів агресії та захисту у шлунковому соку у розвитку кандидозу слизової оболонки верхнього відділу травного тракту
Get PDFResearch is devoted to the study of endogenous factors that would explain conditions of the spread of Candida albicans infection into the distal parts of digestive tract and their possible connection with comorbid pathology of thyroid gland. The aim of research is the study of pepsin, glycoproteins, hexosamines, fucose, sialic acid level in the gastric juice of patients with candidiasis of mucous tunic of the upper section of digestive tract.Methods. There were examined 116 patients, who were divided into three groups according to the results of microbiological examination: 1 group – 57 patients with IV degree of massivity of Candida fungi seeding that is with oropharyngeal candidiasis (OPC) and surface fungi growth the in mucous tunic of digestive tract and/or stomach; 2 group – 42 patients with invasive Candida fungi growth in the mucous tunic of digestive tract and/or stomach; 3 group – 17 patients without OPC and fungi growth in biopsy material. The subgroup 1А included 12 patients of the 2 group with surface fungi growth in biopsy materials of digestive tract and/or stomach. In the stomach content was determined pepsin, glycoproteins, sialic acids, fucose, hexosamines concentration. The control group included 20 practically healthy persons. The ultrasound examination of thyroid gland was carried out. Statistical analysis was realized using Pirson’s χ2 criterion, Fisher’s distinct criterion (F), Student’s t-criterion. Correlation analysis was carried out using Pirson’s correlation coefficient (r) for parametric values and Spearmen’s one (ρ) for nonparameric values.Results. According to the results of research was detected the rise of hexosamines synthesis in patients with candidiasis of 1 and 2 group comparing with patients without candidiasis (р<0,001) and (p<0,01), respectively, and increase of glycoproteins in patients with Candida invasion in mucous tunic comparing with patients with surface fungi growth (р<0,05). The decrease of fucose was typical for patients of all groups comparing with control, but the changes of indices of the main structural components of mucin at the expense of its decrease were more expressed in patients with fungi invasion in the mucous tunic that was proved by their direct correlations with surface and invasive Candida albicans growth in the stomach body. Structural changes such as hyperplasia of thyroid gland and nodular goiter that were detected in one third of examined patients with candidiasis of mucous tunic were associated with indices of mucin structural degradation.Conclusions. In patients with candidiasis of mucous tunic of the upper part of digestive tract was revealed imbalance in the ratios of structural components that was formed at the expense of decrease of hydrophobic final fucose component. Negative influence of mucin structural degradation at the expense of fucose decrease on the course of candidiasis of mucous tunic of the upper part of digestive tract is proved by the direct correlation with indices of Candida albicans growth of the mucous tunic of stomach body according to the results of microbilogical study of biopsy materials. The direct correlation between indices of ratios glycoprotein/fucose and hexosamines/fucose and structural changes of thyroid gland such as hyperplasia and nodular goiter that were more often revealed in patients with candidiasis of mucous tunic of the upper part of digestive tract need the further studyРезультати досліджень демонструють здатність Candida albicans стимулювати синтез глікопротеїнів при інвазії у слизову оболонку на тлі структурної деградації муцину з підвищенням індексів співвідношень основних компонентів слизового гелю за рахунок зниження фукози. Асоціація індексів деградації та гіперплазії щитоподібної залози і вузлового зобу зумовлює необхідність визначення можливої єдності патогенетичних процесів їх розвитк
Дослідження взаємозв’язків між біліарною патологією та ліпідно-фосфоліпідними змінами у хворих з неалкогольною жировою хворобою печінки та ожирінням
Get PDFThe aim of the work: The study of lipid-phospholipid indices depending on different pathology of biliary tract (BT) in patients with comorbid course of non-alcoholic fatty liver disease (NAFLD) in combination with obesity.Methods of research: There were examined 112 patients with NAFLD in combination with obesity and BT pathology: 60 patients with non-alcoholic hepatic steatosis (NAHS), 52 – with non-alcoholic steatohepatitis (NASH). Control group – 20 practically healthy persons (PHP). Depending on BT pathology patients with NAHS (n=60) and NASH (n=52) were divided into three subgroups: NAHS (1a group) or NASH (2a group) with chronic non-calculous cholecystitis (CNC); NAHS (1b group) or NASH (2b group) with chronic calculous cholecystitis (CCC); NAHS (1c group) or NASH (2c group) in patients with postcholecystectomical syndrome (PCES). The qualitative composition and quantitative content of lipids and phospholipids (PL) in the bloods serum were studied. Results: The least lipids changes were detected in patients with concomitant PCES comparing with groups of CNC and CCC. The data were proved by disperse and correlative analysis of connection between PL and BT pathology: at NAHS– рF=0,004; rs=0,306 (p<0,05); at NASH – рF=0,026; rs=0,279 (p<0,05). CNC and CCC led to the more increase of triglycerides level that PCES. According to the data of correlative analysis in groups of patients with NAHS and NASH was demonstrated that CNC and CCC led to the more essential decrease of sphingomyelin (SPM) level than PCES: at NAHS– rs=0,282 (p<0,05); at NASH –rs=0,317 (p<0,05).Conclusions. BT pathology influences the indices of spectrum of lipids and PL of the blood serum, first of all, PL, TG, SPM level at the different clinical forms of NAFLD in combination with obesityМетою роботи – встановлення взаємозв’язків між наявністю супутньої патології з боку біліарного тракту та змінами ліпідно-фосфоліпідного спектру крові у хворих з коморбідним перебігом неалкогольної жирової хвороби печінки у поєднанні з ожирінням. Результати свідчать про наявність взаємозв’язків між біліарною патологією у хворих з хронічним некалькульозним холециститом, хронічним калькульозним холециститом, післяхолецистектомічним синдромом та порушеннями у спектрі ліпідів та фосфоліпідів сироватки кров
Аналіз взаємозв'язків між індикаторами дисинхронії серця та структурно-функціональними показниками міокарда
Get PDFAim. To reveal the correlations between the values of dyssynchrony indicators and echocardiographic indices of myocardium state in patients with chronic heart failure of ischemic genesis, diabetes mellitus type 2 and heart dyssynchrony.Methods. The research included 61 patients with chronic heart failure of ischemic etiology, diabetes mellitus type 2 and heart dyssynchrony. The structural-functional features of myocardium and mechanical types of dyssynchrony were studied using echocardiography in М- and B- modes, impulse and permanent-wave Dopplerechocardiography, spectral tissue Dopplerography. Electric dyssynchrony was studied using electrocardiography.Results. Progressing of intraventricular (increase of Ts and Ts-SD) and atrioventricular dyssynchrony (decrease of LVFT) favor the increase of final systolic and diastolic sizes and volumes, growth of mass and index of the left ventricle myocardium mass and synchronous thinning of the left ventricle wall. The strengthening of myocardium dyssynchrony favors the growth of the mean parameters that characterize the heart work: striking volume, striking index, minute volume and heart index. The increase of Ts and Ts-SD indices causes the decrease of discharge fraction and the degree of shortening of the front-back size into systole that is the pumping function of heart worsens.Conclusions. The heart dyssynchrony has a negative influence on the process of heart remodeling, the load on the left ventricle increases and the pumping function decreases that favor the progressing of chronic heart failure in patients with diabetes mellitus type 2Посилення дисинхроніїсерця сприяєзбільшенню кінцевих систолічних та діастолічних розмірів й об'ємів, росту маси міокарда лівого шлуночка та його індексу, потоншанню стінки лівого шлуночка, росту ударного об'єму, ударного індексу, хвилинного об'єму, серцевого індексу,а також зменшення фракції викиду та ступеня вкорочення передньозаднього розміру в систол
Діагностичне значення змін вмісту біомаркерів при атеросклеротичному ураженні брахіоцефальних артерій у хворих на хронічну ішемію мозку
Get PDFAim of the work – to establish the diagnostic value of biomarkers content at atherosclerotic involvement of brachiocephalic vessels for optimization of diagnostic arrangements in patients with chronic cerebral ischemia (CCI).Material and methods of research. 355 patients with CCI (among them 230 women and 125 men, mean age - 55,30±7,91years) were examined using clinical-neuropsychological, biochemical, immune-enzyme and ultrasound methods of examination. Atherosclerotic plaques (AP) of brachiocephalic vessels are visualized in 155 (43,7 %) patients. Results. According to the results of research, the reliably lower concentrations of cholesterol of lipoproteins of the high density, stable metabolites NO, catalases and increased contents of fibrinogen, homocysteine and ketodin nitrofenol hydrazones were established in the blood serum of patients with CCI at AP and also the essential changes in concentration of renewed glutathione in both plasma and hemolysate of erythrocytes and increase of activity of glutathione reductase in hemolysate of erythrocytes and glutathione peroxidase in the blood plasma were registered. The decrease of concentration of stable metabolites of nitrogen oxide at increase of AP number was established. Conclusions. The correlations were revealed between AP and the content of spontaneous ketodin nitrofenol hydrazones, homocysteine, cholesterol of lipoproteins of the high density, triglycerides, stable metabolites NO, and also activity of glutathione peroxidase in the blood plasma that testifies to the joint participation of studied biomarkers in atherosclerotic involvement of brachiocephalic vesselsДосліджені зміни вмісту біомаркерів в плазмі крові та гемолізаті еритроцитів у хворих на хронічну ішемію мозку з атеросклеротичним ураженням брахіоцефальних артерій. У хворих з наявністю атеросклеротичних бляшок виявлені достовірні відмінності щодо вмісту вивчених біомаркерів порівняно з пацієнтами, які не мали атеросклеротичних бляшок. Встановлено зниження концентрації стабільних метаболітів оксиду азоту за мірою збільшення кількості атеросклеротичних бляшо
Аналіз самовідношення пацієнтів косметологічного профілю із зниженою самооцінкою
Get PDFThe features of self-attitude in cosmetological patients were studied in the research.Contingent and methods of research. The study was carried out on the base of medical center “CA-CLINIC” (city Kiev) during 2014 – 2016 years. Testing included 204 women, who addressed for cosmetological help. The interrogated persons were divided in groups depending on the level of self-esteem: the main group included persons with low self-esteem (MG, n=108) and group of comparison – women with normal self-esteem (CG, n=96). Psychodiagnostic instrument is the questionnaire of self-attitude by V.V. Stolin and S.R. Pantileev.Results. It was revealed, that women with low self-esteem had lower levels of global self-attitude, self-respect, autosympathy, self-interest, expected attitude of others, self-confidence, readiness to self-perception, self-understanding, self-management and the highest one – self-accusation as opposite to the interrogated with normal self-esteem. The women with low self-esteem in their self-attitude expressed the negative setting as to the own Me, incertitude in own physical and psychological qualities, did not understood their negative features or overstated their value, were inclined to self-accusation and expressed the acute desire to change themselves. Self-attitude in women with low self-esteem was disharmonic and characterized with high level of self-rejection, decrease of self-respect and self-understanding, alienation of subjectively negative features, exaggeration of own defects.Conclusions. Cosmetological patients with low self-esteem form the separate category of clients and need the use of medical-psychological arrangements, directed on harmonization of disharmonic self-attitudeУ самоставленні жінки із заниженою самооцінкою проявляли негативні установки щодо власного Я, невпевненість у своїх фізичних та психологічних якостях, концентрувалися на недоліках, виказували гостре бажання змінити себе. Самовідношення у даній групі було дисгармонічним та характеризувалося високим рівнем неприйняття себе, зниженням самоповаги і саморозуміння, відчуженням суб’єктивно неприйнятних рис, перебільшенням негативних аспекті
Комплексна оцінка показників ліпідного метаболізму та системи зсідання крові для прогнозування та ранньої діагностики прееклампсії
Get PDFAim. To study informative and prognostic significance of lipid metabolism markers and coagulation system condition for PE early diagnostics in pregnant women during the second gestational trimester with risk evaluation of cardiovascular and metabolic pathologies in the given women category.Materials and methods. The analysis of blood serum samples in 267 pregnant women in II gestational trimester was carried out. The total cholesterol was determined by colorimetric method, enzymatic with CHOD/PAP, TG – by enzymatic colorimetric method, HDL – by homogeneous method, LDL – by direct method. VLDL concentration was calculated according to Fridvald's formula: VLDL = TG/2.2. AF was calculated according to the formula: AF = (TC-HDL-c)/HDL-c. Pregnant women in II and III gestational trimesters also were examined concerning the general assessment of coagulation system condition – the concentration in blood plasma of D-dimer, soluble fibrin (SFMC), fibrinogen, FIPF, protein C activity, AT III, blood plasma clotting time was determined by PT test.Results. Of the 267 pregnant women, in 43 (16.1 %) of them, which formed the main group for research, the development of PE was found. Those pregnant women, who had PE clinical picture of the different severity levels, from the second trimester had significantly elevated TG, VLDL, AF levels, and low level of HDL in blood serum with high concentration of D-dimer (from 80 to 713 ng/ml), as well as protein C low activity in 17.6 % of pregnant women. In the third trimester pregnant women, having PE, fibrinogen content was significantly higher (4.4±1.02); the accumulation of a significant amount of SF, accompanied by FIPF presence in 40 % of pregnant women, was found in 50 % of pregnant women; a significant reducing (˂70 %) of the protein C activity was found in 48 % of patients.Conclusion. Both significant increasing of the soluble fibrin in blood plasma, and reducing of the protein C activity below 70 % are thrombosis prognostic indexes. The control of lipid metabolism and coagulation system indexes is necessary during all stages of pregnancy for estimation of PE risk and cardiovascular,cerebral vascular and metabolic complications preventionВ статті продемонстрована інформативна значимість визначення показників ліпідного метаболізму (ЗХ, ТГ, ЛПВЩ, ЛПНЩ, ЛПДНЩ, КА) та системи зсідання крові (D-димеру, розчинного фібрину (РФМК), фібриноген, ФНФП, активність протеїну С, АТ-III та ін.) у жінок протягом другого триместру вагітності для можливості прогнозування та ранньої діагностики ризику розвитку прееклампсії і подальшої серцево-судинної та метаболічної патологі
Аналіз впливу терапії спіронолактоном та його комбінації з триметазидином на динаміку клінічної симптоматики та параметрів морфо-функціонального стану серця хворих з хронічною серцевою недостатністю зі збереженою фракцією викиду
Get PDFAim. To study clinical symptoms dynamics, functional condition, the quality of life, and cardiac structure and function in patients having chronic heart failure with preserved ejection fraction (CHFPEF) after Spironolactone (S) and its combination with Trimetazidine (S+T) therapy.Methods. 90 patients (aged from 60-74 years) suffering from hypertension with CHFPEF were examined using echocardiography, 6-minute walk test (SMWT), and the Minnesota living with heart failure questionnaire (MLHF) before, 6 and 12 months later after the standard therapy (K), standard with S use, and standard with S+T use.Results. On the clinical symptoms positive dynamics background, the distance of SMWT significantly increased on 26±9 m in К, 36±9 m in S, and 55±8 m in S+T; according to the MLHF, the number of points decreased: on 9,9±2,4, 17,1±2,6 і 12,9±2,3, respectively. A statistically significant decrease in left ventricular mass (LV) was on 10,4±4,4 g in К, 22,1±7,9 g in S, and 28,6±7,7g in S+T, but only in S and in S+T it accompanied by left atrium decrease – on 9,3±2,4 ml and 8,8±2,3 ml (p<0,05), respectively. The use of S and S+T led to the more expressed E/E reduction – on 2,3±0,8 cu and 2,8±0,5 cu (all р <0,05), respectively, comparing to K – on 1,2±0,4 cu (p>0,05). Systolic blood pressure (BP) in the pulmonary artery significantly decreased only in S and S+T. More distinct dynamics of echocardiographic parameters in case of S and S+T partly can be associated with more significant decrease in systolic and diastolic BP.Conclusion. Adding Spironolactone and its combination with Trimetazidine to the standard therapy of CHFPEF patients leads to the more significant functional condition positive dynamics, the quality of life, more expressed LV hypertrophy regression, system BP and BP in the pulmonary artery decrease, the improvement of LV diastolic functonУ 90 хворих похилого віку з хронічною серцевою недостатністю зі збереженою фракцію викиду під впливом лікування спіронолактоном та його комбінацією з триметазидином в доповненні до стандартної терапії через 6 та 12 місяців встановлено: більш виразну позитивну динаміку дистанції 6-хвилинної ходьби, якості життя за Міннесотським опитувальником, більший ступінь регресу гіпертрофії лівого шлуночка, зниження системного артеріального тиску та тиску в легеневій артерії, покращення діастолічної функці
Визначення активності факторів зсідання крові у хворих на гіпертонічну хворобу ІІ стадії в поєднанні з ХОЗЛ за допомогою високоспецифічних тестів з отрутами змій
Get PDFThe aim of research was to determine characteristics of plasma hemostasis changes using highly specific snake venoms in patients with HT in combination with COPD.Methods. Special laboratory tests were used: ancystron test containing snake of Agkistrodon (Copperhead) genus venom, lebetox-test containing Vipera lebetina (Blunt-nosed viper) venom, and echitox test containing Echis multisquamatus (Multiscale saw-scaled viper) venom.Results. As a result of research, the activation of hemocoagulation on the X-factor coagulation level in patients with II stage HT, thrombin and fibrin formation intensification in patients with II-III stage COPD, and misbalance in the blood coagulation system, which demonstrates a hypocoagulation tendency, combined with a strong activation of the coagulation final phase at HT and COPD comorbid course were foundМетою роботи було визначення особливостей змін плазмового гемостазу за допомогою високоспецифічних отрут змій у хворих на ГХ в поєднанні з ХОЗЛ. В результаті роботи було виявлено схильність до гіпокоагуляції на початку процесів коагуляції, яке поєднується з вираженою активацією кінцевої фази згортання крові у пацієнтів з коморбідним перебігом ГХ і ХОЗ
Фармакогенетичне тестування на поліморфізм гену CYP2C19 для оптимізації використання антитромбоцитарної терапії у хворих на ішемічну хворобу серця
Get PDFClopidogrel is a one of most important antiplatelet preparations for patients with myocardium infarction (MI) but it has the different influence effects. The aim of this research was to assess the contribution of CYP2C19 gene polymorphism in inhibition of ADP-induced aggregation of platelets by clopidogrel.Methods. We examined 52 patients (26,9 % women), mean age – 66,6±7,8 years with verified diagnosis MI. All patients underwent coronarography, stenting of infarction-depending coronary artery. All patients were divided in two groups: I carriers of *1/*1 allele and II – carriers of *2allele.Results. *1/*1 polymorphism was determined in 82,5 % of patients. Gender analysis of spreading of CYP2C19 gene polymorphism indicates that among 14 examined women *1/*1 genotype was revealed in 78,6 % (n=11), *1/*2-*2/*2 genotype – in 21,4 % (n=3). Among 38 examined men *1/*1 genotype was revealed in 81,58 % (n=31), *1/*2-*2/*2 genotype - in 18,42 % (n=7). We did not find the essential differences in dependence of sex from CYP2C19 gene polymorphism. In the group I we revealed the more inhibited spontaneous aggregation (1,52±0,53 % against 7,7±2,24 %, р=0,0000672) and ADP-induced platelets aggregation (14,16±4,96 % against 21,78±7,77 %, р=0,0127). Any essential differences in aggregation, induced by arachidonic acid (AC) were not found between two groups (15,36±5,96 % against 16,22±3,23 %, р=0,57).Conclusions. The spreading of CYP2C19 gene polymorphism is almost equal among men and women. According to the main mechanisms of clopidogrel effect, inhibition of ADP-aggregation of platelets proves that *1/*1-carriers of CYP2C19 gene react to the clopidogrel therapy better. CYP2C19 gene polymorphism does not influence on AC-induced platelets aggregationМетою дослідження є встановлення особливостей антитромбоцитарного ефекту клопідогрелю у хворих на ішемічну хворобу серця (ІХС) залежно від поліморфізму гену CYP2C19. Враховуючи, що клопідогрель є невід’ємною складовою антитромботичного лікування різних форм ІХС, які підлягають коронарним втручанням, завчасне встановлення потенційної чутливості до препарату є надзвичайно важливим з точки зору запобігання тромботичних ускладнен
Анализ показателей системы гемостаза при преэклампсии различной степени тяжести
Get PDFPreeclampsia it is a serious complication of pregnancy that is a main cause of maternal perinatal morbidity and lethality. In multifactorial genesis of development of this state the main role is played by homeostasis disturbance.Aim of research: to assess the parameters of hemostasis system in pregnant with preeclampsia of the different severity using the risk factors, genetic polymorphism of folate metabolism genes and blood coagulation.Material and methods of research. There were examined 155 pregnant women of reproductive age in 3 trimester of pregnancy: 123 – with preeclampsia of the different severity and 32 – with physiological pregnancy course (control). The analysis of risk factors was carried out by the elaborated questionnaire with point assessment. There were studied the genes of folate cycle (MTHFR C677T, MTRR ІІ22Met (66-a-g) and coagulation factors (F5 G1691A, F2 G20210A). There were assessed the parameters of quantity of platelets, fibrinogen level, values of prothrombin index (PTI), international normalized ratio (INR), activated partial thromboplastin time (APTT). The results were processed using the programs of processing of electric tables «Excel 7,0 for Windows XP».Results. It was established, that the expressed changes (decrease of platelets quantity, increase of fibrinogen level, increase of PTI value, decrease of INR and APTT) were noted in pregnant women that had the high point (≥5) of preeclampsia risk factors, early clinical manifestations of these complication (up to the 34 week of pregnancy), gene polymorphism (heterozygous variant of studied polymorphisms and their complication). There was noted the correspondence between the hemostasis system and severity degree of given complication.Conclusions. Analysis of values of parameters of hemostasis system in pregnant women with preeclampsia in the third trimester taking into account the risk factors and genetic predisposition has a prognostic value for the assessment of obstetric and perinatal risk and choice of tactics of conducting pregnancy and deliveryПроведен анализ результатов исследования показателей гемостаза у беременных с преэклампсией различной степени тяжести в третьем триместре. Установлено, что наиболее выраженное изменение величин показателей наблюдаются у беременных, имеющих факторы риска осложнений преэклампсии, ранние ее клинические проявления, генетический полиморфизм. Отмечены изменения величин показателей системы гемостаза в соответствии со степенью тяжести данного осложнени