Animal Production (E-Journal, JAP)
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The Effects of The Oxytocin Hormone Induction on The Value of The Composition of Etawah Crossbred Goat Milk
Full text linkThis study aims to see the extent of influence of oxytocin hormone induction on the nutritional composition of milk in the Etawah Crossbred goat. This research was conducted in December 2018 in Syiah Kuala District, Banda Aceh, Indonesia. The examined parameters were the levels of fat, nonfat dry matter (BKTL), protein content, lactose content and milk density. The research used 18 Etawah goats and oxytocin hormone. A statistical unpaired t test indicated a highly significant influence (P 0.05) in milk, i.e. 8.39%, 3.91% and 3.76%, respectively. In conclusion, hormone oxytocin injection had a highly significant effect on increasing milk fat levels and significantly affected the decrease in milk density but did not affect the level of nonfat dry matter (BKTL), lactose and protein
Effect of Chloramphenicol on Rabbit Doe Fertility and Prenatal Development
Full text linkChloramphenicol (CP) is a synthetic antibiotic with large spectrum, mostly used in human and veterinary medicine to fight against diverse infections. In the present study the effects of this antibiotic on some does reproductive parameters were evaluated on 24 nulliparous, sexually mature female (6 months). The animals were divided into 4 groups of 6 does each, comparable in terms of body weight. To each group was randomly attributed by gavage 25, 50 and 75mg of CP/kg of body weight 30 days before mating (6 females for 1 male) and during the whole gestation period. After sacrifice of the does on the 28th day post-coitum, the organs (ovaries, liver, and kidneys) and fetus were collected and were examined to detect eventual anomalies. The main results showed a non-significant increase (P>0.05) of the relative weight of the liver and kidneys and a non-significant decrease (P>0.05) of that of the ovaries with increase in the dose of CP. No abortions were registered no matter the dose of CP. Gestation index, the rate of fetal mortality and of fetal viability were not significantly affected by the dose of CP used. A decreasing (P0.05) of the average weight of the fetus and their body measurement, the number of corpus luteum and the sex-ratio (M/F) were registered from treated animals in relation to the control animals. A dose-dependent increase (P<0.05) of number of pre and post-implantation resorptions were noted. The only anomaly registered was the insufficient fusion of the cranial bones at the dose 50 and 75mg/kg of CP. A significant decrease (P<0.05) of the level of proteins in the serum and ovaries were noted in animals treated in relation to those of the control group. It was concluded that CP administered at doses of 25, 50 and 75 mg/kg bw to pre and post-coital rabbit does, negatively affects fertility but has no significant teratogenic effects. Its use should therefore be limited or prohibited in husbandry
Influence of Different Vegetable Oils on In Vitro Ruminal Fermentability and Nutrient Digestibility in Ettawah Crossbred Goat
Full text linkThis research was aimed to determine the effect of supplementation of vegetable oil (corn oil, palm oil and kapok seed oil) on in vitro ruminal fermentability and nutrient digestibility. Experiment design used was Randomized Block Design with four treatments and four replication based on rumen fluid sampling time. The four treatments namely R0 = feed without oil supplementation; R1 = R0 + 5% corn oil; R2 = R0 + 5% palm oil; and R3 = R0 + 5% kapok seed oil. The result showed that supplementation of 5% vegetable oil did not affect (P>0,05) the rumen pH, A/P ratio, and efficiency of energy conversion. The total VFA, acetate, propionate, butyrate, methane and NH3 was higher (P<0,05) in supplementation of 5% vegetable oil than he control. The population of protozoa, microbial protein, in vitro dry matter digestibility (IVDMD), organic matter digestibility (IVOMD), and crude fiber digestibility (IVCFD) was lower (P<0,05) in supplementation of 5% compared to the control. Population of protozoa decreased until 58,76% for R1; 66,89% for R2; and 43,33% for R3. It can be concluded that supplementation of 5% vegetable oil decreased the population of protozoa, increased the production of VFA and NH3. Supplementation of 5% kapok seed oil resulting the highest of total VFA, acetate, propionate, butyrate and NH3 among other treatments
The Characteristics and Quality of Egg from Commercial Laying Hens Fed with Garlic (Allium Sativum) Supplemented Ration
Full text linkThe research aimed to determine the characteristics and quality of egg of commercial laying hens fed ration supplemented with garlic (Allium sativum) powder. The research used Completely Randomized Design (CRD) with five treatments and four replicates (5 hens each) for a total of 100 brown laying hens strain MB 402. Garlic powder contained 24.62% crude protein, 38.81% fat, and 1.74% crude fiber. The treatments of garlic powder ration were 0, 2, 4, 6, and 8%. The treatments performed were dietary, with R0 = 100% based diet (BD); R1= 98% based diet (BD) + 2% garlic meal (GM); R2= 96% based diet (BD) + 4% GM, R3 = 94% based diet (BD) + 6% GM, R4 = 92% based diet (BD) + 8% GM. The study was conducted during eight (8) weeks. Collected data were quality, weight, shell weight, albumen weight and shell thickness of eggs. Data was analyzed with Analysis of Variance (ANOVA) and continued with Duncan’s Multiple Range Test (DMRT). The results showed that 8% garlic powder ration increased weight and albumen weight of eggs. There were no significant effect of treatments in yolk weight, shell weight and shell thickness of eggs. Garlic powder supplementation on laying hen ration affected egg weight and albumen weight. The addition of garlic powder on laying hen ration of up to 4% increased egg weight. Albumen weight increased until garlic powder supplementation of up to 8%. It is recommended the use of 8% garlic powder supplementation on lying hen ration without negative effects on egg quality
Effect of Storage Conditions on The Characteristics and Composition of Fresh Goat Cheese Containing Probiotics
Full text linkThe objective of this study was to determine the characteristics and composition of soft cheese manufactured from goat milk, which contained probiotic bacteria, and stored at different temperatures for up to 90 days. Soft cheese was manufactured from fresh Indonesian Etawah goat milk, with a mix starter culture containing Lactobacillus casei and Bifidobacterium longum (1:1). Animal rennet was added to facilitate curding. Fresh cheese produced was wrapped in linen clothes and stored under two different temperature conditions, which were high temperature (H:13-15oC) and low temperature (L: 8-10oC) for up to 90 days. Results showed that goat cheese was characterized as soft with mild goaty flavor, contained no less than 108 CFU of probiotic lactic acid bacteria, pH of 4.6, 0.5% free fatty acid, 57% moisture, crude 15% crude protein, 22% total fat and 2.5% mineral. Cheese underwent dehydration during storage which caused an increase in the relative proportion of protein, fat and mineral. The evolution of cheese’s pH, titratable acidity and composition was partly affected by storage temperature. At the end of storage periods (90 days) goat cheese was characterized as semi hard and, on average, contained 1.3-1.5% titratable acidity, pH of 4.94-4.95, 31.22-37.06% moisture, 24.59-24.09% crude protein, 33.51-36.17% total fat, and 5.64-5.53% minerals.. Cheese stored at high temperature has slight growth of mold at its surface, which was anormal condition during for cheese ripening. In conclusion, acceptable characteristics and composition of probiotic-containing cheese can be manufactured from milk of Indonesian Etawah goats and stored at temperature of 8-10oC for 90 days
The Correlation of The Age and Length of Stay with The Compliance of Beef Cattle Farmers Norms: Case Study in Final Waste Disposal of Jatibarang, Semarang City
Full text linkThe study was conducted in the final waste disposal (TPA) Jatibarang, Kedungpane, Mijen, Semarang. A survey was conducted, targeting the beef cattle farmers who pastured their cattle in the TPA Jatibarang, Semarang. The observed variables were age (X1), education background (X2), length of stay (X3), total cattle owned (X4), and the farmer’s norm compliance (Y1). Purposive sampling was used to determine the sample of regions. Total respondents were determined by simple random sampling, selecting 70 out of 350 farmers (20%). The result of the analysis showed that 78.57% farmers were at their productive age (20-55 years old), 64.30% had elementary education background, 74.29% had been farming for over 14 years, and 62.86% had fewer than seven cattle. The farmers also showed a sound moral compliance. The result of spearman rank showed a non-significant correlation between age and length of farming experience with norm compliance. Age was negatively correlated with norm compliance, but education background and total cattle owned were significantly correlated with norm compliance, showing a correlation value of 0,248* and 0,610*, respectively
The Effect of Various Legume Herbs Supplementation on Intake, Digestibility and Growth of Fat-tailed Lambs Given Mulato Grass
Full text linkThis experiment was conducted to examine the effect of various legume herbs supplementation on feed intake, digestibility and liveweight gain of fat-tailed lambs given Mulato (Brachiaria mulato) grass. Twenty four male fat-tailed lambs (8 months of age and 12.73± 0.56 kg (SE) of initial liveweight) were randomly allotted to four treatment groups based on unfasted liveweight. Animals were housed in individual metabolism crates. The experiment was designed in a completely randomized block design, with four treatments and six replicates. The dietary treatment tested included Mulato grass ad libitum (M), M + Centrosema pascuorum  (MCP), M + Dolichos lab lab (MDL) and M+ Clitoria ternatea (MCT). All supplement was offered at 1.5% body weight (W) per day. The experiment lasted for 10 weeks, with 2 and 8 weeks for adaptation and measurement period, respectively. The main parameter measured included feed intake, feed digestibility, and average daily liveweight gain (ADG). Results showed that total DM intakes (DMI) were not affected (P>0.05) by legume herbs supplementation. Total DMI was 2.75, 2.79, 2.84 and 2.87 %W/d for lamb treated with M MCP, MDL and MCT respectively. In contrast, supplementation with various legume herbs significantly increased (P 0.05) between legume herbs. Feed DMD was 58.17, 67.48, 64.91; 65.03% and ADG were 35.67; 58.39; 54.31 and 54.41 g/day, for lamb treated with M, MCP, MDL and MCT respectively. It was concluded that supplementation of legume herbs to fat-tailed lamb fed Mulato grass significantly increased DMD and ADG, but no significant differences across the legume herbs as feed supplements were observed
Antimicrobial Activity of Goat Colostrum Against Bacterial Strains Causing Food Poisoning Diseases
Full text linkThe study was aimed to investigate the antimicrobial activity of bacterial isolates L.plantarum 3CT7 and 20CT8 from goat colostrum. The antimicrobial activity of cell-free supernatant was tested using a well-diffusion method on several indicators: temperature, time of storage, and pH. Antimicrobial activity was recorded in both isolates at pH 2.0; 4.0; 6.0 and 8.0, temperature at 0, 50 and 100 oC, and in cold storage for 0, 15, 30, 45 and 60 days. L.plantarum 7CT3 and L.plantarum 20CT8 have a bigger zone of inhibition than that of Pseudomonas spp. as compared to other bacteria. Testing the cell-free activity was aimed to investigate the metabolite inhibition by L.plantarum. The isolates were capable of inhibiting all pathogenic bacteria in the experiment (S. thypimurium, E. coli, and S. aureus) as evidenced from the similar zone of inhibition from 15.83 to 16.06 mm. Isolates (L. plantarum 7CT3 dan 20CT8) exhibit inhibitory properties against S.thypimurium, S. aureus, Pseudomonas spp.. and L. monocytogenes at 0, 50 and 100oC. L.plantarum 7CT3 and L.plantarum 20CT8 exhibit antimicrobial activity during cold storage. Both isolates grown in the range of pH from 2 to 8 could inhibit S. thypimurium, E. coli, S. aureus and Pseudomonas spp. In general, the two isolates are the potential antimicrobial activity with broad ranges of pH, temperature and storage time
The Influence of Breed and Type of Extender on the Quality of Bull Semen
Full text linkThis study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender
Polymorphism of Myostatin Gene (MSTN) Coding Region in Batur Sheep
Full text linkThe aim of present study was to investigate myostatin gene polymorphism and its association with weaning weight and body weight in Batur sheep, 30 heads of Batur all lambs were fed by concentrated feed. Body weight measured monthly after weaning to the six months of age. DNA Extraction used 200 ul of whole blood samples. To amplify exon 3 region of MSTN gene a specific primer designed using the Primer3 software. The 25 μl volume contained 25 ng of genomic DNA, 12.5 μl 2x Reaction mix of each primer. The cycling protocol was 5 minutes at 95°C as initial denaturation, 35 cycles of denaturing at 94°C for 45 seconds, annealing at 73.9 for 45 seconds, extending at 72°C for 40 s, with final extension at 72°C for 10 minutes. Eleven polymorphic sites were observed in the in 3rd exon region transversions at c.*121 G instead of A, del-T at c.*129, one individual at c.*139 and one individual at c.*158 positions however, one individual sequence disrupted reading frame in whole MSTN sequenced, also weaning weight and 6-month weight of Batur lambs were 21.13±5.70 and 31.64±7.06 to the homozygous whilst 19.99±4.33 and 30.50±5.44 for heterozygote respectively but there is no significe differences seen above all the studied parameters. Homozygous AA lambs had fairly low weaning weight and body weight compared to the heterozygous AB lambs but might have a higher carcass weight, further investigation is needed into the interaction of MSTN with other genes involved in muscle growth