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    High-Throughput Phenotyping Approach for Screening Major Carotenoids of Tomato by Handheld Raman Spectroscopy Using Chemometric Methods

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    Our objective was to develop a rapid technique for the non-invasive profiling and quantification of major tomato carotenoids using handheld Raman spectroscopy combined with pattern recognition techniques. A total of 106 samples with varying carotenoid profiles were provided by the Ohio State University Tomato Breeding and Genetics program and Lipman Family Farms (Naples, FL, USA). Non-destructive measurement from the surface of tomatoes was performed by a handheld Raman spectrometer equipped with a 1064 nm excitation laser, and data analysis was performed using soft independent modelling of class analogy (SIMCA)), artificial neural network (ANN), and partial least squares regression (PLSR) for classification and quantification purposes. High-performance liquid chromatography (HPLC) and UV/visible spectrophotometry were used for profiling and quantification of major carotenoids. Seven groups were identified based on their carotenoid profile, and supervised classification by SIMCA and ANN clustered samples with 93\% and 100\% accuracy based on a validation test data, respectively. All-trans-lycopene and beta-carotene levels were measured with a UV-visible spectrophotometer, and prediction models were developed using PLSR and ANN. Regression models developed with Raman spectra provided excellent prediction performance by ANN (r(pre)= 0.9, SEP = 1.1 mg/100 g) and PLSR (r(pre)= 0.87, SEP = 2.4 mg/100 g) for non-invasive determination of all-trans-lycopene in fruits. Although the number of samples were limited for beta-carotene quantification, PLSR modeling showed promising results (r(cv)= 0.99, SECV = 0.28 mg/100 g). Non-destructive evaluation of tomato carotenoids can be useful for tomato breeders as a simple and rapid tool for developing new varieties with novel profiles and for separating orange varieties with distinct carotenoids (high in beta-carotene and high incis-lycopene)

    Nearly Zero Energy Buıldıng Desıgn And Comparıson

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    Dünyadaki enerji ihtiyacı her geçen gün artarak devam etmektedir. Bu enerji ihtiyacının büyük bölümünü binalar oluşturmaktadır. Avrupa Birliği’nde binaların enerji tüketimindeki payı %40’tır. Benzer şekilde Türkiye’de enerji tüketiminde en fazla pay binalara aittir. Bu büyük paydan tasarruf elde edebilmek için Avrupa Birliği tarafından çeşitli çalışmalar yürütülmektedir. Avrupa Birliği binalarda enerji tüketiminin ölçülmesi ve bilinçli yönetilmesi hedefi ile 2002/91/EC sayılı “Binaların Enerji Performansı Direktifi”’ni 4 Ocak 2003 tarihinde yürürlüğe koymuş ve 19 Mayıs 2010 tarih ve 2010/31/EU sayı ile yeniden şekillendirmiştir. Avrupa Birliği’ne uzun yıllardır aday ülke konumundaki Türkiye’de bu gelişmelere paralel olarak 5627 sayılı Enerji Verimliliği Kanunu’na dayalı “Binalarda Enerji Performansı Yönetmeliği” 5 Aralık 2009 tarihinde yürürlüğe girmiştir. Ayrıca; enerji tüketim değerlerinin azaltılması doğrultusunda yapılan çalışmalar desteklenmekte ve yaygınlaştırılmaya çalışılmaktadır. Bu gelişmeler doğrultusunda, Avrupa Birliği ülkelerinde bu konuda yapılan örnek çalışmalar ve üye ülkelerin mevzuatları incelenmiştir. Türkiye’de Malatya ilinde kullanılan ve konvansiyonel yöntemler ile inşa edilmiş bir kamu hizmet binasının sıfıra yakın enerjili olması durumunun mevcut durumu ile karşılaştırılması yapılmıştır. Kamu hizmet binasının EnergyPlus programı ve program ara yüzü olan DesignBuilder ile modellemesi proje bilgilerinden faydalanılarak oluşturulmuş ve kayıtlı tüketim verileri sisteme girilerek simülasyonu yapılmıştır. Daha sonra, oluşturulan baz modelin sıfıra yakın enerjili bina olması durumu Avrupa Birliği’nde bulunan örnek binaların yapı elemanlarının ortalama ısı iletim katsayıları kullanılarak simüle edilmiş ve iki durum karşılaştırılmıştır. Elde edilen sonuçlarla ekonomiklik açısından Türkiye’de binaların sıfıra yakın enerjili bina standartlarına getirilebilmesi ve yaygınlaştırılabilmesi için devlet teşviklerinin artırılması ve Avrupa Birliği fonları gibi fonlardan örnek çalışmalar ile yararlanılabilmesi gerektiği sonucuna ulaşılmıştır.The energy need of the earth increasingly goes on day by day. Buildings generate the majority of the energy need. The rate of buildings in energy consumption is 40% in the European Union. Similarly, the most rate in energy consumption belongs to the buildings in Turkey. To get save on this great rate, various works are being carried out by the European Union. To be measured and consciously managed of energy consumption, European Union put in the force to “The Energy Directive of Buildings” number 2002/91/EC on 4th January 2003 and updated it with the date of 19th May 2010 and number 2010/31/EU. In Turkey, which is at the position of candidate country to European Union, “Regulation on Energy Performance in the Buildings’’ came into force in parallel with these developments on 5th December 2009. In line with these developments, the studies in the European Union countries and the legislation of the member countries have been examined. The public building in Malatya, which was built by conventional methods, was compared with the being able to nearly zero energy building. The public building modeling was made up of the EnergyPlus program and the Design Builder, which is a program interface, taking account of current situation values. Moreover, the situation of being able to nearly zero energy building of base model made was simulated by using average heat transformation coefficients of building elements of nearly zero energy buildings available in the European Union and two situations were compared. For the buildings being brought into nearly zero-energy buildings standards and popularized in Turkey, it was concluded that government promotions need to be increased and example works need to be benefited from funds such as European Union funds

    COVID-19 is a Real Headache!

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    After the emergence of a novel coronavirus named SARS-CoV-2, coronavirus disease 2019 (COVID-19) was initially characterized by fever, sore throat, cough, and dyspnea, mainly manifestations of respiratory system. However, other manifestations such as headache, abdominal pain, diarrhea, loss of taste and smell were added to the clinical spectrum, during the course of the COVID-19 pandemic. The reports on the neurological findings are increasing rapidly and headache seems to be the leader on the symptom list. Headache was reported in 11\%-34\% of the hospitalized COVID-19 patients, but clinical features of these headaches were totally missing in available publications. According to our initial experience, significant features of headache presentation in the symptomatic COVID-19 patients were new-onset, moderate-severe, bilateral headache with pulsating or pressing quality in the temporoparietal, forehead or periorbital region. The most striking features of the headache were sudden to gradual onset and poor response to common analgesics, or high relapse rate, that was limited to the active phase of the COVID-19. Symptomatic COVID-19 patients, around 6\%-10\%, also reported headache as a presenting symptom. The possible pathophysiological mechanisms of headache include activation of peripheral trigeminal nerve endings by the SARS-CoV-2 directly or through the vasculopathy and/or increased circulating pro-inflammatory cytokines and hypoxia. We concluded that as a common non-respiratory symptom of COVID-19, headache should not be overlooked, and its characteristics should be recorded with scrutiny

    Partial and full-thickness rotator cuff tears in patients younger than 45 years

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    Objective: The aim of this study was to evaluate the results of the arthroscopic repair in patients with partial and full thickness rotator cuff tears and less than 45 years of age. Methods: Fifty patients (26 women and 24 men; mean age: 41.4 +/- 3.96 years; range: 31-45) with rotator cuff tear, and who were treated with the arthroscopic repair, were included in the study. Twenty patients had full thickness and 30 had partial-thickness tears. The final functional evaluation was conducted at a mean of 42.4 months (range, 24 to 95 months; SD:13.3). The American Shoulder and Elbow Surgeon (ASES) self-report score and the University of California at Los Angeles Shoulder Score (UCLA Shoulder Score) were used as validated scoring systems. Results: At the final follow-up, the mean ASES and UCLA scores improved significantly to 72.3 and 26.5, respectively, in the full-thickness group (p<0.01). The mean ASES and UCLA scores improved significantly to 70.7 and 25.3, respectively, in the bursal-side group (p<0.01). The mean ASES and UCLA scores improved significantly to 75.3 and 27.1, respectively, in the joint-side group (p<0.01). There were no significant differences between the groups according to the postoperative ASES score (p>0.06) and UCLA score (p<0.37). Conclusion: The arthroscopic repair of the joint-sided tears and bursal-sided tears has good functional outcomes as full thickness rotator cuff tears, and the surgical option should be considered in younger population if the conservative treatment fails

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