Kufa Journals System University of Kufa
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Detection of E. coli, as a contaminant of minced meat in certain locations of Baqubah city-Diyala Province-Iraq
This survey investigated the prevalence of Escherichia coli (E.coli)in raw minced beef meat collected from different areas of Baqubah city in Diyala Province for detection of contamination with E.coli. A(90) samples were collected at (10)samples from each area in Baqubah city.and different dilutions were made for each one (10-1 , 10-2 , 10-3 ,10-4 ).These sample were pour onto MacConkey Agar and thenstreak onto eosin methylene blue agar(EMB) plates.The results was shown presence of high contamination ratio of the microbial isolation from minced beef meat of E.coli bacteria in different areas of Bagubah city. with positive ( 76.85% ),while negative (23.1%)
Detection of Salmonella Spp. in milk samples of selected regions of Diyala city
The aim of this study was to identify milk contamination that produced from dairy cattle with salmonella organisms in different regions of Diyala city. Fresh (unpasteurized) and heat treated (pasteurized) milk samples (n=10) were collected randomly from non-similar five regions of Diyala city that well-known with dairy cattle production particularly local shops and cattle shepherd markets, samples were send to the laboratory for some microbial testing. Special selective media were prepared and cultured with pasteurized and raw unpasteurized milk, the results refered that there were infected milk samples out of 10 fresh unpasteurized milk as the following percentage, 10% Rashdiah, 20% Bani saad, 80% Ghalbiah, 50% Khalis and 20 % Shiftah, while the pasteurized milk samples displayed contamination out of 10 samples as the previous (20% Rashdiah and 10% Ghalbiah), other pasteurized samples regions showed negative results for salmonella in regions of Bani saad, Khalis and Shiftah. We concluded that both heat treated and fresh milk may be unsafe for humankind ingesting if produced under unsanitary conditions or insufficient pasteurization
Molecular Detection of Some A.hydrophila Toxins and its Antibiotics Resistance Pattern Isolated From Chicken Feces in Thi-Qar Province (Iraq)
The high isolation rate of virulent A.hydrophila from chicken represent an important public health concern especially when these bacteria exhibit increased antimicrobial resistance to medically important antibiotics, so this study was conducted to isolate A.hydrophila from chicken feces and investigate the presence of some toxins genes and determine their antibiotics resistance profile. Chicken feces were collected from different regions, throughout Thi-Qar province south of Iraq, in period extended from July 2015 to January 2016. A.hydrophila isolates were identified by morphological, biochemical and API20E. These isolated were subjected to PCR assay for confirmation, targeting 16S RNA-23SRNA Intergenic Spacers Region and for detection of important virulence genes including hemolytic toxin Aerolysin (Aero), Heat labile enterotoxin (Alt) and Heat stable enterotoxin (Ast). Twelve A.hydrophial were isolated and identified to species level among 23 Aeromonas spp isolated from chicken fecal samples with overall incidence rate (52.6%). Screening for virulence genes revealed that 10/12 (83.4%) were positive for Aerolysin ( Aero) gene and 9/12( 75%) for Heat labile enterotoxin (Alt ) gene, while none of these isolates were positive for Heat stable enterotoxin (Ast) gene 0/12(0%).The most prevalent genotype was (Aer+ Alt+ Ast_). Antibiogram against 19 antibiotics revealed that, all isolates in showed absolute susceptibility(100%) to Gentamycin, Oflaxacin, Amikacin, Norfloxacin, Imipnem Ciprofloacin. However, multidrug resistance recorded in all isolates, 2 isolates (16.7%) were resistant to eight antibiotics including , Clindamycin, Cephalothin , Vancomycin, Ticacillin-clavulnoc acid, Ceftazidime, Cefoxitin, Trimethoprim-sulfamethaxazon, Azithromycin ,with multidrug resistance index (0.42) , and 5/12 (41.7%) were resistance to seven antibiotics (58.4%) with MDRI( 0.36), four isolates( 33.4%) were resistant to six antibiotics with MDRI ( 0.31), while only one isolates 1/12(8.4%) were resistant to five antibiotic with MDRI (0.26). The present study showed that detection of pathogenic A.hydrophila harboring important virulence genes Aerolysin and Alt with resistance to many clinically important antibiotics is a good indication that chicken feces constitute important source for pathogenic Ahydrophila infecting human that come in contact with chicken and spread of multidrug resistance organisms in environment
Effect of Chitosan Supplementation to drinking water in productive performance, physiological and micro flora traits of broiler
This study carried out in the farms of animal production department /College of Agriculture /University of Tikrit during the period from 15/2/2014 to 22/3/2014. It aims to investigate effect of adding chitosan to the drinking water on the productive performance and some physiological characteristics and intestinal micro flora of broiler. A total of 150 unsexed broiler chinks at 21 day of old divided to five treatments by three replicates (10 per each ) , the first treatment without any adding ( control treatment ), in the 2ed ,3ed , 4th and 5th treatment was adding chitosan to drinking water by concentration (100,150,200 and 250 mg/l) respectively It has been found a significant increase (P<0.05) in the average weekly live body weight for the fifth week, and the total average of live body weight gain for T3 and T4 and there have not any significant differences in average consumption feed ; and a significant improvement (P<0.05) in the average of feed conversion ratio for T2,T3 and T4 . Any significant differences have not been recorded in the RBCs numbers, Hb concentration PCV% , hetrophil cells WBCs , the Cells , lymphocyte cells , H/L ratio proportion , and the concentration of globulin , Cholesterol , glucose , uric acid , ALT and AST enzymatic blood. In addition a significant decrease (P<0.05) in the total protein concentration for T3 compared with control treatment , albumin concentration for T3 compared with T5 , total number of bacteria for T2 , and bacteria number of lactobacillus acid for T3 . In addition, a significant decrease (P<0.05) in the triglyceride concentration for T3 and E.coil bacteria number for T3 compared with control treatment. Concludes from this study the water soluble chitosan improved broiler performance and intestinal micro flora without any effect of physiological status
Comparative microscopically study of the skin in local and wild rabbits
This study was prepared to compare between the skin of local and wild rabbits. The samples were taken from five body region (ear, abdomen, neck, thorax and back ).The present study revealed that the basic structure of the skin in local and wild rabbits formed of supperfacial layer is epidermis, which covered with keratinized stratified squamous epithelium and arranged into four rows from cells, beside thickness of epidermis in local rabbit more than that thickness of epidermis in wild rabbits .The deep layer is dermis which dermis divided into two layers, the superficial papillary and deep reticular layers without a clear line demarcation between two layers. Local rabbit dermis was thicker than that wild rabbit dermis due to density of dense irregular connective tissue and fibers. There are two types of hair follicles in both animals was primary and secondary hair follicles. The follicles consists of one into three primary hair follicles and clusters of secondary hair follicles in local rabbit whereas in the wild rabbits, the follicles consists of central primary hair follicles and several of smaller secondary follicles surrounded the central primary hair follicles. The present study revealed that ratio of secondary to primary hair follicles was less in local rabbits than that wild rabbits
Docetaxel Chemotherapy Induced Peripheral Neuropathy in Breast cancer Patients and its Amelioration by Vitamin E
Background: Chemotherapy is used to treat different types of cancer. Its use is associated with neurotoxicity, the most common of them is peripheral neuropathy. Taxanes are recognized to cause peripheral neuropathy of which Docetaxel is studied. Aim: To evaluate the neurotoxicity of Docetaxel and assess the protective effect of vitamin E in breast cancer patients. Methods: In a prospective placebo controlled randomized study, 60 women with breast cancer were selected. Patients had surgery and planned for chemotherapy administration. Chemotherapy protocol used is TAC that includes Docetaxel, Doxorubicin and Cyclophosphamide given every 21 days. They are assessed for neuropathy clinically and by nerve conduction study at the baseline and after completion of chemotherapy. They are divided into 2 groups each composed of 30 patients. The 1st group is given Vitamin E and the second group is given placebo. Results: showed that the incidence of neuropathy is the same in both groups, but it was less severe in Vitamin E treated group compared to the placebo group. Conclusion: vitamin E ameliorates the severity of peripheral neuropathy and can be used for this purpose. Recommendation: We recommend large multicenter studies to be done and using other agents that possibly prevent or ameliorate Docetaxel induced neuropathy in the future
ميزاتاليهودفيرواية«حكومة الظل» لمنذر القباني (دراسة نقدية اجتماعية)
إنّ اليهودهممنالقوىالكبرىفيالعالمويحاولونالسيطرةعلىالعالمو هيمنتهوإيقاعالمسلمينفيمستنقعالجهلواللاأخلاقي. رواية «حكومة الظل» لمنذر القباني من رواياتتعكسهذهالقضيةوقادرةعلىاستيعابملامحهذهالظاهرة. استهدفنا في هذه المقالة من خلال دراسة نقدية اجتماعية متبنّيةً علىالمنهجالوصفيـالتحليلي، إلقاء الضوء على كيفية حضورهم و المحاولة لاستيلائهم علىالمجتمعالإسلامي لكي يصبحواهمالملك الحقيقيفيالعالم. منالنتائجالتيوصلناإليهاأن الكاتب تحدّث عن اليهود في المحاور الثلاثة و حذّر المسلمين و العالم كلّه من خططهم و مذاهبم نحو الماسونية و الدونمة و جمعيّة الاتّحاد و الترقي كما أنّ القبّاني شرح للقرّاء بشكل غير مباشر خصائص اليهود و خاصة الماسونية نحو السرّية، و المال و الرموز و العُنف و محاولتهم لانتشار الشهوة و قد أعلن الكاتب عبر روايته خطرهم في المجتمع الإسلامي. و من جهة أخرى هناك دعوة غير مباشر في هذه الرواية لكلّ مسلمٍ يقرأها و يشجّعهم الكاتب علىالتمسّكبالقرآنالكريموالمثقفين الذينيحولوندونهم نحو جمال الدينالأفغاني
الأفارقة الأمريكان والحرب العالمية الثانية1939-1945
شارك الافارقة الامريكان في الحرب العالمية الثانية 1939-1945م مشاركة فاعلة , كان وراء مشاركتهم هذه آمال عريضة بالحصول على حقوقهم كمواطنين من الدرجة الاولى , بعد الممارسات التمييزية التي تعرضوا لها من قبل الحكومة والمجتمع على حد سواء , لكن على الرغم من ذلك عانى الافارقة الامريكان من نفس المعاملة العنصرية حتى داخل المؤسسة العسكرية , مما ادى الى تزايد حدة المطالب ,كما بدأوا بتنظيم انفسهم بشكل اكبر للحصول على الغاية المنشودة في الحرية والمساواة
دور التخطيط العمراني في حماية البيئة الحضرية من النفايات المنزلية (دراسة حالة حي المكرمة في النجف الاشرف)
يهدف البحث الى دراسة مشكلة ادارة النفايات المنزلية في حي المكرمة احد احياء مدينة النجف الاشرف ووضع الحلول لهذهالمشكلة, وقد ركزت الدراسة على دور التخطيط العمراني في حماية البيئة الحضرية من النفايات المنزلية,ثم حساب وتقدير كمية النفاياتالمنزلية المتولدة ومعرفة النسب المئوية لمكونات المخلفات الصلبة, ولأجل تحقيق هذا الهدف تم إجراء المسح الميداني واعداد استمارة استبيان لتحديد تركيب ومعدل انتاج النفايات الصلبة المنزلية لمجموعة من الآسر التي تم انتخابها في منطقة الدراسةوقد بينت الدراسة انكميةالنفاياتاليوميةالتيتنقلمنالحي والمنطقةالتجارية تقريباهي 46 طنيوميا , بينما كمية النفايات المطروحة و المقدرة حوالي 57,706 طن/يوم ، أي أن هناك ما يقارب 11,706 طن/يوم من النفايات لا يتم رفعها من الشوارع و الساحات الخالية المفتوحة في الحي, حيث يتم رمي النفايات في الساحات و الفضاءات المكشوفة وتحويل الأرصفة إلى مكبات للنفايات لعدم وجود حاويات تجمع فيها النفايات ,وهو أمر يؤدي إلى تجمع الحشرات والقوارض التي تؤثر صحياً على سكان المدينة فضلا عن تشوه جمالية الحي, ومما يزيد من حجم المشكلة هو قله الكوادر البشرية المتمثلة بعمال النظافة وقلة سيارات جمع ونقل النفايات فضلاً عن عدم إتباع الطرق الصحية للتخلص من النفايات
The relationship between Reactive Oxygen Species and Insulin resistance of Obese individuals in Babylon /Iraq
The aim of this study was to evaluate the relationship between Reactive Oxygen Species and Insulin resistance the measured by homeostasis model assessment of insulin resistance (HOMA index) at Obese individuals in Babylon /Iraq, who do not suffer from chronic diseases. This study was performed in department of nutrition at the Murjan teaching hospital / in Babylon province/Iraq, in April 2016. This study included 90 (100%) were patients with obesity52(57.78%) and 38(42.22%) were control for age range from (19-40 years) and mean± S.D for BMI of obese group (36.00±4.36) and BMI of control (22.86±1.67). The data demonstrated that the obese groups, had higher significant differences (P≤ 0.05) in the Body max index (BMI) (Kg/M2), FBG (mmol/L) and HOMA index, whereas there is no significant differences of the Age (years) and the concentrations of Insulin(ng/ml) ,ROS(ng/ml) comparison with control groups. The results revealed that no significant differences in the Age(year), FBG (mmol/L), insulin(ng/ml), HOMA index and ROS(ng/ml) at the three class of obesity. Correlation analysis showed that a significant positive correlate (y = 12* + - 106 ROS, r = 0.30, P = 0.05) between Reactive oxygen species(ng/ml) with HOMA index of obese group at classes III, whereas there is no significant correlate between Reactive oxygen species(ng/ml) with HOMA index of obese group at classes I and II .