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Detection of retinitis pigmentosa stages with GAN and transfer learning in maps of MfERG P1 wave amplitudes
No full text#GAMEADDICTED: A Machine Learning Framework for Digital Game Addiction Detection, and Early Intervention
No full textAntiproliferative and apoptotic effects of Pervari honey on SH-SY5Y neuroblastoma cells
No full textNeuroblastoma is a complex and heterogeneous pediatric malignancy. Treatment options for neuroblastoma include surgery, chemotherapy, radiotherapy, autologous stem cell transplantation, and emerging immunotherapies. However, these approaches often coincide with oxidative stress, highlighting the potential role of natural antioxidants in chemoprevention and chemotherapy. Honey has long been recognized for its medicinal properties, including antimicrobial, anti-inflammatory, and antioxidant effects. Pervari honey (PH), produced by bees that forage on a diverse array of native flora, is a distinctive honey originating from southeastern T & uuml;rkiye. This study evaluated the antiproliferative, anti-tumor, apoptotic, and inflammatory effects of PH on SH-SY5Y human neuroblastoma cells. Cell viability was measured using an MTT assay, while apoptosis was evaluated using an Annexin V-FITC/7-AAD staining test. The RT-qPCR method was used to quantify the expression of IL-1 beta, IL-6, TNF-alpha, NF-kappa B, Caspase-3, MMP-2, MMP-9, BAX, and BCL-2 genes. PH significantly reduced SH-SY5Y cell viability in a dose- and time-dependent manner (p < 0.05-p < 0.01). It induced apoptosis by upregulating BAX and Caspase-3 while downregulating BCL-2 (p < 0.05-p < 0.001). Additionally, it increased NF-kappa B expression (p < 0.05-p < 0.001) and modulated inflammatory (IL-1 beta, IL-6, and TNF-alpha) cytokines (p < 0.05-p < 0.01). MMP-2 and MMP-9 levels were significantly elevated (p < 0.05-p < 0.001), suggesting enhanced extracellular matrix remodeling. These in vitro findings indicate that PH may exert anti-tumor potential and possess immunomodulatory properties. Furthermore, in vitro and in vivo studies are needed to explore its therapeutic applicability
ANTARKTİKA LİKENİ RUSAVSKIA ELEGANS (LINK) S.Y. KONDR. & KÄRNEFELT TÜRÜNDEN ELDE EDİLEN METANOLİK ÖZÜTÜNÜN ANTİBAKTERİYEL AKTİVİTESİNİN DEĞERLENDİRİLMESİ
No full textBu çalışmada, Antarktika’da geniş yayılış gösteren likenleşmiş mantar Rusavskia elegans (Link) S.Y. Kondr. & Kärnefelt türünden elde edilen metanolik özütün antimikrobiyal etkileri detaylı olarak incelenmiştir. Antarktika, zorlu çevresel koşulları nedeniyle ekstremofilik mikroorganizmaların ve bunlarla simbiyotik ilişkiler kuran likenlerin biyolojik çeşitliliği açısından benzersiz bir ekosistem sunmaktadır.Araştırmada Rusavskia elegans’tan hazırlanan özütün, 2 Gram pozitif (Staphylococcus aureus,Bacillus subtilis), 2 Gram negatif (Escherichia coli, Enterobacter aerogenes) bakteri ve 2 maya(Candida albicans, Candida parapsilosis) kültürü üzerindeki antibakteriyel ve antifungal aktiviteleri agar kuyucuk difüzyon yöntemiyle değerlendirilmiştir. Elde edilen sonuçlar, özütün özellikle Enterobacter aerogenes (22 mm) ve Staphylococcus aureus (19 mm) bakterilerinde belirgin inhibisyon zonları oluşturduğunu, ayrıca maya türleri üzerinde (Candida albicans: 26 mm, C.parapsilosis: 24 mm) yüksek düzeyde antifungal etkiye sahip olduğunu göstermiştir. Kontrol olarak kullanılan standart antibiyotikler (Vankomisin, Flukonazol) ile karşılaştırıldığında, liken özütünün bazı mikroorganizmalarda yüksek derecede biyolojik aktivite gösterdiği gözlenmiştir. Bu bulgular, Antarktika likenlerinden izole edilen doğal ürünlerin, yeni antibiyotik geliştirmeçalışmaları ve biyoteknolojik uygulamalar için umut vadeden biyoaktif bileşikler içerebileceğini göstermektedir. Ayrıca, liken metabolitlerinin ekonomik ve hızlı izolasyonu ile ilgili daha fazla araştırmaya ihtiyaç duyulmaktadır. Sonuçlar, Antarktika likenlerinin ilaç keşfinde değerli bir kaynak olabileceğini göstermektedir.</p
Modal Parameter Identification in Asphalt Concrete using Non-Destructive Vibrational and Vibroacoustic Experimental Modal Analysis Techniques
No full textYükseköğretimde eğitim programlarının sürdürülebilirliğiiçin program değerlendirmeEisner’ın Eğitsel Uzmanlık ve Alkin’in UCLA Modellerine yöneliktasarım önerileri
No full textEvaluation of the Effects of Acute Cisplatin Administration on Testicular and Ovarian Tissue in Rats Sıçanlarda Akut Sisplatin Uygulamasının Testis ve Yumurtalık Dokusundaki Etkilerinin Değerlendirilmesi
No full textAim: Cisplatin, one of the effective chemotherapeutics in cancer treatment, has the potential to affect the testis and ovary, leading to permanent or temporary infertility. This study aimed to determine the acute effects of cisplatin on testis and ovary histology and to investigate whether it induces any changes in immunohistochemical cyclooxygenase-2 (COX2), nuclear factor kappa B p65 (NFκB-p65), and heat-shock protein 70 (HSP70) levels. Material and Methods: The study was planned as four groups: male control, male cisplatin, female control, and female cisplatin. The cisplatin group rats were adminestered with 7 mg/kg cisplatin, and all rats were sacrificed 24 hours later. Hematoxylin-eosin and Masson’s trichrome stains were applied to testis and ovary tissues to examine their histopathological structure, and an immunohistochemistry staining protocol was applied to determine immunostaining intensity of COX2, NFκB-p65, and HSP70. Results: In the cisplatin group, a decrease in seminiferous tubule epithelium, an elevation in fibrotic response in the interstitial area, and a notable reduction in Johnson testicular biopsy score (p<0.001) were seen in the testis. In the ovary, atretic follicles (p=0.006) and luteal structures within the cortex, as well as vascular congestion (p=0.001), edema (p=0.001), and fibrotic areas within the medulla, were evident. These alterations resulted in a statistically significant increase in ovarian histoscores, except for leukocyte infiltration (p=0.322). In both tissues, cisplatin significantly increased the immunostaining intensity of COX2, NFκB-p65, and HSP70 compared to the control group. Conclusion: Acute cisplatin administration can induce tissue damage and pro-inflammatory response in the testis and ovary
Influence of Silica Fume on the Mechanical and Microstructural Properties and Life Cycle Assessment of Fly Ash-Based Geopolymer Mortar
No full textThis study aims to investigate the influence of substituting silica fume with fly ash in fly ash-based geopolymer mortars. The replacement ratios of silica fume were 2%, 4%, 6%, 8%, and 10% on a mass basis with fly ash. The NaOH was used as an alkali activator. The produced geopolymer mortars were heat cured at a temperature of 75 degrees C for various durations: 24 and 48 h. The workability, unit weight, flexural strength, and compressive strength were conducted on geopolymer mortars. Selected mortars underwent abrasion and elevated temperature tests (300 degrees C, 600 degrees C, and 900 degrees C) based on their mechanical and compressive strength performance. Additionally, thermogravimetric analysis (TGA) and field emission scanning electron microscope (FESEM) imaging for microstructure and life cycle assessment (LCA) analysis for environmental performance were conducted. According to test results, optimum replacement ratio of silica fume (%2-4) with fly ash showed better performance than that reference geopolymer mortar in terms of strength properties and environmental impact categories of global warming potential, terrestrial ecotoxicity and abiotic depletion. These results indicate that silica fume replacement within this range enhances geopolymerization while maintaining structural integrity
Astaxanthin promotes apoptosis by suppressing growth signaling pathways in HT-29 colorectal cancer cells
No full textColorectal cancer (CRC) is the third most frequently diagnosed malignancy globally and ranks second in cancer-related mortality. Despite advancements in therapeutic approaches, the need for novel, effective and less toxic treatment strategies remains critical. Astaxanthin (ATX), a naturally occurring xanthophyll carotenoid, has attracted attention due to its strong antioxidant, anti-inflammatory and anti-cancer properties. This study aimed to evaluate the antiproliferative and pro-apoptotic effects of ATX on CRC through its influence on key molecular pathways, involved in tumorigenesis. The human colorectal adenocarcinoma cell line HT-29 was treated with varying concentrations of ATX (10 µM and 20 µM) for 24 h. Cell viability was assessed using the XTT assay. The expression levels of HER2, EGFR, ERK1, ERK2 and mTOR were quantified via enzyme-linked immunosorbent assay (ELISA). Immunofluorescence staining was used to evaluate the expression of EGFR and caspase-3 proteins. ATX exhibited significant antiproliferative and pro-apoptotic effects on HT-29 cells, with an IC50 value of 10.98 µM at 24 h. Treatment with ATX (10.98 µM) led to a marked increase in caspase-3 expression and a significant reduction in EGFR levels. Additionally, HER2, ERK1 and ERK2 levels were significantly downregulated, while mTOR expression remained unaffected. Flow cytometry analysis revealed a significant increase in apoptotic cell populations following ATX treatment, compared to the control group. ATX exerts notable antiproliferative and pro-apoptotic effects on CRC cells, potentially through modulation of the EGFR/HER2/ERK signaling pathway. These findings suggest that ATX may serve as a promising candidate for further investigation as an adjunctive or standalone therapeutic agent in the treatment of CRC
