E-Journal Center for Plant Conservation Botanic Gardens-LIPI (Indonesian Institute of Sciences / Lembaga Ilmu Pengetahuan Indonesia)
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Amorphophallus muelleri Blume dan Amorphophallus paeoniifolius (Dennt.) Nicolson dari Pulau Flores
No full textIsolation and Characterization of Nitrogen Fixing Endophytic Bacteria Associated with Sweet Sorghum (Sorghum bicolor)
Get PDFSweet sorghum (Sorghum bicolor) has high adaptability to the dryland and resistant to drought. Many endophytic bacteria live in association with their host and play crucial role in health and development of plant. The aims of this work were to isolate and characterize nitrogen-fixing endophytic bacteria associated with sorghum. Sorghum samples were collected from Cibinong Science Center, Cibinong, Bogor, Indonesia. Sorghum werecultivated in four different treatment (compost and inoculants (CI), inoculants (TI), compost without addition inoculants (CN), without compost and inoculants (TN). The samples were sterilized by surface sterilization method and isolation of endophytic bacteria was carried out using spread plate method on TSA medium 1/10. Characterization of endophytic bacteria to fix nitrogen were done using Jensen’s media and tested by specific primers to amplify nifH gene. A total of 89 isolates were successfully isolated from root and stem. Sixteen isolates were able to grow on Jensen’s media. Based on PCR amplification of nifH gene was observed the expected size of the nifH region
Screening and Physiological Characterization of Rice Rhizosphere Bacteria from Coastal Soil that Produce Indol Acetic Acid in Saline Condition
Get PDFClimate change due to global warming increase water sea levels. Increasing the frequency of rising sea levels to the mainland can cause salinity problems for agricultural land in coastal areas. It will affect on plant productivity. Planting varieties of crops tolerant to salinity is one of the solutions to overcome this problem. Application of indigenous Plant Growth Promoting Rhizobacteria (PGPR) are expected to further increase the tolerancy of crops to salinity. This study was conducted to select a number of rhizobacteria isolates from rice field which were tolerant to saline conditions and capable of producing phytohormonesauxin (IAA). A total of 48 rhizobacteria isolates from coastal rice field of Patimban (Ptb) and Eretan (Er), West Java have been analyzed. Among 48 isolates, 12 selected isolates produced IAA>30 ppm. Isolate Er B1.3 and Er B1.7a have ability to grow on minimal salt medium content with 15% NaCl. Both isolates is still capable of producing IAA with consecutive concentrations at 1,55 ppm and 3,1 ppm on minimal medium salt content with 10% NaCl. IaaM gene identification results prove that isolates Er B1.3 and Er B1.7a producing IAA through the IAM pathway. Based on 16S rRNA sequencing results known isolates Er B1.3 and Er B1.7a are closely related to Brevibacterium sp. and Aeromonas sp., respectively
REKAMAN BARU TUMBUHAN LUMUT SEJATI DI PULAU ENGGANO
Get PDFEnggano Island is a small island in northwest Bengkulu. The mosses diversity of Enggano Island has ever been reported, unfortunately it has never been identified to species. The inventory of Enggano’s mosses reported 49 species comprising 27 genera and 12 families. Aerobryopsis wallichii (Brid.) M. Fleisch is a dominant species of mosses in study site. Five families consisting of 17 genera and 49 species are new records to Enggano’s mosses. Two species namely are Fissidens robinsonii Broth. and Syrrhopodon semilimber (Mitt.) Besch are recorded as new record to Enggano Island and probably are also found in main island, Sumatera
Growth and Productivity Parameters of 4 Insertional-Mutant-rice Lines under Salinity Stress and their Insertion Patterns
Get PDFThis experiment was aimed to observe the agronomy traits of 4 insertion-mutant-transgenic-Nipponbare rice lines in salinity stress condition and to evaluate the insertion patterns. The lines, (60, 170, 480 and 654), had been selected for their salinity tolerance from previous salinity test experiments. This experiment was conducted from the germination stage until the harvest time by planting the experiment plants in poly-bags and put them in flow tanks. The rice plants were treated with electrical conductivity of 3 mS/cm by means of NaCl using tap water. Nipponbare rice was used as wild type control. Amplifications for hpt and bar genes were conducted using their specific primers and the agronomy traits were measured of the experiment plants from germination stage, vegetative stage (plant height, tiller number) and reproductive stage (heading, seed number, productive tiller number). The observation of agronomic traits of the 4 mutant rice lines showed that each rice mutant lines of 60, 170, 480 and 654 has their own superior agronomic traits. However, the 170 rice mutant line is dominating the agronomic traits from germination and vegetative stage when treated with salinity stress. It has the smallest reduction percentages of germination, plant height in germination stage and length of flag leaves. In the productive stage, the line with the best reduction percentage for average of seed number per tiller was line 60; while the mutant rice line with the best reduction percentages of tiller number and plant height was line 654. The result of the PCR amplifications of hpt and bar genes showed different insertion patterns of each mutant line
Planning and Inventory Control Analysis at Perum Bulog West Java Regional Division
Get PDFWith expanded population, rice consumption in Indonesia is also increasing. Hence rice supply must be managed by Perum Bulog as the handler. Perum Bulog responsibility includes raskin distribution, government rice reserve distribution for emergency and disaster, rice distribution to government agencies and rice distribution among Perum Bulog regional division. Particularly in Perum Bulog West Java Province as one of the largest rice producer in Indonesia. The problems foundwere how to organize and inventory all those rice provided in West Java Province so it can be distributed evenly and efficiently. Solution to these problems will be soughtusing Economic Order Quantity (EOQ) model and approached with Period Order Quantity (POQ).Following complete analysis, it is noted that the largest rice procurement in the last five years happened in 2012 with 585.869.610,53 kg rice produced, and the lowest is going on in 2014. This stock instability is affected by several factors such as crop failures, or unpredictable climate changes thus caused several rice redeployments from DKI Jakarta, Central Java, and South Sulawesi regional division. For 2016, the cumulative cost of supplies calculated was Rp. 4.659.583.712.750,62 with ordering period as much as 71 times and will save the cost of supplies by Rp. 22.118.461.655,86 than planned by Perum Bulog West Java regional division
Effect of Plant Growth Promoting Rhizobacteria and Molasses on Seed Germination and Seedling Growth of Sorghum bicolor L.Moench
Get PDFGermination is the first step of the activity of plant growth. It is important for the next step in the field. The aim of the study was to evaluate the effect of PGPR and molasseson both germination (in vitro and soil) of Sorghum bicolor. The PGPR inoculants used were Phosphate Solubilizing Bacteria (Bacillus sp.) and Nitrogen Fixing Bacteria (Azospirillum sp, Azotobacter sp). Sorghum seed were treated with PGPR (sole and mixed inoculation) and molasses either singly or in combination. The result showed that in vitro experiment, seed inoculation significantly enhanced root and shoot length, and index vigour of sorghum. Conversely, seed germination were negatively affected by molasses application. In pot experiment, the application of both PGPR and molasses increased root and shoot length, and also root and shoot dry weight. The highest root and shoot length, root and shoot dry weight was obtained with s eed treated in combination PGPR mixed inoculation and molasses
Rhizosphere Microbial Genetic Resources as PGPR Potential Isolated from Maize Inbred Populations Var.Bisma
Get PDFA number of rhizosphere bacteria affect plant growth through contributing to the host plant-source of endogenous phytohormones. This study aims to obtain potential microbial genetic resources which isolated from corn. A total of 24 potential microbial genetic resource collections were isolated from the rhizosphere population of inbred corn plant var. Bisma obtained from the Biogen-Plant GeneBank collection. The potential of microbial genetic resources observed include the ability to produce IAA, N2 fixation, Phosphate solubility, and their ability to stimulate corn seed germination. Rhizosphere bacteria were isolated using the soil extract media. Isolates that showed the ability to produce IAA, N2 fixation and high phosphate solubility were tested for corn seed germination. The results showed that there were 24 isolates obtained from corn rhizosphere which dominated by bacilliform-shaped gram-positive bacteria capable of IAA producing ranged from 4.83 to 125.84 ppm. Almost 16 rhizosphere bacterial isolates were capable of dissolving phosphate with the phosphate solubility index ranged from 2.1 to 4.6. The selected potential isolates of J11, J16 and J19 were able to stimulate the corn seed germination
