Osaka Dental University Academic Repository / 大阪歯科大学学術リポジトリ
Not a member yet
211 research outputs found
Sort by
血管老化診断キット開発におけるヒト唾液中参照遺伝子の探索
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Identifying reliable biomarkers in saliva can be a promising approach to developing a rapid diagnostic kit for detecting vascular aging. This study investigated the most suitable reference gene for PCR in saliva that is not affected by vascular aging variables. Whole saliva samples were collected to assess the expression of reference genes: actin beta (ACTB), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The most abundantly expressed gene was 18S rRNA, and the least expressed gene was GAPDH. Four genes were ranked according to their relative stability, as determined by mathematical algorithms, indicating that ACTB and 18S rRNA were stably expressed as reference genes. 18S rRNA was identified as the most promising reference gene for detecting systemic diseases using saliva from patients with vascular aging in these limited experimental conditions.doctoral thesi
ヒト歯肉由来線維芽細胞のTIMP-1産生に対するPDGF-bbの影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Matrix metalloproteinases degrade the extracellular matrix during remodeling, and their activity is regulated by tissue metalloproteinase inhibitors (TIMPs). Platelet-derived growth factor (PDGF) is produced in periodontal tissue and is an important regulator of wound healing and tissue repair. ERK1/2, members of the MAPK family, are intracellular signaling substance kinases involved in TIMP-1 production. To elucidate the mechanisms underlying connective tissue remodeling, the present study investigated the relationship between PDGF-bb-stimulated TIMP-1 production and ERK1/2 phosphorylation in human gingival fibroblasts (HGF). TIMP-1 production increased in a PDGF-bb concentration-dependent manner. PDGF-bb-induced increases in TIMP-1 were further enhanced by the p38α/β inhibitor SB239063 and p38α-siRNA. The PDGF-bb stimulation also induced the phosphorylation of ERK1/2. In contrast, the treatment with the p38-MAPK inhibitor SB203580 suppressed the phosphorylation of ERK1/2. These results suggest that PDGF-bb induced the production of TIMP-1 through the ERK1/2-activated signal transduction pathway in HGF. In addition, signaling cross-talk between p38α and ERK1/2 was involved in ERK1/2 activation, suggesting that ERK1/2 activation is coordinately regulated by p38α.doctoral thesi
歯牙欠損に対する補綴治療が効用値に与える影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）The purpose of this study was to determine the presence or absence of systematic errors in the utility scores of the deficient states and the respective utility scores of each deficient state with treatment intervention, for all Japanese, males, and females separately. Seventeen oral conditions (five missing tooth conditions and each condition with treatment interventions) were assumed. Utility scores calculated by the time trade-off method in a previous study were used. It was found that the utility score basically decreased as the number of missing teeth increased in the Japanese population as a whole and for both meles and females. The utility score increased with prosthetic treatment. Significant differences were found between full denture (FD) - implant over denture (IOD) and FD - implant-supported fixed dental prostheses (ISFDP) for males and between FD - IOD for females in the upper and lower edentulous. The results revealed significant differences in utility scores by deficiency status and the value of treatment intervention in the Japanese population as a whole and by gender. The results also suggest that prosthetic treatment is factors that improves one's utility score, and that there are differences in consultation behavior between meles and females depending on oral status.doctoral thesi
メインテナンス時の機械的歯面清掃がインプラントのアバットメント表面に与える影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）In this study, we assessed the influence of professional mechanical tooth cleaning (PMTC) on the surface roughness of implant abutments. This study aimed to determine the optimal PMTC conditions for effectively cleaning the abutment surface without causing damage. To achieve this, we investigated the effects of load, rotation speed, and polishing time on surface texture during PMTC. We utilized a titanium disk measuring 5 mm in diameter and 1.5 mm in thickness. Using three types of pastes with different abrasive particle sizes, PMTC was performed at 1500 rpm, 250 gf, and 15 s, with different combinations of load, rotation speed, and polishing time. The roughness of the disk surface before and after PMTC treatment was compared using scanning electron and confocal laser scanning microscopes. In PMTC, using a paste with large abrasive particles, both load and polishing time influenced surface quality. Furthermore, even with light pressure and short duration, surface roughness increased, highlighting the suitability of using a paste with small abrasive particles for PMTC.doctoral thesi
Er:YAGパルスレーザーデポジションを使用してハイドロキシアパタイトをコーティングした歯科用インプラントの生体適合性
Get PDF大阪歯科大学Osaka Dental University博士（歯学）We aimed to improve the biocompatibility and osteoinductive potential of Ti implants using a simulated intraoral hydroxyapatite (HAp) coating. We devised a novel surface treatment for aggressive induction of osteoblast adhesion and bone regeneration on the implant surface. A thin α-tricalcium phosphate (α-TCP) film was deposited on the implant surface using a pulsed Er:YAG laser. The coating was converted to HAp through artificial saliva immersion, which was confirmed using scanning electron microscopy (SEM) and X-ray diffraction (XRD). SEM showed needle-like hydroxyapatite crystals on the Ti disks and sandblasted implant surfaces after immersion in artificial saliva for 96 h. Microcomputed tomography and histological evaluation 4 and 8 weeks after implantation into beagle dog mandibles showed that the HAp-coated implant was biocompatible and exhibited superior osteoinduction compared to that of sandblasted implants. Coating the implant surface with HAp using an Er:YAG laser has potential as a new method of the implant-surface debridement.doctoral thesi
ラット骨髄細胞培養における石灰化結節形成に対するビタミン添加の効果
Get PDF大阪歯科大学Osaka Dental University博士（歯学）The present study attempted to obtain factors that induce the calcified nodule formation in a mesenchymal stem cell (MSC) culture through the aggregation of micro- or nanoparticles, such as calcium carbonate. There are few suitable cell sources in the oral cavity other than dental pulp tissue, from which only a small number of MSCs may be obtained. However, a large number of MSCs are needed for tooth regeneration. Therefore, factors that promote MSC proliferation and differentiation are required. Vitamin B1 (VB1), vitamin B3 (VB3) and vitamin B5 (VB5), which are present in physiologically active substances, were selected and investigated their effects to bone formation in vitro. VB1 and VB3 significantly increased the ability of dexamethasone (Dex) to form calcified nodule aggregates in vitro, whereas VB5 exerted the opposite effects. Therefore, it was confirmed from the results of this in vitro study that VB1 and VB3 may affect to Dex to promote MSCs in rat bone marrow cells for proliferation and differentiation into hard tissue-forming cells and the formation of calcified nodule aggregates.doctoral thesi
口腔癌細胞に対する近赤外光光線力学療法の有効性
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Oral cancer is a common malignant cancer, which is difficult to treat with traditional methods. Photodynamic therapy is a new alternative that is gaining widespread attention. We investigated the therapeutic effectiveness against oral cancer cells, and the mechanism of action of near-infrared photodynamic therapy (NIR-PDT) combined with the photosensitizer Chlorin e6 (Ce6) and up-conversion nanoparticles NaYF4:Yb/Er (UCNPs). After using the MTT (3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide)assay to determine a suitable concentration combination, human squamous cell carcinoma cells (HSC-3s) were treated with NIR-PDT combined with Ce6 and UCNPs. We examined cell proliferation and death by MTT assay, cell membrane permeability by live or dead cell staining, apoptosis by the FITC-Annexin V/PI assay, intracellular singlet oxygen and reactive oxygen species (ROS). NIR-PDT with 0.5ng/µl Ce6 and 0.1ng/µl UCNPs inhibited the cell proliferation of HSC-3s (p < 0.05). NIR-PDT led to an increase in intracellular singlet oxygen and ROS (p < 0.05), and changes in mitochondrial membrane potential and apoptosis occurred (p < 0.01). At the same time, the permeability of the cell membrane increased over time (p < 0.05). These results suggest that this treatment can inhibit the proliferation of oral cancer cells and induce apoptosis.doctoral thesi
IL-17はRAW264.7細胞のRANKL誘導性破骨細胞分化をダウンレギュレートする
Get PDF大阪歯科大学Osaka Dental University博士（歯学）In orthodontic treatment, tooth movement occurs based on bone remodeling in response to orthodontic forces. Interleukin-17A (IL-17) is a proinflammatory cytokine that is primarily secreted by Th17 cells. In the present study, we investigated the mechanisms by which trans-forming growth factor b-activated kinase 1 (TAK1) inhibits osteoclast differentiation and the role of IL-17 in the intracellular signaling pathways IkB kinase a (IKKa) and nuclear fac-tor-kB (NF-kB) p65, which are downstream of mitogen-activated protein kinases (MAPKs) (p38 and c-Jun N-terminal kinases (JNK)). We confirmed that IL-17 dose-dependently sup-pressed tartrate-resistant acid phosphatase (TRAP) activity in RAW264.7 cells in the presence of receptor for activation of NF-kB ligand (RANKL). We also found that a treatment with takinib, a potent and selective TAK1 inhibitor, significantly inhibited RANKL-stimulated TRAP activity in RAW264.7 cells. Furthermore, we demonstrated that IL-17 reduced the phosphorylation of TAK1, IKKa, and NF-kB p65. Collectively, these results suggest that IL-17 suppressed RANKL-induced osteoclast differentiation by inhibiting the canonical NF-kB pathway via TAK1. Therefore, IL-17 may be one of the factors suppressing RANKL-induced osteoclast differentiation. In the future, we would like to contribute to the development of reagents that locally control osteoclast differentiation by combining with nanocapsules that selectively deliver IL-17 to osteoclast progenitor cells.doctoral thesi
脱水熱処理条件がエピガロカテキンガレート結合ゼラチン材料に及ぼす影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Gelatin-based materials are promising biomaterials for applications in medicine and dentistry. Dehydrothermal treated (vacuum heated) epigallocatechin-conjugated gelatin sponges with or without beta-tricalcium phosphate (β-TCP) granules show greater bone-forming ability than those without this treatment. However, there is a paucity of information associated with the effect of vacuum heating conditions for these materials. In this study, we verified the changes in temperature and time during vacuum heating for the surface topography and viscoelasticity of epigallocatechin-conjugated gelatin sponges with or without β-TCP granules. In scanning electron microscopic observation, there were negligible changes in microstructure or surface topography of epigallocatechin-conjugated gelatin sponges up to 200°C for 4 h or 150°C for 24 h. Meanwhile, in the dynamic viscoelasticity test, the values of the storage and loss moduli remarkably increased beyond 100°C after 4 h heating or extending the heating time up to 16 h at 150°C. This increase was presumably associated with cross-linking in the epigallocatechin-conjugated gelatin. These results offer insights into the use of dehydrothermal treatment with vacuum heating for gelatin-based biomaterials with polyphenols.doctoral thesi
PEEKと接着性レジンセメントの接着強さに対して3級アミン浸漬処理の浸漬温度と浸漬時間が与える影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）This study aimed to investigate the effects of amine immersion temperature and duration on the bond strength between PEEK and adhesive resin cements. Blocks of 10 mm×5 mm were fabricated using PEEK. A Stainless steel piece 5 mm in diameter was aluminablasted and bonded to the PEEK blocks with resin cement and immersed in water for 24h to test shear bonding. Shear bonding tests showed significant differences in immersion temperatures at 40℃ and 60℃ compared with 20℃; however, no significant difference
was observed at 40℃ and 60℃. In terms of immersion time, significant differences were observed for 30, 60, and 120 min compared to 30 sec and for 120 min compared to 30 min; however, no significant difference was observed for 30 and 120 min compared to 60 min. To improve the bond strength between PEEK and adhesive resin cement, we recommended immersion temperature and duration at ≥40°C and ≥60 min, respectively.doctoral thesi