Green Medical Journal
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Sensitive Molecular Detection for Salmonella Enterica Serovars Typhimurium
No full textIntroduction: Salmonella infections contribute significantly to gastroenteritis cases, with the National Salmonella Reference Laboratory reporting 500 isolates in 2022. However, traditional culture-based methods for detecting Salmonella in samples can take 4 to 7 days to confirm a positive result, which poses health risks due to delayed detection. Given these health risks, swift and accurate detection methods are essential to minimize both false-positive and false-negative outcomes. Salmonella, a gram-negative bacterium within the Enterobacteriaceae family, demonstrates remarkable hardiness, surviving for several weeks in dry environments and months in water. Although most serotypes of Salmonella cause relatively mild gastroenteritis, some, particularly those transmitted from animals to humans, can lead to severe, life-threatening conditions
Methods: The qRT-PCR procedure involved the design of primers and probes targeting the same genes as the mPCR assay. These primer sets were reconfigured to generate smaller amplicons suitable for qRT-PCR systems
Results: qRT-PCR process, TaqMan probes were meticulously designed for specific target genes: FAM dye was employed to detect STM2745, Cy5 dye was used for STM4492, and Rox dye was utilized to detect. A standard curve was constructed using Typhimurium LT2 genomic DNA. Each sample underwent duplicate analysis, and Rotor-Gene software was employed to assign threshold values for each channel.
Conclusion: The effectiveness of our qPCR assay for the detection of Salmonella across a diverse array of matrices. Notably, our results unveiled distinct limits of detection for Salmonella in various samples. Specifically, a parallel vein, the deployment of a PCR assay, leveraging an immunomagnetic separation technique for DNA extraction, was studied by another group. While subsequent analysis of Salmonella detected via our assay may necessitate the full ISO SMT method for live culture isolation, this supplementary step can be seamlessly conducted alongside qRT-PCR
Event of Nasopharyngeal Carcinoma in Coastal Areas: Review Article
Full text linkBackground: Indonesia is a country consisting of islands and oceans that are rich in potential natural resources. Abundant marine products make the Indonesian people empower fish catches into long-lasting salted fish processed foods. People living in coastal areas have a high level of consumption of salted seafood products, such as salted fish. Consuming salted fish is one of the factors causing the incidence of nasopharyngeal carcinoma.
Contents: Nasopharyngeal carcinoma (NPC) is a type of cancer found in the head and neck. NPC cases can be caused by various factors, one of which is caused by environmental factors. Environmental factors that can cause the incidence of NPC are environments that have a population of both passive and active smokers, formaldehyde exposure, and consumption of salted fish. Clinical manifestations that can arise in patients with NPC, such as epistaxis, nasal congestion, ringing in the ears, headaches, facial pain, and lumps can be seen on the top of the neck of the patient.
Conclusion: NPC is the fourth most common malignant tumor in Indonesia after cervical cancer, breast cancer, and skin cancer. NPC cases are most commonly found in men compared to women in the elderly age group, in the range of 45-55 years. Several treatments such as radiotherapy, chemotherapy, and surgical intervention can be done to treat NPC cases.
 
Occupational Noise Induced Hearing Loss in Motor Boat Fisherman
Full text linkIntroduction: Noise Induced Hearing Loss is a person's hearing loss either partially or completely which is permanent and can affect one or both ears caused by continuous exposure to noise in the work environment. One of the causes of Noise Induced Hearing Loss (NIHL) is working as a motorboat fisherman.
Content: NIHL can arise as a result of the interaction between genetic factors and environmental factors with the role of internal and external risk factors. The diagnosis of NIHL can be established through history taking, physical examination such as otoscopy and tuning fork tests, as well as supporting examinations in the form of pure tone audiometry.
Conclusion: NIHL will produce the impression of sensorineural deafness with a permanent nature so that the only treatment that can be given is supportive and preventive management.
Keywords: Noisy; noise-induced hearing loss; motorboat fisherma
Brain Tumor in Pediatrics: Pediactric Glioblastoma: Review Article
No full textBackground: Glioblastoma (GBM) is a malignant brain tumor that can occur in children and is very aggressive which means it can grow and spread quickly. Approximately 16% of all primary brain and central nervous system neoplasms are glioblastomas. This makes GBM a major public health problem. The incidence of glioblastoma in children varies.glioblastoma Low-grade has a higher incidence in children aged 0 to 14 years, which is 1.8/100,000 while high-grade glioblastoma is 0.5/100,000 lower than low-grade glioblastoma. where the incidence rate of glial and neuroepithelial tumors is higher in children aged 0-14 years.
Content: Various factors such as genetic and environmental factors have been studied in terms of the etiology of glioblastoma. GBM develops more frequently in children with specific genetic syndromes, such as neurofibromatosis 1 (NF1), Li-Fraumeni syndrome, hereditary nonpolyposis colon cancer, and tuberous sclerosis. However, most cases of GBM have no known cause. Symptoms of glioblastoma are non-specific. The duration of symptoms is usually short and lasts for several months
Summary: In children, central nervous system tumors are the most common, with 3-15% being glioblastomas. Glioblastoma usually presents with non-specific symptoms and lasts a short duration of several months. In addition, the prognosis is also poor with high morbidity and mortality so that to improve the patient's quality of life, effective therapy is needed. Therapy can be in the form of surgery, radiotherapy, or chemotherapy
Risk Factors Typhoid Fever Incidence at Lau Health Center, Lau District, Maros Regency in 2021
No full textBackground: Typhoid fever is disease caused by the bacterium Salmonella typhi genus Salmonella. Until now, disease fever typhoid still is problem health in tropical countries including Indonesia. Knowing risk factor incident fever Typhoid at the Lau Public Health Center, Lau district, Maros regency in 2021
Method: Research type analytic observational with case control design. The data analysis technique uses the chi-square statistical test.
Results: Typhoid Fever mostly found in the age 11-19 years as many as 21 people ( P-value 0.049), gender obtained that the most in men as many as 22 people ( P-value 0.014), level primary school education is obtained as many as 14 people ( P-value 0.07), habit wash hand before eat less good obtained as many as 17 people ( P-value 0.044), habit wash hand after less CHAPTER good obtained as many as 19 people ( P-value 0.013), habit eat / snack outside house obtained as many as 23 people ( P-value 0.026), habit wash ingredients food less raw good obtained as many as 18 people ( P-value 0.046) and sanitation less environment good obtained as many as 25 people P-value 0.041) .
Conclusion: Based on results study concluded that on the variable age , gender , education , habits wash hand before eat , habit wash hand after defecation, habit eat / snack outside home , custom wash ingredients food raw and sanitary environment own connection with factor risk incident fever typhoid in the health center lau districts lau districts maros year 2021
Risk Factor Analysis of Anxiety Disorder in Third Trimester Pregnant Women
Full text linkIntroduction: Pregnancy is a natural thing for productive women. Anxiety that occurs in pregnancy is an emotional response related to the concern felt by the mother for the well-being of herself and her fetus during pregnancy, childbirth and the role of being a mother later. The purpose of this study was to determine the relationship between age, level of education, occupation, economy, parity, environmental status, and religious spiritual activities on anxiety disorders in third trimester pregnant women in Somba Opu District, Gowa Regency.
Methods: The instrument in this study used a questionnaire. The sample used in this study consisted of 136 third trimester pregnant women in the work area of Syekh Yusuf Hospital and Sungguminasa Health Center. Data was processed and analyzed as needed and data analysis using Chi Square test p<0.05
Results: The study showed that there was a significant relationship between age, education level, occupation, economic factors, parity, and religious spiritual activities with anxiety disorder levels in third trimester pregnant women and this study did not show that there was a significant relationship between living environment status. with the level of anxiety disorder in third trimester pregnant women (0.514)
Conclusion: There is a relationship between age, education level, occupation, economic factors, parity, and religious spiritual activities with anxiety disorder levels in third trimester pregnant women, and there is no relationship between living environment status and anxiety disorder levels in third trimester pregnant women
Komplikasi Pada Diabetes Melitus : Tinjauan Pustaka
No full textIntroduction: Diabetes Mellitus is a chronic disease that will be suffered for a lifetime so the progressive disease will continue and can cause complications. Diabetes mellitus usually progresses slowly with symptoms that are mild to severe and can even cause death due to acute or chronic complications.
Content: Hyperglycemic crisis is one of the acute complications that can occur in Diabetes mellitus, both type 1 and type 2. This situation is a serious complication that may occur even in well-controlled diabetes mellitus.
Conclusion: Diabetic ketoacidosis and hyperglycemic Hyperosmolar State are acute complications of Diabetes mellitus. Both of these complications can be triggered due to inadequate insulin therapy and the presence of infection. Diabetic ketoacidosis is a condition characterized by metabolic acidosis due to excessive formation of ketones, while a hyperglycemic hyperosmolar state is characterized by severe hyperosmolality with serum glucose levels which are usually higher than diabetic ketoacidosis
Hubungan Antara Perokok Pasif Pada Kehamilan Dengan Bayi Berat Lahir Rendah di RSUD H. Padjonga Dg. Ngalle Takalar Tahun 2019
No full textIntroduction: A newborn who weighs less than 2,500 grams at delivery is considered low birth weight (LBW). There are many factors that can influence the determinants of LBW. One of the multifactorial is pre-natal exposure to tobacco smoke from the partner or environment in pregnant women (passive smokers) which provides negative effects for both the mother and the fetus. This study aims to determine the relationship between passive smoking during pregnancy and LBW at RSUD H. PadjonganDg. Ngalle Takalar in 2019.
Methods: The research is an observational analysis with a case-control approach. The population in this study is all mothers who gave birth to babies in the delivery room at RSUD H. PadjonganDg. Ngalle Takalar had 139 mothers who gave birth from January to December 2019. The sampling technique in this study used purposive sampling
Results: The results showed the pregnant woman's environment who was most often exposed to cigarette smoke with LBW (<0.05), Cigarette smoke exposure in the home with LBW (<0.05), Cigarette smoke exposure in the outdoor environment with LBW (<0.05), Pregnant women who were exposed to cigarette smoke in one week with LBW(<0.05), Frequency of time pregnant women were exposed to cigarette smoke in one day with LBW(<0.05).
Conclusion: There is a significant relationship between passive smoking during pregnancy and low birth weight (LBW).
Keywords: Passive smoking; pregnancy; low birth weightPendahuluan: Berat lahir rendah (BBLR) adalah berat bayi saat lahir kurang dari 2.500 gram. Ada banyak faktor yang dapat mempengaruhi penentu BBLR. Salah satu faktor multifaktorial adalah paparan pre-natal terhadap asap tembakau dari pasangan atau lingkungan pada ibu hamil (perokok pasif) yang memberikan dampak negatif bagi ibu dan janin. Penelitian ini bertujuan untuk mengetahui hubungan antara perokok pasif selama kehamilan dan BBLR di RSUD H. PadjonganDg. Ngalle Takalar pada tahun 2019.
Metode: Penelitian ini merupakan penelitian korelasional deskriptif dengan desain pendekatan retrospektif. Populasi dalam penelitian ini adalah semua ibu yang melahirkan bayi di ruang bersalin RSUD H. PadjonganDg. Ngalle Takalar dengan total 139 ibu yang melahirkan dari bulan Januari hingga Desember 2019. Teknik sampling pada penelitian ini menggunakan metode accidental sampling, dengan total sampel 60 orang dan hasil diolah menggunakan SPSS dengan uji Chi square.
Hasil: Hasil penelitian menunjukkan lingkungan ibu hamil yang paling sering terpapar asap rokok dengan BBLR (<0,05), Paparan asap rokok di rumah dengan BBLR (<0,05), Paparan asap rokok di lingkungan luar rumah dengan BBLR (<0,05), Ibu hamil yang terpapar asap rokok dalam satu minggu dengan BBLR (<0,05), Frekuensi waktu ibu hamil terpapar asap rokok dalam satu hari dengan BBLR (<0,05).
Kesimpulan: Ada hubungan yang signifikan antara perokok pasif selama kehamilan dan berat lahir rendah (BBLR)
Mckenzie Method for Chronic Low Back Pain: Review
Full text linkBackground: Chronic low back pain (CLBP) is one of the most prevalent and expensive musculoskeletal issues in contemporary culture. It is defined as low back pain that lasts for more than three months or longer than the anticipated recovery period.[1] 70 to 80 percent of adults have dealt with CLBP at some point in their lives. This study sought to assess the McKenzie method's contribution to the treatment of low back pain and determine whether it could be compared to other forms of therapy.
Content: A narrative review of the literature was performed using the following search engines: PubMed, Cochrane, and Scopus. Inclusion criteria were articles published in the last 10 years, randomized clinical trials, the mean age of patients between 18 and 70 years and full English text. Exclusion criteria were observational studies, case reports and articles without abstract or full text. The outcomes that were used to review included chronic pain, disability, Quality of Life (QoL) and psychological aspects.
Conclusion: Even though it has helped treat patients with chronic low back pain, the McKenzie method needs more research to be proven effective in particular patient populations.
Keywords: Chronic low back pain; McKenzie method; Human
Gastroduodenitis kronik pada anak: laporan kasus
No full textRecurrent abdominal pain is the most common symptom experienced by children around the world and causes high rates of children absent from school. Gastroduodenitis is a disease with abdominal pain symptoms. The initial clinical symptom was dyspepsia. In most pediatric cases the etiology is unknown (idiopathic). This study presented a case of gastroduodenitis in a 10-year-old boy hospitalized with the chief complaints of heartburn, accompanied by vomiting. The patient has a history of the same complaint in the last 3 months ago, causing the patient to be admitted to the hospital repeatedly. Pain is not relieved by defecation or flatus. On physical examination, tenderness was found in the epigastric and umbilical regions. Chronic gastroduodenitis was confirmed after endoscopic examination and tissue biopsy. Therapy including proton pump inhibitors and lifestyle modifications can improve the child's condition. In the case of gastroduodenitis, it is important to prevent complications, so proper examination and prompt treatment are needed for the sufferer