E-Journal Center for Plant Conservation Botanic Gardens-LIPI (Indonesian Institute of Sciences / Lembaga Ilmu Pengetahuan Indonesia)
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Ethanol Production Using Cellulolytic, Xylanolytic and Fermentative Yeast on Cassava Waste
The objective of study was to produce ethanol using cellulolytic yeast SporidiobolusruineniaeBS-070 and fermentative yeast Saccharomyces cerevisiaeJSAT13-2-Y249 on cassava waste. Cassava waste was selected as substrate for ethanol production since it contained high polysaccharide. The process for ethanol production was divided by two steps. First step was hydrolyses of polysaccharide into fermentable sugar which was performed by SporidiobolusruineniaeBS-070. The second step was fermentation by fermentative strain Saccharomyces cerevisiaeJSAT13-2-Y249. Very high fermentable sugar was achieved after 48 hours incubations, and maximum ethanol (4500 mg/L) was achieved after 96 hours incubation. The co-culture of SporidiobolusruineniaeBS-070 and fermentative yeast Saccharomyces cerevisiaeJSAT13-2-Y249 are quite promising to produce biofuel ethanol on cassava waste. This implies the fermentation of cassava waste will solve environmental problem and produce sustainable biofuel
STUDI ETNOBOTANI TUMBUHAN OBAT DI KAWASAN SEKITAR DANAU BUYAN-TAMBLINGAN, BALI
The forest areas around Buyan-Tamblingan Lake have a high biodiversity value. At the same time, the expansion of agricultural lands and settlement is continuing in the region. Therefore, the conservation of plants and documentation of local knowledge in the area around Buyan-Tamblingan Lake is necessary. This study aims to determine the diversity of plants used as medicine by the communities around Buyan-Tamblingan Lake and to find out what plant species are considered most important by these community groups. This study was conducted for five days on 9 – 14 March 2015. Ethnobotanical data were collected using a method of semi- structured interviews and group discussions. The purposive selection of respondents sought those considered to have the best knowledge of ethnobotany. A total of five respondents were interviewed. This study documented as many as 69 species of medicinal plants being used, belonging to 59 genera and 36 families, with the Zingiberaceae being the most widely used. The floristic region of all plant species documented included Malesiana (21.95%), India (18.90%), Indochina (16.46), and East Asia (9.15). Leaves were the part of plants most often used. Plant species that had the highest use value (UV = 1) were Acorus calamus L., Cocos nucifera L., Curcuma longa L., and Zingiber officinale Roscoe. As many as 37 different ailments were mentioned by communities around Buyan-Tamblingan Lake including rheumatic, heartburn, and headaches
Stastus Populasi Tumbuhan Langka Rafflesia bengkuluensis Susatya, Ariyanto & Mat-Salleh di Kabupaten Kaur, Bengkulu Selatan
Increasing Added Value of Vanilla through Technology Utilization of Registered Patent
Vanilla has become Indonesia's export commodities since 1969. Although it does not belong to Indonesia’s indigenous plants, vanilla can be grown well in some regions of the country. Potential use of vanilla in food, cosmetics, and pharmaceuticals industry, causes value of this commodity continuously maintained. Indonesian vanilla export in the form of dried vanilla bean reaches approximately 80% of the total exports of Indonesian vanilla. However, added value of vanilla will be higher if the vanilla exported as processed product rather than dried vanilla beans. Therefore, this study aims to explore vanilla processing technology from patent documents to obtain a vanilla processing technology that can be applied in Indonesia without violating the law. Patent document searching was done through the free websites of patent documents provider such as Patentscope, freepatensonline,googlepatent, and espacenet, and the through a paid searching program such as Matheo Patent. This study use descriptive method where the data resulted from searching process were grouped into raw vanilla beans processing technology, vanilla processed product manufacturing, and vanilla waste or spent optimization of utilization. The results show some technologies that can be applied in Indonesia, which are US 2011/0081448, US 005705205, and EP 2430925. Based on the patent document searching results, added value of vanilla can be achieved through vanilla processing technologies combination of incubation technique, vanilla extract production using enzyme, and spent vanilla utilization of sing enzymes and microorganism
Effect of fertilization application on population and diversity of actinomycetes from rhizosphere soils of Sorghum bicolor
The aim of the study was to observe the effect of gradient-N fertilization, compost, microbial inoculant and its combination on population, diversity of actinomycetes, and their antibacterial and enzymatic activities from rhisosphere soils of Sorghum bicolor. Sorghum were cultivated in different fertilizers treatments. Fertilizer treatments including gradient-N chemical fertilizer alone (0%, 50% or 100% NPK) (TN0, TN50, TN100), gradient-N chemical fertilizer + microbial inoculant (TI0, TI50, TI100), gradient-N chemical fertilizer + compost (CN0, CN50, CN100 ), gradient-N chemical fertilizer + compost + microbial inoculant (CI0, CI50, CI100). Soil samples were collected from each rhizospheres of sorghum that has been treating with fertilization application for 100 days. The samples were air dried at room temperature for 7 days. One gram of each air-dried soil sample was pretreated with 0.05% sodium dodecyl sulfate (SDS). Humic acid vitamin agar (HVA) was used as selective media for isolation of actinomycetes. The application of different fertilizer treatments significant effected of population actinomycetes. The highest population of actinomycetes was isolated from rhizosphere soil with the application of CI50. The lowest population of actinomycetes was isolated from rhizosphere soil with the application of CN100 and CN50. A total 126 isolates of actinomycetes were obtained from 12 samples of rhizosphere soil from various treatment based on morphological characteristics. Among the 126 isolates, 95 isolates had aerial mycelium, and only 24 isolates produced soluble pigment into medium. The diversity of actinomycetes in all fertilizer treatments with addition of 50% NPK (except CI50) was higher than that in the other fertilizer treatments. Among the 126 isolates, 45 isolates showed antibacterial activity against B. subtilis. 54 isolates showed activity against E. coli, 101 isolates showed to produce cellulase, and 44 isolates showed produce phosphatase