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南米原産の生薬（C. ruber）抽出液はin vitroで糖化反応中間体による細胞毒性を緩和する
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Glycation is the non-enzymatic covalent attachment of a sugar to a protein, lipid, or nucleic acid molecule and is involved in many diseases. During disease onset and progression, it is thought that glycation end products provoke cytotoxicity. Although artificial and natural anti-glycation substances are regarded as preventive agents for glycation-induced toxicity, their properties and mechanism of action are not fully elucidated. In the current study, we focused on Connarus ruber extract, an herbal medicine that is considered effective against chronic diseases, such as diabetes and lipid disorders, and that has anti-glycation activity in vivo. We investigated whether C. ruber extract can prevent glycation-induced cytotoxicity against dental pulp stem cells (DPSCs), which are dental tissue-derived mesenchymal stem cells. C. ruber extract showed anti-glycation activity in vitro as well as in vivo. C. ruber extract also protected DPSCs from glycation intermediate-mediated cytotoxicity. These results indicate that C. ruber extract is a potent herbal medicine against diseases involving glycation.doctoral thesi
口腔外スキャンボディと歯列を含む口腔周囲スキャンを使用した歯列顔面モデルの精確さ
Get PDF大阪歯科大学Osaka Dental University博士（歯学）We investigated the accuracy of dentofacial models used in digital dental treatment,which are referred to as virtual patients. In Experiment 1, we investigated the integration method for the dentition and face of the reference virtual patient (RVP) used in Experiment 2, and decided to use the labial surface of the maxillary anterior teeth. In Experiment 2, we compared the accuracy of the dentofacial models integrated using three different methods. An RVP was created by registering the maxillary dentition model and facial model using the labial surface of the maxillary anterior teeth. The maxillary dentition model and facial model were integrated using the extraoral scan body as the integration interface to create a study virtual patient (SVP), Face Hunter study virtual patient (FH S),and Heges study virtual patient (HE S) using the dental face scanner (Face Hunter, FH) and a 3D scanning application (Heges, HE). In HE, an SVP was also created using a perioral scan including dentition as the integration interface (HE P). Registration was performed for the RVP and the three types of SVP. As indicators of the accuracy of the dentofacial model, we measured the deviation distance of the center of gravity of the maxillary dentition model, angular deviation of the frontal cant of the maxillary central incisor edge line, and angular deviation of the inclination of the maxillary dentition reference plane. We found that FH S showed clinically acceptable accuracy.doctoral thesi
Expression of elastin, F-box and WD-40 domain-containing protein 2, fibrillin-1, and alpha-smooth muscle actin in utilized blood vessels for explant culture － A new 3D in vitro vascular model from bovine legs.
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高脂肪食を投与した高齢マウスの顎下腺・舌下腺におけるCmpk2の発現
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Identification of reliable molecular markers is crucial for the development of effective diagnostic and treatment strategies for salivary gland (SG) dysfunction associated with aging and lifestyle-related inflammatory diseases. Cytidine/uridine monophosphate kinase 2 (Cmpk2) has recently been reported to be involved in various inflammatory diseases. This study examined the Cmpk2 expression in the SGs of high-fat high-cholesterol (HFHC) diet-fed aged mice. Eight-week-old male C57BL/6NCrSlc mice were divided into normal (normal diet) and HFHC (HFHC diet) groups and fed ad libitum for 72 weeks. At 80 weeks of age, HFHC group elevated Cmpk2 expression in both submandibular glands (SMGs) and sublingual glands (SLGs) compared with normal group. SLG exhibited more broader Cmpk2 expression compared with the SMG. Hematoxylin-eosin staining revealed that SLGs in HFHC group showed widespread acinar atrophy compared with those in normal group. The change was negligible in the SMGs. These findings suggest that the broad Cmpk2 expression in SLGS may reflect widespread atrophy, possibly associated with low-grade inflammation under HFHC conditions. Our findings highlight Cmpk2 as a potential biomarker for the development of effective salivary diagnostic methods for SG dysfunction and lifestyle-related diseases.doctoral thesi
Analysis of the impact of periodontal disease management before the onset of type 2 diabetes mellitus on medical costs using administrative claims database.
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Effect of xanthan gum-based, guar gum-based, and starch-based thickeners on tongue pressure and pharyngeal residue.
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ヒト歯根膜細胞における高血糖の酸化ストレスと miR-146a発現の相互制御
Get PDF大阪歯科大学Osaka Dental University博士（歯学）The high-glucose conditions caused by diabetes mellitus (DM) exert several effects on cells, including inflammation. miR-146a, a kind of miRNA, is involved in inflammation and may be regulated mutually with reactive oxygen species (ROS), which are produced under high-glucose conditions. In the present study, we used human periodontal ligament cells (hPDLCs) to determine
the effects of the high-glucose conditions of miR-146a and their involvement in the regulation of oxidative stress and inflammatory cytokines using Western blotting, PCR, ELISA and other methods. When hPDLCs were subjected to high glucose (24 mM), cell proliferation was not affected; inflammatory cytokine expression, ROS induction, interleukin-1 receptor-associated kinase 1 (IRAK1) and TNFreceptor-associated factor 6 (TRAF6) expression increased, but miR-146a expression decreased. Inhibition of ROS induction with the antioxidant N-acetyl-L-cysteine restored miR-146a expression and decreased inflammatory cytokine expression compared to those under high-glucose conditions. In addition, overexpression of miR-146a significantly suppressed the expression of the inflammatory cytokines IRAK1 and TRAF6, regardless of the glucose condition. Our findings suggest that oxidative stress and miR-146a expression are mutually regulated in hPDLCs under high-glucose conditions.doctoral thesi
炭酸カルシウム系新規知覚過敏抑制材料がヒト乳歯歯髄由来線維芽細胞のIntegrin αvβ3発現に与える影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Dental desensitizing materials are often applied to wedge defects, potentially allowing the material to come into contact with pulp cells. In this study, we aimed to clarify cell proliferation, calcium dynamics, and expression of integrin αvβ3 by culturing cells in a medium containing a calcium carbonate-based dental hypersensitivity treatment agent.
In this experiment, we performed a WST assay and observed fluorescently la- beled antibodies using a confocal laser scanning microscope. The number of cells increased in the experimental group compared to the control group on day 1 of incu- bation. On days 4 and 7 of incubation, material-derived calcium was localized in the cytoplasm of the experimental group, overlapping with the Integrin αvβ3 expression sites. It was suggested that calcium derived from the material is taken up into the cytoplasm and promotes cell proliferation in the early stages of culture.doctoral thesi
Shikoninがヒト歯肉上皮細胞の創傷治癒や抗炎症作用に及ぼす影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Phytochemicals are propenties, non-nutritive bioactive substances that often have antioxidant and anti-inflammatory. Shikonin, a aphthoquinone compound produced by plants of the family Murasaki-aceae, is the main active ingredient in the herbal medicine shikonin. We investigated the anti-inflammatory effect of shikonin on periodontal tissue using human gingival epithelial cells (epi4). The cells were treated with shikonin at concentrations ranging from 0.001 to 10 μM. Optimal concentrations were determined using cell proliferation and migration as well as cytotoxicity assays. Shikonin significantly promoted epi4 proliferation and migration. A concentration of 0.01 μM was selected as optimal. An inflammatory state was induced using 1 ng/mL of human recombinant Interleukin-1 (IL-1) β. The anti-inflammatory effect of shikonin at the optimal concentration was examined based on the expression of inflammatory cytokines IL-6 and IL-8, which was determined using enzyme-linked immuno-sorbent assays (ELISA) and real-time polymerase chain reaction (PCR). Western blot analysis was performed to examine Nuclear Factor Kappa B (NF-κB) p65 phosphorylation at 15 min after initiating stimulation using the optimal concentration of shikonin. In conclusion, shikonin exerts proliferative, migratory, and anti-inflammatory effects on epi4 cells and promotes wound healing in human gingival epithelial cells, suggesting that it may benefit periodontal healing.doctoral thesi
家兎における馬由来コラーゲン海綿骨ブロックを用いたインレーおよびオンレーグラフトの組織学的評価
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Background: The conformation of the recipient site for an inlay graft presents an increased contact with the parent bone compared to an onlay graft. This might favor bone growth within the inlay compared to onlay grafts. Hence, the objective of this study was to compare the bone incorporation and remodeling processes of xenogeneic en bloc grafts placed using two bone grafting techniques, i.e., onlay vs. inlay. Methods: In this prospective, randomized, split-mouth study (test and control sides in the same animal), two bone grafting techniques were comparatively evaluated. The lateral aspect of the rabbit mandible was used as the recipient site, bilaterally. On one side of the mandible, the cortical bone was perforated with drills to allow a better bone formation from the bone wound and the marrow spaces. A xenogeneic bone block was fixed in the center of the prepared region, representing the onlay site. On the other side of the mandible, a 7 mm wide and 3 mm deep circumferential defect was prepared using trephines and drills. A xenogeneic bone block was fixed in the center of the defect, representing the inlay site. Two healing periods were applied in the study: 2 and 10 weeks, each represented by 10 rabbits (n = 10 for each period). Results: After 2 weeks of healing, the mean percentage of new bone was 10.4% and 23.3% at the onlay and inlay grafts, respectively (p = 0.022). After 10 weeks of healing, new bone increased to 13.2% at the onlay sites and 25.4% at the inlay sites (p = 0.080). In the 10-week period, the inlay grafts presented a homogeneous growth of new bone in all regions, while in the onlay grafts, low percentages of new bone were observed in the external regions. Conclusion: The percentage of new bone increased faster and was higher in the inlay grafts than in the onlay grafts. This outcome might be related to the self-contained conformation of the recipient site in the inlay group, which offered more sources for new bone formation compared to the one-wall conformation of the recipient sites in the onlay group. The osteoconductive properties of the biomaterial allowed the newly formed bone to reach the most peripheral regions in both groups. The osteoconductive properties of the biomaterial, together with the protection offered by the collagen membrane, allowed marginal closure of the defects by newly formed bone in the inlay group.doctoral thesi