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Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines
No full textInternational audienceSodium (Na+) ions are known to regulate many signaling pathways involved in both physiological and pathological conditions. In particular, alterations in intracellular concentrations of Na+ and corresponding changes in membrane potential are known to be major actors of cancer progression to metastatic phenotype. Though the functionality of Na+ channels and the corresponding Na+ currents can be investigated using the patch-clamp technique, the latter is rather invasive and a technically difficult method to study intracellular Na+ transients compared to Na+ fluorescence imaging. Despite the fact that Na+ signaling is considered an important controller of cancer progression, only few data using Na+ imaging approaches are available so far, suggesting the persisting challenge within the scientific community. In this study, we describe in detail the approach for application of Na+ imaging technique to measure intracellular Na+ variations in human prostate cancer cells. Accordingly, we used three Na+-specific fluorescent dyes-Na+-binding benzofuran isophthalate (SBFI), CoroNa™ Green (Corona) and Asante NaTRIUM Green-2 (ANG-2). These dyes have been assessed for optimal loading conditions, dissociation constant and working range after different calibration methods, and intracellular Na+ sensitivity, in order to determine which probe can be considered as the most reliable to visualize Na+ fluctuations in vitro
Predictive spatio-temporal model for spatially sparse global solar radiation data
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Lag-generalized synchronization of time-delay chaotic systems with stochastic perturbation
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L'intérêt du modèle d'interaction aptitude-traitement pour mieux comprendre les processus associés aux traits de personnalité : le cas de la disposition à l'évitement expérientiel
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Robotique à l'école primaire, quelles aides outillées et pour quelles activités ?
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An adaptive actuator failure compensation scheme for two linked 2WD mobile robots.
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Physical and Cognitive Stimulation Using an Exergame in Subjects with Normal Aging, Mild and Moderate Cognitive Impairment
No full textInternational audienceThe use of Serious exerGames (SeG) as enriched environments (EE), which promotes cognitive simulation with physical activity in a positive emotional context, has been proposed to represent a powerful method to slow down the decline due to neurodegenerative diseases (ND), such as Alzheimer's disease (AD). However, so far, no SeG targeting EE has been tested in ND subjects
Automatic Shear Wave Velocity Estimation in Bender Element Testing
No full textInternational audienceDynamic properties of soils, such as shear wave velocity and damping, are some of critical parameters in the design of civil infrastructures subjected to vibrations from earthquakes and machine foundations. Bender element (BE) testing is used in the laboratory to determine shear wave velocity; however, there is no standard procedure for the interpretation of the results. The main limitation in BE testing is the correct estimation of the shear wave arrival time leading to uncertainty in the values of shear wave velocity. In this paper, three different automatic methods for the shear wave arrival time detection were tested. From these methods, a new procedure was proposed for the automatic determination of the shear wave arrival time using the Akaike information criterion. The method is tested on a series of bender element measurements giving less than 5 % error when the results were compared with the standard method for a signal-to-noise ratio higher than four