Repositorio Institucional INIA (Inst. Nacional de Innovacion Agraria)
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    Carbon stocks in coffee farms and secondary forest systems in the Peruvian Amazon rainforest

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    Secondary forests and coffee cultivation systems with shade trees might have great potential for carbon sequestration as a means of climate change adaptation and mitigation. This study aimed to measure carbon stocks in coffee plantations under different managements and secondary forest systems in the Peruvian Amazon rainforest (San Martín Region). The carbon stock in secondary forest trees was estimated using allometric equations, while carbon stocks in soil, herbaceous biomass, and leaf litter were determined through sampling and laboratory analysis. The biomass carbon stock in secondary forests was 132.2 t/ha, while in coffee plantations with Inga sp. shade trees it was 118.2 t/ha. Carbon stocks were 76.5 t/ha in coffee with polyculture farming, and the lowest amount of carbon was found in coffee without shade trees (31.1 t/ha). The carbon sequestered by coffee plants in all agroforestry systems examined had an average of 2.65 t/ha, corresponding to 4.63 % of the total carbon sequestered, being the highest stored in the coffee system with Inga sp. shade trees. A higher content of glomalin-related soil proteins (GRSP) was found in coffee without shade trees, with 18.5 mg/g. This is evidence that Inga sp. is the most compatible model of shade system for coffee farms. We recommend the conservation of secondary forests due to the greater biomass and carbon storage, and establishing coffee plantations with Inga sp. shade trees for its integral benefits, such as climate change mitigation

    Taller: Implementación de una unidad de gestión de I+D+i, basado en la norma UNE 166002 y NTP ISO 56002

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    Presentación del Taller: "Implementación de una unidad de gestión de I+D+i, basado en la norma UNE 166002 y NTP ISO 56002" en el marco del Primer Encuentro Nacional de Gestión Tecnológica “VinculaTech 2023” realizado los días 29 y 30 en Lima, Perú

    Elaboración de compost a partir de residuos de la cosecha de cacao

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    La presente publicación proporciona los conocimientos básicos para la elaboración de compost a partir de residuos de la cosecha de cacao

    Assessment of the genetic diversity and population structure of the peruvian andean legume, tarwi (Lupinus mutabilis), with high quality SNPs

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    Lupinus mutabilis Sweet (Fabaceae), “tarwi” or “chocho”, is an important grain legume in the Andean region. In Peru, studies on tarwi have mainly focused on morphological features; however, they have not been molecularly characterized. Currently, it is possible to explore the genetic parameters of plants with reliable and modern methods such as genotyping by sequencing (GBS). Here, for the first time, we used single nucleotide polymorphism (SNP) markers to infer the genetic diversity and population structure of 89 accessions of tarwi from nine Andean regions of Peru. A total of 5922 SNPs distributed along all chromosomes of tarwi were identified. STRUCTURE analysis revealed that this crop is grouped into two clusters. A dendrogram was generated using the UPGMA clustering algorithm and, like the principal coordinate analysis (PCoA), it showed two groups that correspond to the geographic origin of the tarwi samples. AMOVA showed a reduced variation between clusters (7.59%) and indicated that variability within populations is 92.41%. Population divergence (Fst) between clusters 1 and 2 revealed low genetic difference (0.019). We also detected a negative Fis for both populations, demonstrating that, like other Lupinus species, tarwi also depends on cross-pollination. SNP markers were powerful and effective for the genotyping process in this germplasm. We hope that this information is the beginning of the path towards a modern genetic improvement and conservation strategies of this important Andean legume

    Genetic diversity and population structure of a Peruvian cattle herd using SNP data

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    New-generation sequencing technologies, among them SNP chips for massive genotyping, are useful for the effective management of genetic resources. To date, molecular studies in Peruvian cattle are still scarce. For the first time, the genetic diversity and population structure of a reproductive nucleus cattle herd of four commercial breeds from a Peruvian institution were determined. This nucleus comprises Brahman (N = 9), Braunvieh (N = 9), Gyr (N = 5), and Simmental (N = 15) breeds. Additionally, samples from a locally adapted creole cattle, the Arequipa Fighting Bull (AFB, N = 9), were incorporated. Female individuals were genotyped with the GGPBovine100K and ma les with the BovineHD. Quality control, and the proportion of polymorphic SNPs, minor allele frequency, expected heterozygosity, observed heterozygosity, and inbreeding coefficient were estimated for the five breeds. Admixture, principal component analysis (PCA), and discriminant analysis of principal components (DAPC) were performed. Also, a dendrogram was constructed using the Neighbor-Joining clustering algorithm. The genetic diversity indices in all breeds showed a high proportion of polymorphic SNPs, varying from 51.42% in Gyr to 97.58% in AFB. Also, AFB showed the highest expected heterozygosity estimate (0.41 ± 0.01), while Brahman the lowest (0.33 ± 0.01). Besides, Braunvieh possessed the highest observed heterozygosity (0.43 ± 0.01), while Brahman the lowest (0.37 ± 0.02), indicating that Brahman was less diverse. According to the molecular variance analysis, 75.71% of the variance occurs within individuals, whereas 24.29% occurs among populations. The pairwise genetic differentiation estimates (FST) between breeds showed values that ranged from 0.08 (Braunvieh vs. AFB) to 0.37 (Brahman vs. Braunvieh). Similarly, pairwise Reynold’s distance ranged from 0.09 (Braunvieh vs. AFB) to 0.46 (Brahman vs. Braunvieh). The dendrogram, similar to the PCA, identified two groups, showing a clear separation between Bos indicus (Brahman and Gyr) and B. taurus breeds (Braunvieh, Simmental, and AFB). Simmental and Braunvieh grouped closely with the AFB cattle. Similar results were obtained for the population structure analysis with K = 2. The results from this study would contribute to the appropriate management, avoiding loss of genetic variability in these breeds and for future improvements in this nucleus. Additional work is needed to speed up the breeding process in the Peruvian cattle system

    Integrated metabolite analysis and health-relevant in vitro functionality of white, red, and orange maize (Zea mays L.) from the Peruvian Andean race Cabanita at different maturity stages

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    The high maize (Zea mays L.) diversity in Peru has been recognized worldwide, but the investigation focused on its integral health-relevant and bioactive characterization is limited. Therefore, this research aimed at studying the variability of the primary and the secondary (free and dietary fiber-bound phenolic, and carotenoid compounds) metabolites of three maize types (white, red, and orange) from the Peruvian Andean race Cabanita at different maturity stages (milk-S1, dough-S2, and mature-S3) using targeted and untargeted methods. In addition, their antioxidant potential, and α-amylase and α-glucosidase inhibitory activities relevant for hyperglycemia management were investigated using in vitro models. Results revealed a high effect of the maize type and the maturity stage. All maize types had hydroxybenzoic and hydroxycinnamic acids in their free phenolic fractions, whereas major bound phenolic compounds were ferulic acid, ferulic acid derivatives, and p-coumaric acid. Flavonoids such as luteolin derivatives and anthocyanins were specific in the orange and red maize, respectively. The orange and red groups showed higher phenolic ranges (free + bound) (223.9–274.4 mg/100 g DW, 193.4– 229.8 mg/100 g DW for the orange and red maize, respectively) than the white maize (162.2–225.0 mg/100 g DW). Xanthophylls (lutein, zeaxanthin, neoxanthin, and a lutein isomer) were detected in all maize types. However, the orange maize showed the highest total carotenoid contents (3.19–5.87 μg/g DW). Most phenolic and carotenoid compounds decreased with kernel maturity in all cases. In relation to the primary metabolites, all maize types had similar fatty acid contents (linoleic acid > oleic acid > palmitic acid > α-linolenic acid > stearic acid) which increased with kernel development. Simple sugars, alcohols, amino acids, free fatty acids, organic acids, amines, and phytosterols declined along with grain maturity and were overall more abundant in white maize at S1. The in vitro functionality was similar among Cabanita maize types, but it decreased with the grain development, and showed a high correlation with the hydrophilic free phenolic fraction. Current results suggest that the nutraceutical characteristics of orange and white Cabanita maize are better at S1 and S2 stages while the red maize would be more beneficial at S3

    The distribution of cadmium in soil and cacao beans in Peru

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    Peru is the eighth largest producer of cacao beans globally, but high cadmium contents are constraining access to international markets which have set upper thresholds for permitted concentrations in chocolate and derivatives. Preliminary data have suggested that high cadmium concentrations in cacao beans are restricted to specific regions in the country, but to date no reliable maps exist of expected cadmium concentrations in soils and cacao beans. Drawing on >2000 representative samples of cacao beans and soils we developed multiple national and regional random forest models to develop predictive maps of cadmium in soil and cacao beans across the area suitable for cacao cultivation. Our model projections show that elevated concentrations of cadmium in cacao soils and beans are largely restricted to the northern parts of the country in the departments of Tumbes, Piura, Amazonas and Loreto, as well as some very localized pockets in the central departments of Huánuco and San Martin. Unsurprisingly, soil cadmium was the by far most important predictor of bean cadmium. Aside from the south-eastern to north-western spatial trend of increasing cadmium values in soils and beans, the most important predictors of both variables in nation-wide models were geology, rainfall seasonality, soil pH and rainfall. At regional level, alluvial deposits and mining operations were also associated with higher cadmium levels in cacao beans. Based on our predictive map of cadmium in cacao beans we estimate that while at a national level <20 % of cacao farming households might be impacted by the cadmium regulations, in the most affected department of Piura this could be as high as 89 %

    Estudio de vigilancia tecnológica en el cultivo de papa

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    El presente documento muestra información tecnológica respecto al cultivo de papa. Se hizo la búsqueda y análisis de las patentes, investigaciones y proyectos nacionales, con el objetivo de conocer el escenario tecnológico mundial y nuestras habilidades para la adaptabilidad de las nuevas tecnologías. Este estudio está dirigido a los investigadores y gestores de la I+D+i de la institución y actores del Sistema Nacional de Innovación Agraria, como una herramienta toma de decisiones orientadas a desarrollar nuevas propuestas de investigación y desarrollo de tecnologías de alto impacto en el sector agropecuario del Perú.Contiene: 1. Resumen ejecutivo 2. Vigilancia tecnológica 3. Metodología 4. Análisis de patentes 5. Análisis de publicaciones científicas 6. Contexto tecnológico nacional 7. Conclusione

    Nueva variedad de papa INIA 333 - CHUGAYNA

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    La variedad de papa INIA 333 - CHUGAYNA se caracteriza por su buena estabilidad en rendimiento, precocidad (120-130 días), buen contenido de materia seca (21%) y buena calidad culinaria para consumo en estado fresco

    Manual de manejo agronómico de raíces y tubérculos tropicales

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    El Instituto Nacional de Innovación Agraria (INIA) a través del Manual “Manejo agronómico de raíces y tubérculos tropicales” presenta los resultados obtenidos en el manejo agronómico del Banco de Germoplasma de Raíces y Tubérculos Tropicales, durante más de 20 años de conservación e investigación en estos importantes cultivos. Esta información relevante permitirá incrementar su producción, conservación, mejoramiento genético y valoración.Contiene: 1. Introducción 2. Ashipa (Pachyrhizus tuberosus) 3. Sachapapa (Dioscorea trifida, D. alata) 4. Huitina (Xanthosoma sagittifolium) 5. Pituca (Colocasía esculenta) 6. Dale dale (Calathea allouia) 7. Papa aérea (Dioscorea bulbifera) 8. Referencias bibliográficas 9. Anexo

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    Repositorio Institucional INIA (Inst. Nacional de Innovacion Agraria)
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