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The Potential and Effectiveness of Snail Seromucoid and Chitosan as Bioimmunostimulators
Get PDFThe host's cellular immune response plays an important role in the process of eliminating microorganisms that cause infection. Substances that can stimulate an increase in the immune response are called immunostimulators. Snail seromucoid contains bioactive compounds such as glycans, peptides, glycopeptides and chondroitin sulfate. Chitosan as an antimicrobial agent can be used in the biomedical field because chitosan has a number of hydroxyl groups (OH) and amine groups (NH2). The research objective was to determine the potency and effectiveness of snail seromucoid and chitosan as bioimmunostimulators. The research method is based on laboratory experimental results with the research stages, namely the analysis of the effectiveness of seromucoid and chitosan on lymphocyte proliferation. The results of the one way ANOVA analysis showed a p value of 0.000 so that there was a significant effect between the treatment groups, namely the effect of giving chitosan 65 ug/ml; snail mucus 65 ug/mL and a combination of chitosan (65 ug/mL) and snail mucus (65 ug/mL) ratio of 1:1, can increase lymphocyte proliferation optimally. This shows that snail mucus, chitosan and their combination in a 1:1 ratio are effective as BRM (Biological Response Modifier). It is hoped that the contribution of the results of this study can be further developed in the bioformulation of snail seromucoid preparations and chitosan as therapeutic agents for infectious diseases, including Acute Respiratory Distress Syndrome (ARDS), Tuberculosis and other diseases.The host's cellular immune response plays an important role in the process of eliminating microorganisms that cause infection. Substances that can stimulate an increase in the immune response are called immunostimulators. Snail seromucoid contains bioactive compounds such as glycans, peptides, glycopeptides and chondroitin sulfate. Chitosan as an antimicrobial agent can be used in the biomedical field because chitosan has a number of hydroxyl groups (OH) and amine groups (NH2). The research objective was to determine the potency and effectiveness of snail seromucoid and chitosan as bioimmunostimulators. The research method is based on laboratory experimental results with the research stages, namely the analysis of the effectiveness of seromucoid and chitosan on lymphocyte proliferation. The results of the one way ANOVA analysis showed a p value of 0.000 so that there was a significant effect between the treatment groups, namely the effect of giving chitosan 65 ug/ml; snail mucus 65 ug/mL and a combination of chitosan (65 ug/mL) and snail mucus (65 ug/mL) ratio of 1:1, can increase lymphocyte proliferation optimally. This shows that snail mucus, chitosan and their combination in a 1:1 ratio are effective as BRM (Biological Response Modifier). It is hoped that the contribution of the results of this study can be further developed in the bioformulation of snail seromucoid preparations and chitosan as therapeutic agents for infectious diseases, including Acute Respiratory Distress Syndrome (ARDS), Tuberculosis and other diseases
Pengaruh Ekstrak Etanol Tangkai Begonia multangula Blume Terhadap Biofilm Bakteri Aggregatibacter actinomycetemcomitans
Get PDFAggressive periodontitis is a type of peridontitis with rapid disease progression and destruction. One of the causes of aggressive periodontitis is the presence of Aggregatibacter actinomycetemcomitans biofilm. Prevention of biofilm formation is one way to prevent aggressive periodontitis. The stalk of B. multangula Blume has antibacterial activity and has the potential to be used as an antibiofilm. This research aimed to determined antibiofilm activity of etanol extract of the stalk B. multangula Blume against A. actinomycetemcomitans..
This research used 5 concentration of extract (6,25%, 12,5%, 25%, 50% and 100%), Negative Control (Aquades sterile) and Positive Control (CHX 0,2%). Inhibition biofilm test using microtitter plate biofilm assay with crystal violet staining at anaerobic incubation for 24 hours and using microplate reader at 620 nm. One way ANOVA and post hoc LSD were used to analyze the differences in antibiofilm activity.
The result showed inhibition biofilm activity of A. actinomycetemcomitans by the extract increased as increasing of the concentration. The highest biofilm inhibition was at 100% (74,92%) of the extract concentration and Minimum Biofilm Inhibition Concentration 50 (MBIC50) against A. actinomycetemcomitans was found at 25% (54,42%) of the extract concentration. There were significant difference (p≤0,05) between treatment group of etanol extract (concentration 6,25%, 12,5%, 25%, 50%, and 100%) with negative control and there were no significant difference (p≥0,05) in the positive control with 50% and 100% of the extract concentration. This study concluded that there was an antibacterial activity etanol extract of the stalk B. multangula Blume against inhibition biofilm of A. actinomycetemcomitans.Periodontitis agresif merupakan salah satu jenis penyakit gigi dan mulut yang menyerang jaringan periodontal dengan perkembangan penyakit dan kerusakan yang sangat cepat. Pencegahan periodontitis agresif dapat dilakukan dengan mencegah terbentuknya biofilm bakteri gigi. Salah satu bakteri tersebut adalah Aggregatibacter actinomycetemcomitans. Tangkai Begonia multangula Blume diketahui memiliki aktivitas antibakteri, tetapi aktivitasnya terhadap biofilm A. actinomycetemcomitans belum diketahui. Tujuan penelitian ini untuk mengetahui aktivitas antibiofilm ekstrak etanol tangkai B. multangula Blume terhadap bakteri A. actinomycetemcomitans.
Penelitian ini terdiri dari 5 kelompok perlakuan ekstrak etanol tangkai B. multangula Blume (6,25%, 12,5%, 25%, 50% dan 100%), Kontrol Negatif (Akuades steril) dan Kontrol Positif (CHX 0,2%). Pengukuran biofilm dilakukan menggunakan microtitter plate biofilm assay pada panjang gelombang 620 nm dengan pewarna kristal violet. Uji statistik one way ANOVA dan post hoc LSD dilakukan untuk menganalisis perbedaan ketebalan biofilm tiap perlakuan.
Hasil menunjukkan bahwa ketebalan biofilm pada perlakuan ekstrak etanol tangkai B. multangula Blume semakin rendah seiring dengan peningkatan konsentrasi. Penghambatan biofilm tertinggi terdapat pada konsentrasi ekstrak 100% (74,92%) dengan Minimum Biofilm Inhibition Concentration 50 (MBIC50) terdapat pada konsentrasi ekstrak 25% (54,42%). Uji statistik menunjukkan adanya perbedaan signifikan (p≤0,05) pada pemberian ekstrak etanol tangkai B. multangula Blume konsentrasi 6,25%, 12,5%, 25%, 50%, dan 100% dengan kontrol negatif serta tidak ada perbedaan signifikan (p≥0,05) pada perlakuan konsentrasi 50% dan 100% jika dibandingkan dengan kontrol positif dengan Simpulan penelitian ini ekstrak etanol tangkai B. multangula Blume memiliki aktivitas antibiofilm terhadap bakteri A. actinomycetemcomitans
PRESTASI AKADEMIK SISWA BERKEBUTUHAN KHUSUS DI SEKOLAH DASAR INKLUSIF: PERAN KETERLIBATAN ORANG TUA DAN MEDIASI KETERAMPILAN SOSIAL
Get PDFStudent academic achievement is the result of the interaction between environmental factors and individual factors. This study uses parental involvement as environmental factors and social skills as individual factors that can affect the academic achievement of students with special needs in inclusive elementary schools. This study aims to examine the theoretical model of students' social skills as mediator on the effect of parental involvement on the academic achievement of students with special needs in inclusive elementary schools. Participants in this study were parents of students with special needs (N=365) who studied in inclusive public and private elementary schools in five areas of DKI Jakarta. The results showed that social skills mediate the effect of parental involvement on the academic achievement of students with special needs in inclusive primary schools.Prestasi akademik siswa merupakan hasil interaksi antara faktor lingkungan dan faktor individu. Penelitian ini menggunakan keterlibatan orang tua sebagai faktor lingkungan dan keterampilan sosial sebagai faktor individu yang dapat mempengaruhi prestasi akademik siswa berkebutuhan khusus di sekolah dasar inklusif. Penelitian ini bertujuan untuk menguji model teoritik pengaruh mediasi keterampilan sosial siswa pada pengaruh keterlibatan orang tua terhadap prestasi akademik siswa berkebutuhan khusus di sekolah dasar inklusif. Partisipan dalam penelitian ini adalah orangtua dari siswa berkebutuhan khusus (N=365) yang belajar di sekolah dasar negeri dan swasta inklusif di lima wilayah Kota DKI Jakarta. Hasil penelitian menunjukkan bahwa keterampilan sosial memediasi pengaruh keterlibatan orang tua terhadap prestasi akademik siswa berkebutuhan khusus di sekolah dasar inklusif
Sistem Monitoring Gudang Dengan Sensor Ultrasonik Berbasis Microcontroller Arduino Pada PT XYZ
Get PDFThe rapid advancement of technology provides convenience for humans. The presence of the internet of thinks (IoT), is able to solve problems related to warehouse security. This study aims to be able to control warehouse security in real time remotely by utilizing SMS notification messages on mobile phones. Applying the prototyping research method, this research on warehouse monitoring systems with ultrasonic sensors based on the Arduino microcontroller is able to produce system security by providing hazard warning messages to warehouse supervisors. So that incidents of theft can be detected in a fast time.Semakin pesatnya kemajuan teknologi memberikan kemudahan bagi manusia. Hadirnya internet of thinks (IoT), mampu menyelesaikan masalah yang terkait dengan keamanan gudang. Penelitian ini bertujuan untuk bisa mengontrol keamanan gudang secara real time dari jarak jauh dengan memanfaatkan pesan notifikasi sms pada telepon genggam. Menerapkan metode penelitian prototyping, penelitian sistem monitoring gudang dengan sensor ultrasonic berbasis microcontroller arduino ini mampu menghasilkan keamanan sistem dengan memberikan pesan peringatan bahaya kepada pengawas gudang. Sehingga kejadian pencurian dapat diketahui dalam waktu yang cepat
SELF-CONFIDENCE DAN SELF-DISCLOSURE PADA MURID BARU DI MASA PANDEMI COVID-19
Get PDFAbstract
Most individuals build peer relationships where there is someone who is fun to be with and can make individuals comfortable being themselves. In line with the development of individual relationships tend to require acceptance from the environment. This study aims to determine whether there is a relationship between self-confidence and self-disclosure in new students at SMK X Malang Regency. This study uses a quantitative approach with the population in this study is the new students of SMK X in 2020/2021 as many as 114 people. The data collection technique uses a Likert scale instrument consisting of a self-confidence scale consisting of 30 items and a self-disclosure scale consisting of 30 items that have been tested for reliability and validity. The data analysis technique used the Pearson product moment correlation technique with SPSS version 22 for windows. The results showed that there was a relationship between self-confidence and self-disclosure. The higher the self-confidence, the higher the self-disclosure and vice versa. Individuals can develop their social skills during online schooling to establish relationships or interactions through self-disclosure by increasing their self-confidence.Sebagian besar individu membangun hubungan sengan teman sebaya dimana ada seseorang yang menyenangkan untuk bersama dan dapat membuat individu nyaman menjadi diri sendiri. Sejalan dengan perkembangan hubungan individu cenderung membutuhkan penerimaan dari lingkungan. Penelitian ini bertujuan untuk untuk mengetahui apakah ada hubungan antara Self-confidence dengan Self-disclosure pada siswa siswi baru SMK X Kabupaten Malang. Penelitian ini menggunakan pendekatan kuantitatif dengan Populasi dalam penelitian ini adalah murid baru SMK X tahun 2020/2021 sebanyak 114 orang. Teknik pengumpulan data menggunakan instrumen skala likert yang terdiri dari skala Self-confidence terdiri dari 30 item dan skala Self-disclosure yang terdiri dari 30 item yang telah di uji reliabilitas dan validitasnya. Teknik analisis data menggunakan teknik korelasi pearson product moment dengan SPSS versi 22 for windows. Hasil penelitian menunjukkan bahwa ada hubungan antara Self-confidance dengan Self-disclosure. Semakin tinggi self confidence maka semakin tinggi juga Self-disclosure demikian pula sebaliknya. Individu dapat mengembangkan keterampilan sosial mereka selama sekolah daring untuk menjalin hubungan atau interaksi melalui self-diclosure dengan meningkatkan self-confidance ny
Pengaruh Konsentrasi dan Waktu Perendaman Ampas Tebu terhadap Kadar Akrilamida pada Minyak Goreng Bekas
No full textHydrolyzed cooking oil will form glycerol and free fatty acids, so that when heated glycerol will form acrolein, one of the compounds forming acrylamide. Bagasse contains cellulose which can bind acrylamide. The purpose of this study was to determine the effect of adding pulp and the effect of soaking time on acrylamide levels.
Analysis was performed using High-Performance Liquid Chromatography, column Agilent TC-C18 mobile phase 0.1% phosphoric acid and methanol in a ratio of 95:5; flow rate 1 mL/min; the sample volume injected was 20µL. Acrylamide in oil was identified at a retention time of 4,939 minutes, theoretical plate 39,029, HETP 0.00064, and capacity factor 2.265
This study meets the method validation parameters such as linearity with the results of y = 259.37x + 103.39 and a correlation coefficient of 0.9993, precision with % RSD < 2%, detection limit 0.46 ppm, quantification limit 1.396 ppm, accuracy with % recovery 94 .55 - 100.04. The results of FTIR, bagasse can bind the C=O and C-H groups of one of the acrylamide groups. The adsorption kinetics model used was the Ho model with linearity of 0.9752. Sugarcane bagasse concentration of 5% and soaking time of 24 hours affected the decrease in acrylamide levels in used cooking oil. Soaking used cooking oil samples with 5% bagasse for 24 hours can reduce acrylamide levels by 65%.Minyak goreng yang terhidrolisis akan membentuk gliserol dan asam lemak bebas, sehingga ketika dipanaskan gliserol akan membentuk akrolein salah satu senyawa pembentuk akrilamida. Ampas tebu mengandung selulosa yang dapat mengikat akrilamida. Tujuan penelitian ini untuk mengetahui pengaruh penambahan ampas dan pengaruh lama perendaman terhadap kadar akrilamida.
Analisis dilakukan menggunakan Kromatografi Cair Kinerja Tinggi dengan kolom Agilent TC-C18. Analisis dilakukan menggunakan fase gerak asam fosfat 0,1% dan methanol dengan perbandingan 95:5, laju alir 1 mL/menit, dan volume sampel yang diinjeksikan sebanyak 20µL. Kapasitas adsorpsi akrilamida oleh ampas tebu ditentukan dengan studi kinetika.
Akrilamida pada minyak teridentifikasi pada waktu retensi 4,939 menit, plat teoritis 39.029, HETP 0,00064 dan faktor kapasitas 2,265. Metode ini terbukti valid dengan linearitas y = 25,34x + 103,32, koefisien korelasi 0,9994, SBR < 2% batas deteksi 0,46 ppm, batas kuantifikasi 1,396 ppm, % recovery 94,94 - 100,06. Model kinetika adsorpsi mengikuti model kinetika Ho dengan kapasitas adsorpsi sebesar 47,39 mg/g. Konsentrasi ampas tebu 5% dan lama perendaman 24 jam berpengaruh terhadap penurunan kadar akrilamida pada minyak jelantah. Perendaman sampel minyak jelantah dengan ampas tebu 5% selama 24 jam dapat menurunkan kadar akrilamida sebesar 65%
Evaluasi Rasionalitas Penggunaan Antidiabetes Oral pada Pasien Diabetes Melitus Tipe 2 Rawat Inap di Rumah Sakit Nasional di Dili
Get PDFAmong several types of diabetes mellitus (DM), type-2 DM seemed to demonstrate the highest prevalence. Appropriate management in type-2 DM should follow rational use of antidiabetic agents to prevent any unfavorable impacts. In fact, due to the limited guidelines and medication resources, medication process for patients with type-2 DM has not been evaluated in Timor-Leste, especially at a national hospital in Dili. This was the first study which aimed to evaluate the use of oral antidiabetic agents (OAD) in hospitalized patients with type-2 DM at a national hospital, Dili Timor-Leste.This study was conducted retrospectively under cross-sectional design to describe the rational drug use evaluation based on indicators: right patient, right dose, right indication, right drug, and cautious to adverse events according to guideline from American Diabetes Association (2020). The included patients were diagnosed as type-2 DM, hospitalized during year 2020, and received OAD. Data were calculated (%) for each indicator of rational drug use.A total number of 83 hospitalized patients with type-2 DM at a national hospital in Dili had taken metformin (62.65%), gliclazide (12.15%), and combination of both metformin and gliclazide (22.89%), respectively. The evaluation results of OAD use according to ADA guideline comprised of right indication 100%, right patient 100%, right drug 72.28%, right dose 100%, and cautious to adverse events 93.7%. Despite favorable results in rational medication, enhancement for limitation of OAD use at a national hospital in Dili, Timor-Leste is compulsory.Di antara beberapa tipe penyakit diabetes melitus (DM), angka kejadian tertinggi terlihat pada DM tipe 2. Pengelolaan DM tipe 2 yang tepat sebaiknya mengikuti intervensi farmakologi antidiabetes secara rasional untuk menghindari dampak buruk. Dalam keterbatasan pedoman dan resource pengobatan, evaluasi rasionalitas pengobatan pasien DM tipe 2 belum pernah dilakukan secara luas di Timor-Leste, khususnya pada salah satu RS Nasional di Dili. Penelitian ini bertujuan untuk mengevaluasi penggunaan antidiabetes oral (ADO) pada pasien DM tipe 2 rawat inap di salah satu RS Nasional di Dili periode 2020.Penelitian dilakukan dengan desain cross-sectional retrospektif. Rasionalitas dinilai berdasarkan indikator tepat pasien, tepat dosis, tepat indikasi, tepat obat, dan waspada efek samping yang mengacu pada American Diabetes Association (ADA) tahun 2020. Pasien yang diikutsertakan memenuhi kriteria: terdiagnosis DM tipe 2, menjalani rawat inap periode tahun 2020, dan memperoleh ADO. Hasil dari proses evaluasi dipersentasekan pada masing-masing indikator rasionalitas yang diteliti dan dianalisis secara deskriptif. Hasil evaluasi menunjukan bahwa jenis-jenis ADO yang digunakan oleh sebanyak 83 pasien DM tipe 2 yang menjalani rawat inap di salah satu RS Nasional di Dili, yaitu metformin (62.5%), gliklazid (14.15%), dan kombinasi metformin dengan gliklazid (22.89%). Rasionalitas penggunaan ADO berdasarkan guideline ADA (2020) secara berturut-turut, yaitu tepat indikasi 100%, tepat pasien 100%, tepat obat 72.28%, tepat dosis 100%, dan waspada efek samping 93.97%. Walaupun pencapaian rasionalitas saat ini sudah cukup baik, namun perbaikan terhadap keterbatasan penggunaan obat di salah satu RS Nasional di Dili, Timor-Leste perlu ditingkatkan
Aktivitas Antioksidan Ekstrak Etanol Dan Fraksi Ciplukan (Physalis angulata) Pada DPPH (1,1-difenil-2-pikrihidrazil)
Get PDFAntioksidan eksogen pada tanaman diketahui memiliki efek samping yang kecil, murah dan digunakan dalam mencegah penyakit. Dengan berkembangnya penggunaan tanaman dan sayuran sebagai sumber antioksidan alami, banyak peneliti tertarik untuk menginvestigasi dan mempelajari hal ini. Dalam penelitian ini, ekstrak etanol dan fraksi tanaman ciplukan (Physalis angulata) dilakukan investigasi dan evaluasi potensi aktivitas antioksidannya. Fokus dari penelitian ini adalah menetapkan kadar dan mengevaluasi aktivitas antioksidan dari ekstrak etanol dan fraksi ciplukan.
Akar, batang, dan daun ciplukan diekstraksi dengan menggunakan metode maserasi kemudian dipartisi dengan fraksi polar (air), non polar (n-heksana), dan semipolar (etil asetat). Hasil ekstraksi kemudian dianalisis kadar flavonoid total dengan menggunakan kuersetin sebagai standar dan aktivitas antioksidan pada DPPH. Hubungan antara kadar total flavonoid dan aktivitas antioksidan ditentukan dengan metode regresi linier.
Ekstrak etanol daun ciplukan memiliki nilai total flavonoid tertinggi yaitu 38.04 ± 0.8 mg/g kemudian diikuti oleh ekstrak akar (9 ± 0.2 mg/g), dan ekstrak batang (7.1 ± 0.1 mg/g). Fraksi etil asetat dari ekstrak etanol daun ciplukan memiliki aktivitas antioksidan yang tinggi yaitu 32.10 ± 0.2 µg/ml yang dihitung sebagai IC50 kemudian diikuti oleh fraksi air (38.20 ± 0.8 µg/ml), dan fraksi heksana (38.20 ± 0.8 µg/ml) . Hasil analisis regresi linier menunjukkan aktivitas antioksidan berkorelasi dengan nilai total flavonoid dan disimpulkan bahwa flavonoid merupakan komponen antioksidan utama pada tanaman ciplukan. Exogeneous antioxidant from plant had been known have less side effect, less expensive and usually available as source to prevention disease. With rising interest and widespread of used plants and vegertables that play role as natural antioxidant many researcher interest to investigate and study these parts. In this study, ethanol extract and fraction of ciplukan (Physalis angulata) was investigated and evaluated the potential antioxidant activity. The focus in this work was to determinate of total flavonoid and evaluate antioxidant activity of ethanol extract and fraction of ciplukan.
Stem, leaves, and roots of ciplukan was extracted by using maceration method then it was partitioned with polar fraction (water), non polar (n-hexane), and semipolar (ethyl acetate). Quercetin used as the reference in the study for determinate total flavonoid content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to evaluate antioxidant activity. The correlation between total flavonoid and antioxidant activity was analyzed by using linier regression method.
The leaves ethanol extract of ciplukan was containing the highest (13.7 ± 0.9 mg/g) and followed by roots (9 ± 0.2 mg/g) and stem extract (7.1 ± 0.1 mg/g). From the fraction of leaves ethanol extract of ciplukan, the ethyl acetate fraction showed the highest flavonoid content (38.04 ± 0.8 mg/g) followed by the water fraction (15.36 ± 0.6 mg/g) and the hexane fraction (15.36 ± 0.6 mg/g). The ethyl acetate fraction from leaves ethanol extract showed the highest antioxidant activity calculated as IC50 (32.10 ± 0.2 µg/ml) then water fraction (38.20 ± 0.8 µg/ml), and hexane fraction (38.20 ± 0.8 µg/ml). The results from regression linier analysis were showed antioxidant activity was correlated to the total flavonoid content and exhibited flavonoid compound play major as antioxidant component in this plant
Pengaruh Penerapan Good Corporate Governance Dan Efektivitas Audit Internal Terhadap Kualitas Laporan Keuangan Di Organisasi Pemerintah Daerah Kabupaten Ende, Nusa Tenggara Timur
Get PDFThe purpose of this study to determine the effect of the implementation of good corporate governance and internal audit effectiveness on the quality of financial reports in the regional government organization of Ende Regency, East Nusa Tenggara, either partially or simultaneously. The population in this research are all staff/employees who work in local government organizations, especially the finance department. There are 32 registered Regional Government Organizations (OPD). The sample obtained and used in the study was 75 respondents. The sample was selected using a sample selection technique, namely Purposive Sampling. The method used is Multiple Linear Regression The results of the study are: Good Corporate Governance has a significant positive effect on the Quality of Financial Statements, Internal audit has a positive effect on the Quality of Financial Report
Penilaian Postur Kerja Pada Pekerja Bagian Penggorengan Keripik
Get PDFThe postures of frying workers when they do their activities are back bent, the body twists to the right and left, and the arm reachs too wide. The work activities are putting slices of tempe in a frying pan, and frying and draining tempe. From the interview, we know that workers felt the most pain in the buttocks/thighs and back. The pain happens because workers do their activities in a static position for a long time. It causes complaint to the skeletal muscle system. The purpose of this study are to find out the complaints that felt by workers, find out the work posture in accordance with ergonomics rules or not, and find out how big the level of risk is. The methods used in this study were Nordic Body Maps (NBM) questionnaire, Rapid Upper Limb Assessment (RULA) method and Rapid Entire Body Assessment (REBA) method. The results of the analysis using the NBM questionnaire showed that 50% workers felt pain in their shoulders, 50% in their lower back,60 % in their buttocks/thighs, and 50% in their knees. The second conclusion of the NBM questionnaire was the work posture of frying workers did not match with the ergonomics rules. The results of the RULA assessment shows the work activity of the frying department was 7. This level was very high level, while the results of the REBA assessment show a very high risk with a score of 11. Further actions needed are investigating and immediating change actions. Improvements that can be made for workers are using a chair with a backrest, doing stretching movements to reduce the pain felt in the limbs, and rearranging the work area.
Keywords: work posture at frying station, Nordic Body Maps, RULA, REBAPostur pekerja bagian penggorengan melakukan aktivitasnya dengan punggung membungkuk, tubuh memutar kekanan dan kekiri, jangkauan lengan terlalu lebar. Aktivitas kerja meliputi proses memasukkan irisan tempe pada wajan penggorengan, menggoreng dan meniriskan keripik tempe. Hasil wawancara saat observasi awal pekerja merasakan sakit paling banyak pada bagian pantat/paha serta punggung. Hal ini terjadi karena pekerja melakukan aktivitasnya dengan posisi statis dalam durasi lama sehingga menyebabkan munculnya keluhan sistem otot rangka. Tujuan dari penelitian ini adalah mengetahui keluhan – keluhan yang dirasakan pekerja, mengetahui postur kerja sesuai dengan kaidah ergonomi atau tidak, dan mengetahui seberapa besar tingkat risikonya. Metode yang digunakan pada penelitian ini adalah kuisoner Nordic Body Maps (NBM), metode Rapid Uupper Limb Assessment (RULA) dan metode Rapid Entire Body Assessment (REBA). Hasil analisis menggunakan kuisioner NBM menunjukkan bahwa pekerja merasa sakit pada bagian bahu sebesar 50%, punggung bawah sebesar 50%, pantat/paha sebesar 60%, dan lutut sebesar 50%. Kesimpulan lanjutan dari kuisioner NBM, postur kerja pekerja bagian penggorengan belum memenuhi kaidah ergonomi. Hasil penilaian dengan metode RULA menunjukkan aktivitas kerja bagian penggorengan memiliki tingkat risiko sangat tinggi dengan skor 7, sedangkan hasil penilaian dengan metode REBA menunjukkan risiko sangat tinggi dengan skor 11. Tindakan lanjutan yang diperlukan adalah investigasi dan lakukan tindakan perubahan segera/secepatnya. Perbaikan yang dapat dilakukan adalah dengan menggunakan kursi yang ada sandaran dan sesuai antropometri pekerja, melakukan gerakan peregangan untuk mengurangi rasa nyeri yang dirasakan pada anggota tubuh, dan menata ulang area kerja agar barang-barang berada dalam jangkauan.
Kata kunci: postur kerja di stasiun penggorengan, Nordic Body Maps, RULA, REB