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ExoSense: A novel technology for selective purification of CD63+ exosomes from neural cells
Get PDFExosomes are extracellular, cell signaling microvesicles that contain unique genomic and proteomic signatures reflective of the host cell’s pathophysiological conditions. In recent years, the study of exosomes has increased tremendously because many have been recognized as molecular biomarkers with the potential to advance methods of disease diagnostics and therapeutics as well as contribute to physiological analyses of multiple organism types. With the promising potential that exosomes offer to the field of molecular biology, it is vital to establish an efficient and consistently reliable mechanism of exosome isolation from biological samples. Many isolation techniques currently available typically yield exosome samples with numerous contaminants, making them low in purity for exosome content. Our lab has developed a novel technology for solid-phase exosome purification directly from biological samples. This study utilizes SDS-PAGE and fluorescent imaging analysis to assess the specificity of the ExoSense microprobe-based exosome isolation technology. The proteomic profile generated from the SDS-PAGE shows fewer bands with a cleaner background for the microprobe-based sample compared to the traditional polymer precipitated exosome proteins, suggesting an exosome population higher in purity from the microprobes than what the polymer reagent provides. Fluorescent imaging resulted in distinct differences between the control and exosome-specific groups, indicating an exosome-specific population captured using the probes. Results from this study can be used collectively to validate the specificity of the novel ExoSense exosome capture technology. Future studies can be applied for optimizing the technology for commercial applications
Healthcare and Patient Care during COVID-19 Pandemic
No full textCheck out the seminar series on CISCO Webex to register and view the recordings
Methacrylated Collagen and HNT Composite Hydrogels for Application in Bone Tissue Regeneration
Get PDFFractures and segmental bone defects are the primary cause of patient morbidity and brings a substantial economic burden to the healthcare system. Bone grafts used for bone injuries, tumors, and other pathologies related to poor fracture healing in the United States cost considerable money each year. The total cost of treating bone defects is about 5 billion US dollars. Autologous bone transplantation is the ideal method for the treatment of bone defects. However, their clinical results are variable and increase postoperative morbidity (especially at the donor site) and surgical costs. To circumvent these limitations, tissue engineering and cell-based therapies have been proposed as alternative methods to induce and promote bone repair.
In this study, we have developed a composite photo-crosslinked hydrogel with favorable mechanical properties and tunable bioactive properties. Furthermore, this composite hydrogel system, when combined with 3D printed scaffolds, can be modified to meet various applications for bone tissue regeneration applications. In this study, we identified the optimal combination between different concentrations of halloysite nanotubes (HNTs), strontium coated HNTs (SrHNTs), bone morphogenetic protein 2 (BMP-2), collagen methacrylated (COMA), and cross-linking time to develop a suitable scaffold. The scaffold is biocompatible and biodegradable, but also antibacterial and should promote faster healing.
The results suggest that gentamicin+SrHNTs+BMP-2 COMA hydrogel combined with a polycaprolactone (PCL) scaffold provides an optimal scaffold that can match the mechanical properties of bone. The next stage is to explore the scaffolds’ application in biomedical engineering. To do this, animal testing will need to be performed. If the scaffold works in the animal model it will provide a meaningful treatment plan for bone tissue repair and regeneration
Halloysite Modified 3D Printer Filament for Medical and Industrial Uses
Get PDFThermoplastics can be used as a replacement or alternative for a variety of medical and commercial products. The addition of halloysite nanotubes further enhance the strength and functionality of the composite. The aim of this project was to evaluate the capability polylactic acid (PLA) to be enhance with halloysite nanotubes and other additives. We created a nanocomposite that offered similar stabilization to titanium surgical plates but with the added benefit of bone generation through recruitment and differentiation of mesenchymal stem cells. Varying amounts of PLA and polycaprolactone were combined with growth factor doped halloysite nanotubes or silicon nitrate then extruded into 3D printer filament. Once 3D printed from the custom filament, the nanocomposite was subjected to mechanical and cell culture testing. Human mesenchymal stem cells were exposed to the 3D printed nanocomposites and monitored for osteogenic differentiation.Additionally, metalized halloysite nanotubes (mHNTs) were added to PLA to make an antibacterial 3D printer filament. Testing of both gentamicin loaded and unloaded mHNTs embedded PLA nanocomposites was conducted on E. coli and S. aureus. The PLA-mHNT filament was used to make a 3D printed antibacterial mask. Blow-spun fibers made of PLA and mHNTs were used as the filter component, which was added to the antibacterial mask to make an antibacterial respirator
But I Could be Marble
Get PDFGrace Miholic is a sophomore and a double major in French and English with a concentration in literature. She’s been writing poetry since the seventh grade, and her dream is to one day publish a book of all her poems. She’s looking forward to writing more and becoming a published author
That One Dime
Get PDFAnthony Franklin began his creative writing career in eighth grade after reading The Tell-Tale Heart by Edgar Allen Poe. Self expression through ink on paper is enthralling to him. He hopes to master this medium of expression, becoming the best writer he can be, throughout college and beyond
The View
Get PDFKatelyn Swanson is a sophomore at Louisiana Tech University studying English with a concentration in creative writing. Katelyn has goals of becoming a published author. She fell in love with writing in high school, and the rest has been history. She loves experimenting with different styles of writing and in different types of art as well. When she is not creating, you can find her playing video games in her room or cheering on the Bulldogs on the football field with the LA Tech color guard
David
Get PDFHunter Jones is majoring in English with a concentration in creative writing. In his free time, he enjoys practicing photography and videography
Silhouette
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Camera Shy
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