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"Adaptation of maize-based food-feed-energy systems to limited phosphate resources" (AMAIZE-P) – a new Sino-German international research training group.
In vitro assessment of anti-diabetic potential of 4 kinds of dark tea (Camellia sinensis L.) protein hydrolysates
The contributions of four kinds of dark tea (Camellia sinensis L.) proteins and their hydrolysates to hypoglycemic activity were investigated in vitro. Four kinds of water-extracted dark tea proteins were hydrolyzed with trypsin and Alcalase, respectively. The complete proteins had α-amylase inhibitory activity with half maximal inhibitory concentration (IC50) values ranging from 1.27 to 2.78 mg/mL. Most of the dark tea proteins and hydrolysates significantly inhibited α-glucosidase and dipeptidyl peptidase (DPP-IV), with IC50 values in the range of 0.0103-1.3114 mg/mL and 0.1000-1.3364 mg/mL, respectively. In general, Heimaojian (HMJ) and Qianliang (QL) hydrolysates displayed high α-glucosidase inhibitory activity, while HMJ, Fuzhuan (FZ), and Heizhuan (HZ) hydrolysates exhibited a strong ability to inhibit DPP-IV. This study demonstrates the potential of dark tea proteins and their hydrolysates as a source of functional food and medicine for the control of type 2 diabetes
Nutritional composition and genetic diversity of Thai Aromatic Rice landraces
Fifty Thai aromatic rice landraces and one commercial cultivar (KDML 105) were subjected to proximate nutritional analysis to determine protein, fat, fiber, carbohydrate, ash, moisture, amylose and 2AP contents. Genetic diversity was characterized using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Fifty-one cultivars were clustered based on RAPD and ISSR markers. Various nutrient compositions of the fifty rice landrace cultivars and one commercial cultivar were checked. Among 100 RAPD primers tested, 15 showed high polymorphism (100%) with an average of 21.2 bands per primer. Fifteen ISSR primers out of 100 produced high polymorphism (99.74%) with an average of 26 bands per primer. The UPGMA dendrogram based on genetic similarity grouped the cultivars into two clusters and several sub-clusters. Clustering of aromatic rice using ISSR markers gave increased clarity and was more effective than using RAPD markers for both nutritional composition and polymorphism level. Findings will provide practical guidelines for nutritionists and plant breeders in selecting suitable cultivars and genetically diverse parents for plant breeding programs
Acaulospora aspera, a new fungal species in the Glomeromycetes from rhizosphere soils of the inka nut (Plukenetia volubilis L.) in Peru
A new fungal species of the Glomeromycetes, Acaulospora aspera, was isolated from the rhizosphere of the inka nut (Plukenetia volubilis) in San Martín State of Peru (Western Amazonia) and propagated in bait cultures on Sorghum spp., Brachiaria brizantha, Medicago sativa and P. volubilis as host plants. The fungus forms brownish yellow to yellow brown spores, (120-)135-195 × (120-)130-187 μm in diameter. The surface of the structural spore wall layer is crowded with small depressions, 0.4-0.7 μm in diameter, up to 0.8 μm deep, and only 1.1-1.8 apart, giving the spore surface a rough, washboardlike appearance, especially when the outermost, evanescent wall layer has disappeared. Phylogenetically, the new species is close to A. spinosissima, A. excavata and to other morphologically more similar species such as A. spinosa and A. tuberculata, which form spiny or tuberculate projections on the outermost, semi-persistent spore wall layer, or A. herrerae, A. kentinensis, A. scrobiculata and A. minuta, which on the structural spore wall layer all have more pronunced pits than A. aspera. In this study, also the name of A. spinosissima was validated, as it had been preliminary declared invalid because of a typing error in the diagnosis section of its original description