JKI Open Journal Systems (Julius Kühn-Institut)

Julius Kühn-Institut

JKI Open Journal Systems (Julius Kühn-Institut)
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    Sektion 49 Bienen und andere Bestäuber

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    49-1 - Einfluss von bienengefährlichen Pflanzenschutzmitteln auf Bienenvölker im Frühjahr: Ein Monitoring in Südtiroler Apfelanlagen (2014 - 2017)Benjamin Mair, Manfred Wolf 49-2 - Cyantraniliprol: Auswirkungen eines neuen Insektizides auf Honigbienen und andere Bestäuber infolge verschiedener AnwendungsformenAxel Dinter 49-3 - Untersuchung des Übertrags von Pflanzenschutzmittelrückständen in Larvenfutter von Königinnen und Arbeiterinnen der Honigbiene (Apis mellifera L.) unter HalbfreilandbedingungenAlexandra Bölling, Jakob H. Eckert, Gabriela Bischoff, Robert Kreuzig, Jens Pistorius 49-4 - Auswirkungen von Tankmischungen auf HonigbienenAnna Wernecke, Malte Frommberger, Jens Pistorius 49-5 - Effekte und Auflagen: Risikobewertung der Bienengefährlichkeit von TankmischungenNadine Kunz, Abdulrahim T. Alkassab, Anna Wernecke, Ina Patrizia Wirtz, Jens Pistorius 49-6 - Rückstände in bienenrelevanten Matrizes nach Applikation mit DroplegULPeter Trodtfeld, Reinhard Friessleben, Christian Maus 49-7 - Abdrift von Beizstäuben: Zusammenfassende Ergebnisse einer neunjährigen FeldversuchsreiheAndré Krahner, Udo Heimbach, Gabriela Bischoff, Matthias Stähler, Jens Pistorius49-1 - Impact of the use of plant protection products harmful to bees on bee colonies during spring: Results of a monitoring programme in apple orchards in South Tyrol (2014-2017)Benjamin Mair, Manfred Wolf 49-2 - Cyantraniliprole: Effects of a new insecticide on honey bees and other pollinators following different usesAxel Dinter 49-3 - Investigating the transfer of pesticide residues in royal jelly and worker jelly of honey bees (Apis mellifera L.) under semi-field conditionsAlexandra Bölling, Jakob H. Eckert, Gabriela Bischoff, Robert Kreuzig, Jens Pistorius 49-4 - Effects of tank mixtures on honey beesAnna Wernecke, Malte Frommberger, Jens Pistorius 49-5 - Effects and Mitigation measures: risk assessment on hazards of tankmixture to beesNadine Kunz, Abdulrahim T. Alkassab, Anna Wernecke, Ina Patrizia Wirtz, Jens Pistorius 49-6 - Residues in bee-relevant matrices after application with DroplegULPeter Trodtfeld, Reinhard Friessleben, Christian Maus 49-7 - Emission of abraded dust particles: Lessons learned from a 9-year field-experimental seriesAndré Krahner, Udo Heimbach, Gabriela Bischoff, Matthias Stähler, Jens Pistoriu

    Vorrats- und Nachernteschutz

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    058 - Untersuchung zum mikrobiellen Befall von Lagerzwiebeln (Allium cepa)Sebastian Wunder, Ute Gärber058 - Investigation of the microbial infestation of storage onionsSebastian Wunder, Ute Gärbe

    Diagnose- und Nachweisverfahren

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    123 - Etablierung eines molekularen on-site Testverfahrens zur Diagnose von Phytoplasmen an Reben und ObstkulturenHolger Linck, Sven Keil, Frank Brändle, Annette Reineke 124 - Viren, Viroide und Phytoplasmen in Reben und Obstbäumen - Detektion von Mehrfachinfektionen mittels HochdurchsatzsequenzierungKerstin Zikeli, Constanze Berwarth, Dennis Knierim, Christoph Hoffmann, Michael Maixner, Stephan Winter, Wilhelm Jelkmann 125 - Vorkommen der Ulmenvergilbung in einheimischen Ulmenarten in DeutschlandBernd Schneider, Marlies Karaus, Heidrun Mattauch, Michael Kube 126 - Validierung des Ködertests mit Rhododendronblättern für ein routinemäßiges Monitoring von Probenmaterial auf Kontamination mit Phytophthora ramorum nach der EPPO-Richtlinie PM7/98 (2)Corina Junker, Sabine Werres 127 - Charakterisierung pilzlicher Schaderreger an Kamille (Matricaria recutita L.)Monika Götz, Katja Sommerfeld, Samad Ashrafi, Stefan Wagner, Ute Gärber 128 - In situ Immunfluoreszenz-Lokalisierung: Eine Methode zur einfachen Detektion von Beauveria spp. in BodenprobenCornelia I. Ullrich, Frank Rabenstein, Eckhard Koch, Natascha Heil, Marta Matek, Regina G. Kleespies 129 - Nachweis von Schildlausarten (Coccoidea) in BrandenburgUte Schönfeld123 - Development of an on-site molecular diagnostic tool for phytoplasmas in grapevine and other speacialty cropsHolger Linck, Sven Keil, Frank Brändle, Annette Reineke 124 - Application of next-generation sequencing for simultaneous detection of viruses, viroids and phytoplasmas in grapevine and fruit treesKerstin Zikeli, Constanze Berwarth, Dennis Knierim, Christoph Hoffmann, Michael Maixner, Stephan Winter, Wilhelm Jelkmann 125 - Occurrence of Elm Yellows Phytoplasma in native Elm Species in GermanyBernd Schneider, Marlies Karaus, Heidrun Mattauch, Michael Kube 126 - Validation of the bait test with rhododendron leaves for routine monitoring of samples for contamination with Phytophthora ramorum according to the EPPO Guideline PM7/98 (2)Corina Junker, Sabine Werres 127 - Characterisation of fungal pathogens of chamomille (Matricaria recutita L.)Monika Götz, Katja Sommerfeld, Samad Ashrafi, Stefan Wagner, Ute Gärber 128 - In situ immunofluorescence localization: A method for the rapid detection of Beauveria spp. in soil samplesCornelia I. Ullrich, Frank Rabenstein, Eckhard Koch, Natascha Heil, Marta Matek, Regina G. Kleespies 129 - Detection of scale species (Coccoidea) in BrandenburgUte Schönfel

    Molekulare Phytomedizin / Virologie / Bakteriologie / Mykologie

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    130 - Organisation von Laborvergleichsuntersuchungen für PflanzenvirenWulf Menzel, Stephan Winter 131 - Einsatz eines reversen genetischen Systems bei Rizomania zum besseren Verständnis der Rz1 ResistenzüberwindungSebastian Liebe, Edgar Maiss, Mark Varrelmann 132 - Molekulare Charakterisierung unterschiedlicher TRV-Herkünfte und Analyse der Wechselwirkungen von Virus, Nematode und Kartoffelsorte als Basis für die ResistenzzüchtungKerstin Lindner, Johannes Hallmann, Christina Hieronymus, Hans-Reinhard Hofferbert, Bernd Truberg4, Silke Wagener, Stefan Lütke Entrup 133 - Herstellung von infektiösen cDNA Volllängenklonen des Strawberry mild yellow edge virus (SMYEV) und Übertragungsversuche mit der Erdbeerhaarknotenlaus (Chaetosiphon fragaefolii)Wilhelm Jelkmann, Constanze Berwarth 134 - Ausbreitung von rekombinanten Plum Pox Potyvirus (PPV-Rec)-Stämmen in SüdwestdeutschlandWolfgang Jarausch, Miriam Runne, Alexandra Baßler, Nannu Molla 135 - Nachweis zweier neuartiger Viren in Birke an urbanen und natürlichen Standorten in EuropaKaja Pack, Maria Landgraf, Bright Opoku, Martina Bandte, Susanne von Bargen, Artemis Rumbou, Martin Schreiner, Barbara Jäckel, Carmen Büttner 136 - Virologische Untersuchungen an erkrankten Birken im Raum BerlinElisha Bright Opoku, Maria Landgraf, Martina Bandte, Susanne von Bargen, Martin Schreiner, Barbara Jäckel, Carmen Büttner 137 - Untersuchungen zur Verbreitung eines neuartigen Emaravirus in Sämlingen der Stieleiche (Quercus robur L.)Marius Rehanek, Franziska Otto, Susanne von Bargen, Martina Bandte, Carmen Büttner 138 - Untersuchungen zum Auftreten eines neuartigen Virus der Esche (Fraxinus excelsior) und Vervollständigung der RNA1-SequenzThomas Gaskin, Susanne von Bargen, Martina Bandte, Jean-Sébastien Reynard, Hans-Peter Mühlbach, Jenny Roßbach, Carmen Büttner 139 - Mikrobielle Antagonisten gegen das EschentriebsterbenKristina Ulrich, Regina Becker, Andreas Ulrich, Michael Kube 139a - Das Rhizosphärenmikrobiom als potentieller Initiator der gesteigerten Resistenz in GersteNina Bziuk, Desirée Lauterbach, Adam Schikora, Kornelia Smalla 140 - Ein einfacher und schneller Nachweis für Tilletia controversa, dem Verursacher des Zwergsteinbrands von WeizenSomayyeh Sedaghatjoo, Monika K. Grundler, Andreas J. Geissler, Ludwig Niessen, Petr Karlovsky, Berta Killermann, Wolfgang Maier 141 - Peronospora-Arten an SalviaMascha Hoffmeister, Marco Thines, Wolfgang Maier 142 - Molekulare Charakterisierung von Ascochyta fabae Isolaten, Erreger der Ascochyta-Blattfleckenkrankheit der AckerbohneSai Vivek Chinna Peketi, Stefanie Remer, Wolfgang Link, Birger Koopmann130 - Organization of proficiency tests for plant virusesWulf Menzel, Stephan Winter 131 - Application of a reverse genetic system to understand Rz1 resistance breaking in the rizomania disease complexSebastian Liebe, Edgar Maiss, Mark Varrelmann 132 - Molecular characterization of different Tobacco rattle virus (TRV) field strain isolates and analyses of interactions between virus, nematode and potato cultivar to establish the basis for TRV resistance researchKerstin Lindner, Johannes Hallmann, Christina Hieronymus, Hans-Reinhard Hofferbert, Bernd Truberg4, Silke Wagener, Stefan Lütke Entrup 133 - Production of full length infectious cDNA clones of Strawberry mild yellow edge virus (SMYEV) and transmission trials with the strawberry aphid (Chaetosiphon fragaefolii)Wilhelm Jelkmann, Constanze Berwarth 134 - Spread of recombinant Plum Pox Potyvirus (PPV-Rec)-strains in Southwest GermanyWolfgang Jarausch, Miriam Runne, Alexandra Baßler, Nannu Molla 135 - Detection of two novel virus variants in birch of urban and native origins in EuropeKaja Pack, Maria Landgraf, Bright Opoku, Martina Bandte, Susanne von Bargen, Artemis Rumbou, Martin Schreiner, Barbara Jäckel, Carmen Büttner 136 - Investigation of viral diseases in declining birch trees in BerlinElisha Bright Opoku, Maria Landgraf, Martina Bandte, Susanne von Bargen, Martin Schreiner, Barbara Jäckel, Carmen Büttner 137 - Studies on the distribution of a novel emaravirus in seedlings of Common oak (Quercus robur L.)Marius Rehanek, Franziska Otto, Susanne von Bargen, Martina Bandte, Carmen Büttner 138 - Study to Occurance of a new Virus in European Ash (Fraxinus excelsior) and Completion of the RNA1 SequenceThomas Gaskin, Susanne von Bargen, Martina Bandte, Jean-Sébastien Reynard, Hans-Peter Mühlbach, Jenny Roßbach, Carmen Büttner 139 - Microbial Antagonists Against Ash DiebackKristina Ulrich, Regina Becker, Andreas Ulrich, Michael Kube 139a - The rhizosphere microbiome as potential initiator for enhanced resistance in barleyNina Bziuk, Desirée Lauterbach, Adam Schikora, Kornelia Smalla 140 - Simple and rapid detection of Tilletia controversa causing dwarf bunt in wheat seedsSomayyeh Sedaghatjoo, Monika K. Grundler, Andreas J. Geissler, Ludwig Niessen, Petr Karlovsky, Berta Killermann, Wolfgang Maier 141 - Peronospora-species on SalviaMascha Hoffmeister, Marco Thines, Wolfgang Maier 142 - Molecular characterisation of Ascochyta fabae isolates, causal agent of ascochyta blight on faba beansSai Vivek Chinna Peketi, Stefanie Remer, Wolfgang Link, Birger Koopman

    Herbologie / Unkrautbekämpfung

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    161 - Untersuchungen zur Cytochrom P450-basierten Nicht-Zielort-Resistenz bei Apera spica-ventiDagmar Rissel, Lena Ulber 163 - Herbizidkontrolle verschiedener Amarant-Arten in Mais und ZuckerrübenHans-Peter Söchting 164 - Unkrauterkennung mittels Flugroboter auf AckerflächenHenning Nordmeyer, Michael Schirrmann, Christina Wellhausen, Michael Pflanz161 - Studying cytochrome P450-based non-target site resistance in Apera spica-ventiDagmar Rissel, Lena Ulber 163 - Herbicide control of different Amaranth-species in maize and sugar beetHans-Peter Söchting 164 - Drone based weed monitoring on arable landHenning Nordmeyer, Michael Schirrmann, Christina Wellhausen, Michael Pflan

    IWCSPP, Past Conferences and Proceedings

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    Table of contents

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    Insect infestation sources in stored maize grain; what is more important resident versus incoming infestation?

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    Most studies targeted pest control inside stores; incognisant of the population dynamics in the store vicinity; leading to product re-infestation. Distinction between storage insect pest source and sink grain patches is important for effective pest management strategies. We examined the role of resident versus incoming insect infestation in phosphine-fumigated closed or open and unfumigated closed or open maize farm stores. Grain quality measurements were recorded over 32 weeks for two storage seasons. Whether open or closed, fumigated grain had significantly lower (p < 0.001) grain damage and lower grain weight loss (p < 0.05) than unfumigated grain. Fumigated open stores had significantly higher (p= 0.004) grain damage and weight loss than closed ones. Grain damage was higher in unfumigated-closed than fumigated-open, evidence that resident infestation inflicted higher food loss than incoming infestation. Prostephanus truncatus, Cryptolestes ferrugineus and Tribolium castaneum had significantly higher populations (p < 0.001, p = 0.018 and p = 0.001; respectively) at bottom levels of unfumigated and fumigated grain (T. castaneum). Sitotroga cerealella and Sitophilus zeamais were significantly higher (p < 0.001) at the top of closed than open unfumigated compartments. Grain suffers less infestation and quality loss when it is a sink patch than when it is a source patch. Population build-up and ‘settling’ to inflict significant food loss takes longer for incoming compared to resident infestation. These results have ecological implications on postharvest IPM.Most studies targeted pest control inside stores; incognisant of the population dynamics in the store vicinity; leading to product re-infestation. Distinction between storage insect pest source and sink grain patches is important for effective pest management strategies. We examined the role of resident versus incoming insect infestation in phosphine-fumigated closed or open and unfumigated closed or open maize farm stores. Grain quality measurements were recorded over 32 weeks for two storage seasons. Whether open or closed, fumigated grain had significantly lower (p < 0.001) grain damage and lower grain weight loss (p < 0.05) than unfumigated grain. Fumigated open stores had significantly higher (p= 0.004) grain damage and weight loss than closed ones. Grain damage was higher in unfumigated-closed than fumigated-open, evidence that resident infestation inflicted higher food loss than incoming infestation. Prostephanus truncatus, Cryptolestes ferrugineus and Tribolium castaneum had significantly higher populations (p < 0.001, p = 0.018 and p = 0.001; respectively) at bottom levels of unfumigated and fumigated grain (T. castaneum). Sitotroga cerealella and Sitophilus zeamais were significantly higher (p < 0.001) at the top of closed than open unfumigated compartments. Grain suffers less infestation and quality loss when it is a sink patch than when it is a source patch. Population build-up and ‘settling’ to inflict significant food loss takes longer for incoming compared to resident infestation. These results have ecological implications on postharvest IPM

    Larvae of Trogoderma respond behaviorally to whole body extracts: Presentation

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    Behavioral responses to semiochemicals by Trogoderma (Coleoptera: Dermestidae) stored product pests were assayed in a small arena. Hexane extracts were obtained from Khapra beetle, Trogoderma granarium, warehouse beetle, Trogoderma variable, and the larger cabinet beetle Trogoderma inclusum that were killed by being frozen for 48 hours at -20° C. These extracts were analyzed using gas chromatography coupled with mass spectrometry (GC/MS), and it was confirmed that they contain several cuticular hydrocarbons, fatty acids and sterols. Two choice experiments were performed inside Petri dish arenas, with filter paper fully covering the bottom surfaces. Two smaller 3cm filter papers were placed on opposite ends within each arena. Each of the smaller papers were folded three times in parallel to present a corrugated surface that the insects could move underneath if they chose. In each case, one paper had a 100µl aliquot of one of the extracts, and the other 100µl of hexane as a control. 10 late instar larvae of the same species as the treatment extract were placed in the arena and allowed to acclimate overnight in a dark room. For all three species, it was found that larvae were more likely to be found on the side of the Petri dish with the hexane control rather than the conspecific larval extract. They were also more likely to be on or near the smaller corrugated filter paper treated with the control as opposed to the filter paper treated with the larval extract. Thus repellency of the conspecific extract was demonstrated at that particular dose. Further assays using different doses of the raw extracts and their individual chemical components are planned. The use of these semiochemicals in novel management strategies will be considered.Behavioral responses to semiochemicals by Trogoderma (Coleoptera: Dermestidae) stored product pests were assayed in a small arena. Hexane extracts were obtained from Khapra beetle, Trogoderma granarium, warehouse beetle, Trogoderma variable, and the larger cabinet beetle Trogoderma inclusum that were killed by being frozen for 48 hours at -20° C. These extracts were analyzed using gas chromatography coupled with mass spectrometry (GC/MS), and it was confirmed that they contain several cuticular hydrocarbons, fatty acids and sterols. Two choice experiments were performed inside Petri dish arenas, with filter paper fully covering the bottom surfaces. Two smaller 3cm filter papers were placed on opposite ends within each arena. Each of the smaller papers were folded three times in parallel to present a corrugated surface that the insects could move underneath if they chose. In each case, one paper had a 100µl aliquot of one of the extracts, and the other 100µl of hexane as a control. 10 late instar larvae of the same species as the treatment extract were placed in the arena and allowed to acclimate overnight in a dark room. For all three species, it was found that larvae were more likely to be found on the side of the Petri dish with the hexane control rather than the conspecific larval extract. They were also more likely to be on or near the smaller corrugated filter paper treated with the control as opposed to the filter paper treated with the larval extract. Thus repellency of the conspecific extract was demonstrated at that particular dose. Further assays using different doses of the raw extracts and their individual chemical components are planned. The use of these semiochemicals in novel management strategies will be considered

    Susceptibility of dried berries to infestation by Plodia interpunctella (Lepidoptera: Pyralidae) in correlation with total sugar content: Poster

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    By assessing the degree of resistance of stored products to infestation by insect pests and correlating it with physical, chemical and nutritional characteristics of products, we could gain a real insight in these pests feeding preferences, and consequently in their biology and ecology. The aim of this study was to assess the degree of resistance of five dried berry species (strawberry, raspberry, blackberry, black chokeberry and cranberry) to infestation caused by the major pest of dried berries, Plodia interpunctella. Susceptibility was rated based on the Index of susceptibility (IS) for insect development and the Susceptibility rating. Dried cranberries were absolutely resistant to infestation by P. interpunctella (IS = 0) - no larvae reached the adult stage. Four other dried berry species were also resistant (IS ranged 2.01 – 2.44). In other words, dried cranberries are very unsuitable food for P. interpunctella, while other four tested species were slightly more suitable. The content of total sugars in dried berries varied from 24.2% (black chokeberry) to 72.8% (strawberry), but important correlation between IS and total sugar content was not found. By analysing feeding preferences of P. interpunctella, we can undertake different pest-management strategies for protection of stored dried fruits.By assessing the degree of resistance of stored products to infestation by insect pests and correlating it with physical, chemical and nutritional characteristics of products, we could gain a real insight in these pests feeding preferences, and consequently in their biology and ecology. The aim of this study was to assess the degree of resistance of five dried berry species (strawberry, raspberry, blackberry, black chokeberry and cranberry) to infestation caused by the major pest of dried berries, Plodia interpunctella. Susceptibility was rated based on the Index of susceptibility (IS) for insect development and the Susceptibility rating. Dried cranberries were absolutely resistant to infestation by P. interpunctella (IS = 0) - no larvae reached the adult stage. Four other dried berry species were also resistant (IS ranged 2.01 – 2.44). In other words, dried cranberries are very unsuitable food for P. interpunctella, while other four tested species were slightly more suitable. The content of total sugars in dried berries varied from 24.2% (black chokeberry) to 72.8% (strawberry), but important correlation between IS and total sugar content was not found. By analysing feeding preferences of P. interpunctella, we can undertake different pest-management strategies for protection of stored dried fruits

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