E-Jurnal Unimor (Universitas Timor)
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ANALISIS KANDUNGAN SENYAWA KIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BATANG SIRSAK (ANNONA MURICATA L) ASAL KABUPATEN TTU TERHADAP PERTUMBUHAN BAKTERI ESCHERICHIA COLI
No full textPenyakit infeksi merupakan keadaan dimana suatu mikroorganisme masuk ke dalam tubuh dan berkembang biak sehingga menimbulkan penyakit. Faktor penyebab penyakit infeksi adalah pola hidup masyarakat yang kurang sehat sehingga menjadi pemicu kehadiran bakteri. Salah satunya adalah bakteri Escherichia coli. Penelitian ini bertujuan untuk mengetahui kandungan senyawa metabolit sekunder dan menguji aktivitas antibakteri ekstrak kulit batang sirsak asal kabupaten TTU terhadap pertumbuhan bakteri Escherichia coli. Tahapan Penelitian ini meliputi ekstraksi metabolit sekunder secara maserasi, skrining fitokimia dan yang terakhir adalah uji aktivitas antibakteri menggunakan metode difusi cakram. Berdasarkan hasil penelitian yang telah dilakukan, diketahui ekstrak kulit batang sirsak mengandung metabolit tanin, flavonoid dan triterpenoid. Hasil uji antibakteri terhadap pertumbuhan bakteri Escherichia coli dari 5 variasi konsentrasi menunjukkan bahwa ekstrak kulit batang sirsak dapat menghambat pertumbuhan bakteri. Diameter penghambatan ekstrak kulit batang sirsak secara keseluruhan masuk dalam kategori sedang dengan diameter penghambatan tertinggi berada pada konsentrasi 100% yaitu sebesar 10,66± 0,57
ANALISIS KANDUNGAN SENYAWA DAUN KAYU PUTIH (Melaleuca leucadendra L.) DAN PEMANFAATANNYA DI DESA HUMUSU SAINIUP KECAMATAN INSANA UTARA
No full textTanaman kayu putih (Melaleuca leucadendra L.) dengan potensinya sebagai tanaman penghasil minyak obat berpeluang besar untuk lebih dikembangkan dengan baik dari teknik budidaya untuk menghasilkan tanaman kayu putih dengan kandungan minyak atsiri berkualitas. Penelitian ini bertujuan untuk mengetahui senyawa daun kayu putih (M. leucadendra L.) dan pemanfaatannya di Desa Humusu Sainiup, Kecamatan Insana Utara. Metode yang digunakan dalam penelitian ini adalah metode eksprimen di Laboratorium. Penelitian ini diawali dengan pengambilan sampel kemudian sampel daun kayu putih di preparasi. Sampel daun kemudian di maserasi menggunakan pelarut metanol 96%. Hasil ekstrak dipekatkan menggunakan rotary evaporator. Analisis kandungan senyawa menggunakan alat GC-MS. Hasil analisis GC-MS menunjukkan bahwa terdapat 9 komponen senyawa. Senyawa yang paling dominan yaitu senyawa sekuiterpena dengan 3 senyawa utama yaitu, senyawa α-selinene dari golongan senyawa seskuiterpena, dengan persen area 16.07%, dengan retensi waktu 9.160 menit, senyawa R-aromadendrane dari golongan senyawa seskuiterpena, dengan persen area 18.98%, dengan retensi waktu 9.351 menit, dan senyawa β-eudesmol dari golongan senyawa seskuiterpena teroksigenasi, dengan persen area 16.14%, dengan retensi waktu 11.469 menit. Berdasarkan hasil penelitian dapat disimpulkan Terdapat 3 Komponen senyawa yang paling dominan yaitu senyawa α-selinene dari golongan senyawa seskuiterpena, senyawa R-aromadendrane dari golongan senyawa seskuiterpena dan senyawa β-eudesmol dari golongan senyawa seskuiterpena teroksigenasi
Comparative Effects of Fermentation of Sugar Kefir Grains (Tibicos) And Tetracyclin On Three Major Ileum Bacteries In Broiler Chickens
No full textAntibiotics such as tetracycline in the livestock industry face severe restrictions due to increasing antimicrobial resistance. Tibicos fermentation, as a natural probiotic, has the possibility to be an adequate and safe alternative for modulating gut microbiota. The study aimed to compare the effectiveness of tibicos fermentation and tetracycline in modulating the population of three major bacteria (Escherichia coli, Lactobacillus, and Salmonella spp.) in the ileum of broiler chickens. Ninety Indian River strain broilers were divided into three treatments with three replicates (n=10 per replicate): control with pure water (KN), 45 ppm tetracycline (KP), and 5% tibicos fermentation (FT). Treatments were given through drinking water ad libitum from 8 to 35 days of age. Bacterial populations were analyzed using the Total Plate Count (TPC) method on ileal samples aged 35 days. Data analysis used a multivariate approach including Bray-Curtis dissimilarity, PERMANOVA, Principal Coordinates Analysis (PCoA), and Similarity of Percentage Analysis (SIMPER). Bray-Curtis dissimilarity analysis showed moderate differences in microbiota composition between treatments with values of 0,064854 (FT vs KN), 0,069687 (FT vs KP), and 0,07451 (KN vs KP). SIMPER analysis identified E. coli as the primary contributor to differences between treatments, with contributions of 39,50% (KP vs KN, p=0,005), 65,70% (KP vs FT, p=0,001), and 41,10% (KN vs FT, p=0,001). Principal Coordinates Analysis showed apparent clustering between treatments, with tibicos fermentation producing microbiota profiles that differed from control and tetracycline. Tibicos fermentation showed comparable effectiveness to tetracycline in modulating ileal bacterial composition, particularly in controlling pathogenic populations while maintaining Lactobacillus stability
Identification of Gastrointestinal Nematoda on Kacang Goat (Capra aegagrus hircus) In Tiakur Moa District Southwest Maluku
No full textGastrointestinal nematodes are one of the common groups of parasites in ruminants, especially goats. These parasites are also affected livestock productivity. This study aims to identify gastrointestinal nematode parasites and prevalence of infection to kambing kacang in Tiakur, Moa District, Southwest Maluku. The samples in this study are fecal collected from 50 kacang goats that are raised semi-intensively by two local farms in Tiakur. The examination of parasites eggs was performed using the direct native method and the test results were analyzed descriptively. The results of this study reveal the prevalence of 80% gastrointestinal parasite in farm 1 and 92% in farm 2. Parasite species that are infected kacang goats in two local farms in Tiakur are Haemonchus contortus, Trichuris sp., and Trichostrongylus sp. Lack of knowledge from the farmers about the implementation of biosecurity and maintenance management has a significant impact on gastrointestinal parasite infections in goats. This study also supports the local farmers to increase and improve the knowledge and increase deworming schedules to prevent the animal from parasites infections
PEMANFAATAN LIMBAH AYAM BROILER SEBAGAI PUPUK BOKASHI PADA LAHAN HIJAUAN PAKAN TERNAK DI UPTD SONIS LALORAN
No full textHijauan pakan merupakan sumber utama nutrisi bagi ternak sapi, sehingga kualitasnya sangat dipengaruhi oleh kesuburan tanah. Tanah subur menghasilkan hijauan yang berkualitas. Kegiatan PKL ini bertujuan untuk memanfaatkan limbah ayam broiler sebagai bahan utama pembuatan pupuk bokashi untuk meningkatkan kesuburan tanah pada lahan hijauan pakan ternak di UPTD Sonis Laloran, Kabupaten Belu. Proses pembuatan pupuk bokashi melibatkan pencampuran limbah ayam broiler, hijauan seperti daun lamtoro dan daun gamal, serta larutan EM4 sebagai agen fermentasi. Fermentasi dilakukan selama 1-2 minggu dengan pengontrolan suhu dan kelembaban, hingga menghasilkan bokashi matang yang ditandai dengan aroma khas seperti tanah. Pupuk bokashi kemudian diaplikasikan pada lahan hijauan pakan, yang menunjukkan peningkatan kualitas tanah dan produktivitas hijauan. Hasil menunjukkan bahwa pemanfaatan limbah ayam broiler sebagai pupuk bokashi efektif dalam meningkatkan kesuburan tanah dan mutu hijauan pakan, untuk mendukung produktivitas ternak sapi di kawasan UPTD Sonis Laloran
PELATIHAN PEMBUATAN PAKAN TERNAK BABI DI DESA LETMAFO TIMUR
No full textDesa Letmafo Timur merupakan salah satu desa yang mendapat bantuan alat pencampuran pakan dan pencetakan pellet dari pemerintah karena desa ini memiliki potensi besar dalam sektor peternakan yang menjadi tulang pungguing bagi masyrakatnya. Namun permasalahan yang dihadapi oleh masyarakat Desa Lemafo Timur yaitu belum paham cara menggunakan peralatan pencampuran ransum hingga pencetakan pellet. Oleh karena tim akademisi dari Universitas Timor memberikan penyuluhan dan pelatihan proses pencampuran pakan hingga pencetakan pellet. Kegiatan pengadian ini dilakukan pada 27 januari 2025. Matode yang digunakan dalam pengadian ini adalah penyuluhan dan pelatihan pencampuran pakan dan pencetakan pellet. Hasil dari kegiatan pengabdian ini adalah pemcampuran pakan dan pencetakan pellet. Disampulkan bahwa dengan adanya kegiatan pengadian ini masyrakat Desa Letmafo Timur benar - benar paham tentang proses pencampuran pakan hingga pencetakan pellet.
 
Analisis Kesalahan Siswa Dalam Menyelesaikan Soal Matematika Materi Vektor Berdasarkan Taksonomi Bloom Ranah Kognitif Pada Siswa SMA
No full textThis study aims to determine the errors made by students in solving math problems, especially vector material based on Bloom\u27s Taxonomy of the cognitive domain. This research was conducted at SMA Negeri 1 Kefamenanu in the 2024/2025 school year. The subjects in this study were 30 class X students. The research instruments used were test questions and interview instruments. The test questions given were 6 numbers of questions made based on 6 levels of cognitive domain in Bloom\u27s Taxonomy. This research uses a qualitative approach method with descriptive qualitative research type, starting from data reduction, data presentation, and conclusion drawing. The results of this study indicate that the dominant errors made by students are conceptual errors. This error reflects the students\u27 lack of mastery of the concept that is the basis for solving the problem. The second error made was a technical error. This shows that although students understand what to do, they are still less careful or less careful in performing arithmetic operations. The third error made by students is procedural error. This error occurs due to irregularity or errors in the order of completion
Analysis of Land Use And Cover and its Proportion for Ruminant Farming in Lima Puluh Kota Regency
No full textThe purpose of this study was to assess the potential for ruminant livestock development in Lima Puluh Kota District by analyzing land cover and land use. A high-accuracy land cover map (kappa = 0.97) was created using Landsat 8-9 Path/Row 127/060 satellite imagery and the Random Forest (RF) classification method. Analysis was conducted using Geographic Information System (GIS) processes to evaluate land suitability. Slope, elevation, and proximity to water sources. As a result, about 35% of the district is highly suitable (S1) for ruminant farming. Due to the abundance of natural fodder and conditions conducive to extensive grazing systems, these ideal areas are mostly zones of dryland agriculture, mixed gardens and shrubs. However, due to limited fodder supply and geographical constraints, densely forested environments and urban environments are categorized as moderately suitable or unsuitable. The importance of improving livestock spatial arrangements was highlighted by the significant mismatches found when existing livestock population data were spatially overlaid with suitability maps. In conclusion, Lima Puluh Kota District has a strong biophysical basis to support ruminant livestock development. However, to ensure sustainable and effective use of land resources for livestock, spatial planning and land use policies must be aligned
Validation of Analysis Methods for Phosphorus in Chicken Meat and Fresh Cow\u27s Milk Using Microwave Destruction and Spectrophotometry Microplate UV-VIS
No full textPhosphorus content in animal-derived foods is an important indicator of nutritional value and food safety. This study validated a method for phosphorus analysis in chicken meat and fresh cow’s milk using wet digestion based on microwave digestion and microplate UV-Vis spectrophotometry with the principle of molybdenum blue complex formation by ascorbic acid at a wavelength of 880 nm. Method performance was evaluated according to ISO/IEC 17025:2017. The validation parameters included linearity and linear range, sensitivity comprising the limit of detection (LOD) and limit of quantification (LOQ), precision comprising repeatability and intermediate precision, as well as accuracy. The calibration curve showed good linearity within the range of 2–12 ppm (r² = 0.998). The LOD and LOQ values were 0.29 and 0.97 mg/L for chicken meat, and 0.17 and 0.57 mg/L for cow’s milk, respectively. Precision testing resulted in repeatability and intermediate precision RSDs of 2.00% and 2.18% for chicken meat, and 2.62% and 2.14% for cow’s milk. Accuracy, determined by recovery analysis, showed values of 97.14% for chicken meat and 103.15% for cow’s milk. These results demonstrate that the validated method meets the acceptance criteria of ISO/IEC 17025. The method provides acceptable linearity, sensitivity, precision, and accuracy, and can be applied for routine phosphorus analysis in meat and milk matrices.
Keywords: Phosphorus, chicken meat, cow’s milk, method validation, UV-Vis spectrophotometr
Analysis of Cholesterol Levels of Free-Range Chicken Meat With Turmeric Flour Supplementation
No full textThis study aimed to determine the effect of dietary turmeric (Curcuma longa) supplementation on the cholesterol profile of native chicken meat. Five treatments (control and four levels of turmeric inclusion) with five replications each were used. Data were analyzed using one-way ANOVA. The results showed that turmeric supplementation significantly affected meat cholesterol content (F = 16.302; p < 0.001). The highest cholesterol level was observed in the control group (67.06 ± 4.86 mg/100 g), while the lowest was in treatment 3 (41.57 ± 11.07 mg/100 g). Furthermore, crude protein and fat content of the meat also showed significant differences among treatments. These findings indicate that turmeric powder can be applied as a functional feed to produce native chicken meat with lower cholesterol content