Showa Women's University Repository / 昭和女子大学学術機関リポジトリ
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Special lecture: Empowering Managers, Empowering Woman
Get PDFメアリー・ブリントン（ハーバード大学）、武川恵子（昭和女子大学）、アンナ＝まりあ・ウィルヤネン（フィンランドセンター）、ダグラス・ハイマス（ニューヨークメロン銀行）、坂東眞理子（昭和女子大学）による講演・シンポジウムの記録departmental bulletin pape
The Export Trafficking of the Muscarinic Acetylcholine Receptors Induced by an Antagonist
Get PDFG protein-coupled receptors are cell-surface receptors, many of which have conserved motif F（x）6LL in their C-terminal intracellular region. The motif is known to function when the G protein-coupled receptors are exported from the endoplasmic reticulum to the cell surface. We reported previously that the amino acid mutations of the conserved leucines of M1 muscarinic acetylcholine receptor caused significant decrease in the cell-surface expression and significant increase in the endoplasmic reticulum expression of the mutant receptor, and that in the presence of antagonist atropine, the mutant receptor showed cell-surface expression, similar to the expression of the wild-type receptor. In this study, we investigated the export trafficking of the mutant receptor by the measurements of membrane-impermeable antagonist ［3H］N-methylscopolamine binding to the cell surface, and indicated that the cell-surface expression of the mutant receptor in the presence of atropine decreased to 20％ by the depletion of atropine for 3 days, and recovered to the same amount as before by the second addition of atropine. We also investigated the export trafficking of the mutant receptor using the total internal reflection fluorescence microscope, and indicated that the mutant receptor expressed time-dependently to the cell surface by the second addition of atropine.departmental bulletin pape
