Publikasi Online Fakultas Biologi UNSOED (Universitas Jenderal Soedirman)
Not a member yet
384 research outputs found
Sort by
A SIMPLE PARAFFIN EMBEDDED PROTOCOL FOR FISH EGG, EMBRYO, AND LARVAE
Get PDFThis paper describes a simple protocol of paraffin-embedded histological section for fish eggs, embryo and larvae of the hard-lipped barb and the giant gourami. The specimens were fixed in Bouin solution, washed in 70% ethanol, then were dehydrated in a series of ethanol solution of increasing concentration until absolute ethanol was reached. The specimens were cleared in graded xylene and were infiltrated with liquid paraffin then were embedded in pure paraffin. Upon sectioning, at 4–5 µm thick the specimens were attached to the gelatin-coated glass slide and let to dry at room temperature or 37°C overnight. The specimens were deparaffinized in xylene, rehydrated then were stained with hematoxylin and eosin. After being dehydrated in graded ethanol, the specimens were cleared in xylene and were mounted with an organic mounting agent. Any step in preparing histological section including samples collection, fixation, dehydration, infiltration and embedding might contribute to the quality of histological features. A proper knowledge of the tissues beeing processed, fixative solution and the histological techniques is essential to gain good results. Bouin fixative is preferable to fix fish larvae and produce a good histological feature. Decalcification is necessary to produce a good histological section on the specimens containing bone
Deteksi Resistensi Amoxicillin Helicobacter pylori pada Pasien Dispepsia
No full textAmoxicillin is one of the antibiotics that commonly used on treatment of H. pylori infection. pbp1A gene mutation in H. pylori is a dominant cause of amoxicillin resistance. This study was aimed to evaluate the presence of H. pylori in patients with dyspepsia by using non-invasive method i.e. H. pylori stool antigen (HPSA) and invasive method i.e. pbp1A gene amplification, and also evaluate the amoxicillin resistance of H. pylori by assessing the pbp1A gene mutations. The samples were 26 faeces and 26 gastric biopsies of patients with dyspepsia from the Internal Disease of Prof. Dr. Margono Soekardjo Hospital in Purwokerto. DNA amplification performed by using polymerase chain reaction (PCR) to detect the presence of amoxicillin resistance encoding gene i.e. penicillin binding protein (pbp1A). Sequencing of the DNA sample was performed at the First Base Malaysian Company, to analyze the existence of a point mutation. DNA sequencing analysis of 12 samples showed the presence of a mutations in pbp1A gene from 2 samples, in the third motive of pbp i.e. amino acid changes, Alanine 599 substituted to Threonin and Threonin 592 to Alanine. Those mutations become a dominant risk factor for resistance of H. pylori, toward the bacterial peptidoglycan synthesis. In this research, it was known that the detection of H. pylori infection by using PCR remains more accurate and specific method. The presence of H. pylori mutant strains in this study may becomes the risk factors of resistance to amoxicillin treatment.
Pertumbuhan Cabai Merah (Capsicum annuum L.) pada Tanah Masam yang Diinokulasi Mikoriza Vesikula Arbuskula (MVA) Campuran dan Pupuk Fosfat
Get PDFThis research aimed to find out the interaction effect between mixed Vesicular Arbuscular Mycorrhiza (VAM) and phosphate fertilizer to the growth of red chili (C. annuum) in acid soil, and to fnd out the best combination of mixed VAM and phosphate fertilizer to the growth of red chili (C. annuum) in acid soil. This research used an experimental method with Completely Randomized Design (CRD) in a factorial pattern with two factors. The first factor was mixed VAM dosages consisted of four levels: 0; 10; 15; 20 g/plant. The second factor was phosphate fertilizer dosages consisted of four levels: 0; 0,2; 0,4; 0,6 g/plant. Each combination treatment had three replication. The parameters were observed in the form of plant height, stem diameter, plant top dry weight, degree of VAM infection, and P content of plant tissue. Data obtained from the observation was analyzed with Analysis of Variance (ANOVA) at an error rate of 5% and 1%, treatment that showed significant or very significant result, then followed with Honestly Significant Difference (HSD) test. The result showed that interaction between mixed Vesicular Arbuscular Mycorrhiza (VAM) and phosphate fertilizer did not increase the plant height, stem diameter, and plant top dry weight, but each factor increased the plant height, stem diameter, and plant top dry weight. VAM dosage inoculation of 20 g/plant without phosphate fertilizer is the most effective combination in increasing the degree of VAM infection
Interaksi Makroalgae dan Lingkungan Perairan Teluk Carita Pandeglang
No full textMakroalgae liar adalah flora perairan laut, tumbuh berada di paparan terumbu karang. termasuk dalam Chlorophyceae. Phaeophyceae dan Rhodophyceae, tumbuh Penelitian menggunakan metode transek quadrat pada October Tahun 20016. Setiap stasiun transek ditarik garis tegak lurus pantai. Hasil penelitian makroalgae di determinasi; kekayaan jenis, densitas dan interaksi makroalgae dengan temperatur, turbiditas, pH, salinitas serta oksigen terlarut. Makroalgae yang peroleh ada 18 jenis dengan nilai indeks keanekaragaman berada di Pantai Matahari Utara 0,219, Matahari Tengah 0,24 dan Matahari Selatan 0,213. Nilai indeks kemerataan tertinggi 0,240 berada di Matahari Tengah.Interaksi kekayaan dan kepadatan jenis berpengaruh dengan stasiun (Spearman rank rs = 0,521*; rs = 0,552* ) berada Matahari Tengah dan Matahari Utara. Intaraksi tertinggi makroalagae dengan faktor suhu (spearman rank rs = 0,687** ) sangat berpengaruh terhadap jenis Eucheuma spinosum
ANTIBACTERIAL CAPACITY OF Streptomyces ISOLATE FROM A MANGROVE PLANT RHIZOSPHERE Avicennia marina
Get PDFThis research was conducted to obtain Streptomyces isolates from Avicennia marina rhizosphere capable of inhibiting E. coli and S. aureus growth, to investigate the capability and the characteristics of its antibacterial compound. This study completed the isolation by applying pour plate method on SCN agar medium. Antagonistic screening and selection processes were carried out by diffusion and dilution methods. Observation on the characteristic of the antibacterial compound applied was TLC method and MIC assay. This research confirmed the antibacterial compound capability by applying bioautography assay. Parameters measured consisted of inhibition zone diameter, Rf value on a bioautography plate, and the lowest concentration capable of inhibiting bacterial growth. Out of 16 isolates of Streptomyces obtained, Streptomyces 404 showed higher antagonistic activity than others. Inhibition zone diameter reached 20–25 mm in E. coli and S. aureus growth, respectively. TLC assay showed three spots in which two of them confirmed antibacterial activity in the bioautography assay that yielded Rf values of 0.47 for E. coli and 0.72 for S. aureus, while MIC assay showed that the lowest extract concentration inhibited bacterial growth was 20%
KEANEKARAGAMAN MIKROALGA DI SUNGAI KATI LUBUKLINGGAU
No full textWe conducted an initial survey of the microalgal diversity of Kati River located in the City of Lubuklinggau, South Sumatra Province, on March to May 2017. This study aimed to determine the microalgal diversity of Kati River as a baseline data. This study found 47 microalgal species belonging to 42 genera and 27 orders. We found the member of Chlorophyceae and Bacillariophyceae were the most abundant microalgae. We also compared the diversity of Kati River to two other rivers in Lubuklinggai, Mesat River, and Kalingi River. The microalgal diversity of Kati River is higher than Mesat River but lower if compared to Kalingi River. The physicochemical factors of Kati River waters were also measured, the average water temperature was 27°C, average pH was 6.7, and average water transparency was 57 cm. This study found that those environmental factors influenced microalgal diversity in Lubuklinggau rivers, Kati River, as well as Mesat River and Kalingi Rivers, have indicated the same results
THE FIRST REPORT OF THE DIVERSITY OF ENSIFERA (INSECTA: ORTHOPTERA) FROM ROKAN HULU DISTRICT, RIAU PROVINCE
Get PDFThe objective of this study was to determine the diversity of the suborder Ensifera in Dusun 3, Rambah Hilir Tengah, Rambah Hilir Sub-District, Rokan Hulu District, Riau Province, Indonesia. The study was conducted from September 2015 to January 2016 at six sampling locations by sweep net and hand picking at day and night hours. We recorded 17 species of the Ensifera belonging to 12 genera, 2 families, and 10 subfamilies. The diversity comprised of Conochepalus maculatus, Conochepalus melaenus, Conochepalus sp., Elbenia sp., Elimaea sp., Euscyrtus concinnus, Hexacentrus unicolor, Loxoblemus parabolicus, Mecopoda elongate, Mecopoda sp., Nisitrus vittatus, Orthelimaea sp., Podoscirtinae species 1 (unidentified), Teleogryllus emma, Teleogryllus sp., Trellius sp. and Xabea sp. The C. maculatus and E. concinnus were the predominant species in this study
ANALISIS KARAKTER TRUSS MORPHOMETRICS PADA IKAN KEMPRIT (Ilisha megaloptera) FAMILIA PRISTIGASTERIDAE
No full textThe fish auction site at Ocean Fishing Port of Cilacap is one of the major fish landings sites in Indonesia. Fishes landed in this port mainly of large pelagic fish, bycatch, crustaceans including kemprit fish (Ilisha megaloptera Swainson,1839). Kemprit fish is a member of the family Pristigasteridae, which has an elongated body shape-sprawl. Fishers use this fish as the main ingredient to produce fish crackers and dried salted fish. Overfishing is the greatest threat to kemprit fish, and without conservation efforts the future population is uncertain. Fish farming is one right answer in kemprit fish conservation. However, information on this fish biology is insufficient. One of the biological aspects is sexual dimorphism, the morphological properties that can be used to distinguish between male and female fish. Truss morphometrics can measure the morphological traits differences of male and female. This technique in which the measurement of the distance Truss morphometrics in certain parts, by the benchmark points (dots Truss morphometrics), then compared with standard size. The result of the preliminary survey indicated that kemprit fish did not show sexual dimorphism, therefore truss morphometric technique applies to distinguish sexes of this fish. This research was a survey, and purposive sampling techniques were used to collect data. Data were then analyzed using the “t” test. The results showed that distinct truss distance between male and female fish was the distance between the anterior base of the dorsal fin to the anterior base of the ventral fins and the distance between the posterior base of the dorsal fin to the anterior base of the anal fin
TINGKAH LAKU MAKAN LUTUNG JAWA Trachypithecus auratus DI KAWASAN PANCURAN 7 BATURADEN GUNUNG SLAMET JAWA TENGAH
Get PDFJavan Langur (Trachypithecus auratus) has an important ecological role in conserving the forest as their habitat by spreading seeds of fruits of their food. They are also responsible for the pattern of the plant diversity and forest regeneration as their habitat. Unfortunately, Javan Langur has been listed on Appendix II of CITES and categorized as vulnerable by the IUCN. Pancuran 7 Baturaden on the Mount Slamet slope is one of the few Javan Langur habitats in Java. Information on the Javan Langur in this area was inadequate. Therefore this study was aimed to get information on the type of food and the feeding behavior of Javan Langur, particularly in Pancuran 7 Baturaden. This study used a survey method, and the Animal Scan sampling technique was applied. The variables observed were sex ratio, population size, eating position, eating process, feeding locations, feeding duration, including the food type of the Javan Langur. Observed data for each variable were analyzed descriptively as percentages, and the results showed the Javan Langur were eating by sitting and standing, were marking trees to feed, were using hands and mouth to eat, were eating leaves and fruit, were eating on the trees and bushes. There were nine plant species as their food variety, while Matoa fruit and leave (Pometia pinnata) were their preferred foods
Karakter Antomi Daun Tumbuhan Mangrove Akibat Pencemaran di Hutan Mangrove Kabupaten Cilacap
Get PDFThis research was aimed to observe the anatomical character of mangrove plants leave and examine the differences from leaves anatomical character of mangroves in Donan river and Tritih forest in Cilacap regency as an self-adaptation indicator. The method which is used was survey method by collecting leaves sample of each mangrove plants. The sample was preparated using embedding method, and stained with safranin 1% disolved in alcohol 70%. The parameter of variable which is observed in leaves anatomical character are thickness of leaves, leaves mesophyll thickness, cuticle thickness, stomata length size, and amount of stomata and trichome per leaves unit area. The data were analyzed with t-test for examining anatomical character difference. The results showed that four usual types of mangrove plants such as Achantus ilicifolius, Rhizospora mucronata, Aegiceros corniculatum, and Bruguiera gymnorrhiza have similar leaves anatomical structure. The anatomical character from polluted habitat were different from unpolluted habitat. This differences was showed in leaves mesophyll thickness, cuticle thickness, stomata length size, and amount of stomata and trichome per mm2 leaves unit area.