Sistema de Gestión del Conocimiento ANLIS MALBRÁN
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[Electronic cardiovascular device-associated to infective endocarditis caused by Mycobacterium mageritense]
Get PDFRapidly growing non-tuberculosis mycobacteria are a rare cause of bacterial endocarditis. During the last decades, there has been an increase in infections due to rapidly growing mycobacteria, mainly after trauma and post-surgical procedures, both localized and disseminated, as well as nosocomial outbreaks due to contamination of medical equipment. Routine acid-fast staining for blood culture bottles is not always performed; however, the microbiologist should be aware of potential RGM infections especially when gram positive bacilli are observed. We describe a case of endocarditis caused by Mycobacterium mageritense in a patient with an autologous pericardial patch and a pressure catheter in the left auricle. The bacterial species was identified as Mycobacterium mageritense by mass spectrometry (MALDI-TOF MS), score 2.3, and confirmed by 16S rRNA analysis with 99.8 and 100% agreement, respectively
Impaired frequencies and function of platelets and tissue remodeling in chronic Chagas disease
Get PDFFil: Pengue, Claudia. Hospital Interzonal General de Agudos Eva Perón, Buenos Aires; Argentina.Fil: Cesar, Gonzalo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Alvarez, María Gabriela. Hospital Interzonal General de Agudos Eva Perón, Buenos Aires; Argentina.Fil: Bertocchi, Graciela. Hospital Interzonal General de Agudos Eva Perón, Buenos Aires; Argentina.Fil: Lococo, Bruno. Hospital Interzonal General de Agudos Eva Perón, Buenos Aires; Argentina.Fil: Viotti, Rodolfo. Hospital Interzonal General de Agudos Eva Perón, Buenos Aires; Argentina.Fil: Natale , María Ailén. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Castro Eiro, Melisa D.. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Cambiazzo , Silvia S.. Hospital General de Agudos Dr. Teodoro Álvarez, Buenos Aires; Argentina.Fil: Perroni, Nancy. Hospital Interzonal General de Agudos Eva Perón, Buenos Aires; Argentina.Fil: Nuñez, Myriam. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Matemática y Física, Buenos Aires; Argentina.Fil: Albareda, María Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Laucella, Susana A.. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Chronic inflammation, as a consequence of the persistent infection with Trypanosoma cruzi, leads to continuous activation of the immune system in patients with chronic Chagas disease. We have previously shown that increased sera levels of soluble P-selectin are associated with the severity of the cardiomyopathy distinctive of chronic Chagas disease. In this study, we explored the expression of biomarkers of platelet and endothelial activation, tissue remodeling, and mediators of the coagulation cascade in patients at different clinical stages of chronic Chagas heart disease. The frequencies of activated platelets, measured by the expression of CD41a and CD62P were decreased in patients with chronic Chagas disease compared with those in uninfected subjects, with an inverse association with disease severity. Platelet activation in response to adenosine diphosphate was also decreased in T. cruzi-infected subjects. A major proportion of T. cruzi infected subjects showed increased serum levels of fibrinogen. Patients with severe cardiac dysfunction showed increased levels of endothelin-1 and normal values of procollagen I. In conclusion, chronic infection with T. cruzi induced hemostatic alterations, even in those patients who do not yet present cardiac symptoms
Tamoxifen acts on Trypanosoma cruzi sphingolipid pathway triggering an apoptotic death process
No full textFil: Landoni, Malena. Universidad de Buenos Aires, FCEN, Departamento de Química Orgánica - CONICET; Argentina.Fil: Piñero, Tamara. Universidad de Buenos Aires, FCEN, Departamento de Química Orgánica - CONICET; Argentina.Fil: Soprano, Luciana L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Garcia-Bournissen, Facundo. Instituto Multidisciplinario de Investigaciones en Enfermedades Pedíatricas (IMIPP), CONICET, Hospital de Niños "Ricardo Gutiérrez"; Argentina.Fil: Fichera, Laura E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Esteva, Monica I. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Couto, Alicia S. Universidad de Buenos Aires, FCEN, Departamento de Química Orgánica - CONICET; Argentina.This study shows the effects of tamoxifen, a known estrogen receptor antagonist used in the treatment of breast cancer, on the sphingolipid pathway of Trypanosoma cruzi, searching for potential chemotherapeutic targets. A dose-dependent epimastigote growth inhibition at increasing concentration of tamoxifen was determined. In blood trypomastigotes, treatment with 10 μM showed 90% lysis, while 86% inhibition of intracellular amastigote development was obtained using 50 μM. Lipid extracts from treated and non-treated metabolically labelled epimastigotes evidenced by thin layer chromatography different levels of sphingolipids and MALDI-TOF mass spectrometry analysis assured the identity of the labelled species. Comparison by HPLC-ESI mass spectrometry of lipids, notably exhibited a dramatic increase in the level of ceramide in tamoxifen-treated parasites and a restrained increase of ceramide-1P and sphingosine, indicating that the drug is acting on the enzymes involved in the final breakdown of ceramide. The ultrastructural analysis of treated parasites revealed characteristic morphology of cells undergoing an apoptotic-like death process. Flow cytometry confirmed cell death by an apoptotic-like machinery indicating that tamoxifen triggers this process by acting on the parasitic sphingolipid pathway
Mycosis Due to Tropicoporus tropicalis (= Inonotus tropicalis) in a Domestic Dog
No full textFil: Hevia, Alejandra I. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Iachini, Ricardo. Instituto de Zoonosis “Luis Pasteur”, Buenos Aires; Argentina.Fil: Fernández, Julián. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Lazzari, Julieta. Veterinaria Azcuénaga, Buenos Aires; Argentina.Fil: Suárez-Alvarez, Roberto. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Abrantes, Ruben Antonio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Toranzo, Adriana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Refojo, Nicolás. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Canteros, Cristina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Mycelial basidiomycetes rarely produce mycoses in animals including humans. We report a case of a 9-year-old female mongrel dog with lesions in the prescapular lymph nodes. The histopathology of a lymph node sample showed flexuous septate hyphae, and a sterile mold grew in culture from that specimen. DNA sequencing of the ITS region allowed us to identify the fungus as Tropicoporus tropicalis. The dog was treated with itraconazole, but it was euthanized six months later due to an unfavorable clinical outcome. Tropicoporus tropicalis is an infrequent pathogen of pets, and the use of molecular tools is needed for its identification. Animal infections due to T. tropicalis were not previously been reported in Argentina
Use of whole genome sequencing for the molecular comparison of Neisseria gonorrhoeae isolates with decreased susceptibility to extended spectrum cephalosporins from two geographically different regions in America
No full textFil: Gianecini, Ricardo A. Public Health Ontario Laboratories, Toronto; Canada.Fil: Zittermann, Sandra. Public Health Ontario Laboratories, Toronto; Canada.Fil: Oviedo, Claudia . ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.Fil: Galas, Marcelo F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Ramon Pardo, Pilar. Pan American Health Organization/World Health Organization (PAHO/ WHO), Washington, DC; Estados Unidos.Fil: Allen, Vanessa G. Public Health Ontario Laboratories, Toronto; Canada.Fil: Galarza, Patricia G. Public Health Ontario Laboratories, Toronto; Canada.Fil: Melano, Roberto. Public Health Ontario Laboratories, Toronto; Canada.Neisseria gonorrhoeae isolates with reduced susceptibility or resistance to the recommended first-line antimicrobial therapy have been described in several countries. The purpose of this study was to use genome analyses to compare the molecular characteristics of N. gonorrhoeae isolates with decreased susceptibility to extended-spectrum cephalosporin (ESC) from Ontario, Canada, and Argentina
Genetics and genomic medicine in Argentina
No full textIn this letter, we want to add information to the paper "Genetics and genomic medicine in Argentina" that we considered it was lacking. Argentina is a big country with inequalities in the access to public health care, especially in medical genetics and genomics
Characterization of a multidrug resistant Citrobacter amalonaticus clinical isolate harboring blaNDM-1 and mcr-1.5 genes
No full textFil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.Fil: Albornoz, Ezequiel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.Fil: Tijet, Nathalie. Public Health Ontario Laboratory; Canada.Fil: Biondi, Estefania. Hospital de niños Dr. Ricardo Gutierrez; Argentina.Fil: Gómez, Sonia A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.Fil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.Fil: Vázquez, Miryam. Hospital de niños Dr. Ricardo Gutiérrez; Argentina.Fil: Melano, Roberto. Public Health Ontario Laboratory; Canada.Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.A multidrug resistant isolate, identified as Citrobacter amalonaticus using MALDI-TOF MS and confirmed by genomic analysis, was recovered from a pediatric patient in a hospital from Buenos Aires, Argentina. By whole-genome sequencing a total of 16 resistance genes were detected, including blaNDM-1 and mcr-1.5. To the best of our knowledge this is the first description of these two genes together in a clinical isolate of the Citrobacter genus
Germline pathogenic variants in BRCA1, BRCA2, PALB2 and RAD51C in breast cancer women from Argentina
No full textPurposeEach year, 17,000 new breast cancer cases are diagnosed in Argentina, and 5400 women die of breast cancer. The contribution of cancer-related mutations to the incidence of breast cancer in Argentina has not yet been explored.MethodsWe sequenced the entire coding regions of BRCA1, BRCA2, PALB2 and RAD51C in 112 unselected Argentinian breast cancer patients.ResultsA pathogenic genetic variant was found in 12 of 112 (10.7%) patients; two in BRCA1 (1.8%), five in BRCA2 (4.5%), four in PALB2 (3.6%) and one in RAD51C (0.9%). Three of four (75%) PALB2 mutation carriers carried the same variant (c.1653T > A).ConclusionsA founder mutation in PALB2 accounts for up to 4% of breast cancer patients in Argentina. BRCA1, BRCA2, PALB2 and RAD51C should be included in the genetic testing panel of breast cancer patients in Argentina
An Stx-EAEC O59:NM[H19] strain isolated from a hemolytic uremic syndrome case in Argentina
Get PDFFil: Carbonari, Claudia C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Ricciardi, Miriam. Hospital Zonal de Puerto Madryn "Dr. Andrés Ísola"; Chubut, Argentina.Fil: Calvo, Ana Rodríguez. Hospital Zonal de Puerto Madryn "Dr. Andrés Ísola"; Chubut, Argentina.Fil: Montes, Alejandro. Hospital Zonal de Puerto Madryn "Dr. Andrés Ísola"; Chubut, Argentina.Fil: Deza, Natalia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Conde Valentino, María A. Hospital Zonal de Puerto Madryn "Dr. Andrés Ísola"; Chubut, Argentina.Fil: Zolezzi, Gisela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Vago, Mariano. Hospital Zonal de Puerto Madryn "Dr. Andrés Ísola"; Chubut, Argentina.Fil: Acosta, Denise. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Manfredi, Eduardo A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Chinen, Isabel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Shiga toxin-producing Escherichia coli (STEC) are a heterogeneous group of foodborne pathogens causing a broad spectrum of human disease, from uncomplicated diarrhea to hemolytic uremic syndrome (HUS). In this study, we report an HUS case associated with an O59:NM[H19] strain, harboring stx2a, iha, lpfAO26, lpfAO113 genes associated with STEC, and aatA, aap, pic, sigA, agg4A genes associated with enteroaggregative E. coli (EAEC), named Stx-EAEC. The strain showed low toxicity on Vero cells, and was resistant to streptomycin and trimethoprim/sulfonamides. The child carried the bacteria for more than 100 days. Since the large outbreak associated with Stx-EAEC O104:H4, many strains with similar profiles have been described. In Germany, an O59:NM[H19] strain, with comparable characteristics to the Argentine strain, was isolated from a bloody diarrhea case. In Argentina, this is the first report of an HUS case associated with a Stx-EAEC infection, and represents a new challenge for the surveillance system
Acute disseminated encephalomyelitis due to Mycoplasma pneumoniae in a previously healthy boy
No full textFil: Cadario, María Estela. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI); Argentina.Fil: Ellis, Alejandro. Sanatorio Mater Dei. Servicio de pediatría; Argentina.Fil: Garea, Mónica. Sanatorio Mater Dei. Servicio de pediatría; Argentina.Fil: Cairnie, Antonio. Sanatorio Mater Dei. Servicio de pediatría; Argentina.Fil: Mistchenko, Alicia. Hospital de niños "Dr. Ricardo Gutiérrez". Servicio de virología; Argentina.Fil: García Roig, Cristian. Sanatorio Mater Dei. Servicio de pediatría; Argentina.Fil: Freire, María Cecilia. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI); Argentina.Fil: Savy, Vilma. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI); Argentina.Fil: Sciarrotta, Juan Antonio. Sanatorio Mater Dei. Servicio de pediatría; Argentina.We present here the case of a previously healthy 5 year-old boy hospitalized in an intensive care unit due to tonic-clonic seizures focused on the face and right side of the body, and axillary temperature of 37.4°C. Common bacterial and viral etiology was ruled out through studies of cerebrospinal fluid (CSF) samples. Mycoplasma pneumoniae was suspected by a positive immunofluorescence serum test for IgM class antibodies. Finally, with a brain biopsy, M. pneumoniae was confirmed by polymerase chain reaction (PCR) and acute disseminated encephalomyelitis by pathological anatomy. The patient was treated with clarithromycin and had an uneventful evolution. At least to our knowledge, this is the first case in which M. pneumoniae DNA was detected by PCR in a brain biopsy