RUNA - Repositorio de Saúde
Not a member yet
11570 research outputs found
Sort by
Vesicles Shed by Pathological Murine Adipocytes Spread Pathology: Characterization and Functional Role of Insulin Resistant/Hypertrophied Adiposomes
Get PDFExtracellular vesicles (EVs) have recently emerged as a relevant way of cell to cell communication, and its analysis has become an indirect approach to assess the cell/tissue of origin status. However, the knowledge about their nature and role on metabolic diseases is still very scarce. We have established an insulin resistant (IR) and two lipid (palmitic/oleic) hypertrophied adipocyte cell models to isolate EVs to perform a protein cargo qualitative and quantitative Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH) analysis by mass spectrometry. Our results show a high proportion of obesity and IR-related proteins in pathological EVs; thus, we propose a panel of potential obese adipose tissue EV-biomarkers. Among those, lipid hypertrophied vesicles are characterized by ceruloplasmin, mimecan, and perilipin 1 adipokines, and those from the IR by the striking presence of the adiposity and IR related transforming growth factor-beta-induced protein ig-h3 (TFGBI). Interestingly, functional assays show that IR and hypertrophied adipocytes induce differentiation/hypertrophy and IR in healthy adipocytes through secreted EVs. Finally, we demonstrate that lipid atrophied adipocytes shed EVs promote macrophage inflammation by stimulating IL-6 and TNFalpha expression. Thus, we conclude that pathological adipocytes release vesicles containing representative protein cargo of the cell of origin that are able to induce metabolic alterations on healthy cells probably exacerbating the disease once established
Development and validation of a clinical score to estimate progression to severe or critical state in Covid-19 pneumonia hospitalized patients
Get PDFThe prognosis of a patient with Covid-19 pneumonia is uncertain. Our objective was to establish a predictive model of disease progression to facilitate early decision-making.
A retrospective study was performed of patients admitted with Covid-19 pneumonia, classified as severe (admission to the intensive care unit, mechanic invasive ventilation, or death) or non-severe. A predictive model based on clinical, analytical, and radiological parameters was built. The probability of progression to severe disease was estimated by logistic regression analysis. Calibration and discrimination (receiver operating characteristics curves and AUC) were assessed to determine model performance.
During the study period 1,152 patients presented with Covid-19 infection, of whom 229 (19.9%) were admitted for pneumonia. During hospitalization, 51 (22.3%) progressed to severe disease, of whom 26 required ICU care (11.4); 17 (7.4%) underwent invasive mechanical ventilation, and 32 (14%) died of any cause. Five predictors determined within 24 hours of admission were identified: Diabetes, Age, Lymphocyte count, SaO2, and pH (DALSH score). The prediction model showed a good clinical performance, including discrimination (AUC 0.87 CI 0.81, 0.92) and calibration (Brier score = 0.11). In total, 0%, 12%, and 50% of patients with severity risk scores ≤5%, 6-25%, and >25% exhibited disease progression, respectively.
A simple risk score based on five factors predicts disease progression and facilitates early decision-making according to prognosis.Carlos III Health Institute, Spain, Ministry of Economy and Competitiveness (SPAIN) and the European Regional Development Fund (FEDER)Instituto de Salud Carlos II
Alcohol : non te deixes enganar
Get PDFGuía con información para a prevención do consumo de alcoholGuía con información para la prevención del consumo de alcoho
Protocolo de manexo de diverticulite aguda. PT-XIF-CLN-CLN-122/00
No full textO obxectivo deste documento é establecer un protocolo consensuado para o diagnóstico, clasificación, tratamento e seguimento dos pacientes da Área Sanitaria de Ferrol con diverticulite aguda.El objetivo de este documento es establecer un protocolo consensuado para el diagnóstico, clasificación, tratamiento y seguimiento de los pacientes del Área Sanitaria de Ferrol con diverticulitis aguda
Cómo realizar una búsqueda sencilla en el nuevo Pubmed: vídeo tutorial
No full textVídeo tutorial realizado por Bibliosaúde de apoyo a los cursos online, para realizar una búsqueda simple en el nuevo Pubmed. El fin es explicar el funcionamiento de la nueva interfaz de Pubmed y no cómo realizar una estrategia de búsquedaVídeo titorial realizado por Bibliosaúde de apoio aos cursos en liña, para realizar unha procura simple no novo Pubmed. O fin é explicar o funcionamento da nova interface de Pubmed e non como realizar unha estratexia de procur
Profilaxe pre-exposición (PrEP) ao VIH en Galicia
Get PDFA profilaxe pre-exposición (PrEP) é unha intervención biomédica dirixida a previr a transmisión da infección polo virus da inmunodeficiencia humana (VIH) en persoas seronegativas para o VIH que teñen un elevado risco de contraer a infección. Consiste na administración de Tenofovir disoproxil fumarato (TDF) e Emtricitabina (FTC), antes da exposición ao VIH.La profilaxis pre-exposición (PrEP) es una intervención biomédica dirigida a prevenir la transmisión de la infección por virus de la inmunodeficiencia humana (VIH) en personas seronegativas para el VIH que tienen un elevado riesgo de contraer la infección. Consiste en la administración de Tenofovir disoproxil fumarato (TDF) y Emtricitabina (FTC), antes de la exposición al VIH
Axudas, consellos e accións para deixar de fumar. Chegou o momento. Xa NON fumo!
Get PDFPublicación na que se recolle unha serie de información sobre axudas, consellos e tarefas a realizar que poden axudar ás persoas que deixaron de fumar recentemente para manter a súa abstinencia.Publicación en la que se recoge una serie de información sobre ayudas, consejos y tareas a realizar que pueden ayudar a las personas que dejaron de fumar recientemente para mantener su abstinencia
Guía de saúde infantil : actividades preventivas e de promoción da saúde en pediatría de atención primaria
Get PDFSe trata de una guía de salud infantil dirigida a los profesionales que trabajan en el ámbito de la asistencia sanitaria a los niños y adolescentes en atención primaria del Sistema Público de Salud de Galicia.Trátase dunha guía de saúde infantil dirixida aos profesionais que traballan no ámbito da asistencia sanitaria aos nenos e adolescentes na atención primaria do Sistema Público de Saúde de Galicia
Bibliosaúde Memoria 2019
No full textMemoria anual de Bibliosaúde del año 2019Memoria anual de Bibliosaúde do ano 201
Campaña de vacinación antigripal 2020. Instrución 20/20
No full textInstrución reguladorada campaña de vacinación antigripal 2020, con aspectos técnicos, loxísticos e de xestión para a información aos profesionais sanitarios e á cidadaníaInstrucción reguladorada de la campaña de vacunación antigripal 2020, con aspectos técnicos, logísticos y de gestión para la información a los profesionales sanitarios y a la ciudadaní