Scientific Journals of Bogor Agricultural University
Not a member yet
25587 research outputs found
Sort by
Corrigendum: Status Terkini Penyakit Tristeza Pada Tanaman Jeruk di Sulawesi Tenggara. : Corrigendum: Status Terkini Penyakit Tristeza Pada Tanaman Jeruk di Sulawesi Tenggara.
No full textCorrigendum: Taufik M, Nurulita S, Yusuf DN, Miftahkuhrohmah, Botek M, Gusnawaty HS, Syarni E. 2025. Status Terkini Penyakit Tristeza Pada Tanaman Jeruk di Sulawesi Tenggara. Jurnal Fitopatologi Indonesia. 21(1):28-37. DOI: https://doi.org/10.14692/jfi.21.1.28-37.In the original version of this article, the affiliation of the author Miftahkuhrohmah was incorrectly stated as: “National Research and Innovation Agency, Jalan Raya Bogor KM 46, Bogor 16915”. The correct affiliation is: “Research Center for Estate Crops, National Research and Innovation Agency, Jalan Raya Jakarta–Bogor km 46, Cibinong, Bogor, West Java, 16911”. This correction does not affect the substance, data, or conclusions of the article. The correction has been made in the online version and the PDF version of the article.Corrigendum: Taufik M, Nurulita S, Yusuf DN, Miftahkuhrohmah, Botek M, Gusnawaty HS, Syarni E. 2025. Status Terkini Penyakit Tristeza Pada Tanaman Jeruk di Sulawesi Tenggara. Jurnal Fitopatologi Indonesia. 21(1):28-37. DOI: https://doi.org/10.14692/jfi.21.1.28-37.
Pada versi asli artikel ini afiliasi penulis Miftahkuhrohmah semula tertulis sebagai berikut:“Badan Riset dan Inovasi Nasional, Jalan Raya Bogor KM 46, Bogor 16915”. Afiliasi penulis dipebaiki menjadi “Pusat Riset Tanaman Perkebunan, Badan Riset dan Inovasi Nasional, Jalan Raya Jakarta–Bogor km 46, Cibinong, Bogor, Jawa Barat, 16911”. Perubahan ini tidak memengaruhi substansi, data, maupun kesimpulan artikel. Perbaikan telah dilakukan di situs web dan versi artikel PDF
Coral Reef Transplantation Towards Ecotourism on Pramuka Island
Full text linkIndonesia is a country with high biodiversity. However, Indonesia also has the second highest rate of biodiversity decline in the world. Coral reef ecosystems are highly productive ecosystems that play an important role in terms of ecology and economy. One of the ecological conservation efforts that have been carried out by the community in the tourism sector on Pramuka Island is implementing the concept of ecotourism. Ecotourism is tourism with environmental and educational principles, and can be realized in natural environment restoration activities. One of the coral reef ecosystem restoration activities that has long been carried out on Pramuka Island is coral transplantation. 39% of tourists were satisfied with the concept of ecotourism. These tourists stated that the concept of ecotourism was appropriate to be applied in society, because it was considered capable of improving many sectors such as tourism and the economy, as well as improving ecological conditions for the better in a sustainable manner. There are several communities on Pramuka Island that are involved in the ecotourism sector, one of which is the Smiling Coral Indonesia (SCI) group. One of SCI\u27s flagship programs is coral reef restoration based on sustainable tourism. Ecotourism can strengthen the community\u27s economic sector, one of which is creating jobs. This means that ecotourism has two important roles that have good potential to be developed. Ecotourism has a positive impact on the ecosystem and is one of the community\u27s economic resources
Hepatoprotection and Immunomodulation of Natural Killer and CD8 T Cells by Meniran-Turmeric Extract Combination in Mice Injected with 7,12-Dimethylbenz[a] Anthracene
Full text linkCombination herbal or polyherbal offers advantages by synergizing multiple plants to address health issues more effectively. Meniran and turmeric are traditionally used medicinal plants with notable pharmacological activities due to their bioactive compounds. This study investigated the curative effect of a meniran-turmeric extract combination on immunocompetent cells, particularly NK and CD8 cells, and on liver histopathology in mice administered 7,12-dimethylbenz[a]anthracene (DMBA). Female BALB/c mice (Mus musculus) were injected subcutaneously with DMBA at 45 mg/kg body weight (BW) in the mammary gland area and maintained for eight weeks. They were then treated with a 1:1 meniran-turmeric extract combination for two weeks at doses of 100, 300, 900, and 1,800 mg/kg BW, followed by liver and immune cell analyses. The results showed a lower CD8+ T-cell population in extract-treated groups (17.53±1.13%-19.79±1.51%) compared with the DMBA group (20.92±4.74%). Conversely, NK+ cell populations increased after extract treatment (0.33±0.01%-0.56±0.13%) compared with DMBA alone (0.27±0.02%). Liver histopathology indicated that extract combination administration did not cause more severe damage than that observed in the DMBA group. An extract combination of meniran and turmeric could modulate the immune system, particularly NK and CD8 cells, in mice injected with DMBA. In addition, the study indicated that the combination of meniran and turmeric extract might play a role as an antihepatotoxic agent. This research could serve as a consideration for future studies developing a combination formulation of meniran and turmeric
Preparation and Characterization of P(3HB-co-HHX)/Silk Fibroin Nanofiber for Chondrogenic Differentiation on Human Adipose Mesenchymal Stem Cell
Full text linkThis study explored the proliferative and chondrogenic differentiation capacities of nanofiber containing silk fibroin hybrid Bombyx mori silkworm cocoons (Japan-China SP-01 variant from Indonesia) and PHA P(3HB-co-3HHx) on human adipose mesenchymal stem cells. First, the scaffolds were prepared for electrospinning by combining two distinct biomaterials, consisting of silk fibroin derived from hybrid Bombyx mori silkworm cocoons, with a combination of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx). The effects of various ratios of P(3HB-co-3HHx)/Silk Fibroin nanofiber mixture on proliferative and differentiation capacity were then investigated. Following that, the morphology, chemical compositions, contact angle, tensile strength, roughness, cell viability, and human adipose mesenchymal stem cell differentiation of the nanofiber were investigated by collagen type 2 gene expression. The results of scanning electron microscopy showed the mean diameter of the nanofiber ranged from 370-600 nm. Following that, 16,000 volts was prominent for nanofiber manufacture in all ratios. It was also demonstrated that the nanofiber has significant mechanical properties, acceptable hydrophilicity and smoothness, and appropriate cell viability (up to 99.1% compared to the control on silk fibroin nanofiber). Although PHA increased tensile strength, silk fibroin administration to the mixture predominantly enhanced chondrogenic differentiation, as evidenced by modulation of chondrogenic collagen type 2 (up to 8.718-fold) gene markers. Furthermore, the physicochemical characteristics of the nanofiber mixture significantly influenced the proliferation and differentiation of human adipose mesenchymal stem cells. The results of the tests showed that silk fibroin administration into a nanofiber mixture has improved chondrogenesis and showed great potential as a cartilage tissue scaffold
Characterization of Lignin Biosynthesis Encoding Gene, SiCOMT, from Nine Indonesian Foxtail Millet (Setaria italica (L.) P. Beauv.) Genotypes
Full text linkCaffeic acid O-methyltransferase (COMT) is an essential enzyme that catalyzes the biosynthesis of lignin monomer units. Foxtail millet (Setaria italica) possesses three COMT-encoding genes. However, only SiCOMT1 and SiCOMT2 are considered expressed. This study investigates the characteristics of the two COMT-encoding genes across nine Indonesian foxtail millet genotypes. Phylogenetic analysis revealed that SiCOMT1 is closely related to N-methyltransferase genes, which are not involved in the lignin biosynthesis pathway. Meanwhile, SiCOMT2 is closely related to O-methyltransferase genes involved in lignin biosynthesis. SiCOMT2 from nine Indonesian foxtail millet genotypes exhibits 15 synonymous and three non-synonymous SNPs. SiCOMT2 amino acid showed Ala67Thr and Pro72Ala variations within the methyltransferase dimerization domain, and Glu146Asp within the O-methyltransferase domain. Among these, the Pro72Ala substitution is predicted to reduce the structural stability of the encoded protein. These findings suggest that SiCOMT2 may serve as a promising target for future genetic research and crop improvement strategies aimed at enhancing biomass quality by modifying lignin content and composition
Phenetic Study of Tabernaemontana divaricata (L.) R.Br. ex Roem. & Schult. in Thailand
Full text linkPinwheel Jasmine (Tabernaemontana divaricata L.; Apocynaceae) is widely distributed across South Asia, Thailand, and southern China and exhibits substantial morphological variation. This variability has resulted in persistent taxonomic confusion, particularly in Thailand, where distinguishing among phenotypic forms and related taxa remains challenging. Accurate taxonomic delimitation is essential for botanical classification, horticultural applications, conservation, and the reliable identification of plant resources. This study examined the taxonomic status of Pinwheel Jasmine in Thailand using numerical taxonomic approaches, including cluster and discriminant analyses. A total of 40 morphological characters (20 quantitative and 20 qualitative) were assessed across 210 operational taxonomic units (OTUs) representing seven phenotypes. Both analyses consistently resolved the OTUs into two distinct groups: (1) T. divaricata ‘Ceylon Jasmine’ (Phud Supachok) and (2) other Pinwheel Jasmine forms. Five quantitative characters were identified as the most diagnostic: lamina length (LML), lamina width (LMW), corolla lobe length (CLL), distance from the corolla tube base to stamen attachment (DCS), and style length (STL). These traits exhibited exceptionally high F-values in K-means cluster analysis (404.11, 432.90, 327.54, 530.04, and 181.02, respectively; p<0.01), indicating strong discriminatory power. The results demonstrate that Phud Supachok is consistently and clearly morphologically distinct from other Pinwheel Jasmine forms. However, because this study is based solely on phenetic data, additional molecular evidence is required before definitive taxonomic conclusions can be made. Integrative studies combining molecular and morphological data are recommended to refine species delimitation within Tabernaemontana
Desain primer DNA ikan sapu-sapu (Pterygoplichthys sp.) serta aplikasinya pada pempek menggunakan real time PCR (RT-PCR): Primer design of suckermouth catfish (Pterygoplichthys sp.) DNA and their aplication in pempek using real Time PCR (RT – PCR)
No full textPempek merupakan salah satu jenis makanan khas dari Sumatra Selatan yang umumnya menggunakan bahan baku ikan tenggiri. Ikan sapu-sapu juga bisa dijadikan bahan baku pembuatan pempek namun beberapa penelitian melaporkan bahwa ikan sapu-sapu yang diambil dari Sungai Ciliwung telah tercemar logam berat dan mikroplastik. Adanya kandungan logam berat dan mikroplastik pada ikan sapu-sapu menjadikan ikan ini berbahaya untuk dijadikan bahan baku pembuatan makanan khususnya pempek. Tujuan penelitian ini, yaitu mendesain primer DNA ikan sapu-sapu serta menganalisis keberadaan kandungan DNA ikan sapu-sapu pada pempek menggunakan RT-PCR dengan penanda berupa dye (EvaGreen). Analisis DNA ikan sapu-sapu menggunakan primer spesifik dan diamplifikasi menggunakan RT-PCR. Hasil penelitian menunjukkan bahwa primer yang digunakan spesifik hanya untuk ikan sapu-sapu saja bila dibandingkan dengan sampel 9 DNA ikan yang lain. Konsentrasi DNA ikan sapu-sapu sebesar 0,00064 ng/µL masih menunjukkan adanya amplifikasi dengan nilai Cq sebesar 38,98. Pada perbandingan antara bahan baku pembuatan pempek dengan daging ikan sapu-sapu (b/b) sebesar 20:1 masih menunjukkan adanya kurva amplifikasi dengan nilai Cq sebesar 23,55. Sebanyak 16 sampel pempek yang digunakan tidak mengandung DNA ikan sapu-sapu karena tidak menunjukkan adanya kurva amplifikasi pada alat RT-PCR.Pempek is a traditional food from South Sumatra that uses fish as the main ingredient. In addition to mackerel, several other types of fish are used as raw materials for making pempek, such as snakehead fish, tilapia, and catfish. Suckermouth catfish can also be used as an ingredient for making pempek; however, several studies have reported that suckermouth catfish from the Ciliwung River are contaminated with heavy metals and microplastics. The presence of heavy metals and microplastics in suckermouth catfish makes it dangerous to use as a food ingredient, especially in pempek. This study aimed to design specific DNA primers for suckermouth catfish and analyze the presence or absence of suckermouth catfish DNA in pempek using RT-PCR with a dye-based marker (EvaGreen). Suckermouth catfish DNA was analyzed using specific primers and amplified using RT-PCR. The results showed that the primers used were specific only to suckermouth catfish compared to DNA samples from nine other fish species. A suckermouth catfish DNA concentration of 0.00064 ng/µL still showed amplification, with a Cq value of 38.98. A comparison between raw pempek ingredients and suckermouth catfish meat (w/w) at a ratio of 20:1 still showed an amplification curve with a Cq value of 23.55. None of the 16 pempek samples tested contained suckermouth catfish DNA, as no amplification curves were observed on the RT-PCR device
Unlocking the Private Sector Role in Supporting the Sustainable Multipurpose Forest Management in Riau, Indonesia
Full text linkThis study aims to discover the private sector’s perspective on the sustainable transition of degraded forestlands, including the transformation of oil palm plantations in forest areas into multipurpose forests, identify current barriers in adopting sustainable multipurpose forest management practices on financing and policy aspects, and explore how the private sector can step up its role in forestland restoration. This study was based on field observations, key informant interviews, focus group discussions (FGDs), and literature reviews. This study aims to navigate a path for policy implementation toward decarbonization, as tenurial conflicts, particularly between oil palm plantations and forest areas, are critical for sustainable forest management in Riau. The private sector\u27s interest in sustainable multipurpose forest management is higher when additional benefits from non-timber forest products (NTFPs) are high. This study also found that the private sector’s desire to support sustainable multipurpose forest management stems from the potential benefits of carbon trading. Regarding the carbon market, most respondents are willing to join when carbon prices are USD4–6 ton-1 of CO2e. It indicates that the private sector is willing to support the domestic carbon market as regulated under the Minister of Environment and Forestry Regulation 21/2022. While the private sector has complied with most transformative policies and mechanisms, respondents expect further incentives and support, particularly to resolve the forestland conflict
Wood Waste Charcoal Briquettes: Physical and Thermal Characteristics Based on Particle Size and Wood Type
Full text linkThe depletion of fossil fuel reserves and rising fuel costs have highlighted the need for alternative household energy sources. Biomass waste, particularly sawmill residues, has potential for conversion into solid fuel in the form of charcoal briquettes. This study evaluated the physical and thermal characteristics of charcoal briquettes produced from Bus wood (Melaleuca sp.) and Rahai wood (Acacia sp.) sawmill waste at particle sizes of 10, 30, and 60 mesh. Briquettes were produced through carbonization, size reduction, binder mixing, molding, and drying, followed by evaluation of physical and thermal properties. The results showed that Bus wood briquettes had a higher density (0.56–0.60 g/cm³) than Rahai wood briquettes (0.46–0.58 g/cm³). Moisture content ranged from 10% to 13%, with the highest value observed in Rahai briquettes at 60 mesh, which affected combustion stability. Thermal analysis indicated that Bus briquettes at 60 mesh achieved a high peak temperature (608 ᵒC) and a high combustion time efficiency (92.31%), while Rahai briquettes exhibited a higher peak temperature at a coarser particle size (10 mesh, 604 ᵒC). Overall, Bus briquettes with finer particle size showed favorable physical properties and combustion behavior. These findings indicate that Bus wood waste briquettes have potential for further development as household-scale solid fuel.
Keywords: charcoal briquettes, briquette properties, particle size, wood waste biomassThe depletion of fossil fuel reserves and rising fuel costs have highlighted the need for alternative household energy sources. Biomass waste, particularly sawmill residues, has potential for conversion into solid fuel in the form of charcoal briquettes. This study evaluated the physical and thermal characteristics of charcoal briquettes produced from Bus wood (Melaleuca sp.) and Rahai wood (Acacia sp.) sawmill waste at particle sizes of 10, 30, and 60 mesh. Briquettes were produced through carbonization, size reduction, binder mixing, molding, and drying, followed by evaluation of physical and thermal properties. The results showed that Bus wood briquettes had a higher density (0.56–0.60 g/cm³) than Rahai wood briquettes (0.46–0.58 g/cm³). Moisture content ranged from 10% to 13%, with the highest value observed in Rahai briquettes at 60 mesh, which affected combustion stability. Thermal analysis indicated that Bus briquettes at 60 mesh achieved a high peak temperature (608 ᵒC) and a high combustion time efficiency (92.31%), while Rahai briquettes exhibited a higher peak temperature at a coarser particle size (10 mesh, 604 ᵒC). Overall, Bus briquettes with finer particle size showed favorable physical properties and combustion behavior. These findings indicate that Bus wood waste briquettes have potential for further development as household-scale solid fuel.
Keywords: charcoal briquettes, briquette properties, particle size, wood waste biomas
Utilization of mung bean sprout waste Vigna radiata hydrolyzed cellulase enzyme in feed on the digestibility of Nile tilapia Oreochromis sp.
Full text linkThis study evaluated the utilization of mung bean sprout waste flour hydrolyzed by cellulase enzyme (LTe) as the feed ingredient of red Nile tilapia weighing 10.00 ± 0.01 g/seed and 7.00 ± 0.15 in length. This study used two stages, each consisting of four treatments and four replications. The first step was performed by evaluating LTe flour added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of LTe and growth performance on red Nile tilapia seeds. The results showed that the addition of cellulase enzyme at a 1.2 g/kg was significantly able to reduce the crude fiber of LTe with 78.19% %, hemicellulose at 19.22%, neutral detergent fiber at 41.69%, acid detergent fiber at 61.85%, lignin 64.06%, cellulose 62.47% besides having the best value of ingredient, protein, and energy digestibility. The test results on the growth performance of red Nile tilapia seeds fed with LTe feed with a dose of 1.2 g/kg cellulase enzyme have the highest value significantly different from the control feed based on the value of daily growth rate (SGR), ratio efficiency protein (REP), protein retention (PR), and improvement of feed conversion ratio (RKP).
Keywords: cellulase enzyme, digestibility, growth performance, mung bean sprout waste, tilapia
Abstrak
Penelitian ini mengevaluasi pemanfaatan tepung limbah kecambah kacang hijau yang dihidrolisis enzim selulase (LTe) sebagai bahan baku pakan pada benih ikan nila merah dengan bobot 10,00 ± 0,01 g/ekor dan panjang 7,00 ± 0,15 cm. Penelitian ini menggunakan dua tahap dan masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Tahap pertama dilakukan evaluasi tepung LTe sebesar 0 g/kg (kontrol), 0,4 g/kg, 0,8 g/kg, dan 1,2 g/kg. Tahap kedua dilakukan uji kecernaan bahan LTe dan kinerja pertumbuhan benih ikan nila merah. Hasil penelitian menunjukkan bahwa penambahan enzim selulase pada dosis 1,2 g/kg signifikan mampu menurunkan serat kasar LTe sebesar 78,19 %, hemiselulosa 19,22%, neutral detergent fiber 41,69%, acid detergent fiber 61,85%, lignin 64,06%, selulosa 62,47% dan memberikan nilai tertinggi terhadap kecernaan bahan, kecernaan protein, dan kecernaan energi. Hasil uji terhadap kinerja pertumbuhan benih ikan nila merah yang diberi pakan LTe dengan enzim selulase dosis 1,2 g/kg memiliki nilai tertinggi berbeda nyata terhadap pakan kontrol berdasarkan nilai laju pertumbuhan harian (SGR), retensi protein (PR), rasio efisiensi protein (REP) dan perbaikan nilai rasio konversi pakan (RKP).
Kata kunci: enzim selulase, ikan nila merah, kecernaan, kinerja pertumbuhan, limbah kecambah kacang hija